ID   PCDGC_HUMAN             Reviewed;         932 AA.
AC   O60330; O15100; Q6UW70; Q9Y5D7;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   27-MAR-2024, entry version 180.
DE   RecName: Full=Protocadherin gamma-A12;
DE            Short=PCDH-gamma-A12;
DE   AltName: Full=Cadherin-21;
DE   AltName: Full=Fibroblast cadherin-3;
DE   Flags: Precursor;
GN   Name=PCDHGA12; Synonyms=CDH21, FIB3, KIAA0588; ORFNames=UNQ371/PRO707;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain;
RX   PubMed=10380929; DOI=10.1016/s0092-8674(00)80789-8;
RA   Wu Q., Maniatis T.;
RT   "A striking organization of a large family of human neural cadherin-like
RT   cell adhesion genes.";
RL   Cell 97:779-790(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=9628581; DOI=10.1093/dnares/5.1.31;
RA   Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N.,
RA   Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. IX. The
RT   complete sequences of 100 new cDNA clones from brain which can code for
RT   large proteins in vitro.";
RL   DNA Res. 5:31-39(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-808.
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 311-444.
RX   PubMed=9199196; DOI=10.1006/bbrc.1997.6707;
RA   Matsuyoshi N., Imamura S.;
RT   "Multiple cadherins are expressed in human fibroblasts.";
RL   Biochem. Biophys. Res. Commun. 235:355-358(1997).
CC   -!- FUNCTION: Potential calcium-dependent cell-adhesion protein. May be
CC       involved in the establishment and maintenance of specific neuronal
CC       connections in the brain.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC       membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O60330-1; Sequence=Displayed;
CC       Name=2; Synonyms=Short;
CC         IsoId=O60330-2; Sequence=VSP_008682, VSP_008683;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAQ89305.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC       Sequence=BAA25514.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF152321; AAD43715.1; -; mRNA.
DR   EMBL; AF152506; AAD43767.1; -; mRNA.
DR   EMBL; AB011160; BAA25514.2; ALT_INIT; mRNA.
DR   EMBL; AY358946; AAQ89305.1; ALT_SEQ; mRNA.
DR   EMBL; AB000897; BAA21135.1; -; mRNA.
DR   CCDS; CCDS4260.1; -. [O60330-1]
DR   CCDS; CCDS75346.1; -. [O60330-2]
DR   PIR; PC4299; PC4299.
DR   RefSeq; NP_003726.1; NM_003735.2. [O60330-1]
DR   RefSeq; NP_115265.1; NM_032094.1. [O60330-2]
DR   AlphaFoldDB; O60330; -.
DR   SMR; O60330; -.
DR   BioGRID; 117494; 9.
DR   IntAct; O60330; 2.
DR   STRING; 9606.ENSP00000252085; -.
DR   GlyCosmos; O60330; 4 sites, 1 glycan.
DR   GlyGen; O60330; 4 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; O60330; -.
DR   PhosphoSitePlus; O60330; -.
DR   BioMuta; PCDHGA12; -.
DR   EPD; O60330; -.
DR   jPOST; O60330; -.
DR   MassIVE; O60330; -.
DR   MaxQB; O60330; -.
DR   PaxDb; 9606-ENSP00000252085; -.
DR   PeptideAtlas; O60330; -.
DR   ProteomicsDB; 49346; -. [O60330-1]
DR   ProteomicsDB; 49347; -. [O60330-2]
DR   Antibodypedia; 57527; 19 antibodies from 6 providers.
DR   DNASU; 26025; -.
DR   Ensembl; ENST00000252085.4; ENSP00000252085.3; ENSG00000253159.3. [O60330-1]
DR   Ensembl; ENST00000613314.1; ENSP00000481438.1; ENSG00000253159.3. [O60330-2]
DR   GeneID; 26025; -.
DR   KEGG; hsa:26025; -.
DR   MANE-Select; ENST00000252085.4; ENSP00000252085.3; NM_003735.3; NP_003726.1.
DR   UCSC; uc003lkt.3; human. [O60330-1]
DR   AGR; HGNC:8699; -.
DR   CTD; 26025; -.
DR   DisGeNET; 26025; -.
DR   GeneCards; PCDHGA12; -.
DR   HGNC; HGNC:8699; PCDHGA12.
DR   HPA; ENSG00000253159; Tissue enhanced (brain).
DR   MalaCards; PCDHGA12; -.
DR   MIM; 603059; gene.
DR   MIM; 604968; gene.
DR   neXtProt; NX_O60330; -.
DR   OpenTargets; ENSG00000253159; -.
DR   PharmGKB; PA33047; -.
DR   VEuPathDB; HostDB:ENSG00000253159; -.
DR   eggNOG; KOG3594; Eukaryota.
DR   GeneTree; ENSGT00940000164062; -.
DR   HOGENOM; CLU_006480_3_0_1; -.
DR   InParanoid; O60330; -.
DR   OMA; APYFRED; -.
DR   OrthoDB; 5402790at2759; -.
DR   PhylomeDB; O60330; -.
DR   TreeFam; TF332299; -.
DR   PathwayCommons; O60330; -.
DR   SignaLink; O60330; -.
DR   SIGNOR; O60330; -.
DR   BioGRID-ORCS; 26025; 10 hits in 1094 CRISPR screens.
