Reviewed, UniProtKB/Swiss-Prot O60513 (B4GT4_HUMAN)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 4
      Short name=Beta-1,4-GalTase 4
      Short name=Beta4Gal-T4
      Short name=b4Gal-T4
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
Including the following 2 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:	B4GALT4
ORF Names:	UNQ552/PRO1109

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Catalytic activity	UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.
Tissue specificity	High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.
Sequence similarities	Belongs to the glycosyltransferase 7 family.
Biophysicochemical properties	Kinetic parameters: KM=238 µM for GlcNAc-B-S-pNP

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro membrane lipid metabolic process Ref.2 Traceable author statement. Source: ProtInc
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Beta-1,4-galactosyltransferase 4		PRO_0000080542
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 38	26	Signal-anchor for type II membrane protein Potential
	Topological domain	39 – 344	306	Lumenal Potential
Sites
	Metal binding	196	1	Manganese By similarity
	Metal binding	289	1	Manganese By similarity
Amino acid modifications
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Disulfide bond	77 ↔ 118		By similarity
	Disulfide bond	189 ↔ 208		By similarity
Natural variations
	Natural variant	116	1	Q → E: dbSNP rs3764779. Ref.4 Ref.6 Ref.7		VAR_022697
Experimental info
	Sequence conflict	182	1	L → R in AAG50147. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O60513-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6B7742676FE8A58B	FASTA	344	40,041
	10 20 30 40 50 60 MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN FHKTLILGKG 70 80 90 100 110 120 KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE VQAENPKVSR GRYRPQECKA 130 140 150 160 170 180 LQRVAILVPH RNREKHLMYL LEHLHPFLQR QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE 190 200 210 220 230 240 ALKEENWDCF IFHDVDLVPE NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR 250 260 270 280 290 300 EQFFKVNGFS NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE 310 320 330 340 RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expanding beta 4-galactosyltransferase gene family: messages from the databanks." Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L. Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis." Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H. J. Biol. Chem. 273:29331-29340(1998) [PubMed: 9792633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells." Guo S., Sato T., Shirane K., Furukawa K. Glycobiology 11:813-820(2001) [PubMed: 11588157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family." Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S. Sci. China, Ser. C, Life Sci. 42:337-345(1999) Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116. Tissue: Fetal kidney.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116. Tissue: Colon and Muscle.
[8]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 4

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038662 mRNA. Translation: AAC39735.1. AF022367 mRNA. Translation: AAC72493.1. AB024436 mRNA. Translation: BAA75821.1. AF020920 mRNA. Translation: AAG50147.1. AY359008 mRNA. Translation: AAQ89367.1. CR749555 mRNA. Translation: CAH18352.1. BC004523 mRNA. Translation: AAH04523.1. BC062618 mRNA. Translation: AAH62618.1.
IPI	IPI00030128.
RefSeq	NP_003769.1. NP_997708.1.
UniGene	Hs.13225
3D structure databases
HSSP	HSSP built from PDB template 1O0R based on UniProtKB P08037.
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	O60513.
Genome annotation databases
Ensembl	ENSG00000121578. Homo sapiens. [Contig view]
GeneID	8702.
KEGG	hsa:8702.
UCSC	uc003ecg.1. human.
Organism-specific databases
GeneCards	GC03M120413.
H-InvDB	HIX0003582.
HGNC	HGNC:927. B4GALT4.
MIM	604015. gene.
PharmGKB	PA25226.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60513.
HOVERGEN	O60513.
OMA	O60513. VWRTDGL.
Enzyme and pathway databases
BRENDA	2.4.1.90. 247.
Gene expression databases
ArrayExpress	O60513.
Bgee	O60513.
CleanEx	HS_B4GALT4.
GermOnline	ENSG00000121578. Homo sapiens.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00141. N-Acetyl-D-glucosamine.
NextBio	32625.
SOURCE	Search...

Entry information

Entry name

B4GT4_HUMAN

Accession

Primary (citable) accession number: O60513
Secondary accession number(s): Q68D68, Q9BSW3, Q9C078

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	August 1, 1998
Last modified:	July 7, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)