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O60635 (TSN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tetraspanin-1

Short name=Tspan-1
Alternative name(s):
Tetraspan NET-1
Tetraspanin TM4-C
Gene names
Name:TSPAN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Lysosome membrane; Multi-pass membrane protein Ref.8.

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
   Cellular componentLysosome
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Traceable author statement Ref.1. Source: ProtInc

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241Tetraspanin-1
PRO_0000219234

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 5220Extracellular Potential
Transmembrane53 – 7321Helical; Potential
Topological domain74 – 8815Cytoplasmic Potential
Transmembrane89 – 10921Helical; Potential
Topological domain110 – 211102Extracellular Potential
Transmembrane212 – 23221Helical; Potential
Topological domain233 – 2419Cytoplasmic Potential

Amino acid modifications

Glycosylation1411N-linked (GlcNAc...) Ref.8
Glycosylation1541N-linked (GlcNAc...) Ref.8
Glycosylation1781N-linked (GlcNAc...) Ref.8
Glycosylation1841N-linked (GlcNAc...) Ref.8

Natural variations

Natural variant381S → F.
Corresponds to variant rs2234267 [ dbSNP | Ensembl ].
VAR_052327
Natural variant871V → M.
Corresponds to variant rs2234268 [ dbSNP | Ensembl ].
VAR_034573

Experimental info

Mutagenesis1411N → A: Loss of glycosylation. Ref.8
Mutagenesis1541N → A: Loss of glycosylation. Ref.8
Mutagenesis1781N → A: Loss of glycosylation. Ref.8
Mutagenesis1841N → A: Loss of glycosylation. Ref.8
Sequence conflict1891K → E in AAC69714. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O60635 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: AF938AD7147CB884

FASTA24126,301
        10         20         30         40         50         60 
MQCFSFIKTM MILFNLLIFL CGAALLAVGI WVSIDGASFL KIFGPLSSSA MQFVNVGYFL 

        70         80         90        100        110        120 
IAAGVVVFAL GFLGCYGAKT ESKCALVTFF FILLLIFIAE VAAAVVALVY TTMAEHFLTL 

       130        140        150        160        170        180 
LVVPAIKKDY GSQEDFTQVW NTTMKGLKCC GFTNYTDFED SPYFKENSAF PPFCCNDNVT 

       190        200        210        220        230        240 
NTANETCTKQ KAHDQKVEGC FNQLLYDIRT NAVTVGGVAA GIGGLELAAM IVSMYLYCNL 


Q 

« Hide

References

« Hide 'large scale' references
[1]"Sequences and expression of six new members of the tetraspanin/TM4SF family."
Todd S.C., Doctor V.S., Levy S.
Biochim. Biophys. Acta 1399:101-104(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"New tetraspans identified in the EST database."
Rubinstein E., Serru V., Boucheix C.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The molecular characterization of four tetraspanins."
Puls K.L., Ni J., Liu D., Morahan G., Wright M.D.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[8]"Glycosylation of tetraspanin Tspan-1 at four distinct sites promotes its transition through the endoplasmic reticulum."
Scholz C.-J., Sauer G., Deissler H.
Protein Pept. Lett. 16:1244-1248(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-141; ASN-154; ASN-178 AND ASN-184, MUTAGENESIS OF ASN-141; ASN-154; ASN-178 AND ASN-184.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF054838 mRNA. Translation: AAC69714.1.
AF065388 mRNA. Translation: AAC17119.1.
AF133425 mRNA. Translation: AAF08364.1.
AK313774 mRNA. Translation: BAG36512.1.
AL672043 Genomic DNA. Translation: CAH72468.1.
CH471059 Genomic DNA. Translation: EAX06938.1.
CH471059 Genomic DNA. Translation: EAX06939.1.
CH471059 Genomic DNA. Translation: EAX06940.1.
BC007290 mRNA. Translation: AAH07290.1.
BC013404 mRNA. Translation: AAH13404.1.
IPIIPI00030936.
PIRA59262.
RefSeqNP_005718.2. NM_005727.3.
UniGeneHs.38972.

3D structure databases

ProteinModelPortalO60635.
ModBaseSearch...

Protein-protein interaction databases

IntActO60635. 1 interaction.
STRING9606.ENSP00000236130.

Proteomic databases

PaxDbO60635.
PeptideAtlasO60635.
PRIDEO60635.

Protocols and materials databases

DNASU10103.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000372003; ENSP00000361072; ENSG00000117472.
GeneID10103.
KEGGhsa:10103.
UCSCuc001cpd.3. human.

Organism-specific databases

CTD10103.
GeneCardsGC01P046640.
HGNCHGNC:20657. TSPAN1.
MIM613170. gene.
neXtProtNX_O60635.
PharmGKBPA134938100.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG281528.
HOGENOMHOG000230651.
HOVERGENHBG107159.
InParanoidO60635.
OMAGLKCCGF.
OrthoDBEOG4T4CWZ.
PhylomeDBO60635.

Gene expression databases

BgeeO60635.
CleanExHS_TSPAN1.
GenevestigatorO60635.
GermOnlineENSG00000117472. Homo sapiens.

Family and domain databases

InterProIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
PRINTSPR00259. TMFOUR.
SUPFAMSSF48652. Tetraspanin. 1 hit.
PROSITEPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10103.
NextBio38217.
SOURCESearch...

Entry information

Entry nameTSN1_HUMAN
AccessionPrimary (citable) accession number: O60635
Secondary accession number(s): D3DQ14, O60745, Q5VST0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 3, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families