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Protein

Trafficking kinesin-binding protein 2

Gene

TRAK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR.By similarity

GO - Molecular functioni

  1. GABA receptor binding Source: HGNC
  2. receptor binding Source: UniProtKB

GO - Biological processi

  1. protein O-linked glycosylation Source: Ensembl
  2. protein targeting Source: Ensembl
  3. regulation of transcription from RNA polymerase II promoter Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking kinesin-binding protein 2
Alternative name(s):
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein
Gene namesi
Name:TRAK2
Synonyms:ALS2CR3, KIAA0549
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:13206. TRAK2.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Early endosome By similarity
  3. Mitochondrion By similarity

  4. Note: Colocalizes with MGARP at the mitochondria. Translocates from the cytoplasm to the mitochondria in a MGARP-dependent manner (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. early endosome Source: UniProtKB-SubCell
  3. mitochondrion Source: UniProtKB
  4. nucleus Source: Ensembl
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24744.

Polymorphism and mutation databases

BioMutaiTRAK2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Trafficking kinesin-binding protein 2PRO_0000064538Add
BLAST

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO60296.
PaxDbiO60296.
PRIDEiO60296.

PTM databases

PhosphoSiteiO60296.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in heart.

Gene expression databases

BgeeiO60296.
CleanExiHS_TRAK2.
ExpressionAtlasiO60296. baseline and differential.
GenevestigatoriO60296.

Organism-specific databases

HPAiHPA015827.

Interactioni

Subunit structurei

Interacts with GABA-A receptor and O-GlcNAc transferase. Interacts with HGS (By similarity). Interacts with RHOT1/Miro-1 and RHOT2/Miro-2.By similarity1 Publication

Protein-protein interaction databases

BioGridi122452. 29 interactions.
IntActiO60296. 1 interaction.
STRINGi9606.ENSP00000328875.

Structurei

3D structure databases

ProteinModelPortaliO60296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 353306HAP1 N-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni359 – 509151Interaction with HGSBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili134 – 354221Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 HAP1 N-terminal domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG246318.
GeneTreeiENSGT00390000003132.
HOGENOMiHOG000143414.
HOVERGENiHBG069248.
InParanoidiO60296.
KOiK15374.
OMAiVGQLKMN.
OrthoDBiEOG7CRTSM.
PhylomeDBiO60296.
TreeFamiTF323495.

Family and domain databases

InterProiIPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
[Graphical view]
PfamiPF04849. HAP1_N. 1 hit.
PF12448. Milton. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60296-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSQNAIFT SPTGEENLMN SNHRDSESIT DVCSNEDLPE VELVSLLEEQ
60 70 80 90 100
LPQYRLKVDT LFLYENQDWT QSPHQRQHAS DALSPVLAEE TFRYMILGTD
110 120 130 140 150
RVEQMTKTYN DIDMVTHLLA ERDRDLELAA RIGQALLKRN HVLSEQNESL
160 170 180 190 200
EEQLGQAFDQ VNQLQHELCK KDELLRIVSI ASEESETDSS CSTPLRFNES
210 220 230 240 250
FSLSQGLLQL EMLQEKLKEL EEENMALRSK ACHIKTETVT YEEKEQQLVS
260 270 280 290 300
DCVKELRETN AQMSRMTEEL SGKSDELIRY QEELSSLLSQ IVDLQHKLKE
310 320 330 340 350
HVIEKEELKL HLQASKDAQR QLTMELHELQ DRNMECLGML HESQEEIKEL
360 370 380 390 400
RSRSGPTAHL YFSQSYGAFT GESLAAEIEG TMRKKLSLDE ESSLFKQKAQ
410 420 430 440 450
QKRVFDTVRI ANDTRGRSIS FPALLPIPGS NRSSVIMTAK PFESGLQQTE
460 470 480 490 500
DKSLLNQGSS SEEVAGSSQK MGQPGPSGDS DLATALHRLS LRRQNYLSEK
510 520 530 540 550
QFFAEEWQRK IQVLADQKEG VSGCVTPTES LASLCTTQSE ITDLSSASCL
560 570 580 590 600
RGFMPEKLQI VKPLEGSQTL YHWQQLAQPN LGTILDPRPG VITKGFTQLP
610 620 630 640 650
GDAIYHISDL EEDEEEGITF QVQQPLEVEE KLSTSKPVTG IFLPPITSAG
660 670 680 690 700
GPVTVATANP GKCLSCTNST FTFTTCRILH PSDITQVTPS SGFPSLSCGS
710 720 730 740 750
SGSSSSNTAV NSPALSYRLS IGESITNRRD STTTFSSTMS LAKLLQERGI
760 770 780 790 800
SAKVYHSPIS ENPLQPLPKS LAIPSTPPNS PSHSPCPSPL PFEPRVHLSE
810 820 830 840 850
NFLASRPAET FLQEMYGLRP SRNPPDVGQL KMNLVDRLKR LGIARVVKNP
860 870 880 890 900
GAQENGRCQE AEIGPQKPDS AVYLNSGSSL LGGLRRNQSL PVIMGSFAAP
910
VCTSSPKMGV LKED
Length:914
Mass (Da):101,419
Last modified:October 25, 2002 - v2
Checksum:iC26525C223A70D6D
GO
Isoform 2 (identifier: O60296-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     301-311: HVIEKEELKLH → VGLFIHSTDIC
     312-914: Missing.

Show »
Length:311
Mass (Da):35,815
Checksum:iBD6C40048A0FFEEB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti142 – 1421V → I.2 Publications
Corresponds to variant rs13022344 [ dbSNP | Ensembl ].
VAR_014201
Natural varianti528 – 5281T → I.1 Publication
Corresponds to variant rs2244438 [ dbSNP | Ensembl ].
VAR_014434
Natural varianti863 – 8631I → N.1 Publication
Corresponds to variant rs34594680 [ dbSNP | Ensembl ].
VAR_051458

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei301 – 31111HVIEKEELKLH → VGLFIHSTDIC in isoform 2. 1 PublicationVSP_053418Add
BLAST
Alternative sequencei312 – 914603Missing in isoform 2. 1 PublicationVSP_053419Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038964 Genomic DNA. Translation: BAB32550.1.
AB038951 mRNA. Translation: BAB32501.1.
AK054763 mRNA. Translation: BAB70803.1.
AC007256 Genomic DNA. No translation available.
AC007282 Genomic DNA. No translation available.
AC080069 Genomic DNA. No translation available.
AB011121 mRNA. Translation: BAA25475.1.
BC017999 mRNA. Translation: AAH17999.1.
CCDSiCCDS2347.1. [O60296-1]
RefSeqiNP_055864.2. NM_015049.2. [O60296-1]
UniGeneiHs.152774.

Genome annotation databases

EnsembliENST00000332624; ENSP00000328875; ENSG00000115993. [O60296-1]
ENST00000430254; ENSP00000409333; ENSG00000115993. [O60296-2]
GeneIDi66008.
KEGGihsa:66008.
UCSCiuc002uyb.4. human. [O60296-1]

Polymorphism and mutation databases

BioMutaiTRAK2.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038964 Genomic DNA. Translation: BAB32550.1.
AB038951 mRNA. Translation: BAB32501.1.
AK054763 mRNA. Translation: BAB70803.1.
AC007256 Genomic DNA. No translation available.
AC007282 Genomic DNA. No translation available.
AC080069 Genomic DNA. No translation available.
AB011121 mRNA. Translation: BAA25475.1.
BC017999 mRNA. Translation: AAH17999.1.
CCDSiCCDS2347.1. [O60296-1]
RefSeqiNP_055864.2. NM_015049.2. [O60296-1]
UniGeneiHs.152774.

3D structure databases

ProteinModelPortaliO60296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122452. 29 interactions.
IntActiO60296. 1 interaction.
STRINGi9606.ENSP00000328875.

PTM databases

PhosphoSiteiO60296.

Polymorphism and mutation databases

BioMutaiTRAK2.

Proteomic databases

MaxQBiO60296.
PaxDbiO60296.
PRIDEiO60296.

Protocols and materials databases

DNASUi66008.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332624; ENSP00000328875; ENSG00000115993. [O60296-1]
ENST00000430254; ENSP00000409333; ENSG00000115993. [O60296-2]
GeneIDi66008.
KEGGihsa:66008.
UCSCiuc002uyb.4. human. [O60296-1]

Organism-specific databases

CTDi66008.
GeneCardsiGC02M202241.
H-InvDBHIX0002738.
HGNCiHGNC:13206. TRAK2.
HPAiHPA015827.
MIMi607334. gene.
neXtProtiNX_O60296.
PharmGKBiPA24744.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG246318.
GeneTreeiENSGT00390000003132.
HOGENOMiHOG000143414.
HOVERGENiHBG069248.
InParanoidiO60296.
KOiK15374.
OMAiVGQLKMN.
OrthoDBiEOG7CRTSM.
PhylomeDBiO60296.
TreeFamiTF323495.

Miscellaneous databases

ChiTaRSiTRAK2. human.
GeneWikiiTRAK2.
GenomeRNAii66008.
NextBioi35500578.
PROiO60296.
SOURCEiSearch...

Gene expression databases

BgeeiO60296.
CleanExiHS_TRAK2.
ExpressionAtlasiO60296. baseline and differential.
GenevestigatoriO60296.

Family and domain databases

InterProiIPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
[Graphical view]
PfamiPF04849. HAP1_N. 1 hit.
PF12448. Milton. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2."
    Hadano S., Yanagisawa Y., Skaug J., Fichter K., Nasir J., Martindale D., Koop B.F., Scherer S.W., Nicholson D.W., Rouleau G.A., Ikeda J.-E., Hayden M.R.
    Genomics 71:200-213(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ILE-142 AND ILE-528.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ILE-142.
    Tissue: Cerebellum.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 446-914 (ISOFORM 1).
    Tissue: Brain.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 798-914 (ISOFORM 1), VARIANT ASN-863.
    Tissue: Skin.
  6. "The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking."
    Fransson S., Ruusala A., Aspenstroem P.
    Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RHOT1 AND RHOT2.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTRAK2_HUMAN
AccessioniPrimary (citable) accession number: O60296
Secondary accession number(s): E7EV21
, Q8WVH7, Q96NS2, Q9C0K5, Q9C0K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 25, 2002
Last modified: April 29, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.