O60294 (LCMT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine carboxyl methyltransferase 2
EC=2.1.1.-

Alternative name(s):
p21WAF1/CIP1 promoter-interacting protein
tRNA wybutosine-synthesizing protein 4 homolog
Short name=tRNA yW-synthesizing protein 4 homolog

Gene names

Name:	LCMT2
Synonyms:	KIAA0547, TYW4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA By similarity. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.
Pathway	tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Subunit structure	Interacts with RNF144B/IBRDC2. Ref.5
Sequence similarities	Belongs to the methyltransferase superfamily. LCMT family.
Sequence caution	The sequence BAA25473.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Leucine carboxyl methyltransferase 2		PRO_0000204423
Regions
	Region	161 – 162	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	89	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity
	Binding site	188	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Natural variations
	Natural variant	67	1	V → L. Ref.2 Corresponds to variant rs45552436 [ dbSNP | Ensembl ].		VAR_023378
	Natural variant	141	1	R → S. Ref.2 Corresponds to variant rs3742970 [ dbSNP | Ensembl ].		VAR_022081
	Natural variant	149	1	C → Y. Ref.2 Corresponds to variant rs45593931 [ dbSNP | Ensembl ].		VAR_023379
	Natural variant	518	1	T → A. Ref.2 Corresponds to variant rs45530831 [ dbSNP | Ensembl ].		VAR_023380
Experimental info
	Sequence conflict	164 – 165	2	QL → HV in AAF75774. Ref.4
	Sequence conflict	202	1	L → F in AAF75774. Ref.4
	Sequence conflict	271	1	M → I in BAA25473. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O60294 [UniParc]. Last modified May 24, 2004. Version 3. Checksum: 146F1C44F541059F	FASTA	686	75,602
	10 20 30 40 50 60 MGPRSRERRA GAVQNTNDSS ALSKRSLAAR GYVQDPFAAL LVPGAARRAP LIHRGYYVRA 70 80 90 100 110 120 RAVRHCVRAF LEQIGAPQAA LRAQILSLGA GFDSLYFRLK TAGRLARAAV WEVDFPDVAR 130 140 150 160 170 180 RKAERIGETP ELCALTGPFE RGEPASALCF ESADYCILGL DLRQLQRVEE ALGAAGLDAA 190 200 210 220 230 240 SPTLLLAEAV LTYLEPESAA ALIAWAAQRF PNALFVVYEQ MRPQDAFGQF MLQHFRQLNS 250 260 270 280 290 300 PLHGLERFPD VEAQRRRFLQ AGWTACGAVD MNEFYHCFLP AEERRRVENI EPFDEFEEWH 310 320 330 340 350 360 LKCAHYFILA ASRGDTLSHT LVFPSSEAFP RVNPASPSGV FPASVVSSEG QVPNLKRYGH 370 380 390 400 410 420 ASVFLSPDVI LSAGGFGEQE GRHCRVSQFH LLSRDCDSEW KGSQIGSCGT GVQWDGRLYH 430 440 450 460 470 480 TMTRLSESRV LVLGGRLSPV SPALGVLQLH FFKSEDNNTE DLKVTITKAG RKDDSTLCCW 490 500 510 520 530 540 RHSTTEVSCQ NQEYLFVYGG RSVVEPVLSD WHFLHVGTMA WVRIPVEGEV PEARHSHSAC 550 560 570 580 590 600 TWQGGALIAG GLGASEEPLN SVLFLRPISC GFLWESVDIQ PPITPRYSHT AHVLNGKLLL 610 620 630 640 650 660 VGGIWIHSSS FPGVTVINLT TGLSSEYQID TTYVPWPLML HNHTSILLPE EQQLLLLGGG 670 680 GNCFSFGTYF NPHTVTLDLS SLSAGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	NIEHS SNPs program Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-67; SER-141; TYR-149 AND ALA-518.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Isolation of genes coding for p21WAF1/CIP1 promoter-interacting proteins using the yeast one-hybrid system." Li S., Zhang B., Li X. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-686.
[5]	"p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1." Ng C.-C., Arakawa H., Fukuda S., Kondoh H., Nakamura Y. Oncogene 22:4449-4458(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RNF144B.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011119 mRNA. Translation: BAA25473.2. Different initiation. DQ102852 Genomic DNA. Translation: AAY88744.1. BC015949 mRNA. Translation: AAH15949.1. AF265443 mRNA. Translation: AAF75774.1.
IPI	IPI00171712.
RefSeq	NP_055608.2. NM_014793.4.
UniGene	Hs.200596. Hs.533913. Hs.741946.
3D structure databases
ProteinModelPortal	O60294.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000307214.
PTM databases
PhosphoSite	O60294.
Proteomic databases
PaxDb	O60294.
PRIDE	O60294.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305641; ENSP00000307214; ENSG00000168806.
GeneID	9836.
KEGG	hsa:9836.
UCSC	uc001zrg.3. human.
Organism-specific databases
CTD	9836.
GeneCards	GC15M043619.
HGNC	HGNC:17558. LCMT2.
HPA	HPA039401.
MIM	611246. gene.
neXtProt	NX_O60294.
PharmGKB	PA134878443.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG329644.
HOGENOM	HOG000231851.
HOVERGEN	HBG052314.
InParanoid	O60294.
KO	K15451.
OMA	HTMTRLS.
OrthoDB	EOG46Q6S2.
Enzyme and pathway databases
UniPathway	UPA00375.
Gene expression databases
ArrayExpress	O60294.
Bgee	O60294.
CleanEx	HS_LCMT2.
Genevestigator	O60294.
GermOnline	ENSG00000168806. Homo sapiens.
Family and domain databases
Gene3D	2.120.10.80. 1 hit.
InterPro	IPR015915. Kelch-typ_b-propeller. IPR007213. LCM_MeTrfase. IPR021121. LCM_MeTrfase_euk. [Graphical view]
PANTHER	PTHR13600. PTHR13600. 1 hit.
Pfam	PF04072. LCM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00149. L-Leucine.
GenomeRNAi	9836.
NextBio	37062.
SOURCE	Search...

Entry information

Entry name

LCMT2_HUMAN

Accession

Primary (citable) accession number: O60294
Secondary accession number(s): Q4JFT6, Q96B55, Q9NR10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	May 1, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.