Reviewed, UniProtKB/Swiss-Prot O60306 (AQR_HUMAN)

Names and origin

Protein names

Recommended name:
    Intron-binding protein aquarius
Alternative name(s):
    Intron-binding protein of 160 kDa
      Short name=IBP160

Gene names

Name:	AQR
Synonyms:	KIAA0560

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Ref.4
Subunit structure	Identified in the spliceosome C complex, at least composed of AQR, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRNP200, SNRNP40, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, XAB2 and ZCCHC8.
Subcellular location	Nucleus. Note: Localizes to speckle-like regions of the nucleoplasm. Ref.4
Sequence similarities	Belongs to the CWF11 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Ligand	RNA-binding
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1485	1485	Intron-binding protein aquarius		PRO_0000252389
Amino acid modifications
	Modified residue	576	1	N6-acetyllysine Ref.6
	Modified residue	1051	1	N6-acetyllysine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O60306-1 [UniParc]. Last modified October 17, 2006. Version 4. Checksum: D0ADDA621A9418FD	FASTA	1,485	171,295
	10 20 30 40 50 60 MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA 70 80 90 100 110 120 IRKIMLLEFS QYLENYLWMN YSPEVSSKAY LMSICCMVNE KFRENVPAWE IFKKKPDHFP 130 140 150 160 170 180 FFFKHILKAA LAETDGEFSL HEQTVLLLFL DHCFNSLEVD LIRSQVQQLI SLPMWMGLQL 190 200 210 220 230 240 ARLELELKKT PKLRKFWNLI KKNDEKMDPE AREQAYQERR FLSQLIQKFI SVLKSVPLSE 250 260 270 280 290 300 PVTMDKVHYC ERFIELMIDL EALLPTRRWF NTILDDSHLL VHCYLSNLVR REEDGHLFSQ 310 320 330 340 350 360 LLDMLKFYTG FEINDQTGNA LTENEMTTIH YDRITSLQRA AFAHFPELYD FALSNVAEVD 370 380 390 400 410 420 TRESLVKFFG PLSSNTLHQV ASYLCLLPTL PKNEDTTFDK EFLLELLVSR HERRISQIQQ 430 440 450 460 470 480 LNQMPLYPTE KIIWDENIVP TEYYSGEGCL ALPKLNLQFL TLHDYLLRNF NLFRLESTYE 490 500 510 520 530 540 IRQDIEDSVS RMKPWQSEYG GVVFGGWARM AQPIVAFTVV EVAKPNIGEN WPTRVRADVT 550 560 570 580 590 600 INLNVRDHIK DEWEGLRKHD VCFLITVRPT KPYGTKFDRR RPFIEQVGLV YVRGCEIQGM 610 620 630 640 650 660 LDDKGRVIED GPEPRPNLRG ESRTFRVFLD PNQYQQDMTN TIQNGAEDVY ETFNIIMRRK 670 680 690 700 710 720 PKENNFKAVL ETIRNLMNTD CVVPDWLHDI ILGYGDPSSA HYSKMPNQIA TLDFNDTFLS 730 740 750 760 770 780 IEHLKASFPG HNVKVTVEDP ALQIPPFRIT FPVRSGKGKK RKDADVEDED TEEAKTLIVE 790 800 810 820 830 840 PHVIPNRGPY PYNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI 850 860 870 880 890 900 YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETEK DFSRYGRVNY 910 920 930 940 950 960 VLARRIELLE EVKRLQKSLG VPGDASYTCE TAGYFFLYQV MSRWEEYISK VKNKGSTLPD 970 980 990 1000 1010 1020 VTEVSTFFPF HEYFANAPQP IFKGRSYEED MEIAEGCFRH IKKIFTQLEE FRASELLRSG 1030 1040 1050 1060 1070 1080 LDRSKYLLVK EAKIIAMTCT HAALKRHDLV KLGFKYDNIL MEEAAQILEI ETFIPLLLQN 1090 1100 1110 1120 1130 1140 PQDGFSRLKR WIMIGDHHQL PPVIKNMAFQ KYSNMEQSLF TRFVRVGVPT VDLDAQGRAR 1150 1160 1170 1180 1190 1200 ASLCNLYNWR YKNLGNLPHV QLLPEFSTAN AGLLYDFQLI NVEDFQGVGE SEPNPYFYQN 1210 1220 1230 1240 1250 1260 LGEAEYVVAL FMYMCLLGYP ADKISILTTY NGQKHLIRDI INRRCGNNPL IGRPNKVTTV 1270 1280 1290 1300 1310 1320 DRFQGQQNDY ILLSLVRTRA VGHLRDVRRL VVAMSRARLG LYIFARVSLF QNCFELTPAF 1330 1340 1350 1360 1370 1380 SQLTARPLHL HIIPTEPFPT TRKNGERPSH EVQIIKNMPQ MANFVYNMYM HLIQTTHHYH 1390 1400 1410 1420 1430 1440 QTLLQLPPAM VEEGEEVQNQ ETELETEEEA MTVQADIIPS PTDTSCRQET PAFQTDTTPS 1450 1460 1470 1480 ETGATSTPEA IPALSETTPT VVGAVSAPAE ANTPQDATSA PEETK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix and Mammary gland.
[3]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
[4]	"A spliceosomal intron binding protein, IBP160, links Position-dependent assembly of intron-encoded box C/D snoRNP to pre-mRNA splicing." Hirose T., Ideue T., Nagai M., Hagiwara M., Shu M.-D., Steitz J.A. Mol. Cell 23:673-684(2006) [PubMed: 16949364] [Abstract] Cited for: FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-576 AND LYS-1051, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB011132 mRNA. Translation: BAA25486.3. Different initiation. BC036913 mRNA. Translation: AAH36913.1. BC070379 mRNA. Translation: AAH70379.1. BC108262 mRNA. Translation: AAI08263.1. BC127111 mRNA. Translation: AAI27112.1. BC127112 mRNA. Translation: AAI27113.1.
IPI	IPI00297572.
PIR	T00333.
RefSeq	NP_055506.1.
UniGene	Hs.510958
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O60306.
Proteomic databases
PRIDE	O60306.
Genome annotation databases
Ensembl	ENST00000156471; ENSP00000156471; ENSG00000021776; Homo sapiens. [Genome view]
GeneID	9716.
KEGG	hsa:9716.
UCSC	uc001ziv.1. human.
Organism-specific databases
CTD	9716.
GeneCards	GC15M032935.
HGNC	HGNC:29513. AQR.
MIM	610548. gene.
PharmGKB	PA134869224.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG319395.
HOVERGEN	O60306.
InParanoid	O60306.
OMA	GDVSYTC.
OrthoDB	EOG9CVJSP.
Gene expression databases
ArrayExpress	O60306.
Bgee	O60306.
CleanEx	HS_AQR.
Genevestigator	O60306.
GermOnline	ENSG00000021776. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	36525.
SOURCE	Search...

Entry information

Entry name

AQR_HUMAN

Accession

Primary (citable) accession number: O60306
Secondary accession number(s): A0JP17 , Q2YDX9, Q6IRU8, Q6PIC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	December 15, 2009
This is version 56 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.