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Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.1 Publication
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=238 µM for GlcNAc-B-S-pNP1 Publication

    Pathway:iprotein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi196 – 1961ManganeseBy similarity
    Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
    Binding sitei256 – 2561UDP-alpha-D-galactoseBy similarity
    Metal bindingi289 – 2891Manganese; via tele nitrogenBy similarity
    Binding sitei301 – 3011N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    • galactosyltransferase activity Source: ProtInc
    • metal ion binding Source: UniProtKB-KW
    • N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS04504-MONOMER.
    BRENDAi2.4.1.275. 2681.
    ReactomeiREACT_121120. Keratan sulfate biosynthesis.
    REACT_25085. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 4
    Short name:
    Beta4Gal-T4
    Short name:
    b4Gal-T4
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    Including the following 2 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4GALT4
    ORF Names:UNQ552/PRO1109
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:927. B4GALT4.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei13 – 3826Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST
    Topological domaini39 – 344306LumenalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • Golgi cisterna membrane Source: UniProtKB-SubCell
    • Golgi membrane Source: Reactome
    • integral component of membrane Source: ProtInc
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25226.

    Chemistry

    DrugBankiDB00141. N-Acetyl-D-glucosamine.

    Polymorphism and mutation databases

    BioMutaiB4GALT4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Beta-1,4-galactosyltransferase 4PRO_0000080542Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi77 ↔ 118By similarity
    Disulfide bondi189 ↔ 208By similarity
    Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO60513.
    PaxDbiO60513.
    PRIDEiO60513.

    PTM databases

    PhosphoSiteiO60513.

    Expressioni

    Tissue specificityi

    High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

    Gene expression databases

    BgeeiO60513.
    CleanExiHS_B4GALT4.
    ExpressionAtlasiO60513. baseline and differential.
    GenevisibleiO60513. HS.

    Organism-specific databases

    HPAiHPA046819.

    Interactioni

    Protein-protein interaction databases

    BioGridi114245. 7 interactions.
    STRINGi9606.ENSP00000352144.

    Structurei

    3D structure databases

    ProteinModelPortaliO60513.
    SMRiO60513. Positions 77-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1335UDP-alpha-D-galactose bindingBy similarity
    Regioni168 – 1703UDP-alpha-D-galactose bindingBy similarity
    Regioni195 – 1962UDP-alpha-D-galactose bindingBy similarity
    Regioni258 – 2614N-acetyl-D-glucosamine bindingBy similarity
    Regioni289 – 2913UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00760000119140.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO60513.
    KOiK07969.
    OMAiHTRDKGN.
    OrthoDBiEOG7060R0.
    PhylomeDBiO60513.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O60513-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN
    60 70 80 90 100
    FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE
    110 120 130 140 150
    VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR
    160 170 180 190 200
    QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE
    210 220 230 240 250
    NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS
    260 270 280 290 300
    NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE
    310 320 330 340
    RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA
    Length:344
    Mass (Da):40,041
    Last modified:August 1, 1998 - v1
    Checksum:i6B7742676FE8A58B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1821L → R in AAG50147 (Ref. 4) Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti116 – 1161Q → E.3 Publications
    Corresponds to variant rs3764779 [ dbSNP | Ensembl ].
    VAR_022697

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF038662 mRNA. Translation: AAC39735.1.
    AF022367 mRNA. Translation: AAC72493.1.
    AB024436 mRNA. Translation: BAA75821.1.
    AF020920 mRNA. Translation: AAG50147.1.
    AY359008 mRNA. Translation: AAQ89367.1.
    CR749555 mRNA. Translation: CAH18352.1.
    BC004523 mRNA. Translation: AAH04523.1.
    BC062618 mRNA. Translation: AAH62618.1.
    CCDSiCCDS2986.1.
    RefSeqiNP_003769.1. NM_003778.3.
    NP_997708.1. NM_212543.1.
    XP_005247912.1. XM_005247855.1.
    XP_006713861.1. XM_006713798.2.
    XP_006713862.1. XM_006713799.2.
    XP_006713863.1. XM_006713800.1.
    XP_006713864.1. XM_006713801.2.
    XP_011511562.1. XM_011513260.1.
    UniGeneiHs.13225.

