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O60637 (TSN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tetraspanin-3

Short name=Tspan-3
Alternative name(s):
Tetraspanin TM4-A
Transmembrane 4 superfamily member 8
Gene names
Name:TSPAN3
Synonyms:TM4SF8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulates the proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair By similarity.

Subunit structure

Interacts with claudin-11/CLDN11 and integrins By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

integral component of membrane

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60637-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60637-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-110: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: O60637-3)

The sequence of this isoform differs from the canonical sequence as follows:
     22-85: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 253253Tetraspanin-3
PRO_0000219239

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 5018Extracellular Potential
Transmembrane51 – 7121Helical; Potential
Topological domain72 – 8514Cytoplasmic Potential
Transmembrane86 – 10621Helical; Potential
Topological domain107 – 212106Extracellular Potential
Transmembrane213 – 23321Helical; Potential
Topological domain234 – 25320Cytoplasmic Potential

Amino acid modifications

Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1831N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence22 – 8564Missing in isoform 3.
VSP_043519
Alternative sequence86 – 11025Missing in isoform 2.
VSP_042679

Experimental info

Sequence conflict851Missing in AAH00704. Ref.7
Sequence conflict2361R → G in BAA91613. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E074A4CD57229EC6

FASTA25328,018
        10         20         30         40         50         60 
MGQCGITSSK TVLVFLNLIF WGAAGILCYV GAYVFITYDD YDHFFEDVYT LIPAVVIIAV 

        70         80         90        100        110        120 
GALLFIIGLI GCCATIRESR CGLATFVIIL LLVFVTEVVV VVLGYVYRAK VENEVDRSIQ 

       130        140        150        160        170        180 
KVYKTYNGTN PDAASRAIDY VQRQLHCCGI HNYSDWENTD WFKETKNQSV PLSCCRETAS 

       190        200        210        220        230        240 
NCNGSLAHPS DLYAEGCEAL VVKKLQEIMM HVIWAALAFA AIQLLGMLCA CIVLCRRSRD 

       250 
PAYELLITGG TYA 

« Hide

Isoform 2 [UniParc].

Checksum: F44F7EAD2803A735
Show »

FASTA22825,183
Isoform 3 [UniParc].

Checksum: AD906FFB870A639D
Show »

FASTA18921,184

References

« Hide 'large scale' references
[1]"Sequences and expression of six new members of the tetraspanin/TM4SF family."
Todd S.C., Doctor V.S., Levy S.
Biochim. Biophys. Acta 1399:101-104(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The molecular characterization of four tetraspanins."
Puls K.L., Ni J., Liu D., Morahan G., Wright M.D.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Ovary.
[5]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cervix, Colon, Muscle and Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF054840 mRNA. Translation: AAC69716.1.
AF133423 mRNA. Translation: AAF08362.1.
AK001326 mRNA. Translation: BAA91627.1.
AK001305 mRNA. Translation: BAA91613.1.
AK001310 mRNA. Translation: BAA91615.1.
AK075336 mRNA. Translation: BAG52114.1.
AK075482 mRNA. Translation: BAG52152.1.
AK298157 mRNA. Translation: BAG60431.1.
AC090181 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99209.1.
CH471136 Genomic DNA. Translation: EAW99210.1.
CH471136 Genomic DNA. Translation: EAW99211.1.
BC000704 mRNA. Translation: AAH00704.1.
BC004280 mRNA. Translation: AAH04280.1.
BC009248 mRNA. Translation: AAH09248.1.
BC011206 mRNA. Translation: AAH11206.1.
CCDSCCDS10292.1. [O60637-1]
CCDS10293.1. [O60637-2]
CCDS53963.1. [O60637-3]
PIRA59264.
RefSeqNP_001161884.1. NM_001168412.1. [O60637-3]
NP_005715.1. NM_005724.5. [O60637-1]
NP_944492.1. NM_198902.2. [O60637-2]
UniGeneHs.744863.

3D structure databases

ProteinModelPortalO60637.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115406. 3 interactions.
IntActO60637. 3 interactions.
MINTMINT-1465751.
STRING9606.ENSP00000267970.

PTM databases

PhosphoSiteO60637.

Proteomic databases

MaxQBO60637.
PaxDbO60637.
PRIDEO60637.

Protocols and materials databases

DNASU10099.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000267970; ENSP00000267970; ENSG00000140391. [O60637-1]
ENST00000346495; ENSP00000341329; ENSG00000140391. [O60637-2]
ENST00000424443; ENSP00000407243; ENSG00000140391. [O60637-3]
GeneID10099.
KEGGhsa:10099.
UCSCuc002bcj.3. human. [O60637-1]
uc002bck.3. human. [O60637-2]
uc010ump.2. human. [O60637-3]

Organism-specific databases

CTD10099.
GeneCardsGC15M077336.
HGNCHGNC:17752. TSPAN3.
HPAHPA015996.
MIM613134. gene.
neXtProtNX_O60637.
PharmGKBPA134913500.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG317438.
HOGENOMHOG000230654.
HOVERGENHBG104967.
InParanoidO60637.
KOK17293.
OMAYVIWAAL.
OrthoDBEOG7PGDRZ.
PhylomeDBO60637.
TreeFamTF316345.

Gene expression databases

ArrayExpressO60637.
BgeeO60637.
CleanExHS_TSPAN3.
GenevestigatorO60637.

Family and domain databases

InterProIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
PRINTSPR00259. TMFOUR.
SUPFAMSSF48652. SSF48652. 1 hit.
PROSITEPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTSPAN3. human.
GeneWikiTSPAN3.
GenomeRNAi10099.
NextBio38199.
PROO60637.
SOURCESearch...

Entry information

Entry nameTSN3_HUMAN
AccessionPrimary (citable) accession number: O60637
Secondary accession number(s): A6NEH4 expand/collapse secondary AC list , B3KQQ2, B4DP19, Q9BW22, Q9NVX9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM