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Protein

Nuclear protein 1

Gene

NUPR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chromatin-binding protein that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Interacts with MSL1 and inhibits its activity on histone H4 'Lys-16' acetylation (H4K16ac). Binds the RELB promoter and activates its transcription, leading to the transactivation of IER3. The NUPR1/RELB/IER3 survival pathway may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. In breast cancer cells, NUPR1 overexpression leads to the activation of PI3K/AKT signaling pathway, CDKN1A/p21 phosphorylation and relocalization from the nucleus to the cytoplasm, leading to resistance to chemotherapeutic agents, such as doxorubicin.3 Publications

GO - Molecular functioni

  • chromatin binding Source: GO_Central
  • DNA binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiO60356

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein 1Curated
Alternative name(s):
Candidate of metastasis 1
Nuclear transcriptional regulator protein 1Imported
Protein p8
Gene namesi
Name:NUPR1Imported
Synonyms:COM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000176046.8
HGNCiHGNC:29990 NUPR1
MIMi614812 gene
neXtProtiNX_O60356

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26471
OpenTargetsiENSG00000176046
PharmGKBiPA165450395

Polymorphism and mutation databases

BioMutaiNUPR1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580071 – 82Nuclear protein 1Add BLAST82

Post-translational modificationi

Phosphorylated in vitro by PKA and CK. Phosphorylation promotes DNA-binding activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiO60356
PRIDEiO60356

PTM databases

iPTMnetiO60356
PhosphoSitePlusiO60356

Expressioni

Tissue specificityi

Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes.1 Publication

Inductioni

Up-regulated by stress agents, such as nutrient deprivation (at protein level). Up-regulation by gamma-irradiation is eventually followed by down-regulation.2 Publications

Gene expression databases

BgeeiENSG00000176046
ExpressionAtlasiO60356 baseline and differential
GenevisibleiO60356 HS

Organism-specific databases

HPAiHPA053895

Interactioni

Subunit structurei

Monomer. Directly interacts with MSL1 and binds MORF4L1, two components of histone acetyltransferase complex; the interaction with MORF4L1 may be mediated by MSL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTMAP064547EBI-3908808,EBI-2682091

Protein-protein interaction databases

BioGridi117695, 25 interactors
DIPiDIP-61114N
IntActiO60356, 19 interactors

Structurei

3D structure databases

DisProtiDP00510
ProteinModelPortaliO60356
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi65 – 82Nuclear localization signalSequence analysisAdd BLAST18

Domaini

Has been proposed to exhibit the features of HMG-I(Y) proteins.1 Publication

Sequence similaritiesi

Belongs to the NUPR family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000064242
HOGENOMiHOG000007609
HOVERGENiHBG052704
InParanoidiO60356
KOiK15626
OMAiRHSPGGH
OrthoDBiEOG091G0X6Z
PhylomeDBiO60356
TreeFamiTF324649

Family and domain databases

InterProiView protein in InterPro
IPR018792 NUPR1-like
PfamiView protein in Pfam
PF10195 Phospho_p8, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATFPPATSA PQQPPGPEDE DSSLDESDLY SLAHSYLGGG GRKGRTKREA
60 70 80
AANTNRPSPG GHERKLVTKL QNSERKKRGA RR
Length:82
Mass (Da):8,873
Last modified:August 1, 1998 - v1
Checksum:i596E342D10F87AF3
GO
Isoform 2 (identifier: O60356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-37: L → LGPLIMPMPTSPLTPALVT

Note: No experimental confirmation available.
Show »
Length:100
Mass (Da):10,691
Checksum:i471B40A1607D539F
GO

Sequence cautioni

The sequence AAC05335 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47K → N in BAG35071 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05381637L → LGPLIMPMPTSPLTPALVT in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069073 mRNA Translation: AAC19384.1
AF069074 Genomic DNA Translation: AAC19385.1
AF135266 mRNA Translation: AAD49221.1
AK312135 mRNA Translation: BAG35071.1
BM792961 mRNA No translation available.
BT006896 mRNA Translation: AAP35542.1
CR542144 mRNA Translation: CAG46941.1
AC002425 Genomic DNA Translation: AAC05336.1
AC002425 Genomic DNA Translation: AAC05335.1 Sequence problems.
CH471279 Genomic DNA Translation: EAW52274.1
BC002434 mRNA Translation: AAH02434.1
CCDSiCCDS10634.1 [O60356-1]
CCDS42137.1 [O60356-2]
RefSeqiNP_001035948.1, NM_001042483.1 [O60356-2]
NP_036517.1, NM_012385.2 [O60356-1]
UniGeneiHs.513463

Genome annotation databases

EnsembliENST00000324873; ENSP00000315559; ENSG00000176046 [O60356-1]
ENST00000395641; ENSP00000379003; ENSG00000176046 [O60356-2]
GeneIDi26471
KEGGihsa:26471
UCSCiuc002dqd.2 human [O60356-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUPR1_HUMAN
AccessioniPrimary (citable) accession number: O60356
Secondary accession number(s): B2R5C4, O60357, Q6FGG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health