O60271 (JIP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-Jun-amino-terminal kinase-interacting protein 4
Short name=JIP-4
Short name=JNK-interacting protein 4

Alternative name(s):
Cancer/testis antigen 89
Short name=CT89
Human lung cancer oncogene 6 protein
Short name=HLC-6
JNK-associated leucine-zipper protein
Short name=JLP
Mitogen-activated protein kinase 8-interacting protein 4
Proliferation-inducing protein 6
Protein highly expressed in testis
Short name=PHET
Sperm surface protein
Sperm-associated antigen 9
Sperm-specific protein
Sunday driver 1

Gene names

Name:	SPAG9
Synonyms:	HSS, KIAA0516, MAPK8IP4, SYD1
ORF Names:	HLC6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction. Ref.11 Ref.12
Subunit structure	Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Ref.11 Ref.12
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. Ref.9 Ref.12 Isoform 5: Cytoplasmic vesicle › secretory vesicle › acrosome. Note: Associated with the plasma membrane of the acrosomal compartment and also localizes in the acrosome matrix. Ref.9 Ref.12
Tissue specificity	Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Ref.2 Ref.8 Ref.9
Induction	Isoform 3 is increased in systemic sclerosis fibroblasts. Ref.9
Post-translational modification	Phosphorylated by MAPK8 and MAPK14 By similarity.
Sequence similarities	Belongs to the JIP scaffold family.
Sequence caution	The sequence AAH07524.1 differs from that shown. Reason: Probable cloning artifact. The sequence AAH59946.1 differs from that shown. Reason: Probable cloning artifact. The sequence AAI06049.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the C-terminal part. The sequence AAO66462.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA25442.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB14812.1 differs from that shown. Reason: Unlikely isoform. Aberrant splicing. The sequence CAA62987.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of JUN kinase activity Inferred from electronic annotation. Source: Compara muscle cell differentiation Traceable author statement. Source: Reactome negative regulation of protein homodimerization activity Inferred from electronic annotation. Source: Compara positive regulation of cell migration Inferred from mutant phenotype PubMed 18826971. Source: UniProtKB positive regulation of muscle cell differentiation Traceable author statement. Source: Reactome positive regulation of neuron differentiation Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara retrograde transport, endosome to Golgi Inferred from direct assay PubMed 19056739. Source: MGI spermatogenesis Traceable author statement Ref.8. Source: ProtInc striated muscle cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome integral to membrane Traceable author statement Ref.8. Source: ProtInc perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60271-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60271-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     555-555: F → FVPTR

Isoform 3 (identifier: O60271-3)

The sequence of this isoform differs from the canonical sequence as follows:
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Note: Due to intron retention.

Isoform 4 (identifier: O60271-4)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.

Isoform 5 (identifier: O60271-5)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Isoform 6 (identifier: O60271-9)

The sequence of this isoform differs from the canonical sequence as follows:
     1-196: MELEDGVVYQ...QLESTAHSRI → MSPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     1175-1175: T → TVILHQGRLLGLRA

