Reviewed, UniProtKB/Swiss-Prot O60507 (TPST1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein-tyrosine sulfotransferase 1
    EC=2.8.2.20
Alternative name(s):
    Tyrosylprotein sulfotransferase-1
      Short name=TPST-1

Gene names

Name:

TPST1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Catalytic activity	3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity.
Sequence similarities	Belongs to the protein sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	inflammatory response Ref.1 Traceable author statement. Source: ProtInc peptidyl-tyrosine sulfation Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein-tyrosine sulfotransferase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Protein-tyrosine sulfotransferase 1		PRO_0000189826
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 25	17	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 370	345	Lumenal Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O60507-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FB9EB341AFE36407	FASTA	370	42,188
	10 20 30 40 50 60 MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PVKLESTRTT VRTGLDLKAN 70 80 90 100 110 120 KTFAYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE TRVIPRILAL KQMWSRSSKE 130 140 150 160 170 180 KIRLDEAGVT DEVLDSAMQA FLLEIIVKHG EPAPYLCNKD PFALKSLTYL SRLFPNAKFL 190 200 210 220 230 240 LMVRDGRASV HSMISRKVTI AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE 250 260 270 280 290 300 QLVLHPERWM RTLLKFLQIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS 310 320 330 340 350 360 KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KIIENTRRVY KGEFQLPDFL 370 KEKPQTEQVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins." Ouyang Y.-B., Lane W.S., Moore K.L. Proc. Natl. Acad. Sci. U.S.A. 95:2896-2901(1998) [PubMed: 9501187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038009 mRNA. Translation: AAC13552.1. CR542060 mRNA. Translation: CAG46857.1. CR542080 mRNA. Translation: CAG46877.1. BC013188 mRNA. Translation: AAH13188.1.
RefSeq	NP_003587.1.
UniGene	Hs.421194
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000169902. Homo sapiens. [Contig view]
GeneID	8460.
KEGG	hsa:8460.
Organism-specific databases
H-InvDB	HIX0006719.
HGNC	HGNC:12020. TPST1.
MIM	603125. gene.
PharmGKB	PA36699.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	O60507.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12487.
Gene expression databases
ArrayExpress	O60507.
CleanEx	HS_TPST1.
GermOnline	ENSG00000169902. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	31664.
SOURCE	Search...

Entry information

Entry name

TPST1_HUMAN

Accession

Primary (citable) accession number: O60507
Secondary accession number(s): Q6FGM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)