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Protein

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene

BUB1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Kinase activity stimulated by CENPE.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei795 – 7951ATPBy similarity
Active sitei882 – 8821Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi772 – 7809ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
ReactomeiR-HSA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-HSA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiO60566.
SIGNORiO60566.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC:2.7.11.1)
Alternative name(s):
MAD3/BUB1-related protein kinase
Short name:
hBUBR1
Mitotic checkpoint kinase MAD3L
Protein SSK1
Gene namesi
Name:BUB1B
Synonyms:BUBR1, MAD3L, SSK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:1149. BUB1B.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: ProtInc
  • condensed chromosome kinetochore Source: UniProtKB
  • condensed chromosome outer kinetochore Source: UniProtKB
  • condensed nuclear chromosome kinetochore Source: Ensembl
  • cytoplasm Source: LIFEdb
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • microtubule organizing center Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: BHF-UCL
  • spindle midzone Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in BUB1B are associated with tumor formation.

Premature chromatid separation trait (PCS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionConsists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
See also OMIM:176430
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti36 – 361R → Q in PCS. 1 Publication
Corresponds to variant rs534297115 [ dbSNP | Ensembl ].
VAR_028921
Mosaic variegated aneuploidy syndrome 1 (MVA1)1 Publication
The disease is caused by mutations affecting the gene represented in this entry. MVA1 is caused by biallelic mutations in the BUB1B gene.
Disease descriptionA severe developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases.
See also OMIM:257300
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti550 – 5501R → Q in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989187 [ dbSNP | Ensembl ].
VAR_028923
Natural varianti814 – 8141R → H in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989182 [ dbSNP | Ensembl ].
VAR_028924
Natural varianti844 – 8441L → F in MVA1; associated with H-921; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989181 [ dbSNP | Ensembl ].
VAR_028925
Natural varianti909 – 9091I → T in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989184 [ dbSNP | Ensembl ].
VAR_028926
Natural varianti921 – 9211Q → H in MVA1; associated with F-844; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989183 [ dbSNP | Ensembl ].
VAR_028927
Natural varianti1012 – 10121L → P in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989185 [ dbSNP | Ensembl ].
VAR_028928

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591A → W: Loss of interaction with CASC5. 1 Publication
Mutagenesisi175 – 1751F → A: Loss of interaction with CASC5. 1 Publication
Mutagenesisi579 – 5791D → E: Abolishes the cleavage by caspase-3. 1 Publication
Mutagenesisi610 – 6101D → E: Abolishes the cleavage by caspase-3. 1 Publication
Mutagenesisi620 – 6201T → A: Induces chromosome congression defects and mitotic delay. 1 Publication
Mutagenesisi795 – 7951K → A: Does not abolish the capacity to inhibit APC/CDC20. 2 Publications
Mutagenesisi795 – 7951K → R: Inhibits kinase activity. 2 Publications

Keywords - Diseasei

Disease mutation, Tumor suppressor

Organism-specific databases

MalaCardsiBUB1B.
MIMi176430. phenotype.
257300. phenotype.
Orphaneti1052. Mosaic variegated aneuploidy syndrome.
PharmGKBiPA82.

Polymorphism and mutation databases

BioMutaiBUB1B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050Mitotic checkpoint serine/threonine-protein kinase BUB1 betaPRO_0000085673Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei250 – 2501N6-acetyllysine; by PCAF1 Publication
Modified residuei367 – 3671PhosphoserineCombined sources
Modified residuei435 – 4351PhosphoserineCombined sources1 Publication
Modified residuei543 – 5431PhosphoserineCombined sources1 Publication
Modified residuei665 – 6651PhosphoserineCombined sources
Modified residuei670 – 6701PhosphoserineCombined sources1 Publication
Modified residuei676 – 6761Phosphoserine; by PLK11 Publication
Modified residuei697 – 6971PhosphoserineCombined sources
Modified residuei792 – 7921Phosphothreonine; by PLK11 Publication
Modified residuei1008 – 10081Phosphothreonine; by PLK11 Publication
Modified residuei1042 – 10421PhosphothreonineCombined sources
Modified residuei1043 – 10431Phosphoserine1 Publication

Post-translational modificationi

Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.1 Publication
Ubiquitinated. Degraded by the proteasome.1 Publication
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.1 Publication
Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei579 – 5802Cleavage; by caspase-3
Sitei610 – 6112Cleavage; by caspase-3

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO60566.
MaxQBiO60566.
PaxDbiO60566.
PeptideAtlasiO60566.
PRIDEiO60566.

PTM databases

iPTMnetiO60566.
PhosphoSiteiO60566.

Expressioni

Tissue specificityi

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.1 Publication

Inductioni

Induced during mitosis.3 Publications

Gene expression databases

BgeeiENSG00000156970.
CleanExiHS_BUB1B.
ExpressionAtlasiO60566. baseline and differential.
GenevisibleiO60566. HS.

Organism-specific databases

HPAiHPA008419.

Interactioni

Subunit structurei

Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5.11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB3O436845EBI-1001438,EBI-1050987
CDC20Q1283416EBI-1001438,EBI-367462
CENPEQ022244EBI-1001438,EBI-1375040
CrebbpP454813EBI-1001438,EBI-296306From a different organism.
KAT2BQ9283114EBI-1001438,EBI-477430
SIRT2Q8IXJ63EBI-1001438,EBI-477232
UBCP0CG483EBI-1001438,EBI-3390054

Protein-protein interaction databases

BioGridi107166. 95 interactions.
DIPiDIP-24203N.
IntActiO60566. 68 interactions.
MINTiMINT-2796866.
STRINGi9606.ENSP00000287598.

Structurei

Secondary structure

1
1050
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni26 – 283Combined sources
Helixi60 – 656Combined sources
Helixi75 – 8814Combined sources
Helixi94 – 963Combined sources
Helixi98 – 10811Combined sources
Turni109 – 1113Combined sources
Helixi113 – 1153Combined sources
Helixi119 – 13113Combined sources
Helixi135 – 14410Combined sources
Helixi152 – 16413Combined sources
Helixi168 – 18013Combined sources
Helixi186 – 21833Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WVIX-ray1.80A57-220[»]
3SI5X-ray2.20A/B57-220[»]
4GGDX-ray2.44C/D20-42[»]
ProteinModelPortaliO60566.
SMRiO60566. Positions 57-203.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60566.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 226165BUB1 N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini766 – 1050285Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 18534Necessary for interaction with CASC5Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi111 – 1188Nuclear localization signalSequence analysis
Motifi224 – 2329D-box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi209 – 2157Poly-Glu

Domaini

The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.Curated
The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

Sequence similaritiesi

Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.Curated

Phylogenomic databases

eggNOGiENOG410IQA2. Eukaryota.
ENOG410XUW7. LUCA.
GeneTreeiENSGT00520000055622.
HOVERGENiHBG050748.
InParanoidiO60566.
KOiK06637.
OMAiATHSSGF.
OrthoDBiEOG091G01MC.
PhylomeDBiO60566.
TreeFamiTF105456.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3/Bub1_I.
IPR000719. Prot_kinase_dom.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60566-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVKKEGGA LSEAMSLEGD EWELSKENVQ PLRQGRIMST LQGALAQESA
60 70 80 90 100
CNNTLQQQKR AFEYEIRFYT GNDPLDVWDR YISWTEQNYP QGGKESNMST
110 120 130 140 150
LLERAVEALQ GEKRYYSDPR FLNLWLKLGR LCNEPLDMYS YLHNQGIGVS
160 170 180 190 200
LAQFYISWAE EYEARENFRK ADAIFQEGIQ QKAEPLERLQ SQHRQFQARV
210 220 230 240 250
SRQTLLALEK EEEEEVFESS VPQRSTLAEL KSKGKKTARA PIIRVGGALK
260 270 280 290 300
APSQNRGLQN PFPQQMQNNS RITVFDENAD EASTAELSKP TVQPWIAPPM
310 320 330 340 350
PRAKENELQA GPWNTGRSLE HRPRGNTASL IAVPAVLPSF TPYVEETARQ
360 370 380 390 400
PVMTPCKIEP SINHILSTRK PGKEEGDPLQ RVQSHQQASE EKKEKMMYCK
410 420 430 440 450
EKIYAGVGEF SFEEIRAEVF RKKLKEQREA ELLTSAEKRA EMQKQIEEME
460 470 480 490 500
KKLKEIQTTQ QERTGDQQEE TMPTKETTKL QIASESQKIP GMTLSSSVCQ
510 520 530 540 550
VNCCARETSL AENIWQEQPH SKGPSVPFSI FDEFLLSEKK NKSPPADPPR
560 570 580 590 600
VLAQRRPLAV LKTSESITSN EDVSPDVCDE FTGIEPLSED AIITGFRNVT
610 620 630 640 650
ICPNPEDTCD FARAARFVST PFHEIMSLKD LPSDPERLLP EEDLDVKTSE
660 670 680 690 700
DQQTACGTIY SQTLSIKKLS PIIEDSREAT HSSGFSGSSA SVASTSSIKC
710 720 730 740 750
LQIPEKLELT NETSENPTQS PWCSQYRRQL LKSLPELSAS AELCIEDRPM
760 770 780 790 800
PKLEIEKEIE LGNEDYCIKR EYLICEDYKL FWVAPRNSAE LTVIKVSSQP
810 820 830 840 850
VPWDFYINLK LKERLNEDFD HFCSCYQYQD GCIVWHQYIN CFTLQDLLQH
860 870 880 890 900
SEYITHEITV LIIYNLLTIV EMLHKAEIVH GDLSPRCLIL RNRIHDPYDC
910 920 930 940 950
NKNNQALKIV DFSYSVDLRV QLDVFTLSGF RTVQILEGQK ILANCSSPYQ
960 970 980 990 1000
VDLFGIADLA HLLLFKEHLQ VFWDGSFWKL SQNISELKDG ELWNKFFVRI
1010 1020 1030 1040 1050
LNANDEATVS VLGELAAEMN GVFDTTFQSH LNKALWKVGK LTSPGALLFQ
Length:1,050
Mass (Da):119,545
Last modified:April 3, 2007 - v3
Checksum:iF7871103A56E6B46
GO
Isoform 2 (identifier: O60566-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-166: Missing.
     522-522: K → KVSLSL
     608-675: Missing.

Note: No experimental confirmation available.
Show »
Length:933
Mass (Da):105,890
Checksum:i81D1307E360D7B67
GO
Isoform 3 (identifier: O60566-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     80-80: R → RWVFLFHKDNRNINR

Note: No experimental confirmation available.
Show »
Length:1,064
Mass (Da):121,386
Checksum:iFB6C9F104164FBEB
GO

Sequence cautioni

The sequence BAD92019 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti248 – 2492AL → VF in AAC23736 (PubMed:9618306).Curated
Sequence conflicti283 – 2831S → P in BAG35587 (PubMed:14702039).Curated
Sequence conflicti788 – 7881S → F in AAC06260 (PubMed:9660858).Curated
Sequence conflicti1018 – 10181E → K in AAC33435 (PubMed:9763420).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti15 – 151M → T in a colorectal cancer cell line. 1 Publication
VAR_008852
Natural varianti36 – 361R → Q in PCS. 1 Publication
Corresponds to variant rs534297115 [ dbSNP | Ensembl ].
VAR_028921
Natural varianti40 – 401T → M.1 Publication
Corresponds to variant rs56079734 [ dbSNP | Ensembl ].
VAR_040402
Natural varianti349 – 3491R → Q.9 Publications
Corresponds to variant rs1801376 [ dbSNP | Ensembl ].
VAR_008853
Natural varianti378 – 3781P → S.1 Publication
Corresponds to variant rs17851677 [ dbSNP | Ensembl ].
VAR_054549
Natural varianti390 – 3901E → D.1 Publication
Corresponds to variant rs1017842 [ dbSNP | Ensembl ].
VAR_028922
Natural varianti550 – 5501R → Q in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989187 [ dbSNP | Ensembl ].
VAR_028923
Natural varianti618 – 6181V → A in colorectal cancer. 2 Publications
Corresponds to variant rs1801528 [ dbSNP | Ensembl ].
VAR_008854
Natural varianti814 – 8141R → H in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989182 [ dbSNP | Ensembl ].
VAR_028924
Natural varianti844 – 8441L → F in MVA1; associated with H-921; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989181 [ dbSNP | Ensembl ].
VAR_028925
Natural varianti909 – 9091I → T in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989184 [ dbSNP | Ensembl ].
VAR_028926
Natural varianti921 – 9211Q → H in MVA1; associated with F-844; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989183 [ dbSNP | Ensembl ].
VAR_028927
Natural varianti1012 – 10121L → P in MVA1; heterozygous compound with nonsense mutation. 1 Publication
Corresponds to variant rs28989185 [ dbSNP | Ensembl ].
VAR_028928

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei80 – 801R → RWVFLFHKDNRNINR in isoform 3. 1 PublicationVSP_036473
Alternative sequencei113 – 16654Missing in isoform 2. 1 PublicationVSP_036474Add
BLAST
Alternative sequencei522 – 5221K → KVSLSL in isoform 2. 1 PublicationVSP_036475
Alternative sequencei608 – 67568Missing in isoform 2. 1 PublicationVSP_036476Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053306 mRNA. Translation: AAC06260.1.
AF046918 mRNA. Translation: AAC33435.1.
AF046079 mRNA. Translation: AAC12730.2.
AF107297 mRNA. Translation: AAD11941.1.
AF035933 mRNA. Translation: AAC23736.1.
AF068760 mRNA. Translation: AAC19118.1.
AF310214
, AF310192, AF310193, AF310194, AF310195, AF310196, AF310197, AF310198, AF310199, AF310200, AF310201, AF310202, AF310203, AF310204, AF310205, AF310206, AF310207, AF310208, AF310209, AF310210, AF310211, AF310212, AF310213 Genomic DNA. Translation: AAL10712.1.
AK296795 mRNA. Translation: BAG59371.1.
AK296984 mRNA. Translation: BAG59525.1.
AK312709 mRNA. Translation: BAG35587.1.
AB208782 mRNA. Translation: BAD92019.1. Different initiation.
BC018739 mRNA. Translation: AAH18739.1.
CCDSiCCDS10053.1. [O60566-1]
PIRiJW0092.
RefSeqiNP_001202.4. NM_001211.5.
UniGeneiHs.513645.