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1321

1321

C-Jun-amino-terminal kinase-interacting protein 4

PRO_0000234076

Regions

Coiled coil

66 – 166

101

Potential

Coiled coil

408 – 534

127

Potential

Coiled coil

724 – 758

35

Potential

Amino acid modifications

Modified residue

109

1

Phosphoserine Ref.14

Modified residue

183

1

Phosphoserine Ref.16 Ref.18 Ref.20

Modified residue

185

1

Phosphoserine Ref.16 Ref.20

Modified residue

194

1

Phosphoserine Ref.18

Modified residue

203

1

Phosphoserine Ref.13 Ref.17 Ref.18

Modified residue

217

1

Phosphothreonine Ref.13 Ref.16 Ref.17 Ref.18

Modified residue

244

1

Phosphothreonine By similarity

Modified residue

251

1

Phosphoserine Ref.18

Modified residue

265

1

Phosphoserine Ref.14 Ref.18

Modified residue

268

1

Phosphoserine Ref.18

Modified residue

272

1

Phosphoserine Ref.14 Ref.18

Modified residue

292

1

Phosphothreonine By similarity

Modified residue

311

1

Phosphoserine Ref.16

Modified residue

329

1

Phosphoserine Ref.16 Ref.18 Ref.20

Modified residue

332

1

Phosphoserine Ref.16 Ref.18 Ref.20

Modified residue

347

1

Phosphoserine Ref.18

Modified residue

348

1

Phosphothreonine Ref.18

Modified residue

365

1

Phosphothreonine By similarity

Modified residue

418

1

Phosphothreonine Ref.14 Ref.16 Ref.18

Modified residue

586

1

Phosphothreonine Ref.16

Modified residue

730

1

Phosphoserine Ref.18

Modified residue

733

1

Phosphoserine Ref.16 Ref.17 Ref.18

Natural variations

Alternative sequence

1 – 196

196

MELED…AHSRI → MSPGCMLLFVFGFVGGAVVI NSAILVSLSVLLLVHFSIST GVPALTQNLPRIL in isoform 6.

VSP_042253

Alternative sequence

248 – 261

14

Missing in isoform 2, isoform 4, isoform 5 and isoform 6.

VSP_018214

Alternative sequence

555

1

F → FVPTR in isoform 2.

VSP_018220

Alternative sequence

938 – 945

8

SNDSDAYK → RYNNGSST in isoform 3 and isoform 5.

VSP_018221

Alternative sequence

946 – 1321

376

Missing in isoform 3 and isoform 5.

VSP_018222

Alternative sequence

1175

1

T → TVILHQGRLLGLRA in isoform 6.

VSP_042254

Natural variant

1320

1

N → S.
Corresponds to variant rs9896965 [ dbSNP | Ensembl ].

VAR_059364

Experimental info

Sequence conflict

451

1

E → G in AAO66462. Ref.9

Sequence conflict

680

1

E → K in CAA62987. Ref.8

Sequence conflict

680

1

E → K in AAO66462. Ref.9

Isoform 6:

Sequence conflict

9

1

F → S in BAG58134. Ref.5

Secondary structure

1

.

.

.

1321

Helix Strand Turn

Details...

