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Protein

Vinexin

Gene

SORBS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation by using its third SH3 domain.

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: ProtInc
  • vinculin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120896-MONOMER.
ReactomeiR-HSA-445355. Smooth Muscle Contraction.
SignaLinkiO60504.
SIGNORiO60504.

Names & Taxonomyi

Protein namesi
Recommended name:
Vinexin
Alternative name(s):
SH3-containing adapter molecule 1
Short name:
SCAM-1
Sorbin and SH3 domain-containing protein 3
Gene namesi
Name:SORBS3
Synonyms:SCAM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:30907. SORBS3.

Subcellular locationi

Isoform Alpha :
  • Cell junction By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Localized at cell-extracellular matrix junctions (By similarity). Both isoforms were localized at focal adhesion and cell-cell adhesion sites.By similarity
Isoform Beta :
  • Cell junction By similarity
  • Nucleus
  • Cytoplasmcytoskeleton By similarity

  • Note: Localized at cell-extracellular matrix junctions (By similarity). Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.By similarity

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • focal adhesion Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi649W → F: Loss of SOS-binding ability. 1 Publication1
Mutagenesisi667Y → V: Loss of SOS-binding ability. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000120896.
PharmGKBiPA128394570.

Polymorphism and mutation databases

BioMutaiSORBS3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000658301 – 671VinexinAdd BLAST671
Isoform Beta (identifier: O60504-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei348PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei530Phosphoserine; by MAPK1Combined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei545PhosphoserineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei551PhosphoserineCombined sources1
Modified residuei563PhosphoserineCombined sources1
Isoform Beta (identifier: O60504-2)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei6PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-530 by MAPK1/ERK2 during cell spreading.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60504.
MaxQBiO60504.
PaxDbiO60504.
PeptideAtlasiO60504.
PRIDEiO60504.

PTM databases

iPTMnetiO60504.
PhosphoSitePlusiO60504.

Expressioni

Tissue specificityi

Both isoforms are expressed in different tissues like heart, placenta, brain, skeletal muscle and pancreas. Isoform beta is especially found in liver.

Gene expression databases

BgeeiENSG00000120896.
CleanExiHS_SORBS3.
ExpressionAtlasiO60504. baseline and differential.
GenevisibleiO60504. HS.

Organism-specific databases

HPAiHPA015849.
HPA048034.

Interactioni

Subunit structurei

Interacts with DLG5 through its third SH3 domain (By similarity). Interacts with vinculin by the first two SH3 domains and the proline rich region of vinculin. Binds to SOS (guanine nucleotide exchange factor of RAS and RAC), through its third SH3 domain. The formation of this complex is down-regulated by phosphorylation of SOS. Interacts with INPPL1/SHIP2, SAFB2, SOCS7 and SRCIN1. Interacts with FASLG. Interacts with MAPK1/ERK2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ABL1P005195EBI-1222956,EBI-375543
CPNE2Q96FN45EBI-741237,EBI-7097057
DIP2AQ14689-63EBI-741237,EBI-10233719
EIF3S3Q6IB983EBI-741237,EBI-10184995
HNRNPUL1Q9BUJ23EBI-741237,EBI-1018153
HNRPKQ6IBN13EBI-741237,EBI-3440248
INPPL1O153572EBI-1222953,EBI-1384248
KIAA0408Q6ZU524EBI-741237,EBI-739493
NCKIPSDQ9NZQ33EBI-741237,EBI-745080
PAK2Q131776EBI-741237,EBI-1045887
PAK7Q8TB933EBI-741237,EBI-741896
RARGP136312EBI-1222956,EBI-2568901
RTKNQ9BST93EBI-741237,EBI-446694
SAFB2Q141513EBI-1222956,EBI-352869
SAXO1Q8IYX73EBI-741237,EBI-3957636
SH2D4AQ9H7885EBI-741237,EBI-747035
SH2D4AQ9H788-23EBI-741237,EBI-10308083
Srcin1Q9QWI6-24EBI-1222956,EBI-775607From a different organism.
TBC1D22BQ9NU193EBI-741237,EBI-8787464
TRIM23P364065EBI-741237,EBI-740098
TRIM27P143733EBI-741237,EBI-719493
TRIM41Q8WV443EBI-741237,EBI-725997
VARS2Q5ST30-43EBI-741237,EBI-10244997
VPS37BQ9H9H43EBI-741237,EBI-4400866
VPS37CA5D8V65EBI-741237,EBI-2559305
WASF2Q9Y6W53EBI-1222956,EBI-4290615
WASLO004013EBI-1222956,EBI-957615
ZBTB7BO151563EBI-741237,EBI-740434
ZC4H2Q9NQZ63EBI-741237,EBI-747993

GO - Molecular functioni

  • vinculin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115475. 63 interactors.
IntActiO60504. 143 interactors.
MINTiMINT-262486.
STRINGi9606.ENSP00000240123.

