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Protein

Germinal-center associated nuclear protein

Gene

MCM3AP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.By similarity1 Publication
Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.1 Publication

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • nucleotide binding Source: InterPro
  • transferase activity, transferring acyl groups Source: UniProtKB-KW

GO - Biological processi

  • DNA replication Source: ProtInc
  • immune system process Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • protein import into nucleus Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Immunity, mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160294-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal-center associated nuclear protein
Short name:
GANP
Alternative name(s):
80 kDa MCM3-associated protein
MCM3 acetylating protein (EC:2.3.1.-)
Short name:
MCM3AP
MCM3 acetyltransferase
Gene namesi
Name:MCM3AP
Synonyms:GANP, KIAA0572, MAP80
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:6946. MCM3AP.

Subcellular locationi

Isoform GANP :
Isoform MCM3AP :

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB
  • nuclear membrane Source: HPA
  • nuclear pore Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1730 – 1733HGAG → AAAA: Severely decreased MCM3 acetylating activity. 1 Publication4

Organism-specific databases

DisGeNETi8888.
OpenTargetsiENSG00000160294.
PharmGKBiPA30692.

Polymorphism and mutation databases

BioMutaiMCM3AP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000962841 – 1980Germinal-center associated nuclear proteinAdd BLAST1980

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32Asymmetric dimethylarginineBy similarity1
Modified residuei430PhosphoserineCombined sources1
Modified residuei489N6-acetyllysineBy similarity1
Modified residuei490N6-acetyllysineBy similarity1
Modified residuei508PhosphoserineBy similarity1
Modified residuei538PhosphoserineCombined sources1
Modified residuei557PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-508 by CDKs is required for DNA primase activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO60318.
MaxQBiO60318.
PaxDbiO60318.
PeptideAtlasiO60318.
PRIDEiO60318.

PTM databases

iPTMnetiO60318.
PhosphoSitePlusiO60318.

Expressioni

Gene expression databases

BgeeiENSG00000160294.
CleanExiHS_MCM3AP.
ExpressionAtlasiO60318. baseline and differential.
GenevisibleiO60318. HS.

Organism-specific databases

HPAiHPA021527.
HPA032103.

Interactioni

Subunit structurei

Interacts with AICDA/AID (By similarity). Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, DSS1, and either centrin CETN2 or CETN3. Isoform MCM3AP interacts with MCM3. Interacts with NXF1 (By via FG-repeats).By similarity3 Publications

Protein-protein interaction databases

BioGridi114406. 36 interactors.
DIPiDIP-31696N.
IntActiO60318. 34 interactors.
MINTiMINT-1180375.
STRINGi9606.ENSP00000291688.

Structurei

Secondary structure

11980
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1168 – 1232Combined sources65

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DHXX-ray2.10A/D1163-1235[»]
ProteinModelPortaliO60318.
SMRiO60318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 340FG-repeatsAdd BLAST308
Regioni421 – 557DNA primaseAdd BLAST137
Regioni636 – 990SAC3 homologyAdd BLAST355
Regioni1162 – 1256CIDAdd BLAST95
Regioni1658 – 1790AcetyltransferaseAdd BLAST133

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1133 – 1170Sequence analysisAdd BLAST38

Sequence similaritiesi

Belongs to the SAC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1860. Eukaryota.
COG5079. LUCA.
GeneTreeiENSGT00530000063781.
HOGENOMiHOG000113500.
HOVERGENiHBG052431.
InParanoidiO60318.
OMAiFCASEAE.
OrthoDBiEOG091G01V4.
PhylomeDBiO60318.
TreeFamiTF105948.

