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O60307 (MAST3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated serine/threonine-protein kinase 3

EC=2.7.11.1
Gene names
Name:MAST3
Synonyms:KIAA0561
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Subunit structure

Interacts with PTEN. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PDZ (DHR) domain.

Contains 1 protein kinase domain.

Caution

It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.

Sequence caution

The sequence AAC62830.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PTENP604843EBI-311420,EBI-696162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13091309Microtubule-associated serine/threonine-protein kinase 3
PRO_0000086314

Regions

Domain367 – 640274Protein kinase
Domain641 – 71272AGC-kinase C-terminal
Domain950 – 103889PDZ
Nucleotide binding373 – 3819ATP By similarity
Compositional bias104 – 1096Poly-Ser
Compositional bias764 – 7674Poly-Ser
Compositional bias877 – 94165Ser-rich
Compositional bias1080 – 114970Ser-rich

Sites

Active site4901Proton acceptor By similarity
Binding site3961ATP By similarity

Amino acid modifications

Modified residue1461Phosphoserine Ref.6
Modified residue6801Phosphoserine Ref.6
Modified residue7741Phosphoserine Ref.7
Modified residue7821Phosphoserine Ref.7
Modified residue7921Phosphoserine Ref.7
Modified residue7931Phosphoserine Ref.7
Modified residue12231Phosphoserine Ref.4 Ref.6
Modified residue12731Phosphoserine By similarity

Natural variations

Natural variant2031R → Q.
Corresponds to variant rs35945810 [ dbSNP | Ensembl ].
VAR_051646
Natural variant8611G → S.
Corresponds to variant rs8108738 [ dbSNP | Ensembl ].
VAR_051647
Natural variant8831G → S. Ref.9
VAR_040786

Secondary structure

......................... 1309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O60307 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 87E82F2D032ED122

FASTA1,309143,137
        10         20         30         40         50         60 
MDESSLLRRR GLQKELSLPR RGRGCRSGNR KSLVVGTPSP TLSRPLSPLS VPTAGSSPLD 

        70         80         90        100        110        120 
SPRNFSAASA LNFPFARRAD GRRWSLASLP SSGYGTNTPS STLSSSSSSR ERLHQLPFQP 

       130        140        150        160        170        180 
TPDELHFLSK HFRSSENVLD EEGGRSPRLR PRSRSLSPGR ATGTFDNEIV MMNHVYRERF 

       190        200        210        220        230        240 
PKATAQMEGR LQEFLTAYAP GARLALADGV LGFIHHQIVE LARDCLAKSG ENLVTSRYFL 

       250        260        270        280        290        300 
EMQEKLERLL QDAHERSDSE EVSFIVQLVR KLLIIISRPA RLLECLEFDP EEFYHLLEAA 

       310        320        330        340        350        360 
EGHAREGQGI KTDLPQYIIG QLGLAKDPLE EMVPLSHLEE EQPPAPESPE SRALVGQSRR 

       370        380        390        400        410        420 
KPCESDFETI KLISNGAYGA VYLVRHRDTR QRFAIKKINK QNLILRNQIQ QVFVERDILT 

       430        440        450        460        470        480 
FAENPFVVSM FCSFETRRHL CMVMEYVEGG DCATLLKNMG PLPVDMARLY FAETVLALEY 

       490        500        510        520        530        540 
LHNYGIVHRD LKPDNLLITS LGHIKLTDFG LSKIGLMSMA TNLYEGHIEK DAREFIDKQV 

       550        560        570        580        590        600 
CGTPEYIAPE VIFRQGYGKP VDWWAMGVVL YEFLVGCVPF FGDTPEELFG QVVSDEIMWP 

       610        620        630        640        650        660 
EGDEALPADA QDLITRLLRQ SPLDRLGTGG THEVKQHPFF LALDWAGLLR HKAEFVPQLE 

       670        680        690        700        710        720 
AEDDTSYFDT RSERYRHLGS EDDETNDEES STEIPQFSSC SHRFSKVYSS SEFLAVQPTP 

       730        740        750        760        770        780 
TFAERSFSED REEGWERSEV DYGRRLSADI RLRSWTSSGS SCQSSSSQPE RGPSPSLLNT 

       790        800        810        820        830        840 
ISLDTMPKFA FSSEDEGVGP GPAGPKRPVF ILGEPDPPPA ATPVMPKPSS LSADTAALSH 

       850        860        870        880        890        900 
ARLRSNSIGA RHSTPRPLDA GRGRRLGGPR DPAPEKSRAS SSGGSGGGSG GRVPKSASVS 

       910        920        930        940        950        960 
ALSLIITADD GSGGPLMSPL SPRSLSSNPS SRDSSPSRDP SPVCGSLRPP IVIHSSGKKY 

       970        980        990       1000       1010       1020 
GFSLRAIRVY MGDSDVYTVH HVVWSVEDGS PAQEAGLRAG DLITHINGES VLGLVHMDVV 

      1030       1040       1050       1060       1070       1080 
ELLLKSGNKI SLRTTALENT SIKVGPARKN VAKGRMARRS KRSRRRETQD RRKSLFKKIS 

      1090       1100       1110       1120       1130       1140 
KQTSVLHTSR SFSSGLHHSL SSSESLPGSP THSLSPSPTT PCRSPAPDVP ADTTASPPSA 

      1150       1160       1170       1180       1190       1200 
SPSSSSPASP AAAGHTRPSS LHGLAAKLGP PRPKTGRRKS TSSIPPSPLA CPPISAPPPR 

      1210       1220       1230       1240       1250       1260 
SPSPLPGHPP APARSPRLRR GQSADKLGTG ERLDGEAGRR TRGPEAELVV MRRLHLSERR 

      1270       1280       1290       1300 
DSFKKQEAVQ EVSFDEPQEE ATGLPTSVPQ IAVEGEEAVP VALGPTGRD 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1309.
Tissue: Brain.
[3]"Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PTEN.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-680 AND SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774; SER-782; SER-792 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Solution structure of putative domain of human KIAA0561 protein."
RIKEN structural genomics initiative (RSGI)
Submitted (MAR-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 181-281.
[9]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-883.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005793 Genomic DNA. Translation: AAC62830.1. Different initiation.
AC007192 Genomic DNA. Translation: AAD22670.1.
AC093054 Genomic DNA. No translation available.
AB011133 mRNA. Translation: BAA25487.1.
CCDSCCDS46014.1.
RefSeqNP_055831.1. NM_015016.1.
UniGeneHs.466184.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9VNMR-A181-281[»]
3KHFX-ray1.20A/B948-1040[»]
ProteinModelPortalO60307.
SMRO60307. Positions 181-696, 948-1044.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116670. 4 interactions.
IntActO60307. 5 interactions.
STRING9606.ENSP00000262811.

Chemistry

ChEMBLCHEMBL2417352.

PTM databases

PhosphoSiteO60307.

Proteomic databases

MaxQBO60307.
PaxDbO60307.
PRIDEO60307.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262811; ENSP00000262811; ENSG00000099308.
GeneID23031.
KEGGhsa:23031.
UCSCuc002nhz.4. human.

Organism-specific databases

CTD23031.
GeneCardsGC19P018208.
H-InvDBHIX0014904.
HGNCHGNC:19036. MAST3.
HPAHPA035061.
HPA035062.
MIM612258. gene.
neXtProtNX_O60307.
PharmGKBPA134877725.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000046662.
HOVERGENHBG052414.
InParanoidO60307.
KOK08789.
OMAHIPIPAR.
OrthoDBEOG7PK8ZC.
PhylomeDBO60307.
TreeFamTF313149.

Enzyme and pathway databases

SignaLinkO60307.

Gene expression databases

BgeeO60307.
CleanExHS_MAST3.
GenevestigatorO60307.

Family and domain databases

Gene3D1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAST3. human.
EvolutionaryTraceO60307.
GenomeRNAi23031.
NextBio44010.
PROO60307.
SOURCESearch...

Entry information

Entry nameMAST3_HUMAN
AccessionPrimary (citable) accession number: O60307
Secondary accession number(s): Q7LDZ8, Q9UPI0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM