Reviewed, UniProtKB/Swiss-Prot O60294 (LCMT2_HUMAN)

Names and origin

Protein names

Recommended name:
    Leucine carboxyl methyltransferase 2
    EC=2.1.1.-
Alternative name(s):
    p21WAF1/CIP1 promoter-interacting protein
    tRNA wybutosine-synthesizing protein 4 homolog
    tRNA yW-synthesizing protein 4 homolog

Gene names

Name:	LCMT2
Synonyms:	KIAA0547, TYW4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA By similarity. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.
Pathway	tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Subunit structure	Interacts with RNF144B/IBRDC2. Ref.6
Sequence similarities	Belongs to the methyltransferase superfamily. LCMT family.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.6 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Leucine carboxyl methyltransferase 2		PRO_0000204423
Regions
	Region	161 – 162	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	89	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity
	Binding site	188	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Natural variations
	Natural variant	67	1	V → L: dbSNP rs45552436. Ref.2		VAR_023378
	Natural variant	141	1	R → S: dbSNP rs3742970. Ref.2		VAR_022081
	Natural variant	149	1	C → Y Ref.2		VAR_023379
	Natural variant	518	1	T → A Ref.2		VAR_023380
Experimental info
	Sequence conflict	164 – 165	2	QL → HV in AAF75774. Ref.4
	Sequence conflict	202	1	L → F in AAF75774. Ref.4
	Sequence conflict	271	1	M → I in BAA25473. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O60294-1 [UniParc]. Last modified May 24, 2004. Version 3. Checksum: 146F1C44F541059F	FASTA	686	75,602
	10 20 30 40 50 60 MGPRSRERRA GAVQNTNDSS ALSKRSLAAR GYVQDPFAAL LVPGAARRAP LIHRGYYVRA 70 80 90 100 110 120 RAVRHCVRAF LEQIGAPQAA LRAQILSLGA GFDSLYFRLK TAGRLARAAV WEVDFPDVAR 130 140 150 160 170 180 RKAERIGETP ELCALTGPFE RGEPASALCF ESADYCILGL DLRQLQRVEE ALGAAGLDAA 190 200 210 220 230 240 SPTLLLAEAV LTYLEPESAA ALIAWAAQRF PNALFVVYEQ MRPQDAFGQF MLQHFRQLNS 250 260 270 280 290 300 PLHGLERFPD VEAQRRRFLQ AGWTACGAVD MNEFYHCFLP AEERRRVENI EPFDEFEEWH 310 320 330 340 350 360 LKCAHYFILA ASRGDTLSHT LVFPSSEAFP RVNPASPSGV FPASVVSSEG QVPNLKRYGH 370 380 390 400 410 420 ASVFLSPDVI LSAGGFGEQE GRHCRVSQFH LLSRDCDSEW KGSQIGSCGT GVQWDGRLYH 430 440 450 460 470 480 TMTRLSESRV LVLGGRLSPV SPALGVLQLH FFKSEDNNTE DLKVTITKAG RKDDSTLCCW 490 500 510 520 530 540 RHSTTEVSCQ NQEYLFVYGG RSVVEPVLSD WHFLHVGTMA WVRIPVEGEV PEARHSHSAC 550 560 570 580 590 600 TWQGGALIAG GLGASEEPLN SVLFLRPISC GFLWESVDIQ PPITPRYSHT AHVLNGKLLL 610 620 630 640 650 660 VGGIWIHSSS FPGVTVINLT TGLSSEYQID TTYVPWPLML HNHTSILLPE EQQLLLLGGG 670 680 GNCFSFGTYF NPHTVTLDLS SLSAGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	NIEHS SNPs program Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-67; SER-141; TYR-149 AND ALA-518.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Isolation of genes coding for p21WAF1/CIP1 promoter-interacting proteins using the yeast one-hybrid system." Li S., Zhang B., Li X. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-686.
[5]	"Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue." De Baere I., Derua R., Janssens V., Van Hoof C., Waelkens E., Merlevede W., Goris J. Biochemistry 38:16539-16547(1999) [PubMed: 10600115] [Abstract] Cited for: FUNCTION.
[6]	"p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1." Ng C.-C., Arakawa H., Fukuda S., Kondoh H., Nakamura Y. Oncogene 22:4449-4458(2003) [PubMed: 12853982] [Abstract] Cited for: INTERACTION WITH RNF144B.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB011119 mRNA. Translation: BAA25473.2. Different initiation. DQ102852 Genomic DNA. Translation: AAY88744.1. BC015949 mRNA. Translation: AAH15949.1. AF265443 mRNA. Translation: AAF75774.1.
IPI	IPI00171712.
RefSeq	NP_055608.2.
UniGene	Hs.200596
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O60294.
Genome annotation databases
Ensembl	ENSG00000168806. Homo sapiens. [Contig view]
GeneID	9836.
KEGG	hsa:9836.
NMPDR	fig|9606.3.peg.10594.
Organism-specific databases
GeneCards	GC15M041407.
H-InvDB	HIX0012182.
HGNC	HGNC:17558. LCMT2.
MIM	611246. gene.
PharmGKB	PA134878443.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60294.
HOVERGEN	O60294.
OMA	O60294. DGRLYHT.
Gene expression databases
ArrayExpress	O60294.
Bgee	O60294.
CleanEx	HS_LCMT2.
GermOnline	ENSG00000168806. Homo sapiens.
Family and domain databases
InterPro	IPR015915. Kelch-typ_b-propeller. IPR011498. Kelch_2. IPR007213. LCM_MeTrfase. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
PANTHER	PTHR13600. LCM_mtfrase. 1 hit.
Pfam	PF07646. Kelch_2. 1 hit. PF04072. LCM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00149. L-Leucine.
NextBio	37062.
SOURCE	Search...

Entry information

Entry name

LCMT2_HUMAN

Accession

Primary (citable) accession number: O60294
Secondary accession number(s): Q4JFT6, Q96B55, Q9NR10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	June 16, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)