Genome annotation databases

EnsembliENST00000287598; ENSP00000287598; ENSG00000156970. [O60566-1]
ENST00000412359; ENSP00000398470; ENSG00000156970. [O60566-3]
GeneIDi701.
KEGGihsa:701.
UCSCiuc001zkx.5. human. [O60566-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053306 mRNA. Translation: AAC06260.1.
AF046918 mRNA. Translation: AAC33435.1.
AF046079 mRNA. Translation: AAC12730.2.
AF107297 mRNA. Translation: AAD11941.1.
AF035933 mRNA. Translation: AAC23736.1.
AF068760 mRNA. Translation: AAC19118.1.
AF310214
, AF310192, AF310193, AF310194, AF310195, AF310196, AF310197, AF310198, AF310199, AF310200, AF310201, AF310202, AF310203, AF310204, AF310205, AF310206, AF310207, AF310208, AF310209, AF310210, AF310211, AF310212, AF310213 Genomic DNA. Translation: AAL10712.1.
AK296795 mRNA. Translation: BAG59371.1.
AK296984 mRNA. Translation: BAG59525.1.
AK312709 mRNA. Translation: BAG35587.1.
AB208782 mRNA. Translation: BAD92019.1. Different initiation.
BC018739 mRNA. Translation: AAH18739.1.
CCDSiCCDS10053.1. [O60566-1]
PIRiJW0092.
RefSeqiNP_001202.4. NM_001211.5.
UniGeneiHs.513645.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WVIX-ray1.80A57-220[»]
3SI5X-ray2.20A/B57-220[»]
4GGDX-ray2.44C/D20-42[»]
ProteinModelPortaliO60566.
SMRiO60566. Positions 57-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107166. 95 interactions.
DIPiDIP-24203N.
IntActiO60566. 68 interactions.
MINTiMINT-2796866.
STRINGi9606.ENSP00000287598.

PTM databases

iPTMnetiO60566.
PhosphoSiteiO60566.

Polymorphism and mutation databases

BioMutaiBUB1B.

Proteomic databases

EPDiO60566.
MaxQBiO60566.
PaxDbiO60566.
PeptideAtlasiO60566.
PRIDEiO60566.

Protocols and materials databases

DNASUi701.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287598; ENSP00000287598; ENSG00000156970. [O60566-1]
ENST00000412359; ENSP00000398470; ENSG00000156970. [O60566-3]
GeneIDi701.
KEGGihsa:701.
UCSCiuc001zkx.5. human. [O60566-1]

Organism-specific databases

CTDi701.
GeneCardsiBUB1B.
H-InvDBHIX0012121.
HGNCiHGNC:1149. BUB1B.
HPAiHPA008419.
MalaCardsiBUB1B.
MIMi176430. phenotype.
257300. phenotype.
602860. gene.
neXtProtiNX_O60566.
Orphaneti1052. Mosaic variegated aneuploidy syndrome.
PharmGKBiPA82.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQA2. Eukaryota.
ENOG410XUW7. LUCA.
GeneTreeiENSGT00520000055622.
HOVERGENiHBG050748.
InParanoidiO60566.
KOiK06637.
OMAiATHSSGF.
OrthoDBiEOG091G01MC.
PhylomeDBiO60566.
TreeFamiTF105456.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
ReactomeiR-HSA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-HSA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.
SignaLinkiO60566.
SIGNORiO60566.

Miscellaneous databases

EvolutionaryTraceiO60566.
GeneWikiiBUB1B.
GenomeRNAii701.
PROiO60566.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156970.
CleanExiHS_BUB1B.
ExpressionAtlasiO60566. baseline and differential.
GenevisibleiO60566. HS.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3/Bub1_I.
IPR000719. Prot_kinase_dom.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUB1B_HUMAN
AccessioniPrimary (citable) accession number: O60566
Secondary accession number(s): B2R6U0
, B4DL09, B4DLG3, O60501, O60627, O60758, O75389, Q59HH6, Q8WV50, Q96KM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 3, 2007
Last modified: September 7, 2016
This is version 171 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.