O60296 (TRAK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking kinesin-binding protein 2

Alternative name(s):
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein

Gene names

Name:	TRAK2
Synonyms:	ALS2CR3, KIAA0549

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR By similarity.
Subunit structure	Interacts with GABA-A receptor and O-GlcNAc transferase. Interacts with HGS By similarity. Interacts with RHOT1/Miro-1 and RHOT2/Miro-2. Ref.5
Subcellular location	Cytoplasm By similarity. Early endosome By similarity. Mitochondrion By similarity. Note: Colocalizes with MGARP at the mitochondria. Translocates from the cytoplasm to the mitochondria in a MGARP-dependent manner By similarity.
Tissue specificity	Widely expressed, with highest expression in heart.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Contains 1 HAP1 N-terminal domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Endosome Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from electronic annotation. Source: Compara protein targeting Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	early endosome Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from sequence or structural similarity PubMed 12034717. Source: UniProtKB
Molecular_function	GABA receptor binding Non-traceable author statement PubMed 15644324. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 914	914	Trafficking kinesin-binding protein 2		PRO_0000064538
Regions
	Domain	48 – 353	306	HAP1 N-terminal
	Region	359 – 509	151	Interaction with HGS By similarity
	Coiled coil	134 – 354	221	Potential
Natural variations
	Natural variant	142	1	V → I. Ref.1 Ref.2 Corresponds to variant rs13022344 [ dbSNP | Ensembl ].		VAR_014201
	Natural variant	528	1	T → I. Ref.1 Corresponds to variant rs2244438 [ dbSNP | Ensembl ].		VAR_014434
	Natural variant	863	1	I → N. Ref.4 Corresponds to variant rs34594680 [ dbSNP | Ensembl ].		VAR_051458
Experimental info
	Sequence conflict	301 – 311	11	HVIEKEELKLH → VGLFIHSTDIC Ref.2
	Sequence conflict	312 – 914	603	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O60296 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: C26525C223A70D6D	FASTA	914	101,419
	10 20 30 40 50 60 MSQSQNAIFT SPTGEENLMN SNHRDSESIT DVCSNEDLPE VELVSLLEEQ LPQYRLKVDT 70 80 90 100 110 120 LFLYENQDWT QSPHQRQHAS DALSPVLAEE TFRYMILGTD RVEQMTKTYN DIDMVTHLLA 130 140 150 160 170 180 ERDRDLELAA RIGQALLKRN HVLSEQNESL EEQLGQAFDQ VNQLQHELCK KDELLRIVSI 190 200 210 220 230 240 ASEESETDSS CSTPLRFNES FSLSQGLLQL EMLQEKLKEL EEENMALRSK ACHIKTETVT 250 260 270 280 290 300 YEEKEQQLVS DCVKELRETN AQMSRMTEEL SGKSDELIRY QEELSSLLSQ IVDLQHKLKE 310 320 330 340 350 360 HVIEKEELKL HLQASKDAQR QLTMELHELQ DRNMECLGML HESQEEIKEL RSRSGPTAHL 370 380 390 400 410 420 YFSQSYGAFT GESLAAEIEG TMRKKLSLDE ESSLFKQKAQ QKRVFDTVRI ANDTRGRSIS 430 440 450 460 470 480 FPALLPIPGS NRSSVIMTAK PFESGLQQTE DKSLLNQGSS SEEVAGSSQK MGQPGPSGDS 490 500 510 520 530 540 DLATALHRLS LRRQNYLSEK QFFAEEWQRK IQVLADQKEG VSGCVTPTES LASLCTTQSE 550 560 570 580 590 600 ITDLSSASCL RGFMPEKLQI VKPLEGSQTL YHWQQLAQPN LGTILDPRPG VITKGFTQLP 610 620 630 640 650 660 GDAIYHISDL EEDEEEGITF QVQQPLEVEE KLSTSKPVTG IFLPPITSAG GPVTVATANP 670 680 690 700 710 720 GKCLSCTNST FTFTTCRILH PSDITQVTPS SGFPSLSCGS SGSSSSNTAV NSPALSYRLS 730 740 750 760 770 780 IGESITNRRD STTTFSSTMS LAKLLQERGI SAKVYHSPIS ENPLQPLPKS LAIPSTPPNS 790 800 810 820 830 840 PSHSPCPSPL PFEPRVHLSE NFLASRPAET FLQEMYGLRP SRNPPDVGQL KMNLVDRLKR 850 860 870 880 890 900 LGIARVVKNP GAQENGRCQE AEIGPQKPDS AVYLNSGSSL LGGLRRNQSL PVIMGSFAAP 910 VCTSSPKMGV LKED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2." Hadano S., Yanagisawa Y., Skaug J., Fichter K., Nasir J., Martindale D., Koop B.F., Scherer S.W., Nicholson D.W., Rouleau G.A., Ikeda J.-E., Hayden M.R. Genomics 71:200-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ILE-142 AND ILE-528.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-142. Tissue: Cerebellum.
[3]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 446-914. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 798-914, VARIANT ASN-863. Tissue: Skin.
[5]	"The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking." Fransson S., Ruusala A., Aspenstroem P. Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RHOT1 AND RHOT2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038964 Genomic DNA. Translation: BAB32550.1. AB038951 mRNA. Translation: BAB32501.1. AK054763 mRNA. Translation: BAB70803.1. AB011121 mRNA. Translation: BAA25475.1. BC017999 mRNA. Translation: AAH17999.1.
IPI	IPI00297257.
RefSeq	NP_055864.2. NM_015049.2.
UniGene	Hs.152774.
3D structure databases
ProteinModelPortal	O60296.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60296. 1 interaction.
STRING	9606.ENSP00000328875.
PTM databases
PhosphoSite	O60296.
Proteomic databases
PaxDb	O60296.
PRIDE	O60296.
Protocols and materials databases
DNASU	66008.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000332624; ENSP00000328875; ENSG00000115993.
GeneID	66008.
KEGG	hsa:66008.
UCSC	uc002uyb.4. human.
Organism-specific databases
CTD	66008.
GeneCards	GC02M202241.
H-InvDB	HIX0002738.
HGNC	HGNC:13206. TRAK2.
HPA	HPA015827.
MIM	607334. gene.
neXtProt	NX_O60296.
PharmGKB	PA24744.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG246318.
HOGENOM	HOG000143414.
HOVERGEN	HBG069248.
InParanoid	O60296.
KO	K15374.
OMA	EETFRYM.
OrthoDB	EOG48KR9V.
PhylomeDB	O60296.
Gene expression databases
ArrayExpress	O60296.
Bgee	O60296.
CleanEx	HS_TRAK2.
Genevestigator	O60296.
GermOnline	ENSG00000115993. Homo sapiens.
Family and domain databases
InterPro	IPR006933. HAP1_N. IPR022154. Traffickng_kinesin-bd_prot_dom. [Graphical view]
Pfam	PF04849. HAP1_N. 1 hit. PF12448. Milton. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRAK2. human.
GenomeRNAi	66008.
NextBio	67530.
SOURCE	Search...

Entry information

Entry name

TRAK2_HUMAN

Accession

Primary (citable) accession number: O60296
Secondary accession number(s): Q8WVH7 , Q96NS2, Q9C0K5, Q9C0K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 25, 2002
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.