ID   EXOC3_HUMAN             Reviewed;         745 AA.
AC   O60645; Q6P2E8; Q8TEN6; Q8WUW0; Q96DI4;
DT   25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 3.
DT   27-MAR-2024, entry version 180.
DE   RecName: Full=Exocyst complex component 3;
DE   AltName: Full=Exocyst complex component Sec6;
GN   Name=EXOC3; Synonyms=SEC6, SEC6L1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Epididymis;
RX   PubMed=20736409; DOI=10.1074/mcp.m110.001719;
RA   Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C.,
RA   Jin S., Liu J., Zhu P., Liu Y.;
RT   "Systematic mapping and functional analysis of a family of human epididymal
RT   secretory sperm-located proteins.";
RL   Mol. Cell. Proteomics 9:2517-2528(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=12693554; DOI=10.1093/dnares/10.1.49;
RA   Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N.,
RA   Ohara O.;
RT   "Characterization of long cDNA clones from human adult spleen. II. The
RT   complete sequences of 81 cDNA clones.";
RL   DNA Res. 10:49-57(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15372022; DOI=10.1038/nature02919;
RA   Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA   Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA   She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA   Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA   Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA   Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA   Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA   Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA   Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA   Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA   Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA   Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT   "The DNA sequence and comparative analysis of human chromosome 5.";
RL   Nature 431:268-274(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Colon, Lung, and Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-745.
RC   TISSUE=Brain;
RA   Yu W., Gibbs R.A.;
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   INTERACTION WITH BIRC6.
RX   PubMed=18329369; DOI=10.1016/j.cell.2008.01.012;
RA   Pohl C., Jentsch S.;
RT   "Final stages of cytokinesis and midbody ring formation are controlled by
RT   BRUCE.";
RL   Cell 132:832-845(2008).
RN   [8]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18756269; DOI=10.1038/emboj.2008.166;
RA   Cascone I., Selimoglu R., Ozdemir C., Del Nery E., Yeaman C., White M.,
RA   Camonis J.;
RT   "Distinct roles of RalA and RalB in the progression of cytokinesis are
RT   supported by distinct RalGEFs.";
RL   EMBO J. 27:2375-2387(2008).
RN   [9]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-28, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19608861; DOI=10.1126/science.1175371;
RA   Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA   Olsen J.V., Mann M.;
RT   "Lysine acetylation targets protein complexes and co-regulates major
RT   cellular functions.";
RL   Science 325:834-840(2009).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [11]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC       exocytic vesicles with fusion sites on the plasma membrane.
CC   -!- SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4,
CC       EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1
CC       (By similarity). Interacts with BIRC6/bruce. Interacts with MYRIP (By
CC       similarity). Interacts with SLC6A9 (By similarity).
CC       {ECO:0000250|UniProtKB:O54921, ECO:0000250|UniProtKB:Q62825,
CC       ECO:0000250|UniProtKB:Q6KAR6, ECO:0000269|PubMed:18329369}.
CC   -!- INTERACTION:
CC       O60645; A1L4K1: FSD2; NbExp=3; IntAct=EBI-1052278, EBI-5661036;
CC       O60645; Q96IK5: GMCL1; NbExp=3; IntAct=EBI-1052278, EBI-2548508;
CC       O60645; Q9BZL4: PPP1R12C; NbExp=3; IntAct=EBI-1052278, EBI-721802;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O54921}.
CC       Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O54921}. Cell
CC       projection, growth cone {ECO:0000250|UniProtKB:O54921}. Midbody
CC       {ECO:0000269|PubMed:18756269}. Golgi apparatus
CC       {ECO:0000305|PubMed:18756269}. Cell projection, neuron projection
CC       {ECO:0000250|UniProtKB:Q62825}. Note=Perinuclear in undifferentiated
CC       cells. Redistributes to growing neurites and growth cones during
CC       neuronal differentiation (By similarity). During mitosis, early
CC       recruitment to the midbody requires RALA, but not RALB, and EXOC2. In
CC       late stages of cytokinesis, localization to the midbody is RALB-
CC       dependent (PubMed:18756269). {ECO:0000250|UniProtKB:O54921,
CC       ECO:0000269|PubMed:18756269}.
