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Protein

Clathrin coat assembly protein AP180

Gene

SNAP91

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • clathrin coat assembly Source: InterPro
  • protein transport Source: UniProtKB-KW
  • regulation of clathrin-dependent endocytosis Source: MGI

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180
Alternative name(s):
91 kDa synaptosomal-associated protein
Clathrin coat-associated protein AP180
Phosphoprotein F1-20
Gene namesi
Name:SNAP91
Synonyms:KIAA0656
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000065609.14
HGNCiHGNC:14986 SNAP91
MIMi607923 gene
neXtProtiNX_O60641

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9892
OpenTargetsiENSG00000065609
PharmGKBiPA37956

Polymorphism and mutation databases

BioMutaiSNAP91

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001938641 – 907Clathrin coat assembly protein AP180Add BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Glycosylationi310O-linked (GlcNAc) threonineBy similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei317PhosphothreonineBy similarity1
Modified residuei596PhosphoserineBy similarity1
Modified residuei602PhosphoserineBy similarity1
Modified residuei623PhosphoserineBy similarity1
Modified residuei629PhosphoserineBy similarity1
Modified residuei763PhosphoserineBy similarity1
Modified residuei865Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei865Omega-N-methylarginine; alternateBy similarity1

Post-translational modificationi

Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

MaxQBiO60641
PaxDbiO60641
PeptideAtlasiO60641
PRIDEiO60641
ProteomicsDBi49493
49494 [O60641-2]
49495 [O60641-3]

PTM databases

iPTMnetiO60641
PhosphoSitePlusiO60641
SwissPalmiO60641

Expressioni

Gene expression databases

BgeeiENSG00000065609
CleanExiHS_SNAP91
ExpressionAtlasiO60641 baseline and differential
GenevisibleiO60641 HS

Organism-specific databases

HPAiCAB009745
HPA029632
HPA029633

Interactioni

Subunit structurei

Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Necap1Q9CR952EBI-1105187,EBI-7592476From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115222, 11 interactors
ELMiO60641
IntActiO60641, 7 interactors
MINTiO60641
STRINGi9606.ENSP00000358708

Structurei

3D structure databases

ProteinModelPortaliO60641
SMRiO60641
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 145ENTHPROSITE-ProRule annotationAdd BLAST132

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi361 – 583Ala-richAdd BLAST223
Compositional biasi536 – 556Thr-richAdd BLAST21
Compositional biasi809 – 897Pro-richAdd BLAST89

Domaini

Possesses a three domain structure: the N-terminal 300 residues harbor a clathrin binding site, an acidic middle domain 450 residues, interrupted by an Ala-rich segment, and the C-terminal domain (166 residues).

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
GeneTreeiENSGT00390000008805
HOGENOMiHOG000015763
HOVERGENiHBG049391
InParanoidiO60641
KOiK20043
OMAiLMPTMAP
OrthoDBiEOG091G0810
PhylomeDBiO60641
TreeFamiTF314861

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60641-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMA PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDL FATASAAVPV STSKPSSDLL
360 370 380 390 400
DLQPDFSSGG AAAAAAPAPP PPAGGATAWG DLLGEDSLAA LSSVPSEAQI
410 420 430 440 450
SDPFAPEPTP PTTTAEIATA SASASTTTTV TAVTAEVDLF GDAFAASPGE
460 470 480 490 500
APAASEGAAA PATPTPVAAA LDACSGNDPF APSEGSAEAA PELDLFAMKP
510 520 530 540 550
PETSVPVVTP TASTAPPVPA TAPSPAPAVA AAAAATTAAT AAATTTTTTS
560 570 580 590 600
AATATTAPPA LDIFGDLFES TPEVAAAPKP DAAPSIDLFS TDAFSSPPQG
610 620 630 640 650
ASPVPESSLT ADLLSVDAFA APSPATTASP AKVDSSGVID LFGDAFGSSA
660 670 680 690 700
SEPQPASQAA SSSSASADLL AGFGGSFMAP SPSPVTPAQN NLLQPNFEAA
710 720 730 740 750
FGTTPSTSSS SSFDPSVFDG LGDLLMPTMA PAGQPAPVSM VPPSPAMAAS
760 770 780 790 800
KALGSDLDSS LASLVGNLGI SGTTTKKGDL QWNAGEKKLT GGANWQPKVA
810 820 830 840 850
PATWSAGVPP SAPLQGAVPP TSSVPPVAGA PSVGQPGAGF GMPPAGTGMP
860 870 880 890 900
MMPQQPVMFA QPMMRPPFGA AAVPGTQLSP SPTPASQSPK KPPAKDPLAD

