Reviewed, UniProtKB/Swiss-Prot O60423 (AT8B3_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable phospholipid-transporting ATPase IK
    EC=3.6.3.1
Alternative name(s):
    ATPase class I type 8B member 3

Gene names

Name:	ATP8B3
Synonyms:	ATP1K, FOS37502_2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.
Sequence caution	The sequence AAC17601.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60423-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: O60423-2)

The sequence of this isoform differs from the canonical sequence as follows:
     731-740: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1310	1310	Probable phospholipid-transporting ATPase IK		PRO_0000046367
Regions
	Topological domain	1 – 149	149	Cytoplasmic Potential
	Transmembrane	150 – 171	22	Potential
	Topological domain	172 – 177	6	Extracellular Potential
	Transmembrane	178 – 197	20	Potential
	Topological domain	198 – 381	184	Cytoplasmic Potential
	Transmembrane	382 – 403	22	Potential
	Topological domain	404 – 430	27	Extracellular Potential
	Transmembrane	431 – 452	22	Potential
	Topological domain	453 – 1005	553	Cytoplasmic Potential
	Transmembrane	1006 – 1026	21	Potential
	Topological domain	1027 – 1038	12	Extracellular Potential
	Transmembrane	1039 – 1058	20	Potential
	Topological domain	1059 – 1088	30	Cytoplasmic Potential
	Transmembrane	1089 – 1110	22	Potential
	Topological domain	1111 – 1122	12	Extracellular Potential
	Transmembrane	1123 – 1145	23	Potential
	Topological domain	1146 – 1151	6	Cytoplasmic Potential
	Transmembrane	1152 – 1172	21	Potential
	Topological domain	1173 – 1192	20	Extracellular Potential
	Transmembrane	1193 – 1217	25	Potential
	Topological domain	1218 – 1310	93	Cytoplasmic Potential
Sites
	Active site	495	1	4-aspartylphosphate intermediate By similarity
	Metal binding	949	1	Magnesium By similarity
	Metal binding	953	1	Magnesium By similarity
Natural variations
	Alternative sequence	731 – 740	10	Missing in isoform 2.		VSP_007304
	Natural variant	45	1	G → R: dbSNP rs7250872.		VAR_055544
	Natural variant	618	1	V → I: dbSNP rs8100856.		VAR_055545

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 30, 2003. Version 3. Checksum: F1A1C25A8DE696FC	FASTA	1,310	148,031
	10 20 30 40 50 60 MGTGPAQTPR STRAGPEPSP APPGPGDTGD SDVTQEGSGP AGIRGGETVI RAGMGDSPGR 70 80 90 100 110 120 GAPERRHKAQ PGRARKYEWR PEGPTSMGSL GQREDLQDED RNSAFTWKVQ ANNRAYNGQF 130 140 150 160 170 180 KEKVILCWQR KKYKTNVIRT AKYNFYSFLP LNLYEQFHRV SNLFFLIIII LQSIPDISTL 190 200 210 220 230 240 PWFSLSTPMV CLLFIRATRD LVDDMGRHKS DRAINNRPCQ ILMGKSFKQK KWQDLCVGDV 250 260 270 280 290 300 VCLRKDNIVP ADMLLLASTE PSSLCYVETV DIDGETNLKF RQALMVTHKE LATIKKMASF 310 320 330 340 350 360 QGTVTCEAPN SRMHHFVGCL EWNDKKYSLD IGNLLLRGCR IRNTDTCYGL VIYAGFDTKI 370 380 390 400 410 420 MKNCGKIHLK RTKLDLLMNK LVVVIFISVV LVCLVLAFGF GFSVKEFKDH HYYLSGVHGS 430 440 450 460 470 480 SVAAESFFVF WSFLILLSVT IPMSMFILSE FIYLGNSVFI DWDVQMYYKP QDVPAKARST 490 500 510 520 530 540 SLNDHLGQVE YIFSDKTGTL TQNILTFNKC CISGRVYGPD SEATTRPKEN PYLWNKFADG 550 560 570 580 590 600 KLLFHNAALL HLVRTNGDEA VREFWRLLAI CHTVMVRESP RERPDQLLYQ AASPDEGALV 610 620 630 640 650 660 TAARNFGYVF LSRTQDTVTI MELGEERVYQ VLAIMDFNST RKRMSVLVRK PEGAICLYTK 670 680 690 700 710 720 GADTVIFERL HRRGAMEFAT EEALAAFAQE TLRTLCLAYR EVAEDIYEDW QQRHQEASLL 730 740 750 760 770 780 LQNRAQALQQ VYNEMEQDLR LLGATAIEDR LQDGVPETIK CLKKSNIKIW VLTGDKQETA 790 800 810 820 830 840 VNIGFACELL SENMLILEEK EISRILETYW ENSNNLLTRE SLSQVKLALV INGDFLDKLL 850 860 870 880 890 900 VSLRKEPRAL AQNVNMDEAW QELGQSRRDF LYARRLSLLC RRFGLPLAAP PAQDSRARRS 910 920 930 940 950 960 SEVLQERAFV DLASKCQAVI CCRVTPKQKA LIVALVKKYH QVVTLAIGDG ANDINMIKTA 970 980 990 1000 1010 1020 DVGVGLAGQE GMQAVQNSDF VLGQFCFLQR LLLVHGRWSY VRICKFLRYF FYKSMASMMV 1030 1040 1050 1060 1070 1080 QVWFACYNGF TGQPLYEGWF LALFNLLYST LPVLYIGLFE QDVSAEQSLE KPELYVVGQK 1090 1100 1110 1120 1130 1140 DELFNYWVFV QAIAHGVTTS LVNFFMTLWI SRDTAGPASF SDHQSFAVVV ALSCLLSITM 1150 1160 1170 1180 1190 1200 EVILIIKYWT ALCVATILLS LGFYAIMTTT TQSFWLFRVS PTTFPFLYAD LSVMSSPSIL 1210 1220 1230 1240 1250 1260 LVVLLSVSIN TFPVLALRVI FPALKELRAK EEKVEEGPSE EIFTMEPLPH VHRESRARRS 1270 1280 1290 1300 1310 SYAFSHREGY ANLITQGTIL RRGPGVSSDI ASESLDPSDE EAASSPKESQ
	Isoform 2. Checksum: 356B1972DD49DFF5	FASTA	1,300	146,752
	10 20 30 40 50 60 MGTGPAQTPR STRAGPEPSP APPGPGDTGD SDVTQEGSGP AGIRGGETVI RAGMGDSPGR 70 80 90 100 110 120 GAPERRHKAQ PGRARKYEWR PEGPTSMGSL GQREDLQDED RNSAFTWKVQ ANNRAYNGQF 130 140 150 160 170 180 KEKVILCWQR KKYKTNVIRT AKYNFYSFLP LNLYEQFHRV SNLFFLIIII LQSIPDISTL 190 200 210 220 230 240 PWFSLSTPMV CLLFIRATRD LVDDMGRHKS DRAINNRPCQ ILMGKSFKQK KWQDLCVGDV 250 260 270 280 290 300 VCLRKDNIVP ADMLLLASTE PSSLCYVETV DIDGETNLKF RQALMVTHKE LATIKKMASF 310 320 330 340 350 360 QGTVTCEAPN SRMHHFVGCL EWNDKKYSLD IGNLLLRGCR IRNTDTCYGL VIYAGFDTKI 370 380 390 400 410 420 MKNCGKIHLK RTKLDLLMNK LVVVIFISVV LVCLVLAFGF GFSVKEFKDH HYYLSGVHGS 430 440 450 460 470 480 SVAAESFFVF WSFLILLSVT IPMSMFILSE FIYLGNSVFI DWDVQMYYKP QDVPAKARST 490 500 510 520 530 540 SLNDHLGQVE YIFSDKTGTL TQNILTFNKC CISGRVYGPD SEATTRPKEN PYLWNKFADG 550 560 570 580 590 600 KLLFHNAALL HLVRTNGDEA VREFWRLLAI CHTVMVRESP RERPDQLLYQ AASPDEGALV 610 620 630 640 650 660 TAARNFGYVF LSRTQDTVTI MELGEERVYQ VLAIMDFNST RKRMSVLVRK PEGAICLYTK 670 680 690 700 710 720 GADTVIFERL HRRGAMEFAT EEALAAFAQE TLRTLCLAYR EVAEDIYEDW QQRHQEASLL 730 740 750 760 770 780 LQNRAQALQQ LLGATAIEDR LQDGVPETIK CLKKSNIKIW VLTGDKQETA VNIGFACELL 790 800 810 820 830 840 SENMLILEEK EISRILETYW ENSNNLLTRE SLSQVKLALV INGDFLDKLL VSLRKEPRAL 850 860 870 880 890 900 AQNVNMDEAW QELGQSRRDF LYARRLSLLC RRFGLPLAAP PAQDSRARRS SEVLQERAFV 910 920 930 940 950 960 DLASKCQAVI CCRVTPKQKA LIVALVKKYH QVVTLAIGDG ANDINMIKTA DVGVGLAGQE 970 980 990 1000 1010 1020 GMQAVQNSDF VLGQFCFLQR LLLVHGRWSY VRICKFLRYF FYKSMASMMV QVWFACYNGF 1030 1040 1050 1060 1070 1080 TGQPLYEGWF LALFNLLYST LPVLYIGLFE QDVSAEQSLE KPELYVVGQK DELFNYWVFV 1090 1100 1110 1120 1130 1140 QAIAHGVTTS LVNFFMTLWI SRDTAGPASF SDHQSFAVVV ALSCLLSITM EVILIIKYWT 1150 1160 1170 1180 1190 1200 ALCVATILLS LGFYAIMTTT TQSFWLFRVS PTTFPFLYAD LSVMSSPSIL LVVLLSVSIN 1210 1220 1230 1240 1250 1260 TFPVLALRVI FPALKELRAK EEKVEEGPSE EIFTMEPLPH VHRESRARRS SYAFSHREGY 1270 1280 1290 1300 ANLITQGTIL RRGPGVSSDI ASESLDPSDE EAASSPKESQ

