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O60307

- MAST3_HUMAN

UniProt

O60307 - MAST3_HUMAN

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Protein
Microtubule-associated serine/threonine-protein kinase 3
Gene
MAST3, KIAA0561
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei396 – 3961ATP By similarity
Active sitei490 – 4901Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3819ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. protein binding Source: IntAct
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiO60307.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated serine/threonine-protein kinase 3 (EC:2.7.11.1)
    Gene namesi
    Name:MAST3
    Synonyms:KIAA0561
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:19036. MAST3.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134877725.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13091309Microtubule-associated serine/threonine-protein kinase 3
    PRO_0000086314Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei146 – 1461Phosphoserine1 Publication
    Modified residuei680 – 6801Phosphoserine1 Publication
    Modified residuei774 – 7741Phosphoserine1 Publication
    Modified residuei782 – 7821Phosphoserine1 Publication
    Modified residuei792 – 7921Phosphoserine1 Publication
    Modified residuei793 – 7931Phosphoserine1 Publication
    Modified residuei1223 – 12231Phosphoserine2 Publications
    Modified residuei1273 – 12731Phosphoserine By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO60307.
    PaxDbiO60307.
    PRIDEiO60307.

    PTM databases

    PhosphoSiteiO60307.

    Expressioni

    Gene expression databases

    BgeeiO60307.
    CleanExiHS_MAST3.
    GenevestigatoriO60307.

    Organism-specific databases

    HPAiHPA035061.
    HPA035062.

    Interactioni

    Subunit structurei

    Interacts with PTEN.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PTENP604843EBI-311420,EBI-696162

    Protein-protein interaction databases

    BioGridi116670. 4 interactions.
    IntActiO60307. 5 interactions.
    STRINGi9606.ENSP00000262811.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi185 – 19713
    Turni201 – 2033
    Helixi209 – 23022
    Helixi236 – 25520
    Helixi259 – 27618
    Beta strandi951 – 9544
    Beta strandi962 – 97514
    Beta strandi977 – 98610
    Helixi991 – 9955
    Beta strandi1002 – 10065
    Helixi1016 – 102510
    Beta strandi1028 – 10358

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1V9VNMR-A181-281[»]
    3KHFX-ray1.20A/B948-1040[»]
    ProteinModelPortaliO60307.
    SMRiO60307. Positions 181-696, 948-1044.

    Miscellaneous databases

    EvolutionaryTraceiO60307.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini367 – 640274Protein kinase
    Add
    BLAST
    Domaini641 – 71272AGC-kinase C-terminal
    Add
    BLAST
    Domaini950 – 103889PDZ
    Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi104 – 1096Poly-Ser
    Compositional biasi764 – 7674Poly-Ser
    Compositional biasi877 – 94165Ser-rich
    Add
    BLAST
    Compositional biasi1080 – 114970Ser-rich
    Add
    BLAST

    Sequence similaritiesi

    Contains 1 PDZ (DHR) domain.

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000046662.
    HOVERGENiHBG052414.
    InParanoidiO60307.
    KOiK08789.
    OMAiHIPIPAR.
    OrthoDBiEOG7PK8ZC.
    PhylomeDBiO60307.
    TreeFamiTF313149.

    Family and domain databases

    Gene3Di1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60307-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDESSLLRRR GLQKELSLPR RGRGCRSGNR KSLVVGTPSP TLSRPLSPLS     50
    VPTAGSSPLD SPRNFSAASA LNFPFARRAD GRRWSLASLP SSGYGTNTPS 100
    STLSSSSSSR ERLHQLPFQP TPDELHFLSK HFRSSENVLD EEGGRSPRLR 150
    PRSRSLSPGR ATGTFDNEIV MMNHVYRERF PKATAQMEGR LQEFLTAYAP 200
    GARLALADGV LGFIHHQIVE LARDCLAKSG ENLVTSRYFL EMQEKLERLL 250
    QDAHERSDSE EVSFIVQLVR KLLIIISRPA RLLECLEFDP EEFYHLLEAA 300
    EGHAREGQGI KTDLPQYIIG QLGLAKDPLE EMVPLSHLEE EQPPAPESPE 350
    SRALVGQSRR KPCESDFETI KLISNGAYGA VYLVRHRDTR QRFAIKKINK 400
    QNLILRNQIQ QVFVERDILT FAENPFVVSM FCSFETRRHL CMVMEYVEGG 450
    DCATLLKNMG PLPVDMARLY FAETVLALEY LHNYGIVHRD LKPDNLLITS 500
    LGHIKLTDFG LSKIGLMSMA TNLYEGHIEK DAREFIDKQV CGTPEYIAPE 550
    VIFRQGYGKP VDWWAMGVVL YEFLVGCVPF FGDTPEELFG QVVSDEIMWP 600
    EGDEALPADA QDLITRLLRQ SPLDRLGTGG THEVKQHPFF LALDWAGLLR 650
    HKAEFVPQLE AEDDTSYFDT RSERYRHLGS EDDETNDEES STEIPQFSSC 700
    SHRFSKVYSS SEFLAVQPTP TFAERSFSED REEGWERSEV DYGRRLSADI 750
    RLRSWTSSGS SCQSSSSQPE RGPSPSLLNT ISLDTMPKFA FSSEDEGVGP 800
    GPAGPKRPVF ILGEPDPPPA ATPVMPKPSS LSADTAALSH ARLRSNSIGA 850
    RHSTPRPLDA GRGRRLGGPR DPAPEKSRAS SSGGSGGGSG GRVPKSASVS 900
    ALSLIITADD GSGGPLMSPL SPRSLSSNPS SRDSSPSRDP SPVCGSLRPP 950
    IVIHSSGKKY GFSLRAIRVY MGDSDVYTVH HVVWSVEDGS PAQEAGLRAG 1000
    DLITHINGES VLGLVHMDVV ELLLKSGNKI SLRTTALENT SIKVGPARKN 1050
    VAKGRMARRS KRSRRRETQD RRKSLFKKIS KQTSVLHTSR SFSSGLHHSL 1100
    SSSESLPGSP THSLSPSPTT PCRSPAPDVP ADTTASPPSA SPSSSSPASP 1150
    AAAGHTRPSS LHGLAAKLGP PRPKTGRRKS TSSIPPSPLA CPPISAPPPR 1200
    SPSPLPGHPP APARSPRLRR GQSADKLGTG ERLDGEAGRR TRGPEAELVV 1250
    MRRLHLSERR DSFKKQEAVQ EVSFDEPQEE ATGLPTSVPQ IAVEGEEAVP 1300
    VALGPTGRD 1309
    Length:1,309
    Mass (Da):143,137
    Last modified:November 22, 2005 - v2
    Checksum:i87E82F2D032ED122
    GO

    Sequence cautioni

    The sequence AAC62830.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti203 – 2031R → Q.
    Corresponds to variant rs35945810 [ dbSNP | Ensembl ].
    VAR_051646
    Natural varianti861 – 8611G → S.
    Corresponds to variant rs8108738 [ dbSNP | Ensembl ].
    VAR_051647
    Natural varianti883 – 8831G → S.1 Publication
    VAR_040786

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005793 Genomic DNA. Translation: AAC62830.1. Different initiation.
    AC007192 Genomic DNA. Translation: AAD22670.1.
    AC093054 Genomic DNA. No translation available.
    AB011133 mRNA. Translation: BAA25487.1.
    CCDSiCCDS46014.1.
    RefSeqiNP_055831.1. NM_015016.1.
    UniGeneiHs.466184.

    Genome annotation databases

    EnsembliENST00000262811; ENSP00000262811; ENSG00000099308.
    GeneIDi23031.
    KEGGihsa:23031.
    UCSCiuc002nhz.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005793 Genomic DNA. Translation: AAC62830.1 . Different initiation.
    AC007192 Genomic DNA. Translation: AAD22670.1 .
    AC093054 Genomic DNA. No translation available.
    AB011133 mRNA. Translation: BAA25487.1 .
    CCDSi CCDS46014.1.
    RefSeqi NP_055831.1. NM_015016.1.
    UniGenei Hs.466184.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1V9V NMR - A 181-281 [» ]
    3KHF X-ray 1.20 A/B 948-1040 [» ]
    ProteinModelPortali O60307.
    SMRi O60307. Positions 181-696, 948-1044.
    ModBasei Search...

    Protein-protein interaction databases

    BioGridi 116670. 4 interactions.
    IntActi O60307. 5 interactions.
    STRINGi 9606.ENSP00000262811.

    Chemistry

    ChEMBLi CHEMBL2417352.

    PTM databases

    PhosphoSitei O60307.

    Proteomic databases

    MaxQBi O60307.
    PaxDbi O60307.
    PRIDEi O60307.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262811 ; ENSP00000262811 ; ENSG00000099308 .
    GeneIDi 23031.
    KEGGi hsa:23031.
    UCSCi uc002nhz.4. human.

    Organism-specific databases

    CTDi 23031.
    GeneCardsi GC19P018208.
    H-InvDB HIX0014904.
    HGNCi HGNC:19036. MAST3.
    HPAi HPA035061.
    HPA035062.
    MIMi 612258. gene.
    neXtProti NX_O60307.
    PharmGKBi PA134877725.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000046662.
    HOVERGENi HBG052414.
    InParanoidi O60307.
    KOi K08789.
    OMAi HIPIPAR.
    OrthoDBi EOG7PK8ZC.
    PhylomeDBi O60307.
    TreeFami TF313149.

    Enzyme and pathway databases

    SignaLinki O60307.

    Miscellaneous databases

    ChiTaRSi MAST3. human.
    EvolutionaryTracei O60307.
    GenomeRNAii 23031.
    NextBioi 44010.
    PROi O60307.
    SOURCEi Search...

    Gene expression databases

    Bgeei O60307.
    CleanExi HS_MAST3.
    Genevestigatori O60307.

    Family and domain databases

    Gene3Di 1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
      Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1309.
      Tissue: Brain.
    3. "Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases."
      Valiente M., Andres-Pons A., Gomar B., Torres J., Gil A., Tapparel C., Antonarakis S.E., Pulido R.
      J. Biol. Chem. 280:28936-28943(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTEN.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; SER-680 AND SER-1223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-774; SER-782; SER-792 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    8. "Solution structure of putative domain of human KIAA0561 protein."
      RIKEN structural genomics initiative (RSGI)
      Submitted (MAR-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 181-281.
    9. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-883.

    Entry informationi

    Entry nameiMAST3_HUMAN
    AccessioniPrimary (citable) accession number: O60307
    Secondary accession number(s): Q7LDZ8, Q9UPI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: July 9, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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