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O60513 (B4GT4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 4

Short name=Beta-1,4-GalTase 4
Short name=Beta4Gal-T4
Short name=b4Gal-T4
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Including the following 2 domains:

  1. N-acetyllactosamine synthase
    EC=2.4.1.90
    Alternative name(s):
    Nal synthase
  2. Lactotriaosylceramide beta-1,4-galactosyltransferase
    EC=2.4.1.275
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene names
Name:B4GALT4
ORF Names:UNQ552/PRO1109
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Ref.2

Catalytic activity

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine. Ref.2

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide. Ref.2

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.

Tissue specificity

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Biophysicochemical properties

Kinetic parameters:

KM=238 µM for GlcNAc-B-S-pNP Ref.3

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandManganese
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Traceable author statement. Source: Reactome

cellular protein metabolic process

Traceable author statement. Source: Reactome

glycosaminoglycan metabolic process

Traceable author statement. Source: Reactome

keratan sulfate biosynthetic process

Traceable author statement. Source: Reactome

keratan sulfate metabolic process

Traceable author statement. Source: Reactome

membrane lipid metabolic process

Traceable author statement Ref.2. Source: ProtInc

post-translational protein modification

Traceable author statement. Source: Reactome

protein N-linked glycosylation via asparagine

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentGolgi cisterna membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Golgi membrane

Traceable author statement. Source: Reactome

integral component of membrane

Traceable author statement Ref.2. Source: ProtInc

   Molecular_functionN-acetyllactosamine synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

galactosyltransferase activity

Traceable author statement Ref.2. Source: ProtInc

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080542

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3826Helical; Signal-anchor for type II membrane protein; Potential
Topological domain39 – 344306Lumenal Potential
Region129 – 1335UDP-alpha-D-galactose binding By similarity
Region168 – 1703UDP-alpha-D-galactose binding By similarity
Region195 – 1962UDP-alpha-D-galactose binding By similarity
Region258 – 2614N-acetyl-D-glucosamine binding By similarity
Region289 – 2913UDP-alpha-D-galactose binding By similarity

Sites

Metal binding1961Manganese By similarity
Metal binding2891Manganese; via tele nitrogen By similarity
Binding site2241UDP-alpha-D-galactose By similarity
Binding site2561UDP-alpha-D-galactose By similarity
Binding site3011N-acetyl-D-glucosamine By similarity

Amino acid modifications

Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential
Disulfide bond77 ↔ 118 By similarity
Disulfide bond189 ↔ 208 By similarity

Natural variations

Natural variant1161Q → E. Ref.4 Ref.6 Ref.7
Corresponds to variant rs3764779 [ dbSNP | Ensembl ].
VAR_022697

Experimental info

Sequence conflict1821L → R in AAG50147. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O60513 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 6B7742676FE8A58B

FASTA34440,041
        10         20         30         40         50         60 
MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN FHKTLILGKG 

        70         80         90        100        110        120 
KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE VQAENPKVSR GRYRPQECKA 

       130        140        150        160        170        180 
LQRVAILVPH RNREKHLMYL LEHLHPFLQR QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE 

       190        200        210        220        230        240 
ALKEENWDCF IFHDVDLVPE NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR 

       250        260        270        280        290        300 
EQFFKVNGFS NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE 

       310        320        330        340 
RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA 

« Hide

References

« Hide 'large scale' references
[1]"The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis."
Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H.
J. Biol. Chem. 273:29331-29340(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
[3]"Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
Guo S., Sato T., Shirane K., Furukawa K.
Glycobiology 11:813-820(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
[4]"Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family."
Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S.
Sci. China, Ser. C, Life Sci. 42:337-345(1999)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
Tissue: Fetal kidney.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
Tissue: Colon and Muscle.
[8]"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
Amado M., Almeida R., Schwientek T., Clausen H.
Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 4

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF038662 mRNA. Translation: AAC39735.1.
AF022367 mRNA. Translation: AAC72493.1.
AB024436 mRNA. Translation: BAA75821.1.
AF020920 mRNA. Translation: AAG50147.1.
AY359008 mRNA. Translation: AAQ89367.1.
CR749555 mRNA. Translation: CAH18352.1.
BC004523 mRNA. Translation: AAH04523.1.
BC062618 mRNA. Translation: AAH62618.1.
CCDSCCDS2986.1.
RefSeqNP_003769.1. NM_003778.3.
NP_997708.1. NM_212543.1.
XP_005247912.1. XM_005247855.1.
XP_006713861.1. XM_006713798.1.
XP_006713862.1. XM_006713799.1.
XP_006713863.1. XM_006713800.1.
XP_006713864.1. XM_006713801.1.
UniGeneHs.13225.

3D structure databases

ProteinModelPortalO60513.
SMRO60513. Positions 77-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000352144.

Chemistry

DrugBankDB00141. N-Acetyl-D-glucosamine.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteO60513.

Proteomic databases

MaxQBO60513.
PaxDbO60513.
PRIDEO60513.

Protocols and materials databases

DNASU8702.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359213; ENSP00000352144; ENSG00000121578.
ENST00000393765; ENSP00000377360; ENSG00000121578.
ENST00000483209; ENSP00000420161; ENSG00000121578.
GeneID8702.
KEGGhsa:8702.
UCSCuc003ece.1. human.

Organism-specific databases

CTD8702.
GeneCardsGC03M118930.
HGNCHGNC:927. B4GALT4.
HPAHPA046819.
MIM604015. gene.
neXtProtNX_O60513.
PharmGKBPA25226.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327897.
HOGENOMHOG000231027.
HOVERGENHBG058334.
InParanoidO60513.
KOK07969.
OMAHTRDKGN.
OrthoDBEOG7060R0.
PhylomeDBO60513.
TreeFamTF312834.

Enzyme and pathway databases

BioCycMetaCyc:HS04504-MONOMER.
ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
REACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressO60513.
BgeeO60513.
CleanExHS_B4GALT4.
GenevestigatorO60513.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

GeneWikiB4GALT4.
GenomeRNAi8702.
NextBio32625.
PROO60513.
SOURCESearch...

Entry information

Entry nameB4GT4_HUMAN
AccessionPrimary (citable) accession number: O60513
Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM