UniProtKB - O60264 (SMCA5_HUMAN)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
Gene
SMARCA5
Organism
Homo sapiens (Human)
Status
Functioni
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.7 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 205 – 212 | ATPCurated | 8 |
GO - Molecular functioni
- ATPase activity Source: UniProtKB
- ATP binding Source: UniProtKB
- DNA binding Source: UniProtKB
- helicase activity Source: ProtInc
- nucleosome binding Source: InterPro
GO - Biological processi
- ATP-dependent chromatin remodeling Source: BHF-UCL
- cellular response to leukemia inhibitory factor Source: Ensembl
- CENP-A containing nucleosome assembly Source: Reactome
- chromatin remodeling Source: UniProtKB
- chromatin silencing at rDNA Source: Ensembl
- covalent chromatin modification Source: UniProtKB-KW
- DNA-templated transcription, initiation Source: UniProtKB
- nucleosome assembly Source: UniProtKB
- nucleosome positioning Source: UniProtKB
- positive regulation of gene expression, epigenetic Source: Reactome
- positive regulation of transcription, DNA-templated Source: Ensembl
- regulation of transcription by RNA polymerase II Source: ProtInc
Keywordsi
Molecular function | Chromatin regulator, Helicase, Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-HSA-427413. NoRC negatively regulates rRNA expression. R-HSA-5250924. B-WICH complex positively regulates rRNA expression. R-HSA-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks. R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere. |
Names & Taxonomyi
Protein namesi | Recommended name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (EC:3.6.4.-)Short name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 Alternative name(s): Sucrose nonfermenting protein 2 homolog Short name: hSNF2H |
Gene namesi | Name:SMARCA5 Synonyms:SNF2H, WCRF135 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000153147.5. |
HGNCi | HGNC:11101. SMARCA5. |
MIMi | 603375. gene. |
neXtProti | NX_O60264. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 211 | K → R: Loss of ATP hydrolysis and no association of the SMARCA5/cohesin/NuRD complex with chromatin. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 8467. |
MalaCardsi | SMARCA5. |
OpenTargetsi | ENSG00000153147. |
Orphaneti | 370334. Extraskeletal Ewing sarcoma. |
PharmGKBi | PA35951. |
Chemistry databases
DrugBanki | DB02670. 4-Deoxy-Alpha-D-Glucose. DB02379. Beta-D-Glucose. |
Polymorphism and mutation databases
BioMutai | SMARCA5. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000074354 | 2 – 1052 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5Add BLAST | 1051 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 66 | PhosphoserineCombined sources | 1 | |
Cross-linki | 83 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 113 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 116 | PhosphoserineCombined sources | 1 | |
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 171 | PhosphoserineCombined sources | 1 | |
Modified residuei | 440 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 644 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 647 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 694 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 722 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 735 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 755 | PhosphoserineCombined sources | 1 | |
Modified residuei | 825 | PhosphoserineCombined sources | 1 | |
Cross-linki | 966 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O60264. |
MaxQBi | O60264. |
PaxDbi | O60264. |
PeptideAtlasi | O60264. |
PRIDEi | O60264. |
PTM databases
iPTMneti | O60264. |
PhosphoSitePlusi | O60264. |
SwissPalmi | O60264. |
Expressioni
Tissue specificityi
Ubiquitously expressed.
