O60499 (STX10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-10
Short name=Syn10

Gene names

Name:	STX10
Synonyms:	SYN10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Ref.6
Subunit structure	Interacts with VPS52. Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein Potential.
Tissue specificity	Expressed at high levels in heart, skeletal muscle and pancreas.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi vesicle transport Inferred from electronic annotation. Source: InterPro intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, endosome to Golgi Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	Golgi membrane Traceable author statement Ref.1. Source: ProtInc integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60499-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60499-2)

The sequence of this isoform differs from the canonical sequence as follows:
     76-124: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 249

249

Syntaxin-10

PRO_0000210220

Regions

Topological domain

1 – 228

228

Cytoplasmic Potential

Transmembrane

229 – 249

21

Helical; Anchor for type IV membrane protein; Potential

Domain

157 – 219

63

t-SNARE coiled-coil homology

Coiled coil

41 – 69

29

Potential

Amino acid modifications

Modified residue

108

1

Phosphoserine Ref.10

Modified residue

110

1

Phosphothreonine Ref.10

Modified residue

134

1

Phosphoserine Ref.9

Modified residue

140

1

Phosphoserine Ref.8 Ref.9

Modified residue

143

1

Phosphoserine Ref.8 Ref.9

Natural variations

Alternative sequence

76 – 124

49

Missing in isoform 2.

VSP_006347

Secondary structure

1

.

.

.

.

.

.

.

.

.

249

Helix Strand Turn

Details...

Helix

7 – 30

24

Helix

42 – 73

32

Helix

75 – 78

4

Helix

82 – 106

25

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 795AB3055F25AD5F	FASTA	249	28,114
	10 20 30 40 50 60 MSLEDPFFVV RGEVQKAVNT ARGLYQRWCE LLQESAAVGR EELDWTTNEL RNGLRSIEWD 70 80 90 100 110 120 LEDLEETIGI VEANPGKFKL PAGDLQERKV FVERMREAVQ EMKDHMVSPT AVAFLERNNR 130 140 150 160 170 180 EILAGKPAAQ KSPSDLLDAS AVSATSRYIE EQQATQQLIM DEQDQQLEMV SGSIQVLKHM 190 200 210 220 230 240 SGRVGEELDE QGIMLDAFAQ EMDHTQSRMD GVLRKLAKVS HMTSDRRQWC AIAVLVGVLL LVLILLFSL
	Isoform 2 [UniParc]. Checksum: C196F05CCAD78966	FASTA	200	22,445
	10 20 30 40 50 60 MSLEDPFFVV RGEVQKAVNT ARGLYQRWCE LLQESAAVGR EELDWTTNEL RNGLRSIEWD 70 80 90 100 110 120 LEDLEETIGI VEANPGKPAA QKSPSDLLDA SAVSATSRYI EEQQATQQLI MDEQDQQLEM 130 140 150 160 170 180 VSGSIQVLKH MSGRVGEELD EQGIMLDAFA QEMDHTQSRM DGVLRKLAKV SHMTSDRRQW 190 200 CAIAVLVGVL LLVLILLFSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Syntaxin 10: a member of the syntaxin family localized to the trans-Golgi network." Tang B.L., Low D.Y.H., Tan A.E.H., Hong W. Biochem. Biophys. Res. Commun. 242:345-350(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[5]	"Characterization of the human GARP (Golgi associated retrograde protein) complex." Liewen H., Meinhold-Heerlein I., Oliveira V., Schwarzenbacher R., Luo G., Wadle A., Jung M., Pfreundschuh M., Stenner-Liewen F. Exp. Cell Res. 306:24-34(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VSP52.
[6]	"A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells." Ganley I.G., Espinosa E., Pfeffer S.R. J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140 AND SER-143, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134; SER-140 AND SER-143, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108 AND THR-110, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF035531 mRNA. Translation: AAC05087.1.
CR457110 mRNA. Translation: CAG33391.1.
AC011446 Genomic DNA. No translation available.
BC017237 mRNA. Translation: AAH17237.1.

IPI

IPI00178839.
IPI00293402.

PIR

JC5922.

RefSeq

NP_001258540.1. NM_001271611.1.
NP_003756.1. NM_003765.2.

UniGene

Hs.43812.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4DND	X-ray	1.40	A	1-108	[»]

ProteinModelPortal

O60499.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000242770.

PTM databases

PhosphoSite

O60499.

Proteomic databases

PaxDb

O60499.

PRIDE

O60499.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000343587; ENSP00000339350; ENSG00000104915.
ENST00000587230; ENSP00000466298; ENSG00000104915.

GeneID

8677.

KEGG

hsa:8677.

UCSC

uc021upq.1. human.

Organism-specific databases

CTD

8677.

GeneCards

GC19M013254.

HGNC

HGNC:11428. STX10.

HPA

HPA035303.

MIM

603765. gene.

neXtProt

NX_O60499.

PharmGKB

PA36228.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG46901.

HOGENOM

HOG000237350.

HOVERGEN

HBG007194.

InParanoid

O60499.

KO

K08498.

OMA

HMVSPTA.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_13685. Neuronal System.

Gene expression databases

ArrayExpress

O60499.

Bgee

O60499.

CleanEx

HS_STX10.

Genevestigator

O60499.

GermOnline

ENSG00000104915. Homo sapiens.

Family and domain databases

InterPro

IPR015260. Syntaxin-6_N.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
PF09177. Syntaxin-6_N. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

8677.

NextBio

32551.

PMAP-CutDB

O60499.

SOURCE

Search...

Entry information

Entry name

STX10_HUMAN

Accession

Primary (citable) accession number: O60499
Secondary accession number(s): A6NC41, Q6IAP4, Q96AE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.