Reviewed, UniProtKB/Swiss-Prot O60271 (JIP4_HUMAN)

Names and origin

Protein names

Recommended name:
    C-jun-amino-terminal kinase-interacting protein 4
      Short name=JNK-interacting protein 4
      Short name=JIP-4
Alternative name(s):
    JNK-associated leucine-zipper protein
      Short name=JLP
    Sperm-associated antigen 9
    Mitogen-activated protein kinase 8-interacting protein 4
    Human lung cancer oncogene 6 protein
      Short name=HLC-6
    Proliferation-inducing protein 6
    Sperm-specific protein
    Sperm surface protein
    Protein highly expressed in testis
      Short name=PHET
    Sunday driver 1
    Cancer/testis antigen 89
      Short name=CT89

Gene names

Name:	SPAG9
Synonyms:	HSS, KIAA0516, MAPK8IP4, SYD1
ORF Names:	HLC6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction. Ref.12 Ref.13
Subunit structure	Homo-oligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Ref.12 Ref.13
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. Ref.13 Ref.9 Isoform 5: Acrosome. Note: Associated with the plasma membrane of the acrosomal compartment and also localizes in the acrosome matrix. Ref.13 Ref.9
Tissue specificity	Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Ref.9 Ref.2 Ref.8
Induction	Isoform 3 is increased in systemic sclerosis fibroblasts. Ref.9
Post-translational modification	Phosphorylated by MAPK8 and MAPK14 By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the JIP scaffold family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	spermatogenesis Ref.8 Traceable author statement. Source: ProtInc
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Ref.8 Traceable author statement. Source: ProtInc perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.12 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF4A2	Q14240	1	EBI-1023301,EBI-73473
NFKB1	P19838	1	EBI-1023301,EBI-300010

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60271-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60271-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     555-555: F → FVPTR

Isoform 3 (identifier: O60271-3)

The sequence of this isoform differs from the canonical sequence as follows:
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Isoform 4 (identifier: O60271-4)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.

Isoform 5 (identifier: O60271-5)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     938-945: SNDSDAYK → RYNNGSST
     946-1321: Missing.

Note: Associated with the plasma membrane of the acrosomal compartment and also localizes in the acrosome matrix. Ref.13 Ref.9

Isoform 6 (identifier: O60271-6)

The sequence of this isoform differs from the canonical sequence as follows:
     144-196: RLEEREAELK...QLESTAHSRI → MSPGCMLLFV...ALTQNLPRIL
     248-261: Missing.
     386-592: Missing.
     1072-1098: QPKAMKIEKSFDAHPRKESQVRQLAWV → HSSLGDGARPCLKTDKQAKNRLKSGFH
     1099-1321: Missing.

Note: No experimental confirmation available.

Isoform 7 (identifier: O60271-7)

The sequence of this isoform differs from the canonical sequence as follows:
     248-261: Missing.
     479-498: EAEDARQKAKDDDDSDIPTA → AEDDEDDDVDTKKQKTDEDD
     499-1321: Missing.

Note: No experimental confirmation available.

Isoform 8 (identifier: O60271-8)

