Reviewed,
UniProtKB/Swiss-Prot O60437 (PEPL_HUMAN)
Last modified
November 25, 2008.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Periplakin Alternative name(s): 195 kDa cornified envelope precursor protein 190 kDa paraneoplastic pemphigus antigen | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1756 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. |
| Subunit structure | Homodimer or a heterodimer with EVPL. Interacts with PPHLN1 and VIM. Binds to the PH domain of AKT1. Interacts with FCGR1A. |
| Subcellular location | Cell junction › desmosome. Cytoplasm › cytoskeleton. Cell membraneBy similarity. NucleusBy similarity. MitochondrionBy similarity. Note= Associated with desmosomes and intermediate filaments. |
| Tissue specificity | Expressed in stratified squamous epithelia and in some other epithelia. |
| Induction | During differentiation of epidermal keratinocytes. |
| Sequence similarities | Belongs to the plakin or cytolinker family. Contains 2 plectin repeats. Contains 4 spectrin repeats. |
Ontologies
Keywords | |
|---|---|
| Biological process | Keratinization |
| Cellular component | Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Mitochondrion Nucleus |
| Domain | Coiled coil Repeat |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | keratinization Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Ref.2Traceable author statement. Source: ProtInc mitochondrionInferred from electronic annotation. Source: UniProtKB-KW nucleusInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | protein binding Ref.5 Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Ref.2Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1756 | 1756 | Periplakin | PRO_0000078149 | |||||
Regions | |||||||||
| Repeat | 216 – 317 | 102 | Spectrin 1 | ||||||
| Repeat | 323 – 485 | 163 | Spectrin 2 | ||||||
| Repeat | 505 – 612 | 108 | Spectrin 3 | ||||||
| Repeat | 733 – 861 | 129 | Spectrin 4 | ||||||
| Repeat | 1651 – 1685 | 35 | Plectin 1 | ||||||
| Repeat | 1700 – 1735 | 36 | Plectin 2 | ||||||
| Coiled coil | 16 – 125 | 110 | Potential | ||||||
| Coiled coil | 188 – 389 | 202 | Potential | ||||||
| Coiled coil | 585 – 820 | 236 | Potential | ||||||
| Coiled coil | 886 – 1645 | 760 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1331 | 1 | Phosphoserine | ||||||
| Modified residue | 1579 | 1 | Phosphothreonine | ||||||
Experimental info | |||||||||
| Sequence conflict | 589 | 1 | Q → R Ref.2 Ref.3 | ||||||
| Sequence conflict | 657 | 1 | A → P Ref.2 Ref.3 | ||||||
| Sequence conflict | 994 | 1 | V → F in AAC17738. Ref.1 | ||||||
| Sequence conflict | 1573 | 1 | Q → E in BAA25494. Ref.4 | ||||||
| Sequence conflict | 1663 | 1 | P → L in AAC17738. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Periplakin, a novel component of cornified envelopes and desmosomes that belongs to the plakin family and forms complexes with envoplakin." Ruhrberg C., Hajibagheri M.A.N., Parry D.A.D., Watt F.M. J. Cell Biol. 139:1835-1849(1997) [PubMed: 9412476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH EVPL. Tissue: Keratinocyte. |
| [2] | "cDNA cloning, mRNA expression, and chromosomal mapping of human and mouse periplakin genes." Aho S., McLean W.H.I., Li K., Uitto J. Genomics 48:242-247(1998) [PubMed: 9521878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Keratinocyte. |
| [3] | "Human periplakin: genomic organization in a clonally unstable region of chromosome 16p with an abundance of repetitive sequence elements." Aho S., Rothenberger K., Tan E.M.L., Ryoo Y.W., Cho B.H., McLean W.H.I., Uitto J. Genomics 56:160-168(1999) [PubMed: 10051401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 331-1756. Tissue: Brain. |
| [5] | "Binding of protein kinase B to the plakin family member periplakin." van den Heuvel A.P., de Vries-Smits A.M.M., van Weeren P.C., Dijkers P.F., de Bruyn K.M., Riedl J.A., Burgering B.M.T. J. Cell Sci. 115:3957-3966(2002) [PubMed: 12244133] [Abstract] Cited for: INTERACTION WITH AKT1. Tissue: Embryo. |
| [6] | "Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity." Beekman J.M., Bakema J.E., van de Winkel J.G.J., Leusen J.H.W. Proc. Natl. Acad. Sci. U.S.A. 101:10392-10397(2004) [PubMed: 15229321] [Abstract] Cited for: INTERACTION WITH FCGR1A. |
| [7] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1331, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1579, MASS SPECTROMETRY. Tissue: Epithelium. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF001691 mRNA. Translation: AAC17738.1. AF013717 mRNA. Translation: AAC39668.1. AF041004 AF041003 Genomic DNA. Translation: AAD17459.1. AB011140 mRNA. Translation: BAA25494.1. | |
| PIR | T00337. |
| RefSeq | NP_002696.3. |
| UniGene | Hs.192233 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QSD based on UniProtKB P48606. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O60437. |
PTM databases | |
| PhosphoSite | O60437. |
Genome annotation databases | |
| Ensembl | ENSG00000118898. Homo sapiens. [Contig view] |
| GeneID | 5493. |
| KEGG | hsa:5493. |
Organism-specific databases | |
| H-InvDB | HIX0012794. |
| HGNC | HGNC:9273. PPL. |
| MIM | 602871. gene. |
| PharmGKB | PA142671174. |
| HUGE | Search... |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | O60437. |
| HOVERGEN | O60437. |
Gene expression databases | |
| CleanEx | HS_PPL. |
| GermOnline | ENSG00000118898. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001101. Plectin_repeat. IPR002017. Spectrin_repeat. [Graphical view] |
| Pfam | PF00681. Plectin. 1 hit. [Graphical view] |
| SMART | SM00250. PLEC. 2 hits. SM00150. SPEC. 5 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 21238. |
| SOURCE | Search... |
Entry information
| Entry name | PEPL_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O60437 Secondary accession number(s): O60314, O60454 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