References

[1]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Eye and Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 978-1310. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC004755 Genomic DNA. Translation: AAC17601.1. Sequence problems. AC012615 Genomic DNA. No translation available. AK057452 mRNA. Translation: BAB71492.1. Different initiation. BC035162 mRNA. Translation: AAH35162.3.
IPI	IPI00216974. IPI00237913.
RefSeq	NP_620168.1.
UniGene	Hs.306212
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O60423.
Proteomic databases
PRIDE	O60423.
Genome annotation databases
Ensembl	ENST00000310127; ENSP00000311336; ENSG00000130270; Homo sapiens. [Genome view] ENST00000382339; ENSP00000371776; ENSG00000130270; Homo sapiens. [Genome view]
GeneID	148229.
KEGG	hsa:148229.
UCSC	uc002ltw.1. human.
Organism-specific databases
CTD	148229.
GeneCards	GC19M001733.
HGNC	HGNC:13535. ATP8B3.
MIM	605866. gene.
PharmGKB	PA25168.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60423.
HOVERGEN	O60423.
OMA	RVYQVLA.
Enzyme and pathway databases
BRENDA	3.6.3.1. 247.
Gene expression databases
ArrayExpress	O60423.
Bgee	O60423.
CleanEx	HS_ATP8B3.
Genevestigator	O60423.
GermOnline	ENSG00000130270. Homo sapiens.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	85875.
SOURCE	Search...

Entry information

Entry name

AT8B3_HUMAN

Accession

Primary (citable) accession number: O60423
Secondary accession number(s): Q8IVB8, Q8N4Y8, Q96M22

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 30, 2003
Last modified:	November 3, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.