Family and domain databases

InterProiIPR031910. GANP_CID_dom.
IPR031907. MCM3AP_GANP.
IPR012677. Nucleotide-bd_a/b_plait.
IPR031908. NupH_GANP.
IPR005062. SAC3/GANP/THP3.
[Graphical view]
PfamiPF16766. CID_GANP. 1 hit.
PF16769. MCM3AP_GANP. 1 hit.
PF16768. NupH_GANP. 1 hit.
PF03399. SAC3_GANP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform GANP (identifier: O60318-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPTNPFSGQ QPSAFSASSS NVGTLPSKPP FRFGQPSLFG QNSTLSGKSS
60 70 80 90 100
GFSQVSSFPA SSGVSHSSSV QTLGFTQTSS VGPFSGLEHT STFVATSGPS
110 120 130 140 150
SSSVLGNTGF SFKSPTSVGA FPSTSAFGQE AGEIVNSGFG KTEFSFKPLE
160 170 180 190 200
NAVFKPILGA ESEPEKTQSQ IASGFFTFSH PISSAPGGLA PFSFPQVTSS
210 220 230 240 250
SATTSNFTFS KPVSSNNSLS AFTPALSNQN VEEEKRGPKS IFGSSNNSFS
260 270 280 290 300
SFPVSSAVLG EPFQASKAGV RQGCEEAVSQ VEPLPSLMKG LKRKEDQDRS
310 320 330 340 350
PRRHGHEPAE DSDPLSRGDH PPDKRPVRLN RPRGGTLFGR TIQDVFKSNK
360 370 380 390 400
EVGRLGNKEA KKETGFVESA ESDHMAIPGG NQSVLAPSRI PGVNKEEETE
410 420 430 440 450
SREKKEDSLR GTPARQSNRS ESTDSLGGLS PSEVTAIQCK NIPDYLNDRT
460 470 480 490 500
ILENHFGKIA KVQRIFTRRS KKLAVVHFFD HASAALARKK GKSLHKDMAI
510 520 530 540 550
FWHRKKISPN KKPFSLKEKK PGDGEVSPST EDAPFQHSPL GKAAGRTGAS
560 570 580 590 600
SLLNKSSPVK KPSLLKAHQF EGDSFDSASE GSEGLGPCVL SLSTLIGTVA
610 620 630 640 650
ETSKEKYRLL DQRDRIMRQA RVKRTDLDKA RTFVGTCLDM CPEKERYMRE
660 670 680 690 700
TRSQLSVFEV VPGTDQVDHA AAVKEYSRSS ADQEEPLPHE LRPLPVLSRT
710 720 730 740 750
MDYLVTQIMD QKEGSLRDWY DFVWNRTRGI RKDITQQHLC DPLTVSLIEK
760 770 780 790 800
CTRFHIHCAH FMCEEPMSSF DAKINNENMT KCLQSLKEMY QDLRNKGVFC
810 820 830 840 850
ASEAEFQGYN VLLSLNKGDI LREVQQFHPA VRNSSEVKFA VQAFAALNSN
860 870 880 890 900
NFVRFFKLVQ SASYLNACLL HCYFSQIRKD ALRALNFAYT VSTQRSTIFP
910 920 930 940 950
LDGVVRMLLF RDCEEATDFL TCHGLTVSDG CVELNRSAFL EPEGLSKTRK
960 970 980 990 1000
SVFITRKLTV SVGEIVNGGP LPPVPRHTPV CSFNSQNKYI GESLAAELPV
1010 1020 1030 1040 1050
STQRPGSDTV GGGRGEECGV EPDAPLSSLP QSLPAPAPSP VPLPPVLALT
1060 1070 1080 1090 1100
PSVAPSLFQL SVQPEPPPPE PVPMYSDEDL AQVVDELIQE ALQRDCEEVG
1110 1120 1130 1140 1150
SAGAAYAAAA LGVSNAAMED LLTAATTGIL RHIAAEEVSK ERERREQERQ
1160 1170 1180 1190 1200
RAEEERLKQE RELVLSELSQ GLAVELMERV MMEFVRETCS QELKNAVETD
1210 1220 1230 1240 1250
QRVRVARCCE DVCAHLVDLF LVEEIFQTAK ETLQELQCFC KYLQRWREAV
1260 1270 1280 1290 1300
TARKKLRRQM RAFPAAPCCV DVSDRLRALA PSAECPIAEE NLARGLLDLG
1310 1320 1330 1340 1350
HAGRLGISCT RLRRLRNKTA HQMKVQHFYQ QLLSDVAWAS LDLPSLVAEH
1360 1370 1380 1390 1400
LPGRQEHVFW KLVLVLPDVE EQSPESCGRI LANWLKVKFM GDEGSVDDTS
1410 1420 1430 1440 1450
SDAGGIQTLS LFNSLSSKGD QMISVNVCIK VAHGALSDGA IDAVETQKDL
1460 1470 1480 1490 1500
LGASGLMLLL PPKMKSEDMA EEDVYWLSAL LQLKQLLQAK PFQPALPLVV
1510 1520 1530 1540 1550
LVPSPGGDAV EKEVEDGLML QDLVSAKLIS DYTVTEIPDT INDLQGSTKV
1560 1570 1580 1590 1600
LQAVQWLVSH CPHSLDLCCQ TLIQYVEDGI GHEFSGRFFH DRRERRLGGL
1610 1620 1630 1640 1650
ASQEPGAIIE LFNSVLQFLA SVVSSEQLCD LSWPVTEFAE AGGSRLLPHL
1660 1670 1680 1690 1700
HWNAPEHLAW LKQAVLGFQL PQMDLPPLGA PWLPVCSMVV QYASQIPSSR
1710 1720 1730 1740 1750
QTQPVLQSQV ENLLHRTYCR WKSKSPSPVH GAGPSVMEIP WDDLIALCIN
1760 1770 1780 1790 1800
HKLRDWTPPR LPVTSEALSE DGQICVYFFK NDLKKYDVPL SWEQARLQTQ
1810 1820 1830 1840 1850
KELQLREGRL AIKPFHPSAN NFPIPLLHMH RNWKRSTECA QEGRIPSTED
1860 1870 1880 1890 1900
LMRGASAEEL LAQCLSSSLL LEKEENKRFE DQLQQWLSED SGAFTDLTSL
1910 1920 1930 1940 1950
PLYLPQTLVS LSHTIEPVMK TSVTTSPQSD MMREQLQLSE ATGTCLGERL
1960 1970 1980
KHLERLIRSS REEEVASELH LSALLDMVDI
Length:1,980
Mass (Da):218,405
Last modified:May 30, 2000 - v2
Checksum:i503D192686FC38A8
GO
Isoform MCM3AP (identifier: O60318-2) [UniParc]FASTAAdd to basket
Also known as: 80 kDa MCM3-associated protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-1259: Missing.

Note: Produced via an alternative promoter within an intron of GANP. MCM3AP promoter elements are poorly conserved in mice, suggesting that the regulation of MCM3AP may be human specific.
Show »
Length:721
Mass (Da):80,292
Checksum:i6D50E6EEBB6257C1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019240102S → L.2 PublicationsCorresponds to variant rs9975588dbSNPEnsembl.1
Natural variantiVAR_019241288M → V.1 PublicationCorresponds to variant rs17182545dbSNPEnsembl.1
Natural variantiVAR_019242333R → L.1 PublicationCorresponds to variant rs17182552dbSNPEnsembl.1
Natural variantiVAR_035472409L → V in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs773228537dbSNPEnsembl.1
Natural variantiVAR_019243413P → L.1 PublicationCorresponds to variant rs17182566dbSNPEnsembl.1
Natural variantiVAR_070560915E → K Found in a patient with border-line to mild intellectual disability, progressive polyneuropathy and ptosis; unknown pathological significance. 1 PublicationCorresponds to variant rs483352869dbSNPEnsembl.1
Natural variantiVAR_0192441051P → L.1 PublicationCorresponds to variant rs17182850dbSNPEnsembl.1
Natural variantiVAR_0192451062V → M.1 PublicationCorresponds to variant rs17182857dbSNPEnsembl.1
Natural variantiVAR_0192461314R → W.1 PublicationCorresponds to variant rs17176709dbSNPEnsembl.1
Natural variantiVAR_0192471449D → E.1 PublicationCorresponds to variant rs17183220dbSNPEnsembl.1
Natural variantiVAR_0192481576V → I.2 PublicationsCorresponds to variant rs17183248dbSNPEnsembl.1
Natural variantiVAR_0192491795A → T.1 PublicationCorresponds to variant rs17183290dbSNPEnsembl.1
Natural variantiVAR_0539731831R → C.Corresponds to variant rs2298697dbSNPEnsembl.1
Natural variantiVAR_0192501870L → R.1 PublicationCorresponds to variant rs17176933dbSNPEnsembl.1
Natural variantiVAR_0192511941A → V.1 PublicationCorresponds to variant rs17183403dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0534381 – 1259Missing in isoform MCM3AP. CuratedAdd BLAST1259

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010089 mRNA. Translation: CAB52687.1.
AY590469 Genomic DNA. Translation: AAS89300.1.
AP000471 Genomic DNA. No translation available.
AP001469 Genomic DNA. No translation available.
BC013285 mRNA. Translation: AAH13285.2.
BC004497 mRNA. Translation: AAH04497.2.
BC104958 mRNA. Translation: AAI04959.1.
BC104960 mRNA. Translation: AAI04961.1.
AB011144 mRNA. Translation: BAA25498.1.
AB005543 mRNA. Translation: BAA25170.1.
CCDSiCCDS13734.1. [O60318-1]
PIRiT00339.
RefSeqiNP_003897.2. NM_003906.4. [O60318-1]
XP_005261260.1. XM_005261203.4. [O60318-1]
XP_005261261.1. XM_005261204.4. [O60318-1]
XP_005261262.1. XM_005261205.3. [O60318-1]
UniGeneiHs.389037.
Hs.592438.

