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O60513

- B4GT4_HUMAN

UniProt

O60513 - B4GT4_HUMAN

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Protein
Beta-1,4-galactosyltransferase 4
Gene
B4GALT4, UNQ552/PRO1109
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.1 Publication
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Manganese By similarity.

Kineticsi

  1. KM=238 µM for GlcNAc-B-S-pNP1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961Manganese By similarity
Binding sitei224 – 2241UDP-alpha-D-galactose By similarity
Binding sitei256 – 2561UDP-alpha-D-galactose By similarity
Metal bindingi289 – 2891Manganese; via tele nitrogen By similarity
Binding sitei301 – 3011N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. N-acetyllactosamine synthase activity Source: UniProtKB-EC
  2. galactosyltransferase activity Source: ProtInc
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. cellular protein metabolic process Source: Reactome
  3. glycosaminoglycan metabolic process Source: Reactome
  4. keratan sulfate biosynthetic process Source: Reactome
  5. keratan sulfate metabolic process Source: Reactome
  6. membrane lipid metabolic process Source: ProtInc
  7. post-translational protein modification Source: Reactome
  8. protein N-linked glycosylation via asparagine Source: Reactome
  9. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04504-MONOMER.
ReactomeiREACT_121120. Keratan sulfate biosynthesis.
REACT_25085. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4GALT4
ORF Names:UNQ552/PRO1109
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:927. B4GALT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 3826Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini39 – 344306Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25226.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi77 ↔ 118 By similarity
Disulfide bondi189 ↔ 208 By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi335 – 3351N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO60513.
PaxDbiO60513.
PRIDEiO60513.

PTM databases

PhosphoSiteiO60513.

Expressioni

Tissue specificityi

High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Gene expression databases

ArrayExpressiO60513.
BgeeiO60513.
CleanExiHS_B4GALT4.
GenevestigatoriO60513.

Organism-specific databases

HPAiHPA046819.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000352144.

Structurei

3D structure databases

ProteinModelPortaliO60513.
SMRiO60513. Positions 77-339.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1335UDP-alpha-D-galactose binding By similarity
Regioni168 – 1703UDP-alpha-D-galactose binding By similarity
Regioni195 – 1962UDP-alpha-D-galactose binding By similarity
Regioni258 – 2614N-acetyl-D-glucosamine binding By similarity
Regioni289 – 2913UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO60513.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG7060R0.
PhylomeDBiO60513.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O60513-1 [UniParc]FASTAAdd to Basket

« Hide

MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN    50
FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE 100
VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR 150
QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE 200
NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS 250
NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE 300
RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA 344
Length:344
Mass (Da):40,041
Last modified:August 1, 1998 - v1
Checksum:i6B7742676FE8A58B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti116 – 1161Q → E.3 Publications
Corresponds to variant rs3764779 [ dbSNP | Ensembl ].
VAR_022697

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821L → R in AAG50147. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF038662 mRNA. Translation: AAC39735.1.
AF022367 mRNA. Translation: AAC72493.1.
AB024436 mRNA. Translation: BAA75821.1.
AF020920 mRNA. Translation: AAG50147.1.
AY359008 mRNA. Translation: AAQ89367.1.
CR749555 mRNA. Translation: CAH18352.1.
BC004523 mRNA. Translation: AAH04523.1.
BC062618 mRNA. Translation: AAH62618.1.
CCDSiCCDS2986.1.
RefSeqiNP_003769.1. NM_003778.3.
NP_997708.1. NM_212543.1.
XP_005247912.1. XM_005247855.1.
XP_006713861.1. XM_006713798.1.
XP_006713862.1. XM_006713799.1.
XP_006713863.1. XM_006713800.1.
XP_006713864.1. XM_006713801.1.
UniGeneiHs.13225.

Genome annotation databases

EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
ENST00000393765; ENSP00000377360; ENSG00000121578.
ENST00000483209; ENSP00000420161; ENSG00000121578.
GeneIDi8702.
KEGGihsa:8702.
UCSCiuc003ece.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 4

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF038662 mRNA. Translation: AAC39735.1 .
AF022367 mRNA. Translation: AAC72493.1 .
AB024436 mRNA. Translation: BAA75821.1 .
AF020920 mRNA. Translation: AAG50147.1 .
AY359008 mRNA. Translation: AAQ89367.1 .
CR749555 mRNA. Translation: CAH18352.1 .
BC004523 mRNA. Translation: AAH04523.1 .
BC062618 mRNA. Translation: AAH62618.1 .
CCDSi CCDS2986.1.
RefSeqi NP_003769.1. NM_003778.3.
NP_997708.1. NM_212543.1.
XP_005247912.1. XM_005247855.1.
XP_006713861.1. XM_006713798.1.
XP_006713862.1. XM_006713799.1.
XP_006713863.1. XM_006713800.1.
XP_006713864.1. XM_006713801.1.
UniGenei Hs.13225.

3D structure databases

ProteinModelPortali O60513.
SMRi O60513. Positions 77-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000352144.

Chemistry

DrugBanki DB00141. N-Acetyl-D-glucosamine.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei O60513.

Proteomic databases

MaxQBi O60513.
PaxDbi O60513.
PRIDEi O60513.

Protocols and materials databases

DNASUi 8702.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000359213 ; ENSP00000352144 ; ENSG00000121578 .
ENST00000393765 ; ENSP00000377360 ; ENSG00000121578 .
ENST00000483209 ; ENSP00000420161 ; ENSG00000121578 .
GeneIDi 8702.
KEGGi hsa:8702.
UCSCi uc003ece.1. human.

Organism-specific databases

CTDi 8702.
GeneCardsi GC03M118930.
HGNCi HGNC:927. B4GALT4.
HPAi HPA046819.
MIMi 604015. gene.
neXtProti NX_O60513.
PharmGKBi PA25226.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG327897.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi O60513.
KOi K07969.
OMAi HTRDKGN.
OrthoDBi EOG7060R0.
PhylomeDBi O60513.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:HS04504-MONOMER.
Reactomei REACT_121120. Keratan sulfate biosynthesis.
REACT_25085. N-Glycan antennae elongation.

Miscellaneous databases

GeneWikii B4GALT4.
GenomeRNAii 8702.
NextBioi 32625.
PROi O60513.
SOURCEi Search...

Gene expression databases

ArrayExpressi O60513.
Bgeei O60513.
CleanExi HS_B4GALT4.
Genevestigatori O60513.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis."
    Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H.
    J. Biol. Chem. 273:29331-29340(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
  3. "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
    Guo S., Sato T., Shirane K., Furukawa K.
    Glycobiology 11:813-820(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family."
    Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S.
    Sci. China, Ser. C, Life Sci. 42:337-345(1999)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
    Tissue: Fetal kidney.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
    Tissue: Colon and Muscle.
  8. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
    Amado M., Almeida R., Schwientek T., Clausen H.
    Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiB4GT4_HUMAN
AccessioniPrimary (citable) accession number: O60513
Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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