LNIKDFL
Length:907
Mass (Da):92,502
Last modified:August 1, 1999 - v2
Checksum:i23959C2B54F5EBF1
GO
Isoform 2 (identifier: O60641-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-907: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:58
Mass (Da):6,230
Checksum:i22EBDA4093641147
GO
Isoform 3 (identifier: O60641-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-269: Missing.
     293-295: NEG → K
     381-671: Missing.

Show »
Length:600
Mass (Da):63,128
Checksum:i0B92B5140631B77F
GO
Isoform 4 (identifier: O60641-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     293-295: NEG → K
     616-643: Missing.

Note: Gene prediction based on EST data.
Show »
Length:877
Mass (Da):89,656
Checksum:iA9A46C044BE55582
GO

Sequence cautioni

The sequence BAA31631 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB89292 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAH18201 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4Q → R in AAH60818 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02099759 – 907Missing in isoform 2. 1 PublicationAdd BLAST849
Alternative sequenceiVSP_020998256 – 269Missing in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_020999293 – 295NEG → K in isoform 3 and isoform 4. 1 Publication3
Alternative sequenceiVSP_021000381 – 671Missing in isoform 3. 1 PublicationAdd BLAST291
Alternative sequenceiVSP_047049616 – 643Missing in isoform 4. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014556 mRNA Translation: BAA31631.2 Different initiation.
AK289582 mRNA Translation: BAF82271.1
CR749348 mRNA Translation: CAH18201.1 Sequence problems.
AL109915 Genomic DNA Translation: CAB89292.1 Sequence problems.
AL109915 Genomic DNA Translation: CAI19453.1
AL136972 Genomic DNA No translation available.
AF054993 mRNA Translation: AAC09352.1
BC060818 mRNA Translation: AAH60818.1
CCDSiCCDS47455.1 [O60641-1]
CCDS56437.1 [O60641-3]
CCDS56438.1 [O60641-4]
RefSeqiNP_001229721.1, NM_001242792.1 [O60641-1]
NP_001229722.1, NM_001242793.1 [O60641-4]
NP_001229723.1, NM_001242794.1 [O60641-3]
NP_001243646.1, NM_001256717.1
NP_001243647.1, NM_001256718.1
NP_055656.1, NM_014841.2 [O60641-1]
XP_005248827.1, XM_005248770.4 [O60641-1]
XP_006715678.1, XM_006715615.1 [O60641-1]
XP_011534567.1, XM_011536265.1 [O60641-1]
XP_011534568.1, XM_011536266.1 [O60641-1]
UniGeneiHs.368046

Genome annotation databases

EnsembliENST00000369694; ENSP00000358708; ENSG00000065609 [O60641-1]
ENST00000439399; ENSP00000400459; ENSG00000065609 [O60641-1]
ENST00000520213; ENSP00000428026; ENSG00000065609 [O60641-3]
ENST00000520302; ENSP00000428511; ENSG00000065609 [O60641-4]
ENST00000521743; ENSP00000428215; ENSG00000065609 [O60641-1]
GeneIDi9892
KEGGihsa:9892
UCSCiuc003pkc.4 human [O60641-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAP180_HUMAN
AccessioniPrimary (citable) accession number: O60641
Secondary accession number(s): A8K0L7
, E5RI02, Q5JX13, Q68DL9, Q6P9D3, Q9NTY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: August 1, 1999
Last modified: June 20, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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