CC   -!- TISSUE SPECIFICITY: Expressed in epididymis (at protein level).
CC       {ECO:0000269|PubMed:20736409}.
CC   -!- SIMILARITY: Belongs to the SEC6 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB84912.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; GU727644; ADU87645.1; -; mRNA.
DR   EMBL; AK074086; BAB84912.1; ALT_SEQ; mRNA.
DR   EMBL; AC010442; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471235; EAW51000.1; -; Genomic_DNA.
DR   EMBL; BC001511; AAH01511.2; -; mRNA.
DR   EMBL; BC019304; AAH19304.1; -; mRNA.
DR   EMBL; BC064569; AAH64569.1; -; mRNA.
DR   EMBL; AF055006; AAC09358.1; -; mRNA.
DR   CCDS; CCDS54830.1; -.
DR   RefSeq; NP_009208.2; NM_007277.4.
DR   RefSeq; XP_016864492.1; XM_017009003.1.
DR   RefSeq; XP_016864493.1; XM_017009004.1.
DR   AlphaFoldDB; O60645; -.
DR   SMR; O60645; -.
DR   BioGRID; 116464; 147.
DR   ComplexPortal; CPX-4943; Exocyst, EXOC6 variant.
DR   ComplexPortal; CPX-4944; Exocyst, EXOC6B variant.
DR   CORUM; O60645; -.
DR   IntAct; O60645; 52.
DR   MINT; O60645; -.
DR   STRING; 9606.ENSP00000425587; -.
DR   TCDB; 1.F.2.1.2; the octameric exocyst (exocyst) family.
DR   iPTMnet; O60645; -.
DR   PhosphoSitePlus; O60645; -.
DR   BioMuta; EXOC3; -.
DR   EPD; O60645; -.
DR   jPOST; O60645; -.
DR   MassIVE; O60645; -.
DR   MaxQB; O60645; -.
DR   PaxDb; 9606-ENSP00000425587; -.
DR   PeptideAtlas; O60645; -.
DR   ProteomicsDB; 66896; -.
DR   Pumba; O60645; -.
DR   Antibodypedia; 22230; 189 antibodies from 27 providers.
DR   DNASU; 11336; -.
DR   Ensembl; ENST00000315013.9; ENSP00000323377.5; ENSG00000180104.16.
DR   Ensembl; ENST00000512944.6; ENSP00000425587.1; ENSG00000180104.16.
DR   GeneID; 11336; -.
DR   KEGG; hsa:11336; -.
DR   MANE-Select; ENST00000512944.6; ENSP00000425587.1; NM_007277.5; NP_009208.2.
DR   UCSC; uc003jba.4; human.
DR   AGR; HGNC:30378; -.
DR   CTD; 11336; -.
DR   DisGeNET; 11336; -.
DR   GeneCards; EXOC3; -.
DR   HGNC; HGNC:30378; EXOC3.
DR   HPA; ENSG00000180104; Low tissue specificity.
DR   MIM; 608186; gene.
DR   neXtProt; NX_O60645; -.
DR   OpenTargets; ENSG00000180104; -.
DR   PharmGKB; PA134869816; -.
DR   VEuPathDB; HostDB:ENSG00000180104; -.
DR   eggNOG; KOG2286; Eukaryota.
DR   GeneTree; ENSGT01030000234613; -.
DR   HOGENOM; CLU_016260_1_0_1; -.
DR   InParanoid; O60645; -.
DR   OMA; MNIGPKT; -.
DR   OrthoDB; 5490183at2759; -.
DR   PhylomeDB; O60645; -.
DR   TreeFam; TF314979; -.
DR   PathwayCommons; O60645; -.
DR   Reactome; R-HSA-1445148; Translocation of SLC2A4 (GLUT4) to the plasma membrane.
DR   Reactome; R-HSA-264876; Insulin processing.
DR   Reactome; R-HSA-5620916; VxPx cargo-targeting to cilium.
DR   SignaLink; O60645; -.
DR   SIGNOR; O60645; -.
DR   BioGRID-ORCS; 11336; 574 hits in 1166 CRISPR screens.
DR   ChiTaRS; EXOC3; human.
DR   GeneWiki; EXOC3; -.
DR   GenomeRNAi; 11336; -.
DR   Pharos; O60645; Tbio.
DR   PRO; PR:O60645; -.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; O60645; Protein.
DR   Bgee; ENSG00000180104; Expressed in mucosa of transverse colon and 202 other cell types or tissues.
DR   ExpressionAtlas; O60645; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
DR   GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042734; C:presynaptic membrane; IEA:Ensembl.
DR   GO; GO:0030667; C:secretory granule membrane; TAS:Reactome.
DR   GO; GO:0045296; F:cadherin binding; HDA:BHF-UCL.
DR   GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR   GO; GO:0051601; P:exocyst localization; IBA:GO_Central.
DR   GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR   GO; GO:0090148; P:membrane fission; NAS:ComplexPortal.
DR   GO; GO:0000281; P:mitotic cytokinesis; NAS:ComplexPortal.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006904; P:vesicle docking involved in exocytosis; NAS:ComplexPortal.
DR   GO; GO:0090522; P:vesicle tethering involved in exocytosis; NAS:ComplexPortal.
DR   Gene3D; 1.10.357.50; -; 1.
DR   Gene3D; 1.10.357.70; Exocyst complex component Sec6, C-terminal domain; 1.
DR   InterPro; IPR010326; EXOC3/Sec6.
DR   InterPro; IPR042532; EXOC3/Sec6_C.
DR   PANTHER; PTHR21292:SF13; EXOCYST COMPLEX COMPONENT 3; 1.
DR   PANTHER; PTHR21292; EXOCYST COMPLEX COMPONENT SEC6-RELATED; 1.
DR   Pfam; PF06046; Sec6; 1.
DR   Genevisible; O60645; HS.
PE   1: Evidence at protein level;
KW   Acetylation; Cell projection; Cytoplasm; Exocytosis; Golgi apparatus;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..745
FT                   /note="Exocyst complex component 3"
FT                   /id="PRO_0000118925"
FT   MOD_RES         28
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
SQ   SEQUENCE   745 AA;  85567 MW;  81D03FB3351A995D CRC64;
     MKETDREAVA TAVQRVAGML QRPDQLDKVE QYRRREARKK ASVEARLKAA IQSQLDGVRT
     GLSQLHNALN DVKDIQQSLA DVSKDWRQSI NTIESLKDVK DAVVQHSQLA AAVENLKNIF
     SVPEIVRETQ DLIEQGALLQ AHRKLMDLEC SRDGLMYEQY RMDSGNTRDM TLIHGYFGST
     QGLSDELAKQ LWMVLQRSLV TVRRDPTLLV SVVRIIEREE KIDRRILDRK KQTGFVPPGR
     PKNWKEKMFT ILERTVTTRI EGTQADTRES DKMWLVRHLE IIRKYVLDDL IVAKNLMVQC
     FPPHYEIFKN LLNMYHQALS TRMQDLASED LEANEIVSLL TWVLNTYTST EMMRNVELAP
     EVDVGTLEPL LSPHVVSELL DTYMSTLTSN IIAWLRKALE TDKKDWVKET EPEADQDGYY
     QTTLPAIVFQ MFEQNLQVAA QISEDLKTKV LVLCLQQMNS FLSRYKDEAQ LYKEEHLRNR
     QHPHCYVQYM IAIINNCQTF KESIVSLKRK YLKNEVEEGV SPSQPSMDGI LDAIAKEGCS
     GLLEEVFLDL EQHLNELMTK KWLLGSNAVD IICVTVEDYF NDFAKIKKPY KKRMTAEAHR
     RVVVEYLRAV MQKRISFRSP EERKEGAEKM VREAEQLRFL FRKLASGFGE DVDGYCDTIV
     AVAEVIKLTD PSLLYLEVST LVSKYPDIRD DHIGALLAVR GDASRDMKQT IMETLEQGPA
     QASPSYVPLF KDIVVPSLNV AKLLK
//