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O60609 (GFRA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GDNF family receptor alpha-3

Short name=GDNF receptor alpha-3
Short name=GDNFR-alpha-3
Short name=GFR-alpha-3
Gene names
Name:GFRA3
ORF Names:UNQ339/PRO538/PRO3664
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Ref.7

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Tissue specificity

Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system. Ref.1

Post-translational modification

N-glycosylated. Ref.8

Sequence similarities

Belongs to the GDNFR family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60609-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60609-2)

The sequence of this isoform differs from the canonical sequence as follows:
     127-157: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Ref.6
Chain32 – 374343GDNF family receptor alpha-3
PRO_0000010789
Propeptide375 – 40026Removed in mature form Potential
PRO_0000010790

Amino acid modifications

Lipidation3741GPI-anchor amidated asparagine Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Ref.8

Natural variations

Alternative sequence127 – 15731Missing in isoform 2.
VSP_010942

Experimental info

Sequence conflict1081K → R in AAC24355. Ref.1

Secondary structure

..................................... 400
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: B0BC252FE1F072C7

FASTA40044,511
        10         20         30         40         50         60 
MVRPLNPRPL PPVVLMLLLL LPPSPLPLAA GDPLPTESRL MNSCLQARRK CQADPTCSAA 

        70         80         90        100        110        120 
YHHLDSCTSS ISTPLPSEEP SVPADCLEAA QQLRNSSLIG CMCHRRMKNQ VACLDIYWTV 

       130        140        150        160        170        180 
HRARSLGNYE LDVSPYEDTV TSKPWKMNLS KLNMLKPDSD LCLKFAMLCT LNDKCDRLRK 

       190        200        210        220        230        240 
AYGEACSGPH CQRHVCLRQL LTFFEKAAEP HAQGLLLCPC APNDRGCGER RRNTIAPNCA 

       250        260        270        280        290        300 
LPPVAPNCLE LRRLCFSDPL CRSRLVDFQT HCHPMDILGT CATEQSRCLR AYLGLIGTAM 

       310        320        330        340        350        360 
TPNFVSNVNT SVALSCTCRG SGNLQEECEM LEGFFSHNPC LTEAIAAKMR FHSQLFSQDW 

       370        380        390        400 
PHPTFAVMAH QNENPAVRPQ PWVPSLFSCT LPLILLLSLW 

« Hide

Isoform 2 [UniParc].

Checksum: 43DEAB2E6B900CE3
Show »

FASTA36940,930

References

« Hide 'large scale' references
[1]"GFRalpha3 is an orphan member of the GDNF/neurturin/persephin receptor family."
Baloh R.H., Gorodinsky A., Golden J.P., Tansey M.G., Keck C.L., Popescu N.C., Johnson E.M. Jr., Milbrandt J.
Proc. Natl. Acad. Sci. U.S.A. 95:5801-5806(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Lung and Substantia nigra.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pancreas.
[6]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-46.
[7]"Artemin, a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFRalpha3-RET receptor complex."
Baloh R.H., Tansey M.G., Lampe P.A., Fahrner T.J., Enomoto H., Simburger K.S., Leitner M.L., Araki T., Johnson E.M. Jr., Milbrandt J.
Neuron 21:1291-1302(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Structure of artemin complexed with its receptor GFRalpha3: convergent recognition of glial cell line-derived neurotrophic factors."
Wang X., Baloh R.H., Milbrandt J., Garcia K.C.
Structure 14:1083-1092(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 151-363 IN COMPLEX WITH ARTN, GLYCOSYLATION AT ASN-309.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF051767 mRNA. Translation: AAC24355.1.
AY358997 mRNA. Translation: AAQ89356.1.
AY359037 mRNA. Translation: AAQ89396.1.
AK297693 mRNA. Translation: BAG60051.1.
AK314022 mRNA. Translation: BAG36733.1.
CH471062 Genomic DNA. Translation: EAW62152.1.
CH471062 Genomic DNA. Translation: EAW62153.1.
BC037951 mRNA. Translation: AAH37951.1.
RefSeqNP_001487.2. NM_001496.3.
UniGeneHs.58042.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GH0X-ray1.92A/B151-363[»]
ProteinModelPortalO60609.
SMRO60609. Positions 42-98, 158-357.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108944. 1 interaction.
DIPDIP-29114N.
STRING9606.ENSP00000274721.

PTM databases

PhosphoSiteO60609.

Proteomic databases

PaxDbO60609.
PRIDEO60609.

Protocols and materials databases

DNASU2676.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274721; ENSP00000274721; ENSG00000146013. [O60609-1]
ENST00000378362; ENSP00000367613; ENSG00000146013. [O60609-2]
GeneID2676.
KEGGhsa:2676.
UCSCuc003lcn.3. human. [O60609-1]
uc003lco.3. human. [O60609-2]

Organism-specific databases

CTD2676.
GeneCardsGC05M137588.
HGNCHGNC:4245. GFRA3.
HPAHPA020731.
MIM605710. gene.
neXtProtNX_O60609.
PharmGKBPA28655.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG83952.
HOGENOMHOG000059598.
HOVERGENHBG051726.
InParanoidO60609.
OMAQLFSQDW.
OrthoDBEOG7FBRJ3.
PhylomeDBO60609.
TreeFamTF331647.

Gene expression databases

ArrayExpressO60609.
BgeeO60609.
CleanExHS_GFRA3.
GenevestigatorO60609.

Family and domain databases

InterProIPR016017. GDNF/GAS1.
IPR003438. GDNF_rcpt.
IPR003505. GDNF_rcpt_A3.
[Graphical view]
PfamPF02351. GDNF. 3 hits.
[Graphical view]
PRINTSPR01319. GDNFRALPHA3.
PR01316. GDNFRECEPTOR.
SMARTSM00907. GDNF. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO60609.
GeneWikiGFRA3.
GenomeRNAi2676.
NextBio10566.
PROO60609.
SOURCESearch...

Entry information

Entry nameGFRA3_HUMAN
AccessionPrimary (citable) accession number: O60609
Secondary accession number(s): B2RA36 expand/collapse secondary AC list , B4DMY9, Q6UW20, Q8IUZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM