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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4GALT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=63 µM for GlcNAc-B-S-pNP1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi197 – 1971ManganeseBy similarity
    Binding sitei226 – 2261UDP-alpha-D-galactoseBy similarity
    Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
    Metal bindingi291 – 2911Manganese; via tele nitrogenBy similarity
    Binding sitei303 – 3031N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
    R-HSA-975577. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Chemistry

    SwissLipidsiSLP:000000790.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 3
    Short name:
    Beta4Gal-T3
    Short name:
    b4Gal-T3
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    Including the following 3 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4GALT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:926. B4GALT3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010CytoplasmicSequence analysis
    Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
    BLAST
    Topological domaini32 – 393362LumenalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25225.

    Chemistry

    DrugBankiDB00141. N-Acetyl-D-glucosamine.

    Polymorphism and mutation databases

    BioMutaiB4GALT3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Beta-1,4-galactosyltransferase 3PRO_0000080537Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
    Disulfide bondi77 ↔ 119By similarity
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
    Disulfide bondi190 ↔ 209By similarity
    Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence analysis
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiO60512.
    MaxQBiO60512.
    PaxDbiO60512.
    PeptideAtlasiO60512.
    PRIDEiO60512.

    PTM databases

    iPTMnetiO60512.
    PhosphoSiteiO60512.

    Expressioni

    Tissue specificityi

    Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.

    Gene expression databases

    BgeeiENSG00000158850.
    CleanExiHS_B4GALT3.
    GenevisibleiO60512. HS.

    Organism-specific databases

    HPAiHPA010793.

    Interactioni

    Protein-protein interaction databases

    BioGridi114246. 31 interactions.
    IntActiO60512. 5 interactions.
    MINTiMINT-1371449.
    STRINGi9606.ENSP00000320965.

    Structurei

    3D structure databases

    ProteinModelPortaliO60512.
    SMRiO60512. Positions 74-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1345UDP-alpha-D-galactose bindingBy similarity
    Regioni169 – 1713UDP-alpha-D-galactose bindingBy similarity
    Regioni196 – 1972UDP-alpha-D-galactose bindingBy similarity
    Regioni260 – 2634N-acetyl-D-glucosamine bindingBy similarity
    Regioni291 – 2933UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3916. Eukaryota.
    ENOG410ZYYA. LUCA.
    GeneTreeiENSGT00760000119140.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO60512.
    KOiK07968.
    OMAiIGFQFAF.
    OrthoDBiEOG091G0P66.
    PhylomeDBiO60512.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: O60512-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH
    60 70 80 90 100
    PRDVYSNLSH LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE
    110 120 130 140 150
    IVERNPRVEP GGRYRPAGCE PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ
    160 170 180 190 200
    RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR EALRDEEWDC LFLHDVDLLP
    210 220 230 240 250
    ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL TPDQYLKMNG
    260 270 280 290 300
    FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN
    310 320 330 340 350
    PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR
    360 370 380 390
    APSGPRYPPG SSQAFRQEML QRRPPARPGP LSTANHTALR GSH
    Length:393
    Mass (Da):43,928
    Last modified:June 21, 2005 - v2
    Checksum:iCA0BF955F95ED1F4
    GO
    Isoform 2 (identifier: O60512-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         116-145: PAGCEPRSRTAIIVPHRAREHHLRLLLYHL → PAALPPAPLLAAPAACLWHLCHPPGWKWNI
         146-393: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:145
    Mass (Da):15,654
    Checksum:i4231F9B50756A99C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641A → R in AAC39734 (PubMed:9597550).Curated
    Sequence conflicti112 – 1121G → A in AAC39734 (PubMed:9597550).Curated
    Sequence conflicti223 – 2231K → S in AAC39734 (PubMed:9597550).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei116 – 14530PAGCE…LLYHL → PAALPPAPLLAAPAACLWHL CHPPGWKWNI in isoform 2. 1 PublicationVSP_014106Add
    BLAST
    Alternative sequencei146 – 393248Missing in isoform 2. 1 PublicationVSP_014107Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y12509 mRNA. Translation: CAA73111.1.
    AF038661 mRNA. Translation: AAC39734.1.
    AB024435 mRNA. Translation: BAA75820.1.
    AL590714 Genomic DNA. Translation: CAH72145.1.
    AK023311 mRNA. Translation: BAB14520.1.
    CH471121 Genomic DNA. Translation: EAW52628.1.
    CH471121 Genomic DNA. Translation: EAW52629.1.
    CH471121 Genomic DNA. Translation: EAW52630.1.
    CH471121 Genomic DNA. Translation: EAW52631.1.
    CH471121 Genomic DNA. Translation: EAW52632.1.
    BC000276 mRNA. Translation: AAH00276.1.
    BC006099 mRNA. Translation: AAH06099.1.
    BC009985 mRNA. Translation: AAH09985.1.
    CCDSiCCDS1222.1. [O60512-1]
    RefSeqiNP_001186802.1. NM_001199873.1. [O60512-1]
    NP_001186803.1. NM_001199874.1. [O60512-1]
    NP_003770.1. NM_003779.3. [O60512-1]
    XP_005245623.1. XM_005245566.1. [O60512-1]
    UniGeneiHs.321231.
    Hs.741821.