The sequence of this isoform differs from the canonical sequence as follows:
     144-196: RLEEREAELK...QLESTAHSRI → MSPGCMLLFV...ALTQNLPRIL
     262-274: DELSDVSQGGSKA → VRMVLLNILQKVY
     275-1321: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1321	1321	C-jun-amino-terminal kinase-interacting protein 4		PRO_0000234076
Regions
	Coiled coil	66 – 166	101	Potential
	Coiled coil	408 – 534	127	Potential
	Coiled coil	724 – 758	35	Potential
Amino acid modifications
	Modified residue	109	1	Phosphoserine Ref.16
	Modified residue	183	1	Phosphoserine Ref.14 Ref.17 Ref.18
	Modified residue	185	1	Phosphoserine Ref.14 Ref.17 Ref.18
	Modified residue	203	1	Phosphoserine Ref.14 Ref.18
	Modified residue	217	1	Phosphothreonine Ref.14 Ref.18
	Modified residue	238	1	Phosphoserine Ref.18
	Modified residue	244	1	Phosphothreonine By similarity
	Modified residue	265	1	Phosphoserine Ref.16
	Modified residue	268	1	Phosphoserine Ref.16
	Modified residue	292	1	Phosphothreonine By similarity
	Modified residue	311	1	Phosphoserine Ref.18
	Modified residue	329	1	Phosphoserine Ref.18
	Modified residue	332	1	Phosphoserine Ref.18
	Modified residue	365	1	Phosphothreonine By similarity
	Modified residue	387	1	Phosphoserine Ref.14
	Modified residue	388	1	Phosphoserine Ref.14
	Modified residue	391	1	Phosphoserine Ref.14
	Modified residue	418	1	Phosphothreonine Ref.16 Ref.18
	Modified residue	586	1	Phosphothreonine Ref.18
	Modified residue	594	1	Phosphoserine Ref.15
	Modified residue	595	1	Phosphothreonine Ref.15
	Modified residue	730	1	Phosphoserine Ref.16 Ref.11
	Modified residue	732	1	Phosphoserine Ref.10
	Modified residue	733	1	Phosphoserine Ref.18 Ref.11 Ref.10
	Modified residue	1144	1	Phosphoserine Ref.18
Natural variations
	Alternative sequence	144 – 196	53	RLEER…AHSRI → MSPGCMLLFVFGFVGGAVVI NSAILVSLSVLLLVHFSIST GVPALTQNLPRIL in isoform 6 and isoform 8.		VSP_018213
	Alternative sequence	248 – 261	14	Missing in isoform 2, isoform 4, isoform 5, isoform 6 and isoform 7.		VSP_018214
	Alternative sequence	262 – 274	13	DELSD…GGSKA → VRMVLLNILQKVY in isoform 8.		VSP_018215
	Alternative sequence	275 – 1321	1047	Missing in isoform 8.		VSP_018216
	Alternative sequence	386 – 592	207	Missing in isoform 6.		VSP_018217
	Alternative sequence	479 – 498	20	EAEDA…DIPTA → AEDDEDDDVDTKKQKTDEDD in isoform 7.		VSP_018218
	Alternative sequence	499 – 1321	823	Missing in isoform 7.		VSP_018219
	Alternative sequence	555	1	F → FVPTR in isoform 2.		VSP_018220
	Alternative sequence	938 – 945	8	SNDSDAYK → RYNNGSST in isoform 3 and isoform 5.		VSP_018221
	Alternative sequence	946 – 1321	376	Missing in isoform 3 and isoform 5.		VSP_018222
	Alternative sequence	1072 – 1098	27	QPKAM…QLAWV → HSSLGDGARPCLKTDKQAKN RLKSGFH in isoform 6.		VSP_018223
	Alternative sequence	1099 – 1321	223	Missing in isoform 6.		VSP_018224
Experimental info
	Sequence conflict	451	1	E → G in AAO66462. Ref.9
	Sequence conflict	680	1	E → K in CAA62987. Ref.8
	Sequence conflict	680	1	E → K in AAO66462. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 4. Checksum: 5CAE349FBDF91B40	FASTA	1,321	146,205
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKVSN SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 310 320 330 340 350 360 FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE LDMDKDLSGY 370 380 390 400 410 420 KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 430 440 450 460 470 480 LETKNALNIV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEEKNRELE EELRKARAEA 490 500 510 520 530 540 EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 550 560 570 580 590 600 NPAMQEKKRS SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 610 620 630 640 650 660 GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG 670 680 690 700 710 720 QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD 730 740 750 760 770 780 TEGSKQRSAS QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN 790 800 810 820 830 840 ILDSFTVCNS HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG 850 860 870 880 890 900 GITVVGCSAE GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 910 920 930 940 950 960 SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV LPNEQDLVRE 970 980 990 1000 1010 1020 EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG 1030 1040 1050 1060 1070 1080 TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK 1090 1100 1110 1120 1130 1140 SFDAHPRKES QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK 1150 1160 1170 1180 1190 1200 LGFSFVRITA LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK 1210 1220 1230 1240 1250 1260 VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG DKAGPSAQEP 1270 1280 1290 1300 1310 1320 GSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMYGN E
	Isoform 2. Checksum: D60A544D70BD1F97	FASTA	1,311	145,135
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FVPTRFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP GDKSKAFDFL 610 620 630 640 650 660 SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG QGENKMKNLP 670 680 690 700 710 720 VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD TEGSKQRSAS 730 740 750 760 770 780 QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN ILDSFTVCNS 790 800 810 820 830 840 HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG GITVVGCSAE 850 860 870 880 890 900 GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG SAEDTVDISQ 910 920 930 940 950 960 TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV LPNEQDLVRE EAQKMSSLLP 970 980 990 1000 1010 1020 TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG TLAIFHRGVD 1030 1040 1050 1060 1070 1080 GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK SFDAHPRKES 1090 1100 1110 1120 1130 1140 QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK LGFSFVRITA 1150 1160 1170 1180 1190 1200 LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK VTPGTFIPYC 1210 1220 1230 1240 1250 1260 SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG DKAGPSAQEP GSQTPLKSML 1270 1280 1290 1300 1310 VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMYGN E
	Isoform 3. Checksum: EB17642AA2BF0BB4	FASTA	945	104,736
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKVSN SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 310 320 330 340 350 360 FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE LDMDKDLSGY 370 380 390 400 410 420 KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 430 440 450 460 470 480 LETKNALNIV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEEKNRELE EELRKARAEA 490 500 510 520 530 540 EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 550 560 570 580 590 600 NPAMQEKKRS SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 610 620 630 640 650 660 GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG 670 680 690 700 710 720 QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD 730 740 750 760 770 780 TEGSKQRSAS QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN 790 800 810 820 830 840 ILDSFTVCNS HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG 850 860 870 880 890 900 GITVVGCSAE GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 910 920 930 940 SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSRYN NGSST
	Isoform 4. Checksum: 55B30E422A8ABD0E	FASTA	1,307	144,682
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NTTKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVTNGQGEN KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDTS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DVAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKELKNQEEL SSLVWICTST HSATKVLIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEDLSE SGQVDKASLC GSMTSNSSAE TDSLLGGITV VGCSAEGVTG 850 860 870 880 890 900 AATSPSTNGA SPVMDKPPEM EAENSEVDEN VPTAEEATEA TEGNAGSAED TVDISQTGVY 910 920 930 940 950 960 TEHVFTDPLG VQIPEDLSPV YQSSNDSDAY KDQISVLPNE QDLVREEAQK MSSLLPTMWL 970 980 990 1000 1010 1020 GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD 1030 1040 1050 1060 1070 1080 LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ 1090 1100 1110 1120 1130 1140 LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS 1150 1160 1170 1180 1190 1200 CNRLWVGTGN GVIISIPLTE TNKTSGVPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH 1210 1220 1230 1240 1250 1260 AQLCFHGHRD AVKFFVAVPG QVISPQSSSS GTDLTGDKAG PSAQEPGSQT PLKSMLVISG 1270 1280 1290 1300 GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMYGNE
	Isoform 5. Checksum: 1A427D7B60367814	FASTA	931	103,212
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD 490 500 510 520 530 540 IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ 550 560 570 580 590 600 FFSRLFSSSS NTTKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET 610 620 630 640 650 660 EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVTNGQGEN KMKNLPVPVY 670 680 690 700 710 720 LRPLDEKDTS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DVAGLDTEGS KQRSASQSSL 730 740 750 760 770 780 DKLDQELKEQ QKELKNQEEL SSLVWICTST HSATKVLIID AVQPGNILDS FTVCNSHVLC 790 800 810 820 830 840 IASVPGARET DYPAGEDLSE SGQVDKASLC GSMTSNSSAE TDSLLGGITV VGCSAEGVTG 850 860 870 880 890 900 AATSPSTNGA SPVMDKPPEM EAENSEVDEN VPTAEEATEA TEGNAGSAED TVDISQTGVY 910 920 930 TEHVFTDPLG VQIPEDLSPV YQSRYNNGSS T
	Isoform 6. Checksum: B92A61E7E996D0D4	FASTA	877	95,835
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISMSPGCML LFVFGFVGGA VVINSAILVS LSVLLLVHFS 190 200 210 220 230 240 ISTGVPALTQ NLPRILRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ESSTLSQLPG DKSKAFDFLS EETEASLASR REQKREQYRQ VKAHVQKEDG 430 440 450 460 470 480 RVQAFGWSLP QKYKQVTNGQ GENKMKNLPV PVYLRPLDEK DTSMKLWCAV GVNLSGGKTR 490 500 510 520 530 540 DGGSVVGASV FYKDVAGLDT EGSKQRSASQ SSLDKLDQEL KEQQKELKNQ EELSSLVWIC 550 560 570 580 590 600 TSTHSATKVL IIDAVQPGNI LDSFTVCNSH VLCIASVPGA RETDYPAGED LSESGQVDKA 610 620 630 640 650 660 SLCGSMTSNS SAETDSLLGG ITVVGCSAEG VTGAATSPST NGASPVMDKP PEMEAENSEV 670 680 690 700 710 720 DENVPTAEEA TEATEGNAGS AEDTVDISQT GVYTEHVFTD PLGVQIPEDL SPVYQSSNDS 730 740 750 760 770 780 DAYKDQISVL PNEQDLVREE AQKMSSLLPT MWLGAQNGCL YVHSSVAQWR KCLHSIKLKD 790 800 810 820 830 840 SILSIVHVKG IVLVALADGT LAIFHRGVDG QWDLSNYHLL DLGRPHHSIR CMTVVHDKVW 850 860 870 CGYRNKIYVV HSSLGDGARP CLKTDKQAKN RLKSGFH
	Isoform 7. Checksum: 9A52D729C0CA2173	FASTA	484	54,396
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 190 200 210 220 230 240 QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 280 290 300 RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS 310 320 330 340 350 360 KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA 370 380 390 400 410 420 FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL 430 440 450 460 470 480 IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAAEDDED DDVDTKKQKT DEDD
	Isoform 8. Checksum: 25744A0EBDD2EA22	FASTA	274	30,769
	10 20 30 40 50 60 MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 70 80 90 100 110 120 LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 130 140 150 160 170 180 VESLESQTRQ LELKAKNYAD QISMSPGCML LFVFGFVGGA VVINSAILVS LSVLLLVHFS 190 200 210 220 230 240 ISTGVPALTQ NLPRILRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 250 260 270 RSHTSLKVSN SPEPQKAVEQ EVRMVLLNIL QKVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JLP: a scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors." Lee C.M., Onesime D., Reddy C.D., Dhanasekaran N., Reddy E.P. Proc. Natl. Acad. Sci. U.S.A. 99:14189-14194(2002) [PubMed: 12391307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Liver.
[2]	"Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia." Guinn B.-A., Bland E.A., Lodi U., Liggins A.P., Tobal K., Petters S., Wells J.W., Banham A.H., Mufti G.J. Biochem. Biophys. Res. Commun. 335:1293-1304(2005) [PubMed: 16112646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY.
[3]	"Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:31-39(1998) [PubMed: 9628581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6).
[6]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 7 AND 8). Tissue: Eye and Kidney.