Structurei

Secondary structure

1671
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi385 – 389Combined sources5
Beta strandi395 – 398Combined sources4
Beta strandi406 – 414Combined sources9
Beta strandi417 – 422Combined sources6
Beta strandi425 – 435Combined sources11
Helixi453 – 455Combined sources3
Beta strandi456 – 461Combined sources6
Beta strandi469 – 472Combined sources4
Beta strandi480 – 485Combined sources6
Beta strandi489 – 495Combined sources7
Turni497 – 499Combined sources3
Beta strandi502 – 506Combined sources5
Helixi507 – 509Combined sources3
Beta strandi510 – 514Combined sources5
Beta strandi615 – 621Combined sources7
Beta strandi637 – 644Combined sources8
Beta strandi646 – 648Combined sources3
Beta strandi650 – 657Combined sources8
Beta strandi660 – 663Combined sources4
Turni665 – 667Combined sources3
Beta strandi668 – 670Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CT3NMR-A615-671[»]
2DLMNMR-A383-437[»]
2NWMNMR-A383-438[»]
2YUPNMR-A447-523[»]
ProteinModelPortaliO60504.
SMRiO60504.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60504.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 187SoHoPROSITE-ProRule annotationAdd BLAST73
Domaini380 – 439SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini454 – 515SH3 2PROSITE-ProRule annotationAdd BLAST62
Domaini612 – 671SH3 3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 515Binds to vinculinAdd BLAST136
Regioni612 – 671Binds to SOSAdd BLAST60

Sequence similaritiesi

Contains 3 SH3 domains.PROSITE-ProRule annotation
Contains 1 SoHo domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IPNP. Eukaryota.
ENOG410XP8Q. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000294090.
HOVERGENiHBG054842.
InParanoidiO60504.
OMAiQFHDPAP.
OrthoDBiEOG091G039F.
PhylomeDBiO60504.
TreeFamiTF320680.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR028510. Vinexin.
[Graphical view]
PANTHERiPTHR10663:SF204. PTHR10663:SF204. 1 hit.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: O60504-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGPPRSLRA GLSLDDFIPG HLQSHIGSSS RGTRVPVIRN GGSNTLNFQF
60 70 80 90 100
HDPAPRTVCN GGYTPRRDAS QHPDPAWYQT WPGPGSKPSA STKIPASQHT
110 120 130 140 150
QNWSATWTKD SKRRDKRWVK YEGIGPVDES GMPIAPRSSV DRPRDWYRRM
160 170 180 190 200
FQQIHRKMPD LQLDWTFEEP PRDPRHLGAQ QRPAHRPGPA TSSSGRSWDH
210 220 230 240 250
SEELPRSTFN YRPGAFSTVL QPSNQVLRRR EKVDNVWTEE SWNQFLQELE
260 270 280 290 300
TGQRPKKPLV DDPGEKPSQP IEVLLERELA ELSAELDKDL RAIETRLPSP
310 320 330 340 350
KSSPAPRRAP EQRPPAGPAS AWSSSYPHAP YLGSARSLSP HKMADGGSPF
360 370 380 390 400
LGRRDFVYPS STRDPSASNG GGSPARREEK KRKAARLKFD FQAQSPKELT
410 420 430 440 450
LQKGDIVYIH KEVDKNWLEG EHHGRLGIFP ANYVEVLPAD EIPKPIKPPT
460 470 480 490 500
YQVLEYGEAV AQYTFKGDLE VELSFRKGEH ICLIRKVNEN WYEGRITGTG
510 520 530 540 550
RQGIFPASYV QVSREPRLRL CDDGPQLPTS PRLTAAARSA RHPSSPSALR
560 570 580 590 600
SPADPIDLGG QTSPRRTGFS FPTQEPRPQT QNLGTPGPAL SHSRGPSHPL
610 620 630 640 650
DLGTSSPNTS QIHWTPYRAM YQYRPQNEDE LELREGDRVD VMQQCDDGWF
660 670
VGVSRRTQKF GTFPGNYVAP V
Length:671
Mass (Da):75,341
Last modified:January 11, 2011 - v2
Checksum:i2D8A8718E0367BE9
GO
Isoform Beta (identifier: O60504-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-342: Missing.

