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O60381 (HBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HMG box-containing protein 1
Alternative name(s):
HMG box transcription factor 1
High mobility group box transcription factor 1
Gene names
Name:HBP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4. Ref.1 Ref.6 Ref.7

Subunit structure

Binds the second PAH repeat of SIN3A By similarity. Binds TCF4 and RB1.

Subcellular location

Nucleus Ref.6.

Sequence similarities

Contains 1 AXH domain.

Contains 1 HMG box DNA-binding domain.

Sequence caution

The sequence BAB85059.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RB1P064002EBI-954175,EBI-491274

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60381-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60381-2)

The sequence of this isoform differs from the canonical sequence as follows:
     463-514: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: O60381-3)

The sequence of this isoform differs from the canonical sequence as follows:
     510-514: GSQQH → VCFNK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514HMG box-containing protein 1
PRO_0000048546

Regions

Domain203 – 345143AXH
DNA binding434 – 50269HMG box
Compositional bias156 – 1616Poly-Ser
Compositional bias197 – 2004Poly-Asp

Natural variations

Alternative sequence463 – 51452Missing in isoform 2.
VSP_014655
Alternative sequence510 – 5145GSQQH → VCFNK in isoform 3.
VSP_014656

Experimental info

Sequence conflict1271Q → L in AAH22329. Ref.5
Sequence conflict3481S → P in AAH22329. Ref.5

Secondary structure

............................ 514
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 2AFADC995F73A031

FASTA51457,645
        10         20         30         40         50         60 
MVWEVKTNQM PNAVQKLLLV MDKRASGMND SLELLQCNEN LPSSPGYNSC DEHMELDDLP 

        70         80         90        100        110        120 
ELQAVQSDPT QSGMYQLSSD VSHQEYPRSS WNQNTSDIPE TTYRENEVDW LTELANIATS 

       130        140        150        160        170        180 
PQSPLMQCSF YNRSSPVHII ATSKSLHSYA RPPPVSSSSK SEPAFPHHHW KEETPVRHER 

       190        200        210        220        230        240 
ANSESESGIF CMSSLSDDDD LGWCNSWPST VWHCFLKGTR LCFHKGSNKE WQDVEDFARA 

       250        260        270        280        290        300 
EGCDNEEDLQ MGIHKGYGSD GLKLLSHEES VSFGESVLKL TFDPGTVEDG LLTVECKLDH 

       310        320        330        340        350        360 
PFYVKNKGWS SFYPSLTVVQ HGIPCCEVHI GDVCLPPGHP DAINFDDSGV FDTFKSYDFT 

       370        380        390        400        410        420 
PMDSSAVYVL SSMARQRRAS LSCGGPGGQD FARSGFSKNC GSPGSSQLSS NSLYAKAVKN 

       430        440        450        460        470        480 
HSSGTVSATS PNKCKRPMNA FMLFAKKYRV EYTQMYPGKD NRAISVILGD RWKKMKNEER 

       490        500        510 
RMYTLEAKAL AEEQKRLNPD CWKRKRTNSG SQQH 

« Hide

Isoform 2 [UniParc].

Checksum: F2972041A89482BC
Show »

FASTA46251,433
Isoform 3 [UniParc].

Checksum: 2838539245C3A031
Show »

FASTA51457,699

References

« Hide 'large scale' references
[1]"Involvement of retinoblastoma protein and HBP1 in histone H1(0) gene expression."
Lemercier C., Duncliffe K., Boibessot I., Zhang H., Verdel A., Angelov D., Khochbin S.
Mol. Cell. Biol. 20:6627-6637(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH RB1.
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-514 (ISOFORM 2).
Tissue: Hepatoma and Testis.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Uterus.
[6]"Human HMG box transcription factor HBP1: a role in hCD2 LCR function."
Zhuma T., Tyrrell R., Sekkali B., Skavdis G., Saveliev A., Tolaini M., Roderick K., Norton T., Smerdon S., Sedgwick S., Festenstein R., Kioussis D.
EMBO J. 18:6396-6406(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Negative regulation of the Wnt-beta-catenin pathway by the transcriptional repressor HBP1."
Sampson E.M., Haque Z.K., Ku M.-C., Tevosian S.G., Albanese C., Pestell R.G., Paulson K.E., Yee A.S.
EMBO J. 20:4500-4511(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TCF4.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Solution structure of the HMG box domain from human HMG-box transcription factor 1."
RIKEN structural genomics initiative (RSGI)
Submitted (JUL-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 422-503.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF182038 mRNA. Translation: AAD56225.1.
AK074353 mRNA. Translation: BAB85059.1. Different initiation.
AK122785 mRNA. Translation: BAG53729.1.
AC004492 Genomic DNA. Translation: AAC08317.1.
CH471070 Genomic DNA. Translation: EAW83392.1.
BC017069 mRNA. Translation: AAH17069.1.
BC022329 mRNA. Translation: AAH22329.1.
CCDSCCDS5741.1. [O60381-1]
RefSeqNP_001231191.1. NM_001244262.1.
NP_036389.2. NM_012257.3. [O60381-1]
XP_005250323.1. XM_005250266.2. [O60381-1]
XP_005250324.1. XM_005250267.2. [O60381-1]
UniGeneHs.162032.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E6ONMR-A424-503[»]
3QVEX-ray2.04A/B/C206-342[»]
ProteinModelPortalO60381.
SMRO60381. Positions 208-345, 425-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117927. 17 interactions.
IntActO60381. 7 interactions.
MINTMINT-1185770.
STRING9606.ENSP00000222574.

PTM databases

PhosphoSiteO60381.

Proteomic databases

MaxQBO60381.
PaxDbO60381.
PRIDEO60381.

Protocols and materials databases

DNASU26959.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222574; ENSP00000222574; ENSG00000105856. [O60381-1]
ENST00000468410; ENSP00000420500; ENSG00000105856. [O60381-1]
ENST00000485846; ENSP00000418738; ENSG00000105856. [O60381-1]
GeneID26959.
KEGGhsa:26959.
UCSCuc003vdy.3. human. [O60381-1]
uc003veb.1. human. [O60381-3]

Organism-specific databases

CTD26959.
GeneCardsGC07P106809.
HGNCHGNC:23200. HBP1.
HPACAB032824.
HPA020225.
neXtProtNX_O60381.
PharmGKBPA134901346.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149693.
HOVERGENHBG052810.
InParanoidO60381.
OMACCEIHIG.
OrthoDBEOG7VX8VK.
PhylomeDBO60381.
TreeFamTF105381.

Gene expression databases

ArrayExpressO60381.
BgeeO60381.
CleanExHS_HBP1.
GenevestigatorO60381.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR013723. Ataxin-1_HBP1.
IPR003652. Ataxin_AXH_dom.
IPR009071. HMG_box_dom.
[Graphical view]
PfamPF08517. AXH. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00536. AXH. 1 hit.
SM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF102031. SSF102031. 1 hit.
SSF47095. SSF47095. 1 hit.
PROSITEPS51148. AXH. 1 hit.
PS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHBP1. human.
EvolutionaryTraceO60381.
GeneWikiHBP1.
GenomeRNAi26959.
NextBio49416.
PROO60381.

Entry information

Entry nameHBP1_HUMAN
AccessionPrimary (citable) accession number: O60381
Secondary accession number(s): B3KVB7 expand/collapse secondary AC list , Q8TBM1, Q8TE93, Q96AJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM