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Protein

Nuclear protein 1

Gene

NUPR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chromatin-binding protein that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Interacts with MSL1 and inhibits its activity on histone H4 'Lys-16' acetylation (H4K16ac). Binds the RELB promoter and activates its transcription, leading to the transactivation of IER3. The NUPR1/RELB/IER3 survival pathway may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. In breast cancer cells, NUPR1 overexpression leads to the activation of PI3K/AKT signaling pathway, CDKN1A/p21 phosphorylation and relocalization from the nucleus to the cytoplasm, leading to resistance to chemotherapeutic agents, such as doxorubicin.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein 1Curated
Alternative name(s):
Candidate of metastasis 1
Nuclear transcriptional regulator protein 1Imported
Protein p8
Gene namesi
Name:NUPR1Imported
Synonyms:COM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:29990. NUPR1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165450395.

Polymorphism and mutation databases

BioMutaiNUPR1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282Nuclear protein 1PRO_0000058007Add
BLAST

Post-translational modificationi

Phosphorylated in vitro by PKA and CK. Phosphorylation promotes DNA-binding activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO60356.

PTM databases

iPTMnetiO60356.
PhosphoSiteiO60356.

Expressioni

Tissue specificityi

Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes.1 Publication

Inductioni

Up-regulated by stress agents, such as nutrient deprivation (at protein level). Up-regulation by gamma-irradiation is eventually followed by down-regulation.2 Publications

Gene expression databases

BgeeiO60356.
ExpressionAtlasiO60356. baseline and differential.
GenevisibleiO60356. HS.

Interactioni

Subunit structurei

Monomer. Directly interacts with MSL1 and binds MORF4L1, two components of histone acetyltransferase complex; the interaction with MORF4L1 may be mediated by MSL1.1 Publication

Protein-protein interaction databases

BioGridi117695. 25 interactions.
DIPiDIP-61114N.
IntActiO60356. 4 interactions.

Structurei

3D structure databases

DisProtiDP00510.
ProteinModelPortaliO60356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 8218Nuclear localization signalSequence analysisAdd
BLAST

Domaini

Has been proposed to exhibit the features of HMG-I(Y) proteins.1 Publication

Sequence similaritiesi

Belongs to the NUPR family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000064242.
HOGENOMiHOG000007609.
HOVERGENiHBG052704.
InParanoidiO60356.
KOiK15626.
OMAiKFQNSER.
OrthoDBiEOG7VHT18.
PhylomeDBiO60356.
TreeFamiTF324649.

Family and domain databases

InterProiIPR018792. NUPR1-like.
[Graphical view]
PfamiPF10195. Phospho_p8. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATFPPATSA PQQPPGPEDE DSSLDESDLY SLAHSYLGGG GRKGRTKREA
60 70 80
AANTNRPSPG GHERKLVTKL QNSERKKRGA RR
Length:82
Mass (Da):8,873
Last modified:August 1, 1998 - v1
Checksum:i596E342D10F87AF3
GO
Isoform 2 (identifier: O60356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-37: L → LGPLIMPMPTSPLTPALVT

Note: No experimental confirmation available.
Show »
Length:100
Mass (Da):10,691
Checksum:i471B40A1607D539F
GO

Sequence cautioni

The sequence AAC05335.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471K → N in BAG35071 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei37 – 371L → LGPLIMPMPTSPLTPALVT in isoform 2. 1 PublicationVSP_053816

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069073 mRNA. Translation: AAC19384.1.
AF069074 Genomic DNA. Translation: AAC19385.1.
AF135266 mRNA. Translation: AAD49221.1.
AK312135 mRNA. Translation: BAG35071.1.
BM792961 mRNA. No translation available.
BT006896 mRNA. Translation: AAP35542.1.
CR542144 mRNA. Translation: CAG46941.1.
AC002425 Genomic DNA. Translation: AAC05336.1.
AC002425 Genomic DNA. Translation: AAC05335.1. Sequence problems.
CH471279 Genomic DNA. Translation: EAW52274.1.
BC002434 mRNA. Translation: AAH02434.1.
CCDSiCCDS10634.1. [O60356-1]
CCDS42137.1. [O60356-2]
RefSeqiNP_001035948.1. NM_001042483.1. [O60356-2]
NP_036517.1. NM_012385.2. [O60356-1]
UniGeneiHs.513463.

Genome annotation databases

EnsembliENST00000324873; ENSP00000315559; ENSG00000176046. [O60356-1]
ENST00000395641; ENSP00000379003; ENSG00000176046. [O60356-2]
GeneIDi26471.
KEGGihsa:26471.
UCSCiuc002dqd.2. human. [O60356-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069073 mRNA. Translation: AAC19384.1.
AF069074 Genomic DNA. Translation: AAC19385.1.
AF135266 mRNA. Translation: AAD49221.1.
AK312135 mRNA. Translation: BAG35071.1.
BM792961 mRNA. No translation available.
BT006896 mRNA. Translation: AAP35542.1.
CR542144 mRNA. Translation: CAG46941.1.
AC002425 Genomic DNA. Translation: AAC05336.1.
AC002425 Genomic DNA. Translation: AAC05335.1. Sequence problems.
CH471279 Genomic DNA. Translation: EAW52274.1.
BC002434 mRNA. Translation: AAH02434.1.
CCDSiCCDS10634.1. [O60356-1]
CCDS42137.1. [O60356-2]
RefSeqiNP_001035948.1. NM_001042483.1. [O60356-2]
NP_036517.1. NM_012385.2. [O60356-1]
UniGeneiHs.513463.

3D structure databases

DisProtiDP00510.
ProteinModelPortaliO60356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117695. 25 interactions.
DIPiDIP-61114N.
IntActiO60356. 4 interactions.

PTM databases

iPTMnetiO60356.
PhosphoSiteiO60356.

Polymorphism and mutation databases

BioMutaiNUPR1.

Proteomic databases

PRIDEiO60356.

Protocols and materials databases

DNASUi26471.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324873; ENSP00000315559; ENSG00000176046. [O60356-1]
ENST00000395641; ENSP00000379003; ENSG00000176046. [O60356-2]
GeneIDi26471.
KEGGihsa:26471.
UCSCiuc002dqd.2. human. [O60356-1]

Organism-specific databases

CTDi26471.
GeneCardsiNUPR1.
HGNCiHGNC:29990. NUPR1.
MIMi614812. gene.
neXtProtiNX_O60356.
PharmGKBiPA165450395.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00530000064242.
HOGENOMiHOG000007609.
HOVERGENiHBG052704.
InParanoidiO60356.
KOiK15626.
OMAiKFQNSER.
OrthoDBiEOG7VHT18.
PhylomeDBiO60356.
TreeFamiTF324649.

Miscellaneous databases

GeneWikiiNUPR1.
GenomeRNAii26471.
PROiO60356.
SOURCEiSearch...

Gene expression databases

BgeeiO60356.
ExpressionAtlasiO60356. baseline and differential.
GenevisibleiO60356. HS.

Family and domain databases

InterProiIPR018792. NUPR1-like.
[Graphical view]
PfamiPF10195. Phospho_p8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the human p8, a nuclear protein with mitogenic activity."
    Vasseur S., Mallo G.V., Fiedler F., Boedeker H., Canepa E., Moreno S., Iovanna J.L.
    Eur. J. Biochem. 259:670-675(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Pancreas.
  2. "Expression of a novel factor in human breast cancer cells with metastatic potential."
    Ree A.H., Tvermyr M., Engebraaten O., Rooman M., Rosok O., Hovig E., Meza-Zepeda L.A., Bruland O.S., Fodstad O.
    Cancer Res. 59:4675-4680(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Mammary cancer.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Cerebellum.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  10. "Human p8 is a HMG-I/Y-like protein with DNA binding activity enhanced by phosphorylation."
    Encinar J.A., Mallo G.V., Mizyrycki C., Giono L., Gonzalez-Ros J.M., Rico M., Canepa E., Moreno S., Neira J.L., Iovanna J.L.
    J. Biol. Chem. 276:2742-2751(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, MONOMER, PHOSPHORYLATION.
  11. "p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage."
    Gironella M., Malicet C., Cano C., Sandi M.J., Hamidi T., Tauil R.M., Baston M., Valaco P., Moreno S., Lopez F., Neira J.L., Dagorn J.C., Iovanna J.L.
    J. Cell. Physiol. 221:594-602(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MSL1 AND MORF4L1, INDUCTION BY GAMMA-IRRADIATION.
  12. "Cytoplasmic translocation of p21 mediates NUPR1-induced chemoresistance: NUPR1 and p21 in chemoresistance."
    Vincent A.J., Ren S., Harris L.G., Devine D.J., Samant R.S., Fodstad O., Shevde L.A.
    FEBS Lett. 586:3429-3434(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Nuclear protein 1 promotes pancreatic cancer development and protects cells from stress by inhibiting apoptosis."
    Hamidi T., Algul H., Cano C.E., Sandi M.J., Molejon M.I., Riemann M., Calvo E.L., Lomberk G., Dagorn J.C., Weih F., Urrutia R., Schmid R.M., Iovanna J.L.
    J. Clin. Invest. 122:2092-2103(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY NUTRIENT DEPRIVATION.

Entry informationi

Entry nameiNUPR1_HUMAN
AccessioniPrimary (citable) accession number: O60356
Secondary accession number(s): B2R5C4, O60357, Q6FGG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.