O60282 (KIF5C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin heavy chain isoform 5C

Alternative name(s):
Kinesin heavy chain neuron-specific 2

Gene names

Name:	KIF5C
Synonyms:	KIAA0531, NKHC2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	957 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates dendritic trafficking of mRNAs By similarity. Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Subunit structure	Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and KLC3 By similarity. Interacts with TRAK1. Ref.5
Subcellular location	Cytoplasm › cytoskeleton Probable.
Tissue specificity	Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
Domain	Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain.
Sequence caution	The sequence BAA25457.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA transport Inferred from sequence or structural similarity. Source: UniProtKB microtubule-based movement Inferred from electronic annotation. Source: InterPro motor neuron axon guidance Inferred from electronic annotation. Source: Compara organelle organization Traceable author statement PubMed 9782088. Source: ProtInc
Cellular_component	ciliary rootlet Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW kinesin complex Traceable author statement PubMed 9782088. Source: ProtInc microtubule Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Traceable author statement PubMed 9782088. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CSNK2A1	P68400	4	EBI-717170,EBI-347804
CSNK2A2	P19784	4	EBI-717170,EBI-347451
RHOT1	Q8IXI2	2	EBI-717170,EBI-1396430

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60282-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60282-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.
     233-238: LAGSEK → MATYIH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 957	956	Kinesin heavy chain isoform 5C		PRO_0000125355
Regions
	Domain	2 – 386	385	Kinesin-motor
	Nucleotide binding	86 – 93	8	ATP By similarity
	Region	174 – 315	142	Microtubule-binding
	Region	859 – 956	98	Globular
	Coiled coil	406 – 923	518
Amino acid modifications
	Modified residue	338	1	Phosphothreonine By similarity
	Modified residue	797	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 232	232	Missing in isoform 2.		VSP_035715
	Alternative sequence	233 – 238	6	LAGSEK → MATYIH in isoform 2.		VSP_035716
Experimental info
	Sequence conflict	355 – 360	6	TLKNVI → STHASV in AAD01436. Ref.4
	Sequence conflict	583 – 585	3	EFT → DRV in AAD01436. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A9F25BB1C994322A	FASTA	957	109,495
	10 20 30 40 50 60 MADPAECSIK VMCRFRPLNE AEILRGDKFI PKFKGDETVV IGQGKPYVFD RVLPPNTTQE 70 80 90 100 110 120 QVYNACAKQI VKDVLEGYNG TIFAYGQTSS GKTHTMEGKL HDPQLMGIIP RIAHDIFDHI 130 140 150 160 170 180 YSMDENLEFH IKVSYFEIYL DKIRDLLDVS KTNLAVHEDK NRVPYVKGCT ERFVSSPEEV 190 200 210 220 230 240 MDVIDEGKAN RHVAVTNMNE HSSRSHSIFL INIKQENVET EKKLSGKLYL VDLAGSEKVS 250 260 270 280 290 300 KTGAEGAVLD EAKNINKSLS ALGNVISALA EGTKTHVPYR DSKMTRILQD SLGGNCRTTI 310 320 330 340 350 360 VICCSPSVFN EAETKSTLMF GQRAKTIKNT VSVNLELTAE EWKKKYEKEK EKNKTLKNVI 370 380 390 400 410 420 QHLEMELNRW RNGEAVPEDE QISAKDQKNL EPCDNTPIID NIAPVVAGIS TEEKEKYDEE 430 440 450 460 470 480 ISSLYRQLDD KDDEINQQSQ LAEKLKQQML DQDELLASTR RDYEKIQEEL TRLQIENEAA 490 500 510 520 530 540 KDEVKEVLQA LEELAVNYDQ KSQEVEDKTR ANEQLTDELA QKTTTLTTTQ RELSQLQELS 550 560 570 580 590 600 NHQKKRATEI LNLLLKDLGE IGGIIGTNDV KTLADVNGVI EEEFTMARLY ISKMKSEVKS 610 620 630 640 650 660 LVNRSKQLES AQMDSNRKMN ASERELAACQ LLISQHEAKI KSLTDYMQNM EQKRRQLEES 670 680 690 700 710 720 QDSLSEELAK LRAQEKMHEV SFQDKEKEHL TRLQDAEEMK KALEQQMESH REAHQKQLSR 730 740 750 760 770 780 LRDEIEEKQK IIDEIRDLNQ KLQLEQEKLS SDYNKLKIED QEREMKLEKL LLLNDKREQA 790 800 810 820 830 840 REDLKGLEET VSRELQTLHN LRKLFVQDLT TRVKKSVELD NDDGGGSAAQ KQKISFLENN 850 860 870 880 890 900 LEQLTKVHKQ LVRDNADLRC ELPKLEKRLR ATAERVKALE SALKEAKENA MRDRKRYQQE 910 920 930 940 950 VDRIKEAVRA KNMARRAHSA QIAKPIRPGH YPASSPTAVH AIRGGGGSSS NSTHYQK
	Isoform 2 [UniParc]. Checksum: 323C36FF4653E281	FASTA	725	83,178
	10 20 30 40 50 60 MATYIHVSKT GAEGAVLDEA KNINKSLSAL GNVISALAEG TKTHVPYRDS KMTRILQDSL 70 80 90 100 110 120 GGNCRTTIVI CCSPSVFNEA ETKSTLMFGQ RAKTIKNTVS VNLELTAEEW KKKYEKEKEK 130 140 150 160 170 180 NKTLKNVIQH LEMELNRWRN GEAVPEDEQI SAKDQKNLEP CDNTPIIDNI APVVAGISTE 190 200 210 220 230 240 EKEKYDEEIS SLYRQLDDKD DEINQQSQLA EKLKQQMLDQ DELLASTRRD YEKIQEELTR 250 260 270 280 290 300 LQIENEAAKD EVKEVLQALE ELAVNYDQKS QEVEDKTRAN EQLTDELAQK TTTLTTTQRE 310 320 330 340 350 360 LSQLQELSNH QKKRATEILN LLLKDLGEIG GIIGTNDVKT LADVNGVIEE EFTMARLYIS 370 380 390 400 410 420 KMKSEVKSLV NRSKQLESAQ MDSNRKMNAS ERELAACQLL ISQHEAKIKS LTDYMQNMEQ 430 440 450 460 470 480 KRRQLEESQD SLSEELAKLR AQEKMHEVSF QDKEKEHLTR LQDAEEMKKA LEQQMESHRE 490 500 510 520 530 540 AHQKQLSRLR DEIEEKQKII DEIRDLNQKL QLEQEKLSSD YNKLKIEDQE REMKLEKLLL 550 560 570 580 590 600 LNDKREQARE DLKGLEETVS RELQTLHNLR KLFVQDLTTR VKKSVELDND DGGGSAAQKQ 610 620 630 640 650 660 KISFLENNLE QLTKVHKQLV RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENAMR 670 680 690 700 710 720 DRKRYQQEVD RIKEAVRAKN MARRAHSAQI AKPIRPGHYP ASSPTAVHAI RGGGGSSSNS THYQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued subunit of dynactin." Engelender S., Sharp A.H., Colomer V., Tokito M.K., Lanahan A., Worley P., Holzbaur E.L.F., Ross C.A. Hum. Mol. Genet. 6:2205-2212(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 355-585 (ISOFORMS 1/2).
[5]	"GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin." Brickley K., Smith M.J., Beck M., Stephenson F.A. J. Biol. Chem. 280:14723-14732(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TRAK1.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011103 mRNA. Translation: BAA25457.2. Different initiation. AC105402 Genomic DNA. No translation available. AC108512 Genomic DNA. No translation available. AC144443 Genomic DNA. No translation available. AC144611 Genomic DNA. No translation available. BC110287 mRNA. Translation: AAI10288.1. AF010146 mRNA. Translation: AAD01436.1.
IPI	IPI00028561. IPI00889664.
RefSeq	NP_004513.1. NM_004522.2.
UniGene	Hs.435557. Hs.660699.
3D structure databases
ProteinModelPortal	O60282.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60282. 7 interactions.
STRING	9606.ENSP00000393379.
PTM databases
PhosphoSite	O60282.
Proteomic databases
PaxDb	O60282.
PRIDE	O60282.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000397413; ENSP00000380560; ENSG00000168280. ENST00000435030; ENSP00000393379; ENSG00000168280. ENST00000574197; ENSP00000460677; ENSG00000262907. ENST00000576072; ENSP00000461857; ENSG00000262907.
GeneID	3800.
KEGG	hsa:3800.
UCSC	uc010zbu.2. human.
Organism-specific databases
CTD	3800.
GeneCards	GC02P149632.
HGNC	HGNC:6325. KIF5C.
HPA	HPA035210.
MIM	604593. gene.
neXtProt	NX_O60282.
PharmGKB	PA30109.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5059.
HOGENOM	HOG000216718.
HOVERGEN	HBG006210.
InParanoid	O60282.
KO	K10396.
OMA	GQGKPYV.
Gene expression databases
ArrayExpress	O60282.
Bgee	O60282.
CleanEx	HS_KIF5C.
Genevestigator	O60282.
GermOnline	ENSG00000168280. Homo sapiens.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	KIF5C. human.
GenomeRNAi	3800.
NextBio	14921.
SOURCE	Search...

Entry information

Entry name

KIF5C_HUMAN

Accession

Primary (citable) accession number: O60282
Secondary accession number(s): O95079, Q2YDC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.