Helix

406 – 465

60

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 4. Checksum: 5CAE349FBDF91B40	FASTA	1,321	146,205
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKVSN SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 310 320 330 340 350 360 FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE LDMDKDLSGY 370 380 390 400 410 420 KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 430 440 450 460 470 480 LETKNALNIV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEEKNRELE EELRKARAEA 490 500 510 520 530 540 EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 550 560 570 580 590 600 NPAMQEKKRS SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 610 620 630 640 650 660 GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG 670 680 690 700 710 720 QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD 730 740 750 760 770 780 TEGSKQRSAS QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN 790 800 810 820 830 840 ILDSFTVCNS HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG 850 860 870 880 890 900 GITVVGCSAE GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 910 920 930 940 950 960 SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV LPNEQDLVRE 970 980 990 1000 1010 1020 EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG 1030 1040 1050 1060 1070 1080 TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK 1090 1100 1110 1120 1130 1140 SFDAHPRKES QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK 1150 1160 1170 1180 1190 1200 LGFSFVRITA LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK 1210 1220 1230 1240 1250 1260 VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG DKAGPSAQEP 1270 1280 1290 1300 1310 1320 GSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMYGN E
	Isoform 2 [UniParc]. Checksum: D60A544D70BD1F97	FASTA	1,311	145,135
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FVPTRFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP GDKSKAFDFL 610 620 630 640 650 660 SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG QGENKMKNLP 670 680 690 700 710 720 VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD TEGSKQRSAS 730 740 750 760 770 780 QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN ILDSFTVCNS 790 800 810 820 830 840 HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG GITVVGCSAE 850 860 870 880 890 900 GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG SAEDTVDISQ 910 920 930 940 950 960 TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV LPNEQDLVRE EAQKMSSLLP 970 980 990 1000 1010 1020 TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG TLAIFHRGVD 1030 1040 1050 1060 1070 1080 GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK SFDAHPRKES 1090 1100 1110 1120 1130 1140 QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK LGFSFVRITA 1150 1160 1170 1180 1190 1200 LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK VTPGTFIPYC 1210 1220 1230 1240 1250 1260 SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG DKAGPSAQEP GSQTPLKSML 1270 1280 1290 1300 1310 VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMYGN E
	Isoform 3 [UniParc]. Checksum: EB17642AA2BF0BB4	FASTA	945	104,736
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKVSN SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 310 320 330 340 350 360 FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE LDMDKDLSGY 370 380 390 400 410 420 KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 430 440 450 460 470 480 LETKNALNIV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEEKNRELE EELRKARAEA 490 500 510 520 530 540 EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 550 560 570 580 590 600 NPAMQEKKRS SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 610 620 630 640 650 660 GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG 670 680 690 700 710 720 QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD 730 740 750 760 770 780 TEGSKQRSAS QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN 790 800 810 820 830 840 ILDSFTVCNS HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG 850 860 870 880 890 900 GITVVGCSAE GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 910 920 930 940 SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSRYN NGSST
	Isoform 4 [UniParc]. Checksum: 55B30E422A8ABD0E	FASTA	1,307	144,682
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NTTKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVTNGQGEN KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDTS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DVAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKELKNQEEL SSLVWICTST HSATKVLIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEDLSE SGQVDKASLC GSMTSNSSAE TDSLLGGITV VGCSAEGVTG 850 860 870 880 890 900 AATSPSTNGA SPVMDKPPEM EAENSEVDEN VPTAEEATEA TEGNAGSAED TVDISQTGVY 910 920 930 940 950 960 TEHVFTDPLG VQIPEDLSPV YQSSNDSDAY KDQISVLPNE QDLVREEAQK MSSLLPTMWL 970 980 990 1000 1010 1020 GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD 1030 1040 1050 1060 1070 1080 LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ 1090 1100 1110 1120 1130 1140 LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS 1150 1160 1170 1180 1190 1200 CNRLWVGTGN GVIISIPLTE TNKTSGVPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH 1210 1220 1230 1240 1250 1260 AQLCFHGHRD AVKFFVAVPG QVISPQSSSS GTDLTGDKAG PSAQEPGSQT PLKSMLVISG 1270 1280 1290 1300 GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMYGNE
	Isoform 5 [UniParc]. Checksum: 1A427D7B60367814	FASTA	931	103,212
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NTTKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVTNGQGEN KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDTS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DVAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKELKNQEEL SSLVWICTST HSATKVLIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEDLSE SGQVDKASLC GSMTSNSSAE TDSLLGGITV VGCSAEGVTG 850 860 870 880 890 900 AATSPSTNGA SPVMDKPPEM EAENSEVDEN VPTAEEATEA TEGNAGSAED TVDISQTGVY 910 920 930 TEHVFTDPLG VQIPEDLSPV YQSRYNNGSS T
	Isoform 6 [UniParc]. Checksum: 8CD074A547DCC690	FASTA	1,177	128,607
	10 20 30 40 50 60 MSPGCMLLFV FGFVGGAVVI NSAILVSLSV LLLVHFSIST GVPALTQNLP RILRKERPIS 70 80 90 100 110 120 LGIFPLPAGD GLLTPDAQKG GETPGSEQWK FQELSQPRSH TSLKDELSDV SQGGSKATTP 130 140 150 160 170 180 ASTANSDVAT IPTDTPLKEE NEGFVKVTDA PNKSEISKHI EVQVAQETRN VSTGSAENEE 190 200 210 220 230 240 KSEVQAIIES TPELDMDKDL SGYKGSSTPT KGIENKAFDR NTESLFEELS SAGSGLIGDV 250 260 270 280 290 300 DEGADLLGMG REVENLILEN TQLLETKNAL NIVKNDLIAK VDELTCEKDV LQGELEAVKQ 310 320 330 340 350 360 AKLKLEEKNR ELEEELRKAR AEAEDARQKA KDDDDSDIPT AQRKRFTRVE MARVLMERNQ 370 380 390 400 410 420 YKERLMELQE AVRWTEMIRA SRENPAMQEK KRSSIWQFFS RLFSSSSNTT KKPEPPVNLK 430 440 450 460 470 480 YNAPTSHVTP SVKKRSSTLS QLPGDKSKAF DFLSEETEAS LASRREQKRE QYRQVKAHVQ 490 500 510 520 530 540 KEDGRVQAFG WSLPQKYKQV TNGQGENKMK NLPVPVYLRP LDEKDTSMKL WCAVGVNLSG 550 560 570 580 590 600 GKTRDGGSVV GASVFYKDVA GLDTEGSKQR SASQSSLDKL DQELKEQQKE LKNQEELSSL 610 620 630 640 650 660 VWICTSTHSA TKVLIIDAVQ PGNILDSFTV CNSHVLCIAS VPGARETDYP AGEDLSESGQ 670 680 690 700 710 720 VDKASLCGSM TSNSSAETDS LLGGITVVGC SAEGVTGAAT SPSTNGASPV MDKPPEMEAE 730 740 750 760 770 780 NSEVDENVPT AEEATEATEG NAGSAEDTVD ISQTGVYTEH VFTDPLGVQI PEDLSPVYQS 790 800 810 820 830 840 SNDSDAYKDQ ISVLPNEQDL VREEAQKMSS LLPTMWLGAQ NGCLYVHSSV AQWRKCLHSI 850 860 870 880 890 900 KLKDSILSIV HVKGIVLVAL ADGTLAIFHR GVDGQWDLSN YHLLDLGRPH HSIRCMTVVH 910 920 930 940 950 960 DKVWCGYRNK IYVVQPKAMK IEKSFDAHPR KESQVRQLAW VGDGVWVSIR LDSTLRLYHA 970 980 990 1000 1010 1020 HTYQHLQDVD IEPYVSKMLG TGKLGFSFVR ITALMVSCNR LWVGTGNGVI ISIPLTETVI 1030 1040 1050 1060 1070 1080 LHQGRLLGLR ANKTSGVPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH AQLCFHGHRD 1090 1100 1110 1120 1130 1140 AVKFFVAVPG QVISPQSSSS GTDLTGDKAG PSAQEPGSQT PLKSMLVISG GEGYIDFRMG 1150 1160 1170 DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMYGNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors." Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P. Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Liver.
[2]	"Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia." Guinn B.-A., Bland E.A., Lodi U., Liggins A.P., Tobal K., Petters S., Wells J.W., Banham A.H., Mufti G.J. Biochem. Biophys. Res. Commun. 335:1293-1304(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY.
[3]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6). Tissue: Brain and Testis.
[6]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-478 (ISOFORMS 2/4/5). Tissue: Eye and Kidney.
[8]	"Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif." Shankar S., Mohapatra B., Suri A. Biochem. Biophys. Res. Commun. 243:561-565(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 130-1321 (ISOFORM 5), TISSUE SPECIFICITY. Tissue: Testis.
[9]	"A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies." Yasuoka H., Ihn H., Medsger T.A. Jr., Hirakata M., Kawakami Y., Ikeda Y., Kuwana M. J. Immunol. 171:6883-6890(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 162-1321 (ISOFORM 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION. Tissue: Testis.
[10]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[11]	"A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway." Bouwmeester T., Bauch A., Ruffner H., Angrand P.-O., Bergamini G., Croughton K., Cruciat C., Eberhard D., Gagneur J., Ghidelli S., Hopf C., Huhse B., Mangano R., Michon A.-M., Schirle M., Schlegl J., Schwab M., Stein M.A. , Bauer A., Casari G., Drewes G., Gavin A.-C., Jackson D.B., Joberty G., Neubauer G., Rick J., Kuster B., Superti-Furga G. Nat. Cell Biol. 6:97-105(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NFKB1.
[12]	"Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein." Jagadish N., Rana R., Selvi R., Mishra D., Garg M., Yadav S., Herr J.C., Okumura K., Hasegawa A., Koyama K., Suri A. Biochem. J. 389:73-82(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAPK9; MAPK10 AND MAPK8, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[13]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203 AND THR-217, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109; SER-265; SER-272 AND THR-418, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[15]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[16]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-185; THR-217; SER-311; SER-329; SER-332; THR-418; THR-586 AND SER-733, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; THR-217 AND SER-733, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[18]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-194; SER-203; THR-217; SER-251; SER-265; SER-268; SER-272; SER-329; SER-332; SER-347; THR-348; THR-418; SER-730 AND SER-733, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[19]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[20]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-185; SER-329 AND SER-332, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF327452 mRNA. Translation: AAN61565.1.
AY850123 mRNA. Translation: AAX47276.1.
AB011088 mRNA. Translation: BAA25442.3. Different initiation.
AK024068 mRNA. Translation: BAB14812.1. Sequence problems.
AK295098 mRNA. Translation: BAG58134.1.
AK302789 mRNA. Translation: BAG63993.1.
AC005920 Genomic DNA. No translation available.
AC005839 Genomic DNA. No translation available.
BC007524 mRNA. Translation: AAH07524.1. Sequence problems.
BC059946 mRNA. Translation: AAH59946.1. Sequence problems.
BC106048 mRNA. Translation: AAI06049.1. Sequence problems.
BC146755 mRNA. Translation: AAI46756.1.
BC153878 mRNA. Translation: AAI53879.1.
X91879 mRNA. Translation: CAA62987.1. Different initiation.
AY219897 mRNA. Translation: AAO66462.1. Different initiation.
AY219898 mRNA. Translation: AAO66463.1.

IPI

IPI00303397.
IPI00384432.
IPI00479676.
IPI00744288.
IPI00744648.
IPI00748244.
IPI00748833.
IPI00879552.
IPI01011459.

PIR

JC5958.

RefSeq

NP_001123999.1. NM_001130527.2.
NP_001124000.1. NM_001130528.2.
NP_001238900.1. NM_001251971.1.
NP_003962.3. NM_003971.5.

UniGene

Hs.463439.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2W83	X-ray	1.93	C/D	406-476	[»]

ProteinModelPortal

O60271.

ModBase

Search...

Protein-protein interaction databases

IntAct

O60271. 5 interactions.

MINT

MINT-1136008.

PTM databases

PhosphoSite

O60271.

Proteomic databases

PaxDb

O60271.

PRIDE

O60271.

Protocols and materials databases

DNASU

9043.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000262013; ENSP00000262013; ENSG00000008294.
ENST00000357122; ENSP00000349636; ENSG00000008294.
ENST00000505279; ENSP00000426900; ENSG00000008294.
ENST00000510283; ENSP00000423165; ENSG00000008294.

GeneID

9043.

KEGG

hsa:9043.

UCSC

uc002itb.3. human.
uc002itc.3. human.
uc002itd.3. human.
uc002ite.3. human.
uc002itf.3. human.

Organism-specific databases

CTD

9043.

GeneCards

GC17M049039.

HGNC

HGNC:14524. SPAG9.

HPA

HPA040446.

MIM

605430. gene.

neXtProt

NX_O60271.

PharmGKB

PA37890.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG270333.

HOGENOM

HOG000290716.

HOVERGEN

HBG024110.

InParanoid

O60271.

OMA

KKLWCAV.

OrthoDB

EOG4QJRMF.

Enzyme and pathway databases

Reactome

REACT_111045. Developmental Biology.

Gene expression databases

ArrayExpress

O60271.

Bgee

O60271.

Genevestigator

O60271.

GermOnline

ENSG00000008294. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 1 hit.

InterPro

IPR019143. JNK/Rab-associated_protein-1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF09744. Jnk-SapK_ap_N. 1 hit.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

SPAG9. human.

EvolutionaryTrace

O60271.

GenomeRNAi

9043.

NextBio

33871.

PMAP-CutDB

O60271.

SOURCE

Search...

Entry information

Entry name

JIP4_HUMAN

Accession

Primary (citable) accession number: O60271
Secondary accession number(s): A6H8U5 , A8MSX0, B4DHH2, O60905, Q3KQU8, Q3MKM7, Q86WC7, Q86WC8, Q8IZX7, Q96II0, Q9H811

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.