Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-tyrosine sulfotransferase 1

Gene

TPST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.3 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Proton donor/acceptorBy similarity1
Sitei159Transition state stabilizerBy similarity1
Binding sitei184PAPSCombined sources1 Publication1
Binding sitei192PAPSCombined sources1 Publication1
Binding sitei196PAPSCombined sources1 Publication1
Binding sitei239PAPSCombined sources1 Publication1
Sitei286Transition state stabilizerBy similarity1
Binding sitei301PAPSCombined sources1 Publication1

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein-tyrosine sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
  • inflammatory response Source: ProtInc
  • peptidyl-tyrosine sulfation Source: UniProtKB

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:HS10030-MONOMER.
BRENDAi2.8.2.20. 2681.
ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 1 (EC:2.8.2.203 Publications)
Alternative name(s):
Tyrosylprotein sulfotransferase 1
Short name:
TPST-1
Gene namesi
Name:TPST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000169902.13.
HGNCiHGNC:12020. TPST1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini26 – 370LumenalSequence analysisAdd BLAST345

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60N → A: Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-262. 1 Publication1
Mutagenesisi262N → A: Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-60. 1 Publication1

Organism-specific databases

DisGeNETi8460.
OpenTargetsiENSG00000169902.
PharmGKBiPA36699.

Polymorphism and mutation databases

BioMutaiTPST1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001898261 – 370Protein-tyrosine sulfotransferase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi97 ↔ 1571 Publication
Disulfide bondi226 ↔ 2341 Publication
Glycosylationi262N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO60507.
MaxQBiO60507.
PaxDbiO60507.
PeptideAtlasiO60507.
PRIDEiO60507.

PTM databases

iPTMnetiO60507.
PhosphoSitePlusiO60507.

Expressioni

Tissue specificityi

Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000169902.
CleanExiHS_TPST1.
ExpressionAtlasiO60507. baseline and differential.
GenevisibleiO60507. HS.

Organism-specific databases

HPAiHPA047415.

Interactioni

Subunit structurei

Homodimer (PubMed:16859706, PubMed:28821720). Can also form heterodimers with TPST2 (PubMed:25660941).3 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi114038. 6 interactors.
STRINGi9606.ENSP00000302413.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi71 – 75Combined sources5
Beta strandi77 – 81Combined sources5
Helixi82 – 90Combined sources9
Helixi103 – 115Combined sources13
Helixi118 – 126Combined sources9
Helixi131 – 148Combined sources18
Beta strandi154 – 159Combined sources6
Helixi161 – 166Combined sources6
Helixi167 – 173Combined sources7
Beta strandi177 – 183Combined sources7
Helixi186 – 196Combined sources11
Helixi208 – 229Combined sources22
Turni231 – 233Combined sources3
Beta strandi234 – 238Combined sources5
Helixi239 – 244Combined sources6
Helixi246 – 257Combined sources12
Helixi263 – 271Combined sources9
Helixi287 – 290Combined sources4
Turni301 – 305Combined sources5
Helixi308 – 312Combined sources5
Helixi314 – 317Combined sources4
Helixi320 – 323Combined sources4
Beta strandi330 – 332Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WRIX-ray1.60A/B43-341[»]
5WRJX-ray2.31A/B/C/D43-341[»]
ProteinModelPortaliO60507.
SMRiO60507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 83PAPS bindingCombined sources1 Publication5
Regioni102 – 106Interaction with peptide substrate1 Publication5
Regioni286 – 295PAPS bindingCombined sources1 Publication10

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988. Eukaryota.
ENOG410XQ2X. LUCA.
GeneTreeiENSGT00390000006030.
HOGENOMiHOG000253020.
HOVERGENiHBG001270.
InParanoidiO60507.
KOiK01021.
OMAiHIPWNHS.
OrthoDBiEOG091G09YJ.
PhylomeDBiO60507.
TreeFamiTF312910.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR026634. TPST.
PANTHERiPTHR12788. PTHR12788. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

O60507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PVKLESTRTT
60 70 80 90 100
VRTGLDLKAN KTFAYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE
110 120 130 140 150
TRVIPRILAL KQMWSRSSKE KIRLDEAGVT DEVLDSAMQA FLLEIIVKHG
160 170 180 190 200
EPAPYLCNKD PFALKSLTYL SRLFPNAKFL LMVRDGRASV HSMISRKVTI
210 220 230 240 250
AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE QLVLHPERWM
260 270 280 290 300
RTLLKFLQIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
310 320 330 340 350
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KIIENTRRVY
360 370
KGEFQLPDFL KEKPQTEQVE
Length:370
Mass (Da):42,188
Last modified:August 1, 1998 - v1
Checksum:iFB9EB341AFE36407
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038009 mRNA. Translation: AAC13552.1.
AK313098 mRNA. Translation: BAG35922.1.
CR542060 mRNA. Translation: CAG46857.1.
CR542080 mRNA. Translation: CAG46877.1.
CH236961 Genomic DNA. Translation: EAL23739.1.
CH471140 Genomic DNA. Translation: EAX07937.1.
BC013188 mRNA. Translation: AAH13188.1.
CCDSiCCDS5533.1.
RefSeqiNP_003587.1. NM_003596.3.
XP_016868214.1. XM_017012725.1.
XP_016868215.1. XM_017012726.1.
XP_016868216.1. XM_017012727.1.
UniGeneiHs.421194.

Genome annotation databases

EnsembliENST00000304842; ENSP00000302413; ENSG00000169902.
GeneIDi8460.
KEGGihsa:8460.
UCSCiuc003tuw.4. human.

Similar proteinsi

Entry informationi

Entry nameiTPST1_HUMAN
AccessioniPrimary (citable) accession number: O60507
Secondary accession number(s): A4D2M0, Q6FGM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 22, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families