DR   GeneWiki; PCDHGA12; -.
DR   GenomeRNAi; 26025; -.
DR   Pharos; O60330; Tdark.
DR   PRO; PR:O60330; -.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; O60330; Protein.
DR   Bgee; ENSG00000253159; Expressed in stromal cell of endometrium and 97 other cell types or tissues.
DR   GO; GO:0005911; C:cell-cell junction; IEA:Ensembl.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; IBA:GO_Central.
DR   GO; GO:0007156; P:homophilic cell adhesion via plasma membrane adhesion molecules; IEA:InterPro.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProt.
DR   CDD; cd11304; Cadherin_repeat; 6.
DR   Gene3D; 2.60.40.60; Cadherins; 6.
DR   InterPro; IPR002126; Cadherin-like_dom.
DR   InterPro; IPR015919; Cadherin-like_sf.
DR   InterPro; IPR032455; Cadherin_C.
DR   InterPro; IPR031904; Cadherin_CBD.
DR   InterPro; IPR020894; Cadherin_CS.
DR   InterPro; IPR013164; Cadherin_N.
DR   PANTHER; PTHR24028; CADHERIN-87A; 1.
DR   PANTHER; PTHR24028:SF227; PROTOCADHERIN GAMMA-A12; 1.
DR   Pfam; PF00028; Cadherin; 5.
DR   Pfam; PF08266; Cadherin_2; 1.
DR   Pfam; PF16492; Cadherin_C_2; 1.
DR   Pfam; PF15974; Cadherin_tail; 1.
DR   PRINTS; PR00205; CADHERIN.
DR   SMART; SM00112; CA; 6.
DR   SUPFAM; SSF49313; Cadherin-like; 6.
DR   PROSITE; PS00232; CADHERIN_1; 5.
DR   PROSITE; PS50268; CADHERIN_2; 6.
DR   Genevisible; O60330; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Calcium; Cell adhesion; Cell membrane; Glycoprotein;
KW   Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..932
FT                   /note="Protocadherin gamma-A12"
FT                   /id="PRO_0000003970"
FT   TOPO_DOM        30..692
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        693..713
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        714..932
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          30..133
FT                   /note="Cadherin 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   DOMAIN          134..242
FT                   /note="Cadherin 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   DOMAIN          243..347
FT                   /note="Cadherin 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   DOMAIN          348..452
FT                   /note="Cadherin 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   DOMAIN          453..562
FT                   /note="Cadherin 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   DOMAIN          570..683
FT                   /note="Cadherin 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00043"
FT   REGION          803..841
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          902..932
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        813..841
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        265
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        419
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        545
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         809..820
FT                   /note="QAPPNTDWRFSQ -> VSLYQIFFLFFF (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10380929"
FT                   /id="VSP_008682"
FT   VAR_SEQ         821..932
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10380929"
FT                   /id="VSP_008683"
FT   CONFLICT        331
FT                   /note="A -> P (in Ref. 4; BAA21135)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   932 AA;  100955 MW;  8BDA90084785D746 CRC64;
     MIPARLHRDY KGLVLLGILL GTLWETGCTQ IRYSVPEELE KGSRVGDISR DLGLEPRELA
     ERGVRIIPRG RTQLFALNPR SGSLVTAGRI DREELCMGAI KCQLNLDILM EDKVKIYGVE
     VEVRDINDNA PYFRESELEI KISENAATEM RFPLPHAWDP DIGKNSLQSY ELSPNTHFSL
     IVQNGADGSK YPELVLKRAL DREEKAAHHL VLTASDGGDP VRTGTARIRV MVLDANDNAP
     AFAQPEYRAS VPENLALGTQ LLVVNATDPD EGVNAEVRYS FRYVDDKAAQ VFKLDCNSGT
     ISTIGELDHE ESGFYQMEVQ AMDNAGYSAR AKVLITVLDV NDNAPEVVLT SLASSVPENS
     PRGTLIALLN VNDQDSEENG QVICFIQGNL PFKLEKSYGN YYSLVTDIVL DREQVPSYNI
     TVTATDRGTP PLSTETHISL NVADTNDNPP VFPQASYSAY IPENNPRGVS LVSVTAHDPD
     CEENAQITYS LAENTIQGAS LSSYVSINSD TGVLYALSSF DYEQFRDLQV KVMARDNGHP
     PLSSNVSLSL FVLDQNDNAP EILYPALPTD GSTGVELAPR SAEPGYLVTK VVAVDRDSGQ
     NAWLSYRLLK ASEPGLFSVG LHTGEVRTAR ALLDRDALKQ SLVVAVQDHG QPPLSATVTL
     TVAVADSIPQ VLADLGSLES PANSETSDLT LYLVVAVAAV SCVFLAFVIL LLALRLRRWH
     KSRLLQASGG GLTGAPASHF VGVDGVQAFL QTYSHEVSLT TDSRKSHLIF PQPNYADMLV
     SQESFEKSEP LLLSGDSVFS KDSHGLIEQA PPNTDWRFSQ AQRPGTSGSQ NGDDTGTWPN
     NQFDTEMLQA MILASASEAA DGSSTLGGGA GTMGLSARYG PQFTLQHVPD YRQNVYIPGS
     NATLTNAAGK RDGKAPAGGN GNKKKSGKKE KK
//