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O60609

- GFRA3_HUMAN

UniProt

O60609 - GFRA3_HUMAN

Protein

GDNF family receptor alpha-3

Gene

GFRA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.1 Publication

    GO - Molecular functioni

    1. axon guidance receptor activity Source: Ensembl
    2. receptor binding Source: ProtInc

    GO - Biological processi

    1. nervous system development Source: ProtInc
    2. neuron migration Source: Ensembl
    3. peripheral nervous system development Source: ProtInc
    4. signal transduction Source: ProtInc
    5. sympathetic nervous system development Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDNF family receptor alpha-3
    Short name:
    GDNF receptor alpha-3
    Short name:
    GDNFR-alpha-3
    Short name:
    GFR-alpha-3
    Gene namesi
    Name:GFRA3
    ORF Names:UNQ339/PRO538/PRO3664
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:4245. GFRA3.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cytoplasm Source: HPA
    3. external side of plasma membrane Source: Ensembl
    4. extrinsic component of membrane Source: ProtInc
    5. intracellular membrane-bounded organelle Source: HPA
    6. plasma membrane Source: HPA

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28655.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 31311 PublicationAdd
    BLAST
    Chaini32 – 374343GDNF family receptor alpha-3PRO_0000010789Add
    BLAST
    Propeptidei375 – 40026Removed in mature formSequence AnalysisPRO_0000010790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)1 Publication
    Lipidationi374 – 3741GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiO60609.
    PRIDEiO60609.

    PTM databases

    PhosphoSiteiO60609.

    Expressioni

    Tissue specificityi

    Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.1 Publication

    Gene expression databases

    ArrayExpressiO60609.
    BgeeiO60609.
    CleanExiHS_GFRA3.
    GenevestigatoriO60609.

    Organism-specific databases

    HPAiHPA020731.

    Interactioni

    Protein-protein interaction databases

    BioGridi108944. 1 interaction.
    DIPiDIP-29114N.
    STRINGi9606.ENSP00000274721.

    Structurei

    Secondary structure

    1
    400
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi161 – 17010
    Helixi173 – 18513
    Turni188 – 1903
    Helixi193 – 20614
    Helixi209 – 2168
    Helixi225 – 2339
    Helixi237 – 2404
    Helixi248 – 2569
    Helixi259 – 27113
    Beta strandi274 – 2763
    Beta strandi281 – 2833
    Helixi285 – 2939
    Turni294 – 2974
    Beta strandi302 – 3043
    Beta strandi307 – 3093
    Beta strandi312 – 3154
    Helixi322 – 3243
    Helixi325 – 33612
    Helixi339 – 35517

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GH0X-ray1.92A/B151-363[»]
    ProteinModelPortaliO60609.
    SMRiO60609. Positions 158-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO60609.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GDNFR family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG83952.
    HOGENOMiHOG000059598.
    HOVERGENiHBG051726.
    InParanoidiO60609.
    OMAiQLFSQDW.
    OrthoDBiEOG7FBRJ3.
    PhylomeDBiO60609.
    TreeFamiTF331647.

    Family and domain databases

    InterProiIPR016017. GDNF/GAS1.
    IPR003438. GDNF_rcpt.
    IPR003505. GDNF_rcpt_A3.
    [Graphical view]
    PfamiPF02351. GDNF. 3 hits.
    [Graphical view]
    PRINTSiPR01319. GDNFRALPHA3.
    PR01316. GDNFRECEPTOR.
    SMARTiSM00907. GDNF. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O60609-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVRPLNPRPL PPVVLMLLLL LPPSPLPLAA GDPLPTESRL MNSCLQARRK    50
    CQADPTCSAA YHHLDSCTSS ISTPLPSEEP SVPADCLEAA QQLRNSSLIG 100
    CMCHRRMKNQ VACLDIYWTV HRARSLGNYE LDVSPYEDTV TSKPWKMNLS 150
    KLNMLKPDSD LCLKFAMLCT LNDKCDRLRK AYGEACSGPH CQRHVCLRQL 200
    LTFFEKAAEP HAQGLLLCPC APNDRGCGER RRNTIAPNCA LPPVAPNCLE 250
    LRRLCFSDPL CRSRLVDFQT HCHPMDILGT CATEQSRCLR AYLGLIGTAM 300
    TPNFVSNVNT SVALSCTCRG SGNLQEECEM LEGFFSHNPC LTEAIAAKMR 350
    FHSQLFSQDW PHPTFAVMAH QNENPAVRPQ PWVPSLFSCT LPLILLLSLW 400
    Length:400
    Mass (Da):44,511
    Last modified:July 19, 2004 - v2
    Checksum:iB0BC252FE1F072C7
    GO
    Isoform 2 (identifier: O60609-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         127-157: Missing.

    Show »
    Length:369
    Mass (Da):40,930
    Checksum:i43DEAB2E6B900CE3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081K → R in AAC24355. (PubMed:9576965)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei127 – 15731Missing in isoform 2. 2 PublicationsVSP_010942Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051767 mRNA. Translation: AAC24355.1.
    AY358997 mRNA. Translation: AAQ89356.1.
    AY359037 mRNA. Translation: AAQ89396.1.
    AK297693 mRNA. Translation: BAG60051.1.
    AK314022 mRNA. Translation: BAG36733.1.
    CH471062 Genomic DNA. Translation: EAW62152.1.
    CH471062 Genomic DNA. Translation: EAW62153.1.
    BC037951 mRNA. Translation: AAH37951.1.
    CCDSiCCDS4201.1. [O60609-1]
    RefSeqiNP_001487.2. NM_001496.3. [O60609-1]
    UniGeneiHs.58042.

    Genome annotation databases

    EnsembliENST00000274721; ENSP00000274721; ENSG00000146013. [O60609-1]
    ENST00000378362; ENSP00000367613; ENSG00000146013. [O60609-2]
    GeneIDi2676.
    KEGGihsa:2676.
    UCSCiuc003lcn.3. human. [O60609-1]
    uc003lco.3. human. [O60609-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051767 mRNA. Translation: AAC24355.1 .
    AY358997 mRNA. Translation: AAQ89356.1 .
    AY359037 mRNA. Translation: AAQ89396.1 .
    AK297693 mRNA. Translation: BAG60051.1 .
    AK314022 mRNA. Translation: BAG36733.1 .
    CH471062 Genomic DNA. Translation: EAW62152.1 .
    CH471062 Genomic DNA. Translation: EAW62153.1 .
    BC037951 mRNA. Translation: AAH37951.1 .
    CCDSi CCDS4201.1. [O60609-1 ]
    RefSeqi NP_001487.2. NM_001496.3. [O60609-1 ]
    UniGenei Hs.58042.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GH0 X-ray 1.92 A/B 151-363 [» ]
    ProteinModelPortali O60609.
    SMRi O60609. Positions 158-357.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108944. 1 interaction.
    DIPi DIP-29114N.
    STRINGi 9606.ENSP00000274721.

    PTM databases

    PhosphoSitei O60609.

    Proteomic databases

    PaxDbi O60609.
    PRIDEi O60609.

    Protocols and materials databases

    DNASUi 2676.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274721 ; ENSP00000274721 ; ENSG00000146013 . [O60609-1 ]
    ENST00000378362 ; ENSP00000367613 ; ENSG00000146013 . [O60609-2 ]
    GeneIDi 2676.
    KEGGi hsa:2676.
    UCSCi uc003lcn.3. human. [O60609-1 ]
    uc003lco.3. human. [O60609-2 ]

    Organism-specific databases

    CTDi 2676.
    GeneCardsi GC05M137588.
    HGNCi HGNC:4245. GFRA3.
    HPAi HPA020731.
    MIMi 605710. gene.
    neXtProti NX_O60609.
    PharmGKBi PA28655.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG83952.
    HOGENOMi HOG000059598.
    HOVERGENi HBG051726.
    InParanoidi O60609.
    OMAi QLFSQDW.
    OrthoDBi EOG7FBRJ3.
    PhylomeDBi O60609.
    TreeFami TF331647.

    Miscellaneous databases

    EvolutionaryTracei O60609.
    GeneWikii GFRA3.
    GenomeRNAii 2676.
    NextBioi 10566.
    PROi O60609.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60609.
    Bgeei O60609.
    CleanExi HS_GFRA3.
    Genevestigatori O60609.

    Family and domain databases

    InterProi IPR016017. GDNF/GAS1.
    IPR003438. GDNF_rcpt.
    IPR003505. GDNF_rcpt_A3.
    [Graphical view ]
    Pfami PF02351. GDNF. 3 hits.
    [Graphical view ]
    PRINTSi PR01319. GDNFRALPHA3.
    PR01316. GDNFRECEPTOR.
    SMARTi SM00907. GDNF. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung and Substantia nigra.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Pancreas.
    6. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 32-46.
    7. "Artemin, a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFRalpha3-RET receptor complex."
      Baloh R.H., Tansey M.G., Lampe P.A., Fahrner T.J., Enomoto H., Simburger K.S., Leitner M.L., Araki T., Johnson E.M. Jr., Milbrandt J.
      Neuron 21:1291-1302(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Structure of artemin complexed with its receptor GFRalpha3: convergent recognition of glial cell line-derived neurotrophic factors."
      Wang X., Baloh R.H., Milbrandt J., Garcia K.C.
      Structure 14:1083-1092(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 151-363 IN COMPLEX WITH ARTN, GLYCOSYLATION AT ASN-309.

    Entry informationi

    Entry nameiGFRA3_HUMAN
    AccessioniPrimary (citable) accession number: O60609
    Secondary accession number(s): B2RA36
    , B4DMY9, Q6UW20, Q8IUZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3