    Genome annotation databases

    EnsembliENST00000319769; ENSP00000320965; ENSG00000158850. [O60512-1]
    ENST00000367998; ENSP00000356977; ENSG00000158850. [O60512-1]
    ENST00000622395; ENSP00000480428; ENSG00000158850. [O60512-1]
    GeneIDi8703.
    KEGGihsa:8703.
    UCSCiuc001fyq.3. human. [O60512-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Beta-1,4-galactosyltransferase 3

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y12509 mRNA. Translation: CAA73111.1.
    AF038661 mRNA. Translation: AAC39734.1.
    AB024435 mRNA. Translation: BAA75820.1.
    AL590714 Genomic DNA. Translation: CAH72145.1.
    AK023311 mRNA. Translation: BAB14520.1.
    CH471121 Genomic DNA. Translation: EAW52628.1.
    CH471121 Genomic DNA. Translation: EAW52629.1.
    CH471121 Genomic DNA. Translation: EAW52630.1.
    CH471121 Genomic DNA. Translation: EAW52631.1.
    CH471121 Genomic DNA. Translation: EAW52632.1.
    BC000276 mRNA. Translation: AAH00276.1.
    BC006099 mRNA. Translation: AAH06099.1.
    BC009985 mRNA. Translation: AAH09985.1.
    CCDSiCCDS1222.1. [O60512-1]
    RefSeqiNP_001186802.1. NM_001199873.1. [O60512-1]
    NP_001186803.1. NM_001199874.1. [O60512-1]
    NP_003770.1. NM_003779.3. [O60512-1]
    XP_005245623.1. XM_005245566.1. [O60512-1]
    UniGeneiHs.321231.
    Hs.741821.

    3D structure databases

    ProteinModelPortaliO60512.
    SMRiO60512. Positions 74-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114246. 31 interactions.
    IntActiO60512. 5 interactions.
    MINTiMINT-1371449.
    STRINGi9606.ENSP00000320965.

    Chemistry

    DrugBankiDB00141. N-Acetyl-D-glucosamine.
    SwissLipidsiSLP:000000790.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    PTM databases

    iPTMnetiO60512.
    PhosphoSiteiO60512.

    Polymorphism and mutation databases

    BioMutaiB4GALT3.

    Proteomic databases

    EPDiO60512.
    MaxQBiO60512.
    PaxDbiO60512.
    PeptideAtlasiO60512.
    PRIDEiO60512.

    Protocols and materials databases

    DNASUi8703.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000319769; ENSP00000320965; ENSG00000158850. [O60512-1]
    ENST00000367998; ENSP00000356977; ENSG00000158850. [O60512-1]
    ENST00000622395; ENSP00000480428; ENSG00000158850. [O60512-1]
    GeneIDi8703.
    KEGGihsa:8703.
    UCSCiuc001fyq.3. human. [O60512-1]

    Organism-specific databases

    CTDi8703.
    GeneCardsiB4GALT3.
    H-InvDBHIX0159958.
    HGNCiHGNC:926. B4GALT3.
    HPAiHPA010793.
    MIMi604014. gene.
    neXtProtiNX_O60512.
    PharmGKBiPA25225.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3916. Eukaryota.
    ENOG410ZYYA. LUCA.
    GeneTreeiENSGT00760000119140.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiO60512.
    KOiK07968.
    OMAiIGFQFAF.
    OrthoDBiEOG091G0P66.
    PhylomeDBiO60512.
    TreeFamiTF312834.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
    R-HSA-975577. N-Glycan antennae elongation.

    Miscellaneous databases

    GeneWikiiB4GALT3.
    GenomeRNAii8703.
    PROiO60512.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000158850.
    CleanExiHS_B4GALT3.
    GenevisibleiO60512. HS.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiB4GT3_HUMAN
    AccessioniPrimary (citable) accession number: O60512
    Secondary accession number(s): D3DVG3
    , O60910, Q9BPZ4, Q9H8T2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: June 21, 2005
    Last modified: September 7, 2016
    This is version 151 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.