    Genome annotation databases

    EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
    ENST00000393765; ENSP00000377360; ENSG00000121578.
    ENST00000483209; ENSP00000420161; ENSG00000121578.
    GeneIDi8702.
    KEGGihsa:8702.
    UCSCiuc003ece.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Beta-1,4-galactosyltransferase 4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF038662 mRNA. Translation: AAC39735.1.
    AF022367 mRNA. Translation: AAC72493.1.
    AB024436 mRNA. Translation: BAA75821.1.
    AF020920 mRNA. Translation: AAG50147.1.
    AY359008 mRNA. Translation: AAQ89367.1.
    CR749555 mRNA. Translation: CAH18352.1.
    BC004523 mRNA. Translation: AAH04523.1.
    BC062618 mRNA. Translation: AAH62618.1.
    CCDSiCCDS2986.1.
    RefSeqiNP_003769.1. NM_003778.3.
    NP_997708.1. NM_212543.1.
    XP_005247912.1. XM_005247855.1.
    XP_006713861.1. XM_006713798.2.
    XP_006713862.1. XM_006713799.2.
    XP_006713863.1. XM_006713800.1.
    XP_006713864.1. XM_006713801.2.
    XP_011511562.1. XM_011513260.1.
    UniGeneiHs.13225.

    3D structure databases

    ProteinModelPortaliO60513.
    SMRiO60513. Positions 77-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114245. 7 interactions.
    STRINGi9606.ENSP00000352144.

    Chemistry

    DrugBankiDB00141. N-Acetyl-D-glucosamine.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSiteiO60513.

    Polymorphism and mutation databases

    BioMutaiB4GALT4.

    Proteomic databases

    MaxQBiO60513.
    PaxDbiO60513.
    PRIDEiO60513.

    Protocols and materials databases

    DNASUi8702.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000359213; ENSP00000352144; ENSG00000121578.
    ENST00000393765; ENSP00000377360; ENSG00000121578.
    ENST00000483209; ENSP00000420161; ENSG00000121578.
    GeneIDi8702.
    KEGGihsa:8702.
    UCSCiuc003ece.1. human.

    Organism-specific databases

    CTDi8702.
    GeneCardsiGC03M118930.
    HGNCiHGNC:927. B4GALT4.
    HPAiHPA046819.
    MIMi604015. gene.
    neXtProtiNX_O60513.
    PharmGKBiPA25226.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00760000119140.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO60513.
    KOiK07969.
    OMAiHTRDKGN.
    OrthoDBiEOG7060R0.
    PhylomeDBiO60513.
    TreeFamiTF312834.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BioCyciMetaCyc:HS04504-MONOMER.
    BRENDAi2.4.1.275. 2681.
    ReactomeiREACT_121120. Keratan sulfate biosynthesis.
    REACT_25085. N-Glycan antennae elongation.

    Miscellaneous databases

    GeneWikiiB4GALT4.
    GenomeRNAii8702.
    NextBioi32625.
    PROiO60513.
    SOURCEiSearch...

    Gene expression databases

    BgeeiO60513.
    CleanExiHS_B4GALT4.
    ExpressionAtlasiO60513. baseline and differential.
    GenevisibleiO60513. HS.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
      Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
      Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis."
      Schwientek T., Almeida R., Levery S.B., Holmes E.H., Bennett E., Clausen H.
      J. Biol. Chem. 273:29331-29340(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    3. "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
      Guo S., Sato T., Shirane K., Furukawa K.
      Glycobiology 11:813-820(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Cloning and characterization of a novel member of the human beta-1,4-galactosyltransferase gene family."
      Fan Y., Yu L., Zhang Q., Jiang Y., Dai F., Chen C., Tu Q., Bi A., Xu Y., Zhao S.
      Sci. China, Ser. C, Life Sci. 42:337-345(1999)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLU-116.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
      Tissue: Fetal kidney.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-116.
      Tissue: Colon and Muscle.
    8. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
      Amado M., Almeida R., Schwientek T., Clausen H.
      Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiB4GT4_HUMAN
    AccessioniPrimary (citable) accession number: O60513
    Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: August 1, 1998
    Last modified: July 22, 2015
    This is version 149 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.