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O60547 (GMDS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-mannose 4,6 dehydratase

EC=4.2.1.47
Alternative name(s):
GDP-D-mannose dehydratase
Short name=GMD
Gene names
Name:GMDS
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose.

Catalytic activity

GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.

Cofactor

NADP.

Pathway

Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.

Sequence similarities

Belongs to the GDP-mannose 4,6-dehydratase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 372371GDP-mannose 4,6 dehydratase
PRO_0000201705

Sites

Active site1551 By similarity
Active site1571Nucleophile By similarity
Active site1791Nucleophile By similarity
Binding site1831NADP By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.6

Experimental info

Sequence conflict341Q → M in AAC24501. Ref.5
Sequence conflict1561S → N in CAG46745. Ref.2

Secondary structure

............................................................ 372
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O60547 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B1BAC441736D4C2B

FASTA37241,950
        10         20         30         40         50         60 
MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF 

        70         80         90        100        110        120 
NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL 

       130        140        150        160        170        180 
AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG 

       190        200        210        220        230        240 
AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL 

       250        260        270        280        290        300 
GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG 

       310        320        330        340        350        360 
KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH 

       370 
ADVELMRTNP NA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of human GDP-mannose 4,6-dehydratase and reconstitution of GDP-fucose biosynthesis in vitro."
Sullivan F.X., Kumar R., Kriz R., Stahl M., Xu G.-Y., Rouse J., Chang X.J., Boodhoo A., Potvin B., Cumming D.A.
J. Biol. Chem. 273:8193-8202(1998) [PubMed: 9525924] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed: 14574404] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[5]"Molecular cloning and expression of GDP-D-mannose-4,6-dehydratase, a key enzyme for fucose metabolism defective in Lec13 cells."
Ohyama C., Smith P.L., Angata K., Fukuda M.N., Lowe J.B., Fukuda M.
J. Biol. Chem. 273:14582-14587(1998) [PubMed: 9603974] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-372.
Tissue: Brain.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF042377 mRNA. Translation: AAC13553.1.
CR541929 mRNA. Translation: CAG46727.1.
CR541947 mRNA. Translation: CAG46745.1.
AL033517 expand/collapse EMBL AC list , AL034344, AL035693, AL137179, AL158139 Genomic DNA. Translation: CAI20018.1.
AL035693 expand/collapse EMBL AC list , AL033517, AL034344, AL137179, AL158139 Genomic DNA. Translation: CAB65990.2.
AL137179 expand/collapse EMBL AC list , AL033517, AL034344, AL035693, AL158139 Genomic DNA. Translation: CAI16872.1.
AL158139 expand/collapse EMBL AC list , AL033517, AL034344, AL035693, AL137179 Genomic DNA. Translation: CAI22880.1.
AL034344 expand/collapse EMBL AC list , AL033517, AL035693, AL137179, AL158139 Genomic DNA. Translation: CAI19400.1.
BC000117 mRNA. Translation: AAH00117.1.
AF040260 mRNA. Translation: AAC24501.1.
IPIIPI00030207.
RefSeqNP_001491.1. NM_001500.2.
UniGeneHs.144496.
Hs.660919.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1T2AX-ray1.84A/B/C/D23-372[»]
ProteinModelPortalO60547.
SMRO60547. Positions 23-369.
ModBaseSearch...

Protein-protein interaction databases

IntActO60547. 2 interactions.
STRINGO60547.

PTM databases

PhosphoSiteO60547.

Proteomic databases

PeptideAtlasO60547.
PRIDEO60547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380815; ENSP00000370194; ENSG00000112699.
GeneID2762.
KEGGhsa:2762.
UCSCuc003mtq.1. human.

Organism-specific databases

CTD2762.
GeneCardsGC06M001624.
H-InvDBHIX0022484.
HGNCHGNC:4369. GMDS.
HPAHPA031528.
MIM602884. gene.
neXtProtNX_O60547.
PharmGKBPA28754.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG17652.
HOGENOMHBG755066.
HOVERGENHBG000727.
InParanoidO60547.
OMATQRVDHI.
OrthoDBEOG4FXR7M.
PhylomeDBO60547.

Gene expression databases

ArrayExpressO60547.
BgeeO60547.
CleanExHS_GMDS.
GenevestigatorO60547.
GermOnlineENSG00000112699. Homo sapiens.

Family and domain databases

InterProIPR001509. Epimerase_deHydtase.
IPR006368. GDP_Man_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01711.
PANTHERPTHR10366:SF32. GDP_mann_dehyd. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01472. Gmd. 1 hit.
ProtoNetSearch...

Other

NextBio10866.
SOURCESearch...

Entry information

Entry nameGMDS_HUMAN
AccessionPrimary (citable) accession number: O60547
Secondary accession number(s): O75357 expand/collapse secondary AC list , Q5T954, Q6FH09, Q9UGZ3, Q9UJK9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families