Genome annotation databases

EnsembliENST00000291688; ENSP00000291688; ENSG00000160294. [O60318-1]
ENST00000397708; ENSP00000380820; ENSG00000160294. [O60318-1]
GeneIDi8888.
KEGGihsa:8888.
UCSCiuc002zir.3. human. [O60318-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010089 mRNA. Translation: CAB52687.1.
AY590469 Genomic DNA. Translation: AAS89300.1.
AP000471 Genomic DNA. No translation available.
AP001469 Genomic DNA. No translation available.
BC013285 mRNA. Translation: AAH13285.2.
BC004497 mRNA. Translation: AAH04497.2.
BC104958 mRNA. Translation: AAI04959.1.
BC104960 mRNA. Translation: AAI04961.1.
AB011144 mRNA. Translation: BAA25498.1.
AB005543 mRNA. Translation: BAA25170.1.
CCDSiCCDS13734.1. [O60318-1]
PIRiT00339.
RefSeqiNP_003897.2. NM_003906.4. [O60318-1]
XP_005261260.1. XM_005261203.4. [O60318-1]
XP_005261261.1. XM_005261204.4. [O60318-1]
XP_005261262.1. XM_005261205.3. [O60318-1]
UniGeneiHs.389037.
Hs.592438.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DHXX-ray2.10A/D1163-1235[»]
ProteinModelPortaliO60318.
SMRiO60318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114406. 36 interactors.
DIPiDIP-31696N.
IntActiO60318. 34 interactors.
MINTiMINT-1180375.
STRINGi9606.ENSP00000291688.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiO60318.
PhosphoSitePlusiO60318.

Polymorphism and mutation databases

BioMutaiMCM3AP.

Proteomic databases

EPDiO60318.
MaxQBiO60318.
PaxDbiO60318.
PeptideAtlasiO60318.
PRIDEiO60318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291688; ENSP00000291688; ENSG00000160294. [O60318-1]
ENST00000397708; ENSP00000380820; ENSG00000160294. [O60318-1]
GeneIDi8888.
KEGGihsa:8888.
UCSCiuc002zir.3. human. [O60318-1]

Organism-specific databases

CTDi8888.
DisGeNETi8888.
GeneCardsiMCM3AP.
H-InvDBHIX0016189.
HGNCiHGNC:6946. MCM3AP.
HPAiHPA021527.
HPA032103.
MIMi603294. gene.
neXtProtiNX_O60318.
OpenTargetsiENSG00000160294.
PharmGKBiPA30692.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1860. Eukaryota.
COG5079. LUCA.
GeneTreeiENSGT00530000063781.
HOGENOMiHOG000113500.
HOVERGENiHBG052431.
InParanoidiO60318.
OMAiFCASEAE.
OrthoDBiEOG091G01V4.
PhylomeDBiO60318.
TreeFamiTF105948.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160294-MONOMER.

Miscellaneous databases

ChiTaRSiMCM3AP. human.
GeneWikiiMCM3AP.
GenomeRNAii8888.
PROiO60318.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160294.
CleanExiHS_MCM3AP.
ExpressionAtlasiO60318. baseline and differential.
GenevisibleiO60318. HS.

Family and domain databases

InterProiIPR031910. GANP_CID_dom.
IPR031907. MCM3AP_GANP.
IPR012677. Nucleotide-bd_a/b_plait.
IPR031908. NupH_GANP.
IPR005062. SAC3/GANP/THP3.
[Graphical view]
PfamiPF16766. CID_GANP. 1 hit.
PF16769. MCM3AP_GANP. 1 hit.
PF16768. NupH_GANP. 1 hit.
PF03399. SAC3_GANP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGANP_HUMAN
AccessioniPrimary (citable) accession number: O60318
Secondary accession number(s): C9JL56
, Q2M3C1, Q6PJP6, Q9BSY5, Q9UMT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.