Reviewed, UniProtKB/Swiss-Prot O60476 (MA1A2_HUMAN)

Names and origin

Protein names

Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
    EC=3.2.1.113
Alternative name(s):
    Processing alpha-1,2-mannosidase IB
      Short name=Alpha-1,2-mannosidase IB
    Mannosidase alpha class 1A member 2

Gene names

Name:	MAN1A2
Synonyms:	MAN1B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Calcium.
Enzyme regulation	Inhibited by both 1-deoxymannojirimycin and kifunensine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Highest levels of expression in placenta and testis.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-glycan processing Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB		PRO_0000210312
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Signal-anchor for type II membrane protein Potential
	Topological domain	58 – 641	584	Lumenal Potential
Amino acid modifications
	Glycosylation	631	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O60476-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 19DA691A9C4B0ED3	FASTA	641	73,004
	10 20 30 40 50 60 MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC FGAFFFLPDS 70 80 90 100 110 120 SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH REEEERLRNK IRADHEKALE 130 140 150 160 170 180 EAKEKLRKSR EEIRAEIQTE KNKVVQEMKI KENKPLPPVP IPNLVGIRGG DPEDNDIREK 190 200 210 220 230 240 REKIKEMMKH AWDNYRTYGW GHNELRPIAR KGHSPNIFGS SQMGATIVDA LDTLYIMGLH 250 260 270 280 290 300 DEFLDGQRWI EDNLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKIK AVQLAEKLLP 310 320 330 340 350 360 AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFIHLSYLT GDLTYYKKVM 370 380 390 400 410 420 HIRKLLQKMD RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS FYEYLLKAWL MSDKTDHEAR 430 440 450 460 470 480 KMYDDAIEAI EKHLIKKSRG GLTFIGEWKN GHLEKKMGHL ACFAGGMFAL GADGSRADKA 490 500 510 520 530 540 GHYLELGAEI ARTCHESYDR TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY 550 560 570 580 590 600 LWRFTHDPRY RQWGWEAALA IEKYCRVNGG FSGVKDVYSS TPTHDDVQQS FFLAETLKYL 610 620 630 640 YLLFSGDDLL PLDHWVFNTE AHPLPVLHLA NTTLSGNPAV R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha-1,2-mannosidase gene involved in N-glycan maturation." Tremblay L.O., Campbell-Dyke N., Herscovics A. Glycobiology 8:585-595(1998) [PubMed: 9592125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain and Placenta.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF053626 , AF053622, AF053623, AF053624, AF053625 Genomic DNA. Translation: AAC26201.1. AF027156 mRNA. Translation: AAC26169.1. AF053621 , AF053619, AF053620, AF053615, AF053616, AF053617, AF053618 Genomic DNA. Translation: AAC26200.1. AL157902, AL358072 Genomic DNA. Translation: CAI22315.1. AL358072, AL157902 Genomic DNA. Translation: CAH71079.1. BC063300 mRNA. Translation: AAH63300.1.
IPI	IPI00009145.
RefSeq	NP_006690.1.
UniGene	Hs.435938
3D structure databases
HSSP	HSSP built from PDB template 1DL2 based on UniProtKB P32906.
ModBase	Search...
Protein-protein interaction databases
STRING	O60476.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Proteomic databases
PRIDE	O60476.
Genome annotation databases
Ensembl	ENST00000329466; ENSP00000358462; ENSG00000198162; Homo sapiens. [Genome view] ENST00000356554; ENSP00000348959; ENSG00000198162; Homo sapiens. [Genome view] ENST00000369450; ENSP00000358461; ENSG00000198162; Homo sapiens. [Genome view] ENST00000413355; ENSP00000399773; ENSG00000198162; Homo sapiens. [Genome view] ENST00000421535; ENSP00000396362; ENSG00000198162; Homo sapiens. [Genome view] ENST00000422329; ENSP00000402416; ENSG00000198162; Homo sapiens. [Genome view] ENST00000449370; ENSP00000412706; ENSG00000198162; Homo sapiens. [Genome view]
GeneID	10905.
KEGG	hsa:10905.
UCSC	uc001ehd.1. human.
Organism-specific databases
CTD	10905.
GeneCards	GC01P117623.
H-InvDB	HIX0000937.
HGNC	HGNC:6822. MAN1A2.
MIM	604345. gene.
PharmGKB	PA30571.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60476.
HOVERGEN	O60476.
OMA	DHEARKM.
Enzyme and pathway databases
BRENDA	3.2.1.113. 247.
Gene expression databases
ArrayExpress	O60476.
Bgee	O60476.
CleanEx	HS_MAN1A2.
Genevestigator	O60476.
GermOnline	ENSG00000198162. Homo sapiens.
Family and domain databases
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
Gene3D	G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
PANTHER	PTHR11742. Glyco_hydro_47. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
ProtoNet	Search...
Other Resources
NextBio	41419.
SOURCE	Search...

Entry information

Entry name

MA1A2_HUMAN

Accession

Primary (citable) accession number: O60476
Secondary accession number(s): Q9H510

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.