Show »
Length:329
Mass (Da):36,742
Checksum:i852B1FDC07859AC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti583L → F in AAD32304 (PubMed:9885244).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057019255P → L.Corresponds to variant rs3758036dbSNPEnsembl.1
Natural variantiVAR_055019556I → T.Combined sources4 PublicationsCorresponds to variant rs2449331dbSNPEnsembl.1
Natural variantiVAR_055020573T → A.Corresponds to variant rs1047030dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0044891 – 342Missing in isoform Beta. 2 PublicationsAdd BLAST342

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064807 mRNA. Translation: AAD32304.1.
AF037261 mRNA. Translation: AAC09244.1.
AC037459 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63675.1.
BC067260 mRNA. Translation: AAH67260.2.
BC091514 mRNA. Translation: AAH91514.1.
BC010146 mRNA. Translation: AAH10146.1.
CCDSiCCDS47824.1. [O60504-2]
CCDS6031.1. [O60504-1]
RefSeqiNP_001018003.1. NM_001018003.2. [O60504-2]
NP_005766.3. NM_005775.4. [O60504-1]
XP_005273428.1. XM_005273371.1. [O60504-2]
XP_016868433.1. XM_017012944.1. [O60504-2]
XP_016868434.1. XM_017012945.1. [O60504-2]
XP_016868435.1. XM_017012946.1. [O60504-2]
UniGeneiHs.528572.

Genome annotation databases

EnsembliENST00000240123; ENSP00000240123; ENSG00000120896. [O60504-1]
ENST00000523965; ENSP00000429764; ENSG00000120896. [O60504-2]
GeneIDi10174.
KEGGihsa:10174.
UCSCiuc003xbv.4. human. [O60504-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064807 mRNA. Translation: AAD32304.1.
AF037261 mRNA. Translation: AAC09244.1.
AC037459 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63675.1.
BC067260 mRNA. Translation: AAH67260.2.
BC091514 mRNA. Translation: AAH91514.1.
BC010146 mRNA. Translation: AAH10146.1.
CCDSiCCDS47824.1. [O60504-2]
CCDS6031.1. [O60504-1]
RefSeqiNP_001018003.1. NM_001018003.2. [O60504-2]
NP_005766.3. NM_005775.4. [O60504-1]
XP_005273428.1. XM_005273371.1. [O60504-2]
XP_016868433.1. XM_017012944.1. [O60504-2]
XP_016868434.1. XM_017012945.1. [O60504-2]
XP_016868435.1. XM_017012946.1. [O60504-2]
UniGeneiHs.528572.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CT3NMR-A615-671[»]
2DLMNMR-A383-437[»]
2NWMNMR-A383-438[»]
2YUPNMR-A447-523[»]
ProteinModelPortaliO60504.
SMRiO60504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115475. 63 interactors.
IntActiO60504. 143 interactors.
MINTiMINT-262486.
STRINGi9606.ENSP00000240123.

PTM databases

iPTMnetiO60504.
PhosphoSitePlusiO60504.

Polymorphism and mutation databases

BioMutaiSORBS3.

Proteomic databases

EPDiO60504.
MaxQBiO60504.
PaxDbiO60504.
PeptideAtlasiO60504.
PRIDEiO60504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240123; ENSP00000240123; ENSG00000120896. [O60504-1]
ENST00000523965; ENSP00000429764; ENSG00000120896. [O60504-2]
GeneIDi10174.
KEGGihsa:10174.
UCSCiuc003xbv.4. human. [O60504-1]

Organism-specific databases

CTDi10174.
GeneCardsiSORBS3.
H-InvDBHIX0201306.
HGNCiHGNC:30907. SORBS3.
HPAiHPA015849.
HPA048034.
MIMi610795. gene.
neXtProtiNX_O60504.
OpenTargetsiENSG00000120896.
PharmGKBiPA128394570.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPNP. Eukaryota.
ENOG410XP8Q. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000294090.
HOVERGENiHBG054842.
InParanoidiO60504.
OMAiQFHDPAP.
OrthoDBiEOG091G039F.
PhylomeDBiO60504.
TreeFamiTF320680.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120896-MONOMER.
ReactomeiR-HSA-445355. Smooth Muscle Contraction.
SignaLinkiO60504.
SIGNORiO60504.

Miscellaneous databases

ChiTaRSiSORBS3. human.
EvolutionaryTraceiO60504.
GeneWikiiSORBS3.
GenomeRNAii10174.
PROiO60504.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120896.
CleanExiHS_SORBS3.
ExpressionAtlasiO60504. baseline and differential.
GenevisibleiO60504. HS.

Family and domain databases

InterProiIPR001452. SH3_domain.
IPR003127. SoHo_dom.
IPR028510. Vinexin.
[Graphical view]
PANTHERiPTHR10663:SF204. PTHR10663:SF204. 1 hit.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
PF02208. Sorb. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
SM00459. Sorb. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS50831. SOHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVINEX_HUMAN
AccessioniPrimary (citable) accession number: O60504
Secondary accession number(s): Q5BJE4
, Q6NX54, Q96FY4, Q9UQE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 171 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.