[8]	"Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif." Shankar S., Mohapatra B., Suri A. Biochem. Biophys. Res. Commun. 243:561-565(1998) [PubMed: 9480848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 130-945 (ISOFORM 5), TISSUE SPECIFICITY. Tissue: Testis.
[9]	"A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies." Yasuoka H., Ihn H., Medsger T.A. Jr., Hirakata M., Kawakami Y., Ikeda Y., Kuwana M. J. Immunol. 171:6883-6890(2003) [PubMed: 14662895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 162-945 (ISOFORM 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION. Tissue: Testis.
[10]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-732 AND SER-733, MASS SPECTROMETRY. Tissue: T-cell.
[11]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-730 AND SER-733, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway." Bouwmeester T., Bauch A., Ruffner H., Angrand P.-O., Bergamini G., Croughton K., Cruciat C., Eberhard D., Gagneur J., Ghidelli S., Hopf C., Huhse B., Mangano R., Michon A.-M., Schirle M., Schlegl J., Schwab M., Stein M.A. , Bauer A., Casari G., Drewes G., Gavin A.-C., Jackson D.B., Joberty G., Neubauer G., Rick J., Kuster B., Superti-Furga G. Nat. Cell Biol. 6:97-105(2004) [PubMed: 14743216] [Abstract] Cited for: FUNCTION, INTERACTION WITH NFKB1.
[13]	"Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein." Jagadish N., Rana R., Selvi R., Mishra D., Garg M., Yadav S., Herr J.C., Okumura K., Hasegawa A., Koyama K., Suri A. Biochem. J. 389:73-82(2005) [PubMed: 15693750] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAPK9; MAPK10 AND MAPK8, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[14]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-185; SER-203; THR-217; SER-387; SER-388 AND SER-391, MASS SPECTROMETRY. Tissue: Epithelium.
[15]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-594 AND THR-595, MASS SPECTROMETRY.
[16]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109; SER-265; SER-268; THR-418 AND SER-730, MASS SPECTROMETRY.
[17]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183 AND SER-185, MASS SPECTROMETRY.
[18]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-185; SER-203; THR-217; SER-238; SER-311; SER-329; SER-332; THR-418; THR-586; SER-733 AND SER-1144, MASS SPECTROMETRY.
[19]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF327452 mRNA. Translation: AAN61565.1. AY850123 mRNA. Translation: AAX47276.1. AB011088 mRNA. Translation: BAA25442.3. Different initiation. AK024068 mRNA. Translation: BAB14812.1. AC005920 Genomic DNA. No translation available. AC005839 Genomic DNA. No translation available. BC007524 mRNA. Translation: AAH07524.1. BC059946 mRNA. Translation: AAH59946.1. BC106048 mRNA. Translation: AAI06049.1. X91879 mRNA. Translation: CAA62987.1. Different initiation. AY219897 mRNA. Translation: AAO66462.1. Different initiation. AY219898 mRNA. Translation: AAO66463.1.
IPI	IPI00303397. IPI00384432. IPI00479676. IPI00744288. IPI00744648. IPI00748244. IPI00748833. IPI00879552.
PIR	JC5958.
RefSeq	NP_001124000.1. NP_003962.3.
UniGene	Hs.463439
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O60271. 2 interactions.
PTM databases
PhosphoSite	O60271.
Proteomic databases
PRIDE	O60271.
Genome annotation databases
Ensembl	ENSG00000008294. Homo sapiens. [Contig view]
GeneID	9043.
KEGG	hsa:9043.
Organism-specific databases
GeneCards	GC17M046397.
H-InvDB	HIX0013991.
HGNC	HGNC:14524. SPAG9.
MIM	605430. gene.
PharmGKB	PA37890.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O60271.
Gene expression databases
ArrayExpress	O60271.
Bgee	O60271.
GermOnline	ENSG00000008294. Homo sapiens.
Family and domain databases
InterPro	IPR019143. JNK/Rab-associated_protein-1. [Graphical view]
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33871.
PMAP-CutDB	O60271.
SOURCE	Search...

Entry information

Entry name

JIP4_HUMAN

Accession

Primary (citable) accession number: O60271
Secondary accession number(s): A8MSX0 , O60905, Q3KQU8, Q3MKM7, Q86WC7, Q86WC8, Q8IZX7, Q96II0, Q9H811

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 63 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)