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UniProtKB - O60271 (JIP4_HUMAN)

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Protein

C-Jun-amino-terminal kinase-interacting protein 4

Gene

SPAG9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.2 Publications

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-375170. CDO in myogenesis.
SignaLinkiO60271.
SIGNORiO60271.

Names & Taxonomyi

Protein namesi
Recommended name:
C-Jun-amino-terminal kinase-interacting protein 4
Short name:
JIP-4
Short name:
JNK-interacting protein 4
Alternative name(s):
Cancer/testis antigen 89
Short name:
CT89
Human lung cancer oncogene 6 protein
Short name:
HLC-6
JNK-associated leucine-zipper protein
Short name:
JLP
Mitogen-activated protein kinase 8-interacting protein 4
Proliferation-inducing protein 6
Protein highly expressed in testis
Short name:
PHET
Sperm surface protein
Sperm-associated antigen 9
Sperm-specific protein
Sunday driver 1
Gene namesi
Name:SPAG9
Synonyms:HSS, KIAA0516, MAPK8IP4, SYD1
ORF Names:HLC6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:14524. SPAG9.

Subcellular locationi

Isoform 5 :

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB-SubCell
  • cytoplasm Source: GO_Central
  • cytosol Source: HPA
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • microtubule organizing center Source: HPA
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Organism-specific databases

DisGeNETi9043.
OpenTargetsiENSG00000008294.
PharmGKBiPA37890.

Polymorphism and mutation databases

BioMutaiSPAG9.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340761 – 1321C-Jun-amino-terminal kinase-interacting protein 4Add BLAST1321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei109PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei194PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1
Modified residuei217PhosphothreonineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei251PhosphoserineCombined sources1
Modified residuei265PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1
Modified residuei292PhosphothreonineBy similarity1
Modified residuei311PhosphoserineCombined sources1
Modified residuei329PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1
Modified residuei347PhosphoserineCombined sources1
Modified residuei348PhosphothreonineCombined sources1
Modified residuei365PhosphothreonineBy similarity1
Modified residuei418PhosphothreonineCombined sources1
Modified residuei586PhosphothreonineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei595PhosphothreonineCombined sources1
Modified residuei705PhosphoserineCombined sources1
Modified residuei728PhosphoserineCombined sources1
Modified residuei730PhosphoserineCombined sources1
Modified residuei732PhosphoserineCombined sources1
Modified residuei733PhosphoserineCombined sources1
Modified residuei1188PhosphoserineCombined sources1
Modified residuei1264PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by MAPK8 and MAPK14.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60271.
MaxQBiO60271.
PaxDbiO60271.
PeptideAtlasiO60271.
PRIDEiO60271.

PTM databases

iPTMnetiO60271.
PhosphoSitePlusiO60271.

Miscellaneous databases

PMAP-CutDBiO60271.

Expressioni

Tissue specificityi

Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.3 Publications

Inductioni

Isoform 3 is increased in systemic sclerosis fibroblasts.1 Publication

Gene expression databases

BgeeiENSG00000008294.
ExpressionAtlasiO60271. baseline and differential.
GenevisibleiO60271. HS.

Organism-specific databases

HPAiHPA040446.
HPA061458.

Interactioni

Subunit structurei

Homodimer. The homodimer interacts with ARF6, forming a heterotetramer. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

BioGridi114505. 59 interactors.
IntActiO60271. 12 interactors.
MINTiMINT-1136008.
STRINGi9606.ENSP00000262013.

Structurei

Secondary structure

11321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi406 – 465Combined sources60

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W83X-ray1.93C/D406-476[»]
ProteinModelPortaliO60271.
SMRiO60271.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60271.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 95RH1PROSITE-ProRule annotationAdd BLAST89
Domaini500 – 571RH2PROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 166Sequence analysisAdd BLAST101
Coiled coili408 – 5341 PublicationAdd BLAST127
Coiled coili724 – 758Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the JIP scaffold family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2077. Eukaryota.
ENOG410XQ19. LUCA.
GeneTreeiENSGT00670000097546.
HOGENOMiHOG000290716.
HOVERGENiHBG024110.
InParanoidiO60271.
KOiK20317.
OMAiRENPAMQ.
OrthoDBiEOG091G016S.
PhylomeDBiO60271.
TreeFamiTF313096.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR032486. JIP_LZII.
IPR019143. JNK/Rab-associated_protein-1_N.
IPR034743. RH1.
IPR034744. RH2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF16471. JIP_LZII. 1 hit.
PF09744. Jnk-SapK_ap_N. 1 hit.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS51776. RH1. 1 hit.
PS51777. RH2. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60271-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL 
        60         70         80         90        100
MPLVVAVLEN LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE 
       110        120        130        140        150
EKFIEFEDSQ EQEKKDLQTR VESLESQTRQ LELKAKNYAD QISRLEEREA 
       160        170        180        190        200
ELKKEYNALH QRHTEMIHNY MEHLERTKLH QLSGSDQLES TAHSRIRKER 
       210        220        230        240        250
PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP RSHTSLKVSN 
       260        270        280        290        300
SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 
       310        320        330        340        350
FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE 
       360        370        380        390        400
LDMDKDLSGY KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG 
       410        420        430        440        450
ADLLGMGREV ENLILENTQL LETKNALNIV KNDLIAKVDE LTCEKDVLQG 
       460        470        480        490        500
ELEAVKQAKL KLEEKNRELE EELRKARAEA EDARQKAKDD DDSDIPTAQR 
       510        520        530        540        550
KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE NPAMQEKKRS 
       560        570        580        590        600
SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 
       610        620        630        640        650
GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL 
       660        670        680        690        700
PQKYKQVTNG QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT 
       710        720        730        740        750
RDGGSVVGAS VFYKDVAGLD TEGSKQRSAS QSSLDKLDQE LKEQQKELKN 
       760        770        780        790        800
QEELSSLVWI CTSTHSATKV LIIDAVQPGN ILDSFTVCNS HVLCIASVPG 
       810        820        830        840        850
ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG GITVVGCSAE 
       860        870        880        890        900
GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 
       910        920        930        940        950
SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV 
       960        970        980        990       1000
LPNEQDLVRE EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK 
      1010       1020       1030       1040       1050
DSILSIVHVK GIVLVALADG TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI 
      1060       1070       1080       1090       1100
RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK SFDAHPRKES QVRQLAWVGD 
      1110       1120       1130       1140       1150
GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK LGFSFVRITA 
      1160       1170       1180       1190       1200
LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK 
      1210       1220       1230       1240       1250
VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG 
      1260       1270       1280       1290       1300
DKAGPSAQEP GSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE 
      1310       1320 
PSVTKAERSH LIVWQVMYGN E
Length:1,321
Mass (Da):146,205
Last modified:May 2, 2006 - v4
Checksum:i5CAE349FBDF91B40
GO
Isoform 2 (identifier: O60271-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     555-555: F → FVPTR

Show »
Length:1,311
Mass (Da):145,135
Checksum:iD60A544D70BD1F97
GO
Isoform 3 (identifier: O60271-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Note: Due to intron retention.
Show »
Length:945
Mass (Da):104,736
Checksum:iEB17642AA2BF0BB4
GO
Isoform 4 (identifier: O60271-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.

Show »
Length:1,307
Mass (Da):144,682
Checksum:i55B30E422A8ABD0E
GO
Isoform 5 (identifier: O60271-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Show »
Length:931
Mass (Da):103,212
Checksum:i1A427D7B60367814
GO
Isoform 6 (identifier: O60271-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-196: MELEDGVVYQ...QLESTAHSRI → MSPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     1175-1175: T → TVILHQGRLLGLRA

Note: No experimental confirmation available.Curated
Show »
Length:1,177
Mass (Da):128,607
Checksum:i8CD074A547DCC690
GO

Sequence cautioni

The sequence AAH07524 differs from that shown. Probable cloning artifact.Curated
The sequence AAH59946 differs from that shown. Probable cloning artifact.Curated
The sequence AAI06049 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated
The sequence AAO66462 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA25442 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB14812 differs from that shown. Unlikely isoform. Aberrant splicing.Curated
The sequence CAA62987 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti451E → G in AAO66462 (PubMed:14662895).Curated1
Sequence conflicti680E → K in CAA62987 (PubMed:9480848).Curated1
Sequence conflicti680E → K in AAO66462 (PubMed:14662895).Curated1
Isoform 6 (identifier: O60271-9)
Sequence conflicti9F → S in BAG58134 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0593641320N → S. Corresponds to variant dbSNP:rs9896965Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0422531 – 196MELED…AHSRI → MSPGCMLLFVFGFVGGAVVI NSAILVSLSVLLLVHFSIST GVPALTQNLPRIL in isoform 6. 2 PublicationsAdd BLAST196
Alternative sequenceiVSP_018214248 – 261Missing in isoform 2, isoform 4, isoform 5 and isoform 6. 5 PublicationsAdd BLAST14
Alternative sequenceiVSP_018220555F → FVPTR in isoform 2. 1 Publication1
Alternative sequenceiVSP_018221938 – 945SNDSDAYK → RYNNGSST in isoform 3 and isoform 5. 2 Publications8
Alternative sequenceiVSP_018222946 – 1321Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST376
Alternative sequenceiVSP_0422541175T → TVILHQGRLLGLRA in isoform 6. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327452 mRNA. Translation: AAN61565.1.
AY850123 mRNA. Translation: AAX47276.1.
AB011088 mRNA. Translation: BAA25442.3. Different initiation.
AK024068 mRNA. Translation: BAB14812.1. Sequence problems.
AK295098 mRNA. Translation: BAG58134.1.
AK302789 mRNA. Translation: BAG63993.1.
AC005920 Genomic DNA. No translation available.
AC005839 Genomic DNA. No translation available.
BC007524 mRNA. Translation: AAH07524.1. Sequence problems.
BC059946 mRNA. Translation: AAH59946.1. Sequence problems.
BC106048 mRNA. Translation: AAI06049.1. Sequence problems.
BC146755 mRNA. Translation: AAI46756.1.
BC153878 mRNA. Translation: AAI53879.1.
X91879 mRNA. Translation: CAA62987.1. Different initiation.
AY219897 mRNA. Translation: AAO66462.1. Different initiation.
AY219898 mRNA. Translation: AAO66463.1.
CCDSiCCDS11577.1. [O60271-4]
CCDS45740.1. [O60271-1]
CCDS58577.1. [O60271-9]
CCDS58578.1. [O60271-2]
PIRiJC5958.
RefSeqiNP_001123999.1. NM_001130527.2. [O60271-2]
NP_001124000.1. NM_001130528.2. [O60271-1]
NP_001238900.1. NM_001251971.1. [O60271-9]
NP_003962.3. NM_003971.5. [O60271-4]
UniGeneiHs.463439.

Genome annotation databases

EnsembliENST00000262013; ENSP00000262013; ENSG00000008294. [O60271-1]
ENST00000357122; ENSP00000349636; ENSG00000008294. [O60271-4]
ENST00000505279; ENSP00000426900; ENSG00000008294. [O60271-2]
ENST00000510283; ENSP00000423165; ENSG00000008294. [O60271-9]
GeneIDi9043.
KEGGihsa:9043.
UCSCiuc002ita.4. human. [O60271-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiJIP4_HUMAN
AccessioniPrimary (citable) accession number: O60271
Secondary accession number(s): A6H8U5
, A8MSX0, B4DHH2, O60905, Q3KQU8, Q3MKM7, Q86WC7, Q86WC8, Q8IZX7, Q96II0, Q9H811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: June 7, 2017
This is version 148 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families