O60637 (TSN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraspanin-3
Short name=Tspan-3

Alternative name(s):
Tetraspanin TM4-A
Transmembrane 4 superfamily member 8

Gene names

Name:	TSPAN3
Synonyms:	TM4SF8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulates the proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair By similarity.
Subunit structure	Interacts with claudin-11/CLDN11 and integrins By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60637-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60637-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-110: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: O60637-3)

The sequence of this isoform differs from the canonical sequence as follows:
     22-85: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Tetraspanin-3		PRO_0000219239
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 50	18	Extracellular Potential
	Transmembrane	51 – 71	21	Helical; Potential
	Topological domain	72 – 85	14	Cytoplasmic Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Topological domain	107 – 212	106	Extracellular Potential
	Transmembrane	213 – 233	21	Helical; Potential
	Topological domain	234 – 253	20	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	22 – 85	64	Missing in isoform 3.		VSP_043519
	Alternative sequence	86 – 110	25	Missing in isoform 2.		VSP_042679
Experimental info
	Sequence conflict	85	1	Missing in AAH00704. Ref.7
	Sequence conflict	236	1	R → G in BAA91613. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E074A4CD57229EC6	FASTA	253	28,018
	10 20 30 40 50 60 MGQCGITSSK TVLVFLNLIF WGAAGILCYV GAYVFITYDD YDHFFEDVYT LIPAVVIIAV 70 80 90 100 110 120 GALLFIIGLI GCCATIRESR CGLATFVIIL LLVFVTEVVV VVLGYVYRAK VENEVDRSIQ 130 140 150 160 170 180 KVYKTYNGTN PDAASRAIDY VQRQLHCCGI HNYSDWENTD WFKETKNQSV PLSCCRETAS 190 200 210 220 230 240 NCNGSLAHPS DLYAEGCEAL VVKKLQEIMM HVIWAALAFA AIQLLGMLCA CIVLCRRSRD 250 PAYELLITGG TYA
	Isoform 2 [UniParc]. Checksum: F44F7EAD2803A735	FASTA	228	25,183
	10 20 30 40 50 60 MGQCGITSSK TVLVFLNLIF WGAAGILCYV GAYVFITYDD YDHFFEDVYT LIPAVVIIAV 70 80 90 100 110 120 GALLFIIGLI GCCATIRESR CGLATVENEV DRSIQKVYKT YNGTNPDAAS RAIDYVQRQL 130 140 150 160 170 180 HCCGIHNYSD WENTDWFKET KNQSVPLSCC RETASNCNGS LAHPSDLYAE GCEALVVKKL 190 200 210 220 QEIMMHVIWA ALAFAAIQLL GMLCACIVLC RRSRDPAYEL LITGGTYA
	Isoform 3 [UniParc]. Checksum: AD906FFB870A639D	FASTA	189	21,184
	10 20 30 40 50 60 MGQCGITSSK TVLVFLNLIF WFVIILLLVF VTEVVVVVLG YVYRAKVENE VDRSIQKVYK 70 80 90 100 110 120 TYNGTNPDAA SRAIDYVQRQ LHCCGIHNYS DWENTDWFKE TKNQSVPLSC CRETASNCNG 130 140 150 160 170 180 SLAHPSDLYA EGCEALVVKK LQEIMMHVIW AALAFAAIQL LGMLCACIVL CRRSRDPAYE LLITGGTYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences and expression of six new members of the tetraspanin/TM4SF family." Todd S.C., Doctor V.S., Levy S. Biochim. Biophys. Acta 1399:101-104(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The molecular characterization of four tetraspanins." Puls K.L., Ni J., Liu D., Morahan G., Wright M.D. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Ovary.
[5]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix, Colon, Muscle and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF054840 mRNA. Translation: AAC69716.1. AF133423 mRNA. Translation: AAF08362.1. AK001326 mRNA. Translation: BAA91627.1. AK001305 mRNA. Translation: BAA91613.1. AK001310 mRNA. Translation: BAA91615.1. AK075336 mRNA. Translation: BAG52114.1. AK075482 mRNA. Translation: BAG52152.1. AK298157 mRNA. Translation: BAG60431.1. AC090181 Genomic DNA. No translation available. CH471136 Genomic DNA. Translation: EAW99209.1. CH471136 Genomic DNA. Translation: EAW99210.1. CH471136 Genomic DNA. Translation: EAW99211.1. BC000704 mRNA. Translation: AAH00704.1. BC004280 mRNA. Translation: AAH04280.1. BC009248 mRNA. Translation: AAH09248.1. BC011206 mRNA. Translation: AAH11206.1.
IPI	IPI00030941. IPI00396249. IPI00910611.
PIR	A59264.
RefSeq	NP_001161884.1. NM_001168412.1. NP_005715.1. NM_005724.5. NP_944492.1. NM_198902.2.
UniGene	Hs.5062.
3D structure databases
ProteinModelPortal	O60637.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60637. 3 interactions.
MINT	MINT-1465751.
STRING	9606.ENSP00000267970.
PTM databases
PhosphoSite	O60637.
Proteomic databases
PaxDb	O60637.
PRIDE	O60637.
Protocols and materials databases
DNASU	10099.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267970; ENSP00000267970; ENSG00000140391. ENST00000346495; ENSP00000341329; ENSG00000140391. ENST00000424443; ENSP00000407243; ENSG00000140391.
GeneID	10099.
KEGG	hsa:10099.
UCSC	uc002bcj.3. human.
Organism-specific databases
CTD	10099.
GeneCards	GC15M077336.
HGNC	HGNC:17752. TSPAN3.
HPA	HPA015996.
MIM	613134. gene.
neXtProt	NX_O60637.
PharmGKB	PA134913500.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG317438.
HOGENOM	HOG000230654.
HOVERGEN	HBG104967.
InParanoid	O60637.
OMA	YVIWAAL.
OrthoDB	EOG4NCMDH.
PhylomeDB	O60637.
Gene expression databases
ArrayExpress	O60637.
Bgee	O60637.
CleanEx	HS_TSPAN3.
Genevestigator	O60637.
GermOnline	ENSG00000140391. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TSPAN3. human.
GenomeRNAi	10099.
NextBio	38199.
SOURCE	Search...

Entry information

Entry name

TSN3_HUMAN

Accession

Primary (citable) accession number: O60637
Secondary accession number(s): A6NEH4 , B3KQQ2, B4DP19, Q9BW22, Q9NVX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.