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Protein

Eukaryotic translation initiation factor 4E-binding protein 3

Gene

EIF4EBP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation.By similarity

GO - Molecular functioni

  1. translation repressor activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protein synthesis inhibitor

Keywords - Biological processi

Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E-binding protein 3
Short name:
4E-BP3
Short name:
eIF4E-binding protein 3
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3290. EIF4EBP3.

Subcellular locationi

GO - Cellular componenti

  1. eukaryotic translation initiation factor 4F complex Source: UniProtKB
  2. membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401Y → A: Loss of interaction with EIF4E. 1 Publication
Mutagenesisi45 – 451L → A: Loss of interaction with EIF4E. 1 Publication

Organism-specific databases

PharmGKBiPA27718.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100Eukaryotic translation initiation factor 4E-binding protein 3PRO_0000190518Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO60516.
PRIDEiO60516.

PTM databases

PhosphoSiteiO60516.

Expressioni

Tissue specificityi

Expression is highest in skeletal muscle, heart, kidney, and pancreas, whereas there is very little expression in brain and thymus.1 Publication

Gene expression databases

BgeeiO60516.
GenevestigatoriO60516.

Organism-specific databases

HPAiHPA045537.

Interactioni

Subunit structurei

EIF4EBP3 interacts with EIF4E.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF4EP067302EBI-746950,EBI-73440
Eif4e2Q80ZJ32EBI-746950,EBI-934970From a different organism.
RPA2P159273EBI-746950,EBI-621404

Protein-protein interaction databases

BioGridi114190. 8 interactions.
IntActiO60516. 5 interactions.
MINTiMINT-1535204.
STRINGi9606.ENSP00000308472.

Structurei

3D structure databases

ProteinModelPortaliO60516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 467YXXXXLphi motif1 Publication
Motifi96 – 1005TOS motifBy similarity

Sequence similaritiesi

Belongs to the eIF4E-binding protein family.Curated

Phylogenomic databases

eggNOGiNOG78084.
GeneTreeiENSGT00390000013843.
HOGENOMiHOG000231190.
HOVERGENiHBG050425.
InParanoidiO60516.
KOiK18645.
OMAiNQFEMDI.
OrthoDBiEOG7B31Q1.
PhylomeDBiO60516.
TreeFamiTF101530.

Family and domain databases

InterProiIPR008606. EIF4EBP.
[Graphical view]
PfamiPF05456. eIF_4EBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSTSCPIP GGRDQLPDCY STTPGGTLYA TTPGGTRIIY DRKFLLECKN
60 70 80 90 100
SPIARTPPCC LPQIPGVTTP PTAPLSKLEE LKEQETEEEI PDDAQFEMDI
Length:100
Mass (Da):10,873
Last modified:July 31, 1998 - v1
Checksum:iFD29A7F3829E546E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038869 mRNA. Translation: AAC39761.1.
BC010881 mRNA. Translation: AAH10881.1.
BC069293 mRNA. Translation: AAH69293.1.
BC073751 mRNA. Translation: AAH73751.1.
CCDSiCCDS4226.1.
RefSeqiNP_003723.1. NM_003732.2.
UniGeneiHs.594084.

Genome annotation databases

EnsembliENST00000310331; ENSP00000308472; ENSG00000243056.
GeneIDi8637.
KEGGihsa:8637.
UCSCiuc003lfy.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038869 mRNA. Translation: AAC39761.1.
BC010881 mRNA. Translation: AAH10881.1.
BC069293 mRNA. Translation: AAH69293.1.
BC073751 mRNA. Translation: AAH73751.1.
CCDSiCCDS4226.1.
RefSeqiNP_003723.1. NM_003732.2.
UniGeneiHs.594084.

3D structure databases

ProteinModelPortaliO60516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114190. 8 interactions.
IntActiO60516. 5 interactions.
MINTiMINT-1535204.
STRINGi9606.ENSP00000308472.

PTM databases

PhosphoSiteiO60516.

Proteomic databases

PaxDbiO60516.
PRIDEiO60516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310331; ENSP00000308472; ENSG00000243056.
GeneIDi8637.
KEGGihsa:8637.
UCSCiuc003lfy.1. human.

Organism-specific databases

CTDi8637.
GeneCardsiGC05P139928.
HGNCiHGNC:3290. EIF4EBP3.
HPAiHPA045537.
MIMi603483. gene.
neXtProtiNX_O60516.
PharmGKBiPA27718.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG78084.
GeneTreeiENSGT00390000013843.
HOGENOMiHOG000231190.
HOVERGENiHBG050425.
InParanoidiO60516.
KOiK18645.
OMAiNQFEMDI.
OrthoDBiEOG7B31Q1.
PhylomeDBiO60516.
TreeFamiTF101530.

Miscellaneous databases

GeneWikiiEIF4EBP3.
GenomeRNAii8637.
NextBioi32379.
PROiO60516.
SOURCEiSearch...

Gene expression databases

BgeeiO60516.
GenevestigatoriO60516.

Family and domain databases

InterProiIPR008606. EIF4EBP.
[Graphical view]
PfamiPF05456. eIF_4EBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "4E-BP3, a new member of the eukaryotic initiation factor 4E-binding protein family."
    Poulin F., Gingras A.-C., Olsen H., Chevalier S., Sonenberg N.
    J. Biol. Chem. 273:14002-14007(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EIF4E, MUTAGENESIS OF TYR-40 AND LEU-45, TISSUE SPECIFICITY, PHOSPHORYLATION.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph.

Entry informationi

Entry namei4EBP3_HUMAN
AccessioniPrimary (citable) accession number: O60516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2003
Last sequence update: July 31, 1998
Last modified: March 3, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.