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O60513

- B4GT4_HUMAN

UniProt

O60513 - B4GT4_HUMAN

Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.1 Publication
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

    Cofactori

    Manganese.By similarity

    Kineticsi

    1. KM=238 µM for GlcNAc-B-S-pNP1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi196 – 1961ManganeseBy similarity
    Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
    Binding sitei256 – 2561UDP-alpha-D-galactoseBy similarity
    Metal bindingi289 – 2891Manganese; via tele nitrogenBy similarity
    Binding sitei301 – 3011N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. galactosyltransferase activity Source: ProtInc
    2. metal ion binding Source: UniProtKB-KW
    3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. cellular protein metabolic process Source: Reactome
    3. glycosaminoglycan metabolic process Source: Reactome
    4. keratan sulfate biosynthetic process Source: Reactome
    5. keratan sulfate metabolic process Source: Reactome
    6. membrane lipid metabolic process Source: ProtInc
    7. post-translational protein modification Source: Reactome
    8. protein N-linked glycosylation via asparagine Source: Reactome
    9. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS04504-MONOMER.
    ReactomeiREACT_121120. Keratan sulfate biosynthesis.
    REACT_25085. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 4
    Short name:
    Beta4Gal-T4
    Short name:
    b4Gal-T4
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    Including the following 2 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4GALT4
    ORF Names:UNQ552/PRO1109
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:927. B4GALT4.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: Reactome
    3. integral component of membrane Source: ProtInc

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25226.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Beta-1,4-galactosyltransferase 4PRO_0000080542Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi77 ↔ 118By similarity
    Disulfide bondi189 ↔ 208By similarity
    Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO60513.
    PaxDbiO60513.
    PRIDEiO60513.

    PTM databases

    PhosphoSiteiO60513.

    Expressioni

    Tissue specificityi

    High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

    Gene expression databases

    ArrayExpressiO60513.
    BgeeiO60513.
    CleanExiHS_B4GALT4.
    GenevestigatoriO60513.

    Organism-specific databases

    HPAiHPA046819.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000352144.

    Structurei

    3D structure databases

    ProteinModelPortaliO60513.
    SMRiO60513. Positions 77-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini39 – 344306LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3826Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1335UDP-alpha-D-galactose bindingBy similarity
    Regioni168 – 1703UDP-alpha-D-galactose bindingBy similarity
    Regioni195 – 1962UDP-alpha-D-galactose bindingBy similarity
    Regioni258 – 2614N-acetyl-D-glucosamine bindingBy similarity
    Regioni289 – 2913UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO60513.
    KOiK07969.
    OMAiHTRDKGN.
    OrthoDBiEOG7060R0.
    PhylomeDBiO60513.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O60513-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN    50
    FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE 100
    VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR 150
    QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE 200
    NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS 250
    NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE 300
    RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA 344
    Length:344
    Mass (Da):40,041
    Last modified:August 1, 1998 - v1
    Checksum:i6B7742676FE8A58B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1821L → R in AAG50147. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti116 – 1161Q → E.3 Publications
    Corresponds to variant rs3764779 [ dbSNP | Ensembl ].
    VAR_022697

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038662 mRNA. Translation: AAC39735.1.
    AF022367 mRNA. Translation: AAC72493.1.
    AB024436 mRNA. Translation: BAA75821.1.
    AF020920 mRNA. Translation: AAG50147.1.
    AY359008 mRNA. Translation: AAQ89367.1.
    CR749555 mRNA. Translation: CAH18352.1.
    BC004523 mRNA. Translation: AAH04523.1.
    BC062618 mRNA. Translation: AAH62618.1.
    CCDSiCCDS2986.1.
    RefSeqiNP_003769.1. NM_003778.3.
    NP_997708.1. NM_212543.1.
    XP_005247912.1. XM_005247855.1.
    XP_006713861.1. XM_006713798.1.
    XP_006713862.1. XM_006713799.1.
    XP_006713863.1. XM_006713800.1.
    XP_006713864.1. XM_006713801.1.
    UniGeneiHs.13225.

    Genome annotation databases

    EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
    ENST00000393765; ENSP00000377360; ENSG00000121578.
    ENST00000483209; ENSP00000420161; ENSG00000121578.
    GeneIDi8702.
    KEGGihsa:8702.
    UCSCiuc003ece.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Beta-1,4-galactosyltransferase 4

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038662 mRNA. Translation: AAC39735.1 .
    AF022367 mRNA. Translation: AAC72493.1 .
    AB024436 mRNA. Translation: BAA75821.1 .
    AF020920 mRNA. Translation: AAG50147.1 .
    AY359008 mRNA. Translation: AAQ89367.1 .
    CR749555 mRNA. Translation: CAH18352.1 .
    BC004523 mRNA. Translation: AAH04523.1 .
    BC062618 mRNA. Translation: AAH62618.1 .
    CCDSi CCDS2986.1.
    RefSeqi NP_003769.1. NM_003778.3.
    NP_997708.1. NM_212543.1.
    XP_005247912.1. XM_005247855.1.
    XP_006713861.1. XM_006713798.1.
    XP_006713862.1. XM_006713799.1.
    XP_006713863.1. XM_006713800.1.
    XP_006713864.1. XM_006713801.1.
    UniGenei Hs.13225.

    3D structure databases

    ProteinModelPortali O60513.
    SMRi O60513. Positions 77-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000352144.

    Chemistry

    DrugBanki DB00141. N-Acetyl-D-glucosamine.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei O60513.

    Proteomic databases

    MaxQBi O60513.
    PaxDbi O60513.
    PRIDEi O60513.

    Protocols and materials databases

    DNASUi 8702.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359213 ; ENSP00000352144 ; ENSG00000121578 .
    ENST00000393765 ; ENSP00000377360 ; ENSG00000121578 .
    ENST00000483209 ; ENSP00000420161 ; ENSG00000121578 .
    GeneIDi 8702.
    KEGGi hsa:8702.
    UCSCi uc003ece.1. human.

    Organism-specific databases

    CTDi 8702.
    GeneCardsi GC03M118930.
    HGNCi HGNC:927. B4GALT4.
    HPAi HPA046819.
    MIMi 604015. gene.
    neXtProti NX_O60513.
    PharmGKBi PA25226.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG327897.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi O60513.
    KOi K07969.
    OMAi HTRDKGN.
    OrthoDBi EOG7060R0.
    PhylomeDBi O60513.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:HS04504-MONOMER.
    Reactomei REACT_121120. Keratan sulfate biosynthesis.
    REACT_25085. N-Glycan antennae elongation.

    Miscellaneous databases

    GeneWikii B4GALT4.
    GenomeRNAii 8702.
    NextBioi 32625.
    PROi O60513.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60513.
    Bgeei O60513.
    CleanExi HS_B4GALT4.
    Genevestigatori O60513.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
      Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
      Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis."
      Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H.
      J. Biol. Chem. 273:29331-29340(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    3. "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
      Guo S., Sato T., Shirane K., Furukawa K.
      Glycobiology 11:813-820(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family."
      Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S.
      Sci. China, Ser. C, Life Sci. 42:337-345(1999)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
      Tissue: Fetal kidney.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
      Tissue: Colon and Muscle.
    8. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
      Amado M., Almeida R., Schwientek T., Clausen H.
      Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiB4GT4_HUMAN
    AccessioniPrimary (citable) accession number: O60513
    Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3