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O60307

- MAST3_HUMAN

UniProt

O60307 - MAST3_HUMAN

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Protein

Microtubule-associated serine/threonine-protein kinase 3

Gene

MAST3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei396 – 3961ATPPROSITE-ProRule annotation
Active sitei490 – 4901Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3819ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO60307.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 3 (EC:2.7.11.1)
Gene namesi
Name:MAST3
Synonyms:KIAA0561
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:19036. MAST3.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134877725.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13091309Microtubule-associated serine/threonine-protein kinase 3PRO_0000086314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461Phosphoserine1 Publication
Modified residuei680 – 6801Phosphoserine1 Publication
Modified residuei774 – 7741Phosphoserine1 Publication
Modified residuei782 – 7821Phosphoserine1 Publication
Modified residuei792 – 7921Phosphoserine1 Publication
Modified residuei793 – 7931Phosphoserine1 Publication
Modified residuei1223 – 12231Phosphoserine2 Publications
Modified residuei1273 – 12731PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60307.
PaxDbiO60307.
PRIDEiO60307.

PTM databases

PhosphoSiteiO60307.

Expressioni

Gene expression databases

BgeeiO60307.
CleanExiHS_MAST3.
ExpressionAtlasiO60307. baseline and differential.
GenevestigatoriO60307.

Organism-specific databases

HPAiHPA035061.
HPA035062.

Interactioni

Subunit structurei

Interacts with PTEN.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTENP604843EBI-311420,EBI-696162

Protein-protein interaction databases

BioGridi116670. 4 interactions.
IntActiO60307. 5 interactions.
STRINGi9606.ENSP00000262811.

Structurei

Secondary structure

1
1309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi185 – 19713Combined sources
Turni201 – 2033Combined sources
Helixi209 – 23022Combined sources
Helixi236 – 25520Combined sources
Helixi259 – 27618Combined sources
Beta strandi951 – 9544Combined sources
Beta strandi962 – 97514Combined sources
Beta strandi977 – 98610Combined sources
Helixi991 – 9955Combined sources
Beta strandi1002 – 10065Combined sources
Helixi1016 – 102510Combined sources
Beta strandi1028 – 10358Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9VNMR-A181-281[»]
3KHFX-ray1.20A/B948-1040[»]
ProteinModelPortaliO60307.
SMRiO60307. Positions 181-281, 286-696, 948-1044.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60307.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 640274Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini641 – 71272AGC-kinase C-terminalAdd
BLAST
Domaini950 – 103889PDZPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi104 – 1096Poly-Ser
Compositional biasi764 – 7674Poly-Ser
Compositional biasi877 – 94165Ser-richAdd
BLAST
Compositional biasi1080 – 114970Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiO60307.
KOiK08789.
OMAiHIPIPAR.
OrthoDBiEOG7PK8ZC.
PhylomeDBiO60307.
TreeFamiTF313149.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60307-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDESSLLRRR GLQKELSLPR RGRGCRSGNR KSLVVGTPSP TLSRPLSPLS
60 70 80 90 100
VPTAGSSPLD SPRNFSAASA LNFPFARRAD GRRWSLASLP SSGYGTNTPS
110 120 130 140 150
STLSSSSSSR ERLHQLPFQP TPDELHFLSK HFRSSENVLD EEGGRSPRLR
160 170 180 190 200
PRSRSLSPGR ATGTFDNEIV MMNHVYRERF PKATAQMEGR LQEFLTAYAP
210 220 230 240 250
GARLALADGV LGFIHHQIVE LARDCLAKSG ENLVTSRYFL EMQEKLERLL
260 270 280 290 300
QDAHERSDSE EVSFIVQLVR KLLIIISRPA RLLECLEFDP EEFYHLLEAA
310 320 330 340 350
EGHAREGQGI KTDLPQYIIG QLGLAKDPLE EMVPLSHLEE EQPPAPESPE
360 370 380 390 400
SRALVGQSRR KPCESDFETI KLISNGAYGA VYLVRHRDTR QRFAIKKINK
410 420 430 440 450
QNLILRNQIQ QVFVERDILT FAENPFVVSM FCSFETRRHL CMVMEYVEGG
460 470 480 490 500
DCATLLKNMG PLPVDMARLY FAETVLALEY LHNYGIVHRD LKPDNLLITS
510 520 530 540 550
LGHIKLTDFG LSKIGLMSMA TNLYEGHIEK DAREFIDKQV CGTPEYIAPE
560 570 580 590 600
VIFRQGYGKP VDWWAMGVVL YEFLVGCVPF FGDTPEELFG QVVSDEIMWP
610 620 630 640 650
EGDEALPADA QDLITRLLRQ SPLDRLGTGG THEVKQHPFF LALDWAGLLR
660 670 680 690 700
HKAEFVPQLE AEDDTSYFDT RSERYRHLGS EDDETNDEES STEIPQFSSC
710 720 730 740 750
SHRFSKVYSS SEFLAVQPTP TFAERSFSED REEGWERSEV DYGRRLSADI
760 770 780 790 800
RLRSWTSSGS SCQSSSSQPE RGPSPSLLNT ISLDTMPKFA FSSEDEGVGP
810 820 830 840 850
GPAGPKRPVF ILGEPDPPPA ATPVMPKPSS LSADTAALSH ARLRSNSIGA
860 870 880 890 900
RHSTPRPLDA GRGRRLGGPR DPAPEKSRAS SSGGSGGGSG GRVPKSASVS
910 920 930 940 950
ALSLIITADD GSGGPLMSPL SPRSLSSNPS SRDSSPSRDP SPVCGSLRPP
960 970 980 990 1000
IVIHSSGKKY GFSLRAIRVY MGDSDVYTVH HVVWSVEDGS PAQEAGLRAG
1010 1020 1030 1040 1050
DLITHINGES VLGLVHMDVV ELLLKSGNKI SLRTTALENT SIKVGPARKN
1060 1070 1080 1090 1100
VAKGRMARRS KRSRRRETQD RRKSLFKKIS KQTSVLHTSR SFSSGLHHSL
1110 1120 1130 1140 1150
SSSESLPGSP THSLSPSPTT PCRSPAPDVP ADTTASPPSA SPSSSSPASP
1160 1170 1180 1190 1200
AAAGHTRPSS LHGLAAKLGP PRPKTGRRKS TSSIPPSPLA CPPISAPPPR
1210 1220 1230 1240 1250
SPSPLPGHPP APARSPRLRR GQSADKLGTG ERLDGEAGRR TRGPEAELVV
1260 1270 1280 1290 1300
MRRLHLSERR DSFKKQEAVQ EVSFDEPQEE ATGLPTSVPQ IAVEGEEAVP

VALGPTGRD
Length:1,309
Mass (Da):143,137
Last modified:November 22, 2005 - v2
Checksum:i87E82F2D032ED122
GO

Sequence cautioni

The sequence AAC62830.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti203 – 2031R → Q.
Corresponds to variant rs35945810 [ dbSNP | Ensembl ].
VAR_051646
Natural varianti861 – 8611G → S.
Corresponds to variant rs8108738 [ dbSNP | Ensembl ].
VAR_051647
Natural varianti883 – 8831G → S.1 Publication
VAR_040786

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005793 Genomic DNA. Translation: AAC62830.1. Different initiation.
AC007192 Genomic DNA. Translation: AAD22670.1.
AC093054 Genomic DNA. No translation available.
AB011133 mRNA. Translation: BAA25487.1.
CCDSiCCDS46014.1.
RefSeqiNP_055831.1. NM_015016.1.
UniGeneiHs.466184.

Genome annotation databases

EnsembliENST00000262811; ENSP00000262811; ENSG00000099308.
GeneIDi23031.
KEGGihsa:23031.
UCSCiuc002nhz.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005793 Genomic DNA. Translation: AAC62830.1 . Different initiation.
AC007192 Genomic DNA. Translation: AAD22670.1 .
AC093054 Genomic DNA. No translation available.
AB011133 mRNA. Translation: BAA25487.1 .
CCDSi CCDS46014.1.
RefSeqi NP_055831.1. NM_015016.1.
UniGenei Hs.466184.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1V9V NMR - A 181-281 [» ]
3KHF X-ray 1.20 A/B 948-1040 [» ]
ProteinModelPortali O60307.
SMRi O60307. Positions 181-281, 286-696, 948-1044.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116670. 4 interactions.
IntActi O60307. 5 interactions.
STRINGi 9606.ENSP00000262811.

Chemistry

BindingDBi O60307.
ChEMBLi CHEMBL2417352.

PTM databases

PhosphoSitei O60307.

Proteomic databases

MaxQBi O60307.
PaxDbi O60307.
PRIDEi O60307.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262811 ; ENSP00000262811 ; ENSG00000099308 .
GeneIDi 23031.
KEGGi hsa:23031.
UCSCi uc002nhz.4. human.

Organism-specific databases

CTDi 23031.
GeneCardsi GC19P018208.
H-InvDB HIX0014904.
HGNCi HGNC:19036. MAST3.
HPAi HPA035061.
HPA035062.
MIMi 612258. gene.
neXtProti NX_O60307.
PharmGKBi PA134877725.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063286.
HOGENOMi HOG000046662.
HOVERGENi HBG052414.
InParanoidi O60307.
KOi K08789.
OMAi HIPIPAR.
OrthoDBi EOG7PK8ZC.
PhylomeDBi O60307.
TreeFami TF313149.

Enzyme and pathway databases

SignaLinki O60307.

Miscellaneous databases

ChiTaRSi MAST3. human.
EvolutionaryTracei O60307.
GenomeRNAii 23031.
NextBioi 44010.
PROi O60307.
SOURCEi Search...

Gene expression databases

Bgeei O60307.
CleanExi HS_MAST3.
ExpressionAtlasi O60307. baseline and differential.
Genevestigatori O60307.

Family and domain databases

Gene3Di 1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1309.
    Tissue: Brain.
  3. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
    Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
    J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTEN.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-680 AND SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774; SER-782; SER-792 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. "Solution structure of putative domain of human KIAA0561 protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (MAR-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 181-281.
  9. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-883.

Entry informationi

Entry nameiMAST3_HUMAN
AccessioniPrimary (citable) accession number: O60307
Secondary accession number(s): Q7LDZ8, Q9UPI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 26, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3