Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.1 Publication
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=238 µM for GlcNAc-B-S-pNP1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961ManganeseBy similarity
Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
Binding sitei256 – 2561UDP-alpha-D-galactoseBy similarity
Metal bindingi289 – 2891Manganese; via tele nitrogenBy similarity
Binding sitei301 – 3011N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. galactosyltransferase activity Source: ProtInc
  2. metal ion binding Source: UniProtKB-KW
  3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. cellular protein metabolic process Source: Reactome
  3. glycosaminoglycan metabolic process Source: Reactome
  4. keratan sulfate biosynthetic process Source: Reactome
  5. keratan sulfate metabolic process Source: Reactome
  6. membrane lipid metabolic process Source: ProtInc
  7. post-translational protein modification Source: Reactome
  8. protein N-linked glycosylation via asparagine Source: Reactome
  9. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04504-MONOMER.
ReactomeiREACT_121120. Keratan sulfate biosynthesis.
REACT_25085. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4GALT4
ORF Names:UNQ552/PRO1109
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:927. B4GALT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3826Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini39 – 344306LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25226.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Beta-1,4-galactosyltransferase 4PRO_0000080542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi77 ↔ 118By similarity
Disulfide bondi189 ↔ 208By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO60513.
PaxDbiO60513.
PRIDEiO60513.

PTM databases

PhosphoSiteiO60513.

Expressioni

Tissue specificityi

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Gene expression databases

BgeeiO60513.
CleanExiHS_B4GALT4.
ExpressionAtlasiO60513. baseline and differential.
GenevestigatoriO60513.

Organism-specific databases

HPAiHPA046819.

Interactioni

Protein-protein interaction databases

BioGridi114245. 5 interactions.
STRINGi9606.ENSP00000352144.

Structurei

3D structure databases

ProteinModelPortaliO60513.
SMRiO60513. Positions 77-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1335UDP-alpha-D-galactose bindingBy similarity
Regioni168 – 1703UDP-alpha-D-galactose bindingBy similarity
Regioni195 – 1962UDP-alpha-D-galactose bindingBy similarity
Regioni258 – 2614N-acetyl-D-glucosamine bindingBy similarity
Regioni289 – 2913UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO60513.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG7060R0.
PhylomeDBiO60513.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O60513-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN
60 70 80 90 100
FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE
110 120 130 140 150
VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR
160 170 180 190 200
QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE
210 220 230 240 250
NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS
260 270 280 290 300
NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE
310 320 330 340
RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA
Length:344
Mass (Da):40,041
Last modified:August 1, 1998 - v1
Checksum:i6B7742676FE8A58B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821L → R in AAG50147. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti116 – 1161Q → E.3 Publications
Corresponds to variant rs3764779 [ dbSNP | Ensembl ].
VAR_022697

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038662 mRNA. Translation: AAC39735.1.
AF022367 mRNA. Translation: AAC72493.1.
AB024436 mRNA. Translation: BAA75821.1.
AF020920 mRNA. Translation: AAG50147.1.
AY359008 mRNA. Translation: AAQ89367.1.
CR749555 mRNA. Translation: CAH18352.1.
BC004523 mRNA. Translation: AAH04523.1.
BC062618 mRNA. Translation: AAH62618.1.
CCDSiCCDS2986.1.
RefSeqiNP_003769.1. NM_003778.3.
NP_997708.1. NM_212543.1.
XP_005247912.1. XM_005247855.1.
XP_006713861.1. XM_006713798.1.
XP_006713862.1. XM_006713799.1.
XP_006713863.1. XM_006713800.1.
XP_006713864.1. XM_006713801.1.
UniGeneiHs.13225.

Genome annotation databases

EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
ENST00000393765; ENSP00000377360; ENSG00000121578.
ENST00000483209; ENSP00000420161; ENSG00000121578.
GeneIDi8702.
KEGGihsa:8702.
UCSCiuc003ece.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038662 mRNA. Translation: AAC39735.1.
AF022367 mRNA. Translation: AAC72493.1.
AB024436 mRNA. Translation: BAA75821.1.
AF020920 mRNA. Translation: AAG50147.1.
AY359008 mRNA. Translation: AAQ89367.1.
CR749555 mRNA. Translation: CAH18352.1.
BC004523 mRNA. Translation: AAH04523.1.
BC062618 mRNA. Translation: AAH62618.1.
CCDSiCCDS2986.1.
RefSeqiNP_003769.1. NM_003778.3.
NP_997708.1. NM_212543.1.
XP_005247912.1. XM_005247855.1.
XP_006713861.1. XM_006713798.1.
XP_006713862.1. XM_006713799.1.
XP_006713863.1. XM_006713800.1.
XP_006713864.1. XM_006713801.1.
UniGeneiHs.13225.

3D structure databases

ProteinModelPortaliO60513.
SMRiO60513. Positions 77-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114245. 5 interactions.
STRINGi9606.ENSP00000352144.

Chemistry

DrugBankiDB00141. N-Acetyl-D-glucosamine.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiO60513.

Proteomic databases

MaxQBiO60513.
PaxDbiO60513.
PRIDEiO60513.

Protocols and materials databases

DNASUi8702.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
ENST00000393765; ENSP00000377360; ENSG00000121578.
ENST00000483209; ENSP00000420161; ENSG00000121578.
GeneIDi8702.
KEGGihsa:8702.
UCSCiuc003ece.1. human.

Organism-specific databases

CTDi8702.
GeneCardsiGC03M118930.
HGNCiHGNC:927. B4GALT4.
HPAiHPA046819.
MIMi604015. gene.
neXtProtiNX_O60513.
PharmGKBiPA25226.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO60513.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG7060R0.
PhylomeDBiO60513.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:HS04504-MONOMER.
ReactomeiREACT_121120. Keratan sulfate biosynthesis.
REACT_25085. N-Glycan antennae elongation.

Miscellaneous databases

GeneWikiiB4GALT4.
GenomeRNAii8702.
NextBioi32625.
PROiO60513.
SOURCEiSearch...

Gene expression databases

BgeeiO60513.
CleanExiHS_B4GALT4.
ExpressionAtlasiO60513. baseline and differential.
GenevestigatoriO60513.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis."
    Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H.
    J. Biol. Chem. 273:29331-29340(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
  3. "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
    Guo S., Sato T., Shirane K., Furukawa K.
    Glycobiology 11:813-820(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family."
    Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S.
    Sci. China, Ser. C, Life Sci. 42:337-345(1999)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
    Tissue: Fetal kidney.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
    Tissue: Colon and Muscle.
  8. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
    Amado M., Almeida R., Schwientek T., Clausen H.
    Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiB4GT4_HUMAN
AccessioniPrimary (citable) accession number: O60513
Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.