Reviewed, UniProtKB/Swiss-Prot O60573 (IF4E2_HUMAN)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 4E type 2
      Short name=eIF-4E type 2
      Short name=eIF4E type 2
Alternative name(s):
    mRNA cap-binding protein type 3
    Eukaryotic translation initiation factor 4E-like 3
    Eukaryotic translation initiation factor 4E homologous protein
    mRNA cap-binding protein 4EHP
    eIF4E-like protein 4E-LP

Gene names

Name:	EIF4E2
Synonyms:	EIF4EL3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Subunit structure	eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners By similarity. EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G.
Sequence similarities	Belongs to the eukaryotic initiation factor 4E family.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Ligand	RNA-binding
Molecular function	Initiation factor
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW translational initiation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	RNA cap binding Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF4EBP1	Q13541	1	EBI-398610,EBI-74090
TRAF6	Q9Y4K3	1	EBI-398610,EBI-359276

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 245

245

Eukaryotic translation initiation factor 4E type 2

PRO_0000193664

Experimental info

Mutagenesis

63

1

W → A: Unable to bind capped mRNA.

Mutagenesis

95

1

W → A: Ability to bind capped mRNA reduced to 40% of wild-type.

Mutagenesis

124 – 126

3

WED → FAA: Unable to bind capped mRNA.

Mutagenesis

124

1

W → A: Ability to bind capped mRNA reduced to less than 10% of wild-type.

Mutagenesis

124

1

W → F: Ability to bind capped mRNA reduced to 13% of wild-type.

Mutagenesis

125

1

E → A: Ability to bind capped mRNA reduced to less than 10% of wild-type.

Mutagenesis

126

1

D → A: Slight reduction in ability to bind capped mRNA.

Mutagenesis

135

1

W → A: Unable to bind capped mRNA.

Mutagenesis

148

1

W → A: Unable to bind capped mRNA.

Mutagenesis

183

1

W → A: Ability to bind capped mRNA reduced to less than 10% of wild-type.

Mutagenesis

183

1

W → F: Unable to bind capped mRNA.

Sequence conflict

1 – 27

27

MNNKF…STQKD → MMTVGTMIRMKKTAHRKI Ref.3

Secondary structure

1

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245

Helix Strand Turn

Details...

Beta strand

55 – 67

13

Helix

75 – 81

7

Beta strand

82 – 90

9

Helix

91 – 98

8

Helix

104 – 106

3

Beta strand

109 – 117

9

Turn

127 – 131

5

Beta strand

133 – 139

7

Helix

144 – 156

13

Beta strand

166 – 173

8

Beta strand

178 – 185

8

Helix

190 – 203

14

Beta strand

212 – 216

5

Helix

217 – 222

6

Sequences

	Sequence		Length	Mass (Da)
	O60573-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 3D3075BFA48B3C12	FASTA	245	28,362
	10 20 30 40 50 60 MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTERDKNQS SSKRKAVVPG PAEHPLQYNY 70 80 90 100 110 120 TFWYSRRTPG RPTSSQSYEQ NIKQIGTFAS VEQFWRFYSH MVRPGDLTGH SDFHLFKEGI 130 140 150 160 170 180 KPMWEDDANK NGGKWIIRLR KGLASRCWEN LILAMLGEQF MVGEEICGAV VSVRFQEDII 190 200 210 220 230 240 SIWNKTASDQ ATTARIRDTL RRVLNLPPNT IMEYKTHTDS IKMPGRLGPQ RLLFQNLWKP RLNVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein." Rom E., Kim H.C., Gingras A.-C., Marcotrigiano J., Favre D., Olsen H., Burley S.K., Sonenberg N. J. Biol. Chem. 273:13104-13109(1998) [PubMed: 9582349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, 3D-STRUCTURE MODELING, MUTAGENESIS. Tissue: Follicular cell.
[2]	"Characterization of mammalian eIF4E-family members." Joshi B., Cameron A., Jagus R. Eur. J. Biochem. 271:2189-2203(2004) [PubMed: 15153109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EIF4EBP1; EIF4EBP2 AND EIF4EBP3. Tissue: Mammary gland.
[3]	"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed: 9653160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle, Urinary bladder and Uterus.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF047695 mRNA. Translation: AAC18565.1.
AF068117 mRNA. Translation: AAC19374.1.
AF038957 mRNA. Translation: AAC39871.1.
BC005392 mRNA. Translation: AAH05392.1.
BC005874 mRNA. Translation: AAH05874.1.
BC021226 mRNA. Translation: AAH21226.1.
BC021690 mRNA. Translation: AAH21690.1.

IPI

IPI00744211.

RefSeq

NP_004837.1.

UniGene

Hs.292026

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JGB	X-ray	1.70	A	45-234	[»]
2JGC	X-ray	2.40	A	45-234	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O60573. 7 interactions.

Proteomic databases

PRIDE

O60573.

Genome annotation databases

Ensembl

ENSG00000135930. Homo sapiens. [Contig view]

GeneID

9470.

KEGG

hsa:9470.

Organism-specific databases

GeneCards

GC02P233123.

H-InvDB

HIX0002926.
HIX0059993.

HGNC

HGNC:3293. EIF4E2.

HPA

HPA019253.

MIM

605895. gene.

PharmGKB

PA27720.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

O60573.

OMA

O60573. LWKPRLN.

Gene expression databases

ArrayExpress

O60573.

Bgee

O60573.

CleanEx

HS_EIF4E2.

GermOnline

ENSG00000135930. Homo sapiens.

Family and domain databases

InterPro

IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]

Gene3D

G3DSA:3.30.760.10. TIF_eIF_4E. 1 hit.

PANTHER

PTHR11960. TIF_eIF_4E. 1 hit.

Pfam

PF01652. IF4E. 1 hit.
[Graphical view]

ProDom

PD003697. TIF_eIF_4E. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00813. IF4E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

35490.

SOURCE

Search...

Entry information

Entry name

IF4E2_HUMAN

Accession

Primary (citable) accession number: O60573
Secondary accession number(s): O75349

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)