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O60508

- PRP17_HUMAN

UniProt

O60508 - PRP17_HUMAN

Protein

Pre-mRNA-processing factor 17

Gene

CDC40

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.1 Publication

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. gene expression Source: Reactome
    2. mRNA 3'-end processing Source: Reactome
    3. mRNA export from nucleus Source: Reactome
    4. mRNA splicing, via spliceosome Source: UniProtKB
    5. RNA splicing Source: Reactome
    6. termination of RNA polymerase II transcription Source: Reactome
    7. transcription from RNA polymerase II promoter Source: Reactome

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    ReactomeiREACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1849. mRNA 3'-end processing.
    REACT_467. mRNA Splicing - Major Pathway.
    SignaLinkiO60508.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-processing factor 17
    Alternative name(s):
    Cell division cycle 40 homolog
    EH-binding protein 3
    Short name:
    Ehb3
    PRP17 homolog
    Short name:
    hPRP17
    Gene namesi
    Name:CDC40
    Synonyms:EHB3, PRP17, PRPF17
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:17350. CDC40.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. spliceosomal complex Source: ProtInc

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134891332.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 579579Pre-mRNA-processing factor 17PRO_0000051142Add
    BLAST

    Proteomic databases

    MaxQBiO60508.
    PaxDbiO60508.
    PRIDEiO60508.

    PTM databases

    PhosphoSiteiO60508.

    Miscellaneous databases

    PMAP-CutDBO60508.

    Expressioni

    Gene expression databases

    ArrayExpressiO60508.
    BgeeiO60508.
    CleanExiHS_CDC40.
    GenevestigatoriO60508.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRPF19Q9UMS42EBI-2557812,EBI-395746

    Protein-protein interaction databases

    BioGridi119497. 21 interactions.
    IntActiO60508. 4 interactions.
    MINTiMINT-3000088.
    STRINGi9606.ENSP00000304370.

    Structurei

    3D structure databases

    ProteinModelPortaliO60508.
    SMRiO60508. Positions 280-578.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati286 – 32641WD 1Add
    BLAST
    Repeati330 – 36940WD 2Add
    BLAST
    Repeati371 – 41343WD 3Add
    BLAST
    Repeati416 – 45540WD 4Add
    BLAST
    Repeati459 – 49840WD 5Add
    BLAST
    Repeati504 – 54542WD 6Add
    BLAST
    Repeati548 – 57831WD 7Add
    BLAST

    Sequence similaritiesi

    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000214672.
    HOVERGENiHBG053695.
    InParanoidiO60508.
    KOiK12816.
    OMAiGPENPFG.
    OrthoDBiEOG7KM5S8.
    PhylomeDBiO60508.
    TreeFamiTF101064.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 6 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60508-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL    50
    AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMFA PEFGPENPFR 100
    TQQMAAPRNM LSGYAEPAHI NDFMFEQQRR TFATYGYALD PSLDNHQVSA 150
    KYIGSVEEAE KNQGLTVFET GQKKTEKRKK FKENDASNID GFLGPWAKYV 200
    DEKDVAKPSE EEQKELDEIT AKRQKKGKQE EEKPGEEKTI LHVKEMYDYQ 250
    GRSYLHIPQD VGVNLRSTMP PEKCYLPKKQ IHVWSGHTKG VSAVRLFPLS 300
    GHLLLSCSMD CKIKLWEVYG ERRCLRTFIG HSKAVRDICF NTAGTQFLSA 350
    AYDRYLKLWD TETGQCISRF TNRKVPYCVK FNPDEDKQNL FVAGMSDKKI 400
    VQWDIRSGEI VQEYDRHLGA VNTIVFVDEN RRFVSTSDDK SLRVWEWDIP 450
    VDFKYIAEPS MHSMPAVTLS PNGKWLACQS MDNQILIFGA QNRFRLNKKK 500
    IFKGHMVAGY ACQVDFSPDM SYVISGDGNG KLNIWDWKTT KLYSRFKAHD 550
    KVCIGAVWHP HETSKVITCG WDGLIKLWD 579
    Length:579
    Mass (Da):65,521
    Last modified:August 1, 1998 - v1
    Checksum:i1DE2BAA6F03FF16D
    GO

    Sequence cautioni

    The sequence AAC25166.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71A → V in AAD01552. (PubMed:9303539)Curated
    Sequence conflicti31 – 311L → P in AAC25166. (PubMed:9769104)Curated
    Sequence conflicti210 – 2101E → R in AAC25166. (PubMed:9769104)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038392 mRNA. Translation: AAC39730.1.
    AL671518 Genomic DNA. Translation: CAI13322.1.
    AK314601 mRNA. Translation: BAG37172.1.
    BC117145 mRNA. Translation: AAI17146.1.
    BC126114 mRNA. Translation: AAI26115.1.
    AF061241 mRNA. Translation: AAC25166.1. Different initiation.
    AF015044 mRNA. Translation: AAD01552.1.
    CCDSiCCDS5081.1.
    RefSeqiNP_056975.1. NM_015891.2.
    UniGeneiHs.428147.

    Genome annotation databases

    EnsembliENST00000307731; ENSP00000304370; ENSG00000168438.
    ENST00000368932; ENSP00000357928; ENSG00000168438.
    GeneIDi51362.
    KEGGihsa:51362.
    UCSCiuc003pua.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF038392 mRNA. Translation: AAC39730.1 .
    AL671518 Genomic DNA. Translation: CAI13322.1 .
    AK314601 mRNA. Translation: BAG37172.1 .
    BC117145 mRNA. Translation: AAI17146.1 .
    BC126114 mRNA. Translation: AAI26115.1 .
    AF061241 mRNA. Translation: AAC25166.1 . Different initiation.
    AF015044 mRNA. Translation: AAD01552.1 .
    CCDSi CCDS5081.1.
    RefSeqi NP_056975.1. NM_015891.2.
    UniGenei Hs.428147.

    3D structure databases

    ProteinModelPortali O60508.
    SMRi O60508. Positions 280-578.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119497. 21 interactions.
    IntActi O60508. 4 interactions.
    MINTi MINT-3000088.
    STRINGi 9606.ENSP00000304370.

    PTM databases

    PhosphoSitei O60508.

    Proteomic databases

    MaxQBi O60508.
    PaxDbi O60508.
    PRIDEi O60508.

    Protocols and materials databases

    DNASUi 51362.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307731 ; ENSP00000304370 ; ENSG00000168438 .
    ENST00000368932 ; ENSP00000357928 ; ENSG00000168438 .
    GeneIDi 51362.
    KEGGi hsa:51362.
    UCSCi uc003pua.3. human.

    Organism-specific databases

    CTDi 51362.
    GeneCardsi GC06P110547.
    HGNCi HGNC:17350. CDC40.
    MIMi 605585. gene.
    neXtProti NX_O60508.
    PharmGKBi PA134891332.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000214672.
    HOVERGENi HBG053695.
    InParanoidi O60508.
    KOi K12816.
    OMAi GPENPFG.
    OrthoDBi EOG7KM5S8.
    PhylomeDBi O60508.
    TreeFami TF101064.

    Enzyme and pathway databases

    Reactomei REACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1849. mRNA 3'-end processing.
    REACT_467. mRNA Splicing - Major Pathway.
    SignaLinki O60508.

    Miscellaneous databases

    GeneWikii CDC40.
    GenomeRNAii 51362.
    NextBioi 54822.
    PMAP-CutDB O60508.
    PROi O60508.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60508.
    Bgeei O60508.
    CleanExi HS_CDC40.
    Genevestigatori O60508.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 6 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing."
      Zhou Z., Reed R.
      EMBO J. 17:2095-2106(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Colon epithelium.
    2. "Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p."
      Lindsey L.A., Garcia-Blanco M.A.
      J. Biol. Chem. 273:32771-32775(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Cervix carcinoma.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    4. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Identification and functional analysis of hPRP17, the human homologue of the PRP17/CDC40 yeast gene involved in splicing and cell cycle control."
      Ben-Yehuda S., Dix I., Russell C.S., Levy S., Beggs J.D., Kupiec M.
      RNA 4:1304-1312(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-579.
    7. "Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module."
      Salcini A.E., Confalonieri S., Doria M., Santolini E., Tassi E., Minenkova O., Cesareni G., Pelicci P.G., Di Fiore P.P.
      Genes Dev. 11:2239-2249(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-173.
    8. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRP17_HUMAN
    AccessioniPrimary (citable) accession number: O60508
    Secondary accession number(s): B2RBC5
    , O75471, Q5SRN0, Q9UPG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3