Developmental stagei
Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.1 Publication
Gene expression databases
Bgeei | ENSG00000153147. |
CleanExi | HS_SMARCA5. |
Genevisiblei | O60264. HS. |
Organism-specific databases
HPAi | CAB005227. HPA008751. |
Interactioni
Subunit structurei
Catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. ACF/WCRF contains BAZ1A; CHRAC contains BAZ1A, CHRAC1, and POLE3; RSF contains RSF1; WICH contains BAZ1B/WSTF. SMARCA5 is the catalytic subunit of the NoRC chromatin-remodeling complex, which also contains BAZ2A/TIP5. The BAZ2A/TIP5 subunit of NoRC also interacts with DNMT1, DNMT3B and HDAC1, which allows NoRC to actively suppress rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation. Catalytic subunit of SMARCA5/cohesin/NuRD complexes. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Interacts with MYO1C (By similarity). Interacts with BEND3.By similarity1 Publication
Binary interactionsi
Protein-protein interaction databases
BioGridi | 114045. 137 interactors. |
CORUMi | O60264. |
DIPi | DIP-33204N. |
IntActi | O60264. 55 interactors. |
MINTi | O60264. |
STRINGi | 9606.ENSP00000283131. |
Structurei
3D structure databases
ProteinModelPortali | O60264. |
SMRi | O60264. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 192 – 357 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 487 – 638 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 152 | |
Domaini | 840 – 892 | SANT 1PROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 943 – 1007 | SANT 2PROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 308 – 311 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 13 | Poly-Pro | 7 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0385. Eukaryota. COG0553. LUCA. |
GeneTreei | ENSGT00900000140963. |
HOGENOMi | HOG000192862. |
HOVERGENi | HBG056329. |
InParanoidi | O60264. |
KOi | K11654. |
OMAi | MAFTEWI. |
OrthoDBi | EOG091G01GJ. |
PhylomeDBi | O60264. |
TreeFami | TF300674. |
Family and domain databases
CDDi | cd00079. HELICc. 1 hit. cd00167. SANT. 2 hits. |
Gene3Di | 1.10.1040.30. 1 hit. |
InterProi | View protein in InterPro IPR020838. DBINO. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR009057. Homeobox-like_sf. IPR029915. ISWI. IPR015194. ISWI_HAND-dom. IPR036306. ISWI_HAND-dom_sf. IPR027417. P-loop_NTPase. IPR001005. SANT/Myb. IPR017884. SANT_dom. IPR015195. SLIDE. IPR000330. SNF2_N. |
PANTHERi | PTHR10799:SF887. PTHR10799:SF887. 1 hit. |
Pfami | View protein in Pfam PF13892. DBINO. 1 hit. PF09110. HAND. 1 hit. PF00271. Helicase_C. 1 hit. PF09111. SLIDE. 1 hit. PF00176. SNF2_N. 1 hit. |
SMARTi | View protein in SMART SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00717. SANT. 2 hits. |
SUPFAMi | SSF101224. SSF101224. 1 hit. SSF46689. SSF46689. 2 hits. SSF52540. SSF52540. 2 hits. |
PROSITEi | View protein in PROSITE PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51293. SANT. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O60264-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAAEPPPP PPPESAPSKP AASIASGGSN SSNKGGPEGV AAQAVASAAS
60 70 80 90 100
AGPADAEMEE IFDDASPGKQ KEIQEPDPTY EEKMQTDRAN RFEYLLKQTE
110 120 130 140 150
LFAHFIQPAA QKTPTSPLKM KPGRPRIKKD EKQNLLSVGD YRHRRTEQEE
160 170 180 190 200
DEELLTESSK ATNVCTRFED SPSYVKWGKL RDYQVRGLNW LISLYENGIN
210 220 230 240 250
GILADEMGLG KTLQTISLLG YMKHYRNIPG PHMVLVPKST LHNWMSEFKR
260 270 280 290 300
WVPTLRSVCL IGDKEQRAAF VRDVLLPGEW DVCVTSYEML IKEKSVFKKF
310 320 330 340 350
NWRYLVIDEA HRIKNEKSKL SEIVREFKTT NRLLLTGTPL QNNLHELWSL
360 370 380 390 400
LNFLLPDVFN SADDFDSWFD TNNCLGDQKL VERLHMVLRP FLLRRIKADV
410 420 430 440 450
EKSLPPKKEV KIYVGLSKMQ REWYTRILMK DIDILNSAGK MDKMRLLNIL
460 470 480 490 500
MQLRKCCNHP YLFDGAEPGP PYTTDMHLVT NSGKMVVLDK LLPKLKEQGS
510 520 530 540 550
RVLIFSQMTR VLDILEDYCM WRNYEYCRLD GQTPHDERQD SINAYNEPNS
560 570 580 590 600
TKFVFMLSTR AGGLGINLAT ADVVILYDSD WNPQVDLQAM DRAHRIGQTK
610 620 630 640 650
TVRVFRFITD NTVEERIVER AEMKLRLDSI VIQQGRLVDQ NLNKIGKDEM
660 670 680 690 700
LQMIRHGATH VFASKESEIT DEDIDGILER GAKKTAEMNE KLSKMGESSL
710 720 730 740 750
RNFTMDTESS VYNFEGEDYR EKQKIAFTEW IEPPKRERKA NYAVDAYFRE
760 770 780 790