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O60296 (TRAK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking kinesin-binding protein 2
Alternative name(s):
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein
Gene names
Name:TRAK2
Synonyms:ALS2CR3, KIAA0549
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length914 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR By similarity.

Subunit structure

Interacts with GABA-A receptor and O-GlcNAc transferase. Interacts with HGS By similarity. Interacts with RHOT1/Miro-1 and RHOT2/Miro-2. Ref.6

Subcellular location

Cytoplasm By similarity. Early endosome By similarity. Mitochondrion By similarity. Note: Colocalizes with MGARP at the mitochondria. Translocates from the cytoplasm to the mitochondria in a MGARP-dependent manner By similarity.

Tissue specificity

Widely expressed, with highest expression in heart.

Post-translational modification

O-glycosylated By similarity.

Sequence similarities

Contains 1 HAP1 N-terminal domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60296-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60296-2)

The sequence of this isoform differs from the canonical sequence as follows:
     301-311: HVIEKEELKLH → VGLFIHSTDIC
     312-914: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 914914Trafficking kinesin-binding protein 2
PRO_0000064538

Regions

Domain48 – 353306HAP1 N-terminal
Region359 – 509151Interaction with HGS By similarity
Coiled coil134 – 354221 Potential

Natural variations

Alternative sequence301 – 31111HVIEKEELKLH → VGLFIHSTDIC in isoform 2.
VSP_053418
Alternative sequence312 – 914603Missing in isoform 2.
VSP_053419
Natural variant1421V → I. Ref.1 Ref.2
Corresponds to variant rs13022344 [ dbSNP | Ensembl ].
VAR_014201
Natural variant5281T → I. Ref.1
Corresponds to variant rs2244438 [ dbSNP | Ensembl ].
VAR_014434
Natural variant8631I → N. Ref.5
Corresponds to variant rs34594680 [ dbSNP | Ensembl ].
VAR_051458

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 25, 2002. Version 2.
Checksum: C26525C223A70D6D

FASTA914101,419
        10         20         30         40         50         60 
MSQSQNAIFT SPTGEENLMN SNHRDSESIT DVCSNEDLPE VELVSLLEEQ LPQYRLKVDT 

        70         80         90        100        110        120 
LFLYENQDWT QSPHQRQHAS DALSPVLAEE TFRYMILGTD RVEQMTKTYN DIDMVTHLLA 

       130        140        150        160        170        180 
ERDRDLELAA RIGQALLKRN HVLSEQNESL EEQLGQAFDQ VNQLQHELCK KDELLRIVSI 

       190        200        210        220        230        240 
ASEESETDSS CSTPLRFNES FSLSQGLLQL EMLQEKLKEL EEENMALRSK ACHIKTETVT 

       250        260        270        280        290        300 
YEEKEQQLVS DCVKELRETN AQMSRMTEEL SGKSDELIRY QEELSSLLSQ IVDLQHKLKE 

       310        320        330        340        350        360 
HVIEKEELKL HLQASKDAQR QLTMELHELQ DRNMECLGML HESQEEIKEL RSRSGPTAHL 

       370        380        390        400        410        420 
YFSQSYGAFT GESLAAEIEG TMRKKLSLDE ESSLFKQKAQ QKRVFDTVRI ANDTRGRSIS 

       430        440        450        460        470        480 
FPALLPIPGS NRSSVIMTAK PFESGLQQTE DKSLLNQGSS SEEVAGSSQK MGQPGPSGDS 

       490        500        510        520        530        540 
DLATALHRLS LRRQNYLSEK QFFAEEWQRK IQVLADQKEG VSGCVTPTES LASLCTTQSE 

       550        560        570        580        590        600 
ITDLSSASCL RGFMPEKLQI VKPLEGSQTL YHWQQLAQPN LGTILDPRPG VITKGFTQLP 

       610        620        630        640        650        660 
GDAIYHISDL EEDEEEGITF QVQQPLEVEE KLSTSKPVTG IFLPPITSAG GPVTVATANP 

       670        680        690        700        710        720 
GKCLSCTNST FTFTTCRILH PSDITQVTPS SGFPSLSCGS SGSSSSNTAV NSPALSYRLS 

       730        740        750        760        770        780 
IGESITNRRD STTTFSSTMS LAKLLQERGI SAKVYHSPIS ENPLQPLPKS LAIPSTPPNS 

       790        800        810        820        830        840 
PSHSPCPSPL PFEPRVHLSE NFLASRPAET FLQEMYGLRP SRNPPDVGQL KMNLVDRLKR 

       850        860        870        880        890        900 
LGIARVVKNP GAQENGRCQE AEIGPQKPDS AVYLNSGSSL LGGLRRNQSL PVIMGSFAAP 

       910 
VCTSSPKMGV LKED 

« Hide

Isoform 2 [UniParc].

Checksum: BD6C40048A0FFEEB
Show »

FASTA31135,815

References

« Hide 'large scale' references
[1]"Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2."
Hadano S., Yanagisawa Y., Skaug J., Fichter K., Nasir J., Martindale D., Koop B.F., Scherer S.W., Nicholson D.W., Rouleau G.A., Ikeda J.-E., Hayden M.R.
Genomics 71:200-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ILE-142 AND ILE-528.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ILE-142.
Tissue: Cerebellum.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 446-914 (ISOFORM 1).
Tissue: Brain.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 798-914 (ISOFORM 1), VARIANT ASN-863.
Tissue: Skin.
[6]"The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking."
Fransson S., Ruusala A., Aspenstroem P.
Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RHOT1 AND RHOT2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038964 Genomic DNA. Translation: BAB32550.1.
AB038951 mRNA. Translation: BAB32501.1.
AK054763 mRNA. Translation: BAB70803.1.
AC007256 Genomic DNA. No translation available.
AC007282 Genomic DNA. No translation available.
AC080069 Genomic DNA. No translation available.
AB011121 mRNA. Translation: BAA25475.1.
BC017999 mRNA. Translation: AAH17999.1.
RefSeqNP_055864.2. NM_015049.2.
UniGeneHs.152774.

3D structure databases

ProteinModelPortalO60296.
SMRO60296. Positions 259-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122452. 4 interactions.
IntActO60296. 1 interaction.
STRING9606.ENSP00000328875.

PTM databases

PhosphoSiteO60296.

Proteomic databases

PaxDbO60296.
PRIDEO60296.

Protocols and materials databases

DNASU66008.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000332624; ENSP00000328875; ENSG00000115993. [O60296-1]
ENST00000430254; ENSP00000409333; ENSG00000115993. [O60296-2]
GeneID66008.
KEGGhsa:66008.
UCSCuc002uyb.4. human. [O60296-1]

Organism-specific databases

CTD66008.
GeneCardsGC02M202241.
H-InvDBHIX0002738.
HGNCHGNC:13206. TRAK2.
HPAHPA015827.
MIM607334. gene.
neXtProtNX_O60296.
PharmGKBPA24744.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG246318.
HOGENOMHOG000143414.
HOVERGENHBG069248.
InParanoidO60296.
KOK15374.
OMAEETFRYM.
OrthoDBEOG7CRTSM.
PhylomeDBO60296.
TreeFamTF323495.

Gene expression databases

ArrayExpressO60296.
BgeeO60296.
CleanExHS_TRAK2.
GenevestigatorO60296.

Family and domain databases

InterProIPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
[Graphical view]
PfamPF04849. HAP1_N. 1 hit.
PF12448. Milton. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTRAK2. human.
GeneWikiTRAK2.
GenomeRNAi66008.
NextBio35500578.
PROO60296.
SOURCESearch...

Entry information

Entry nameTRAK2_HUMAN
AccessionPrimary (citable) accession number: O60296
Secondary accession number(s): E7EV21 expand/collapse secondary AC list , Q8WVH7, Q96NS2, Q9C0K5, Q9C0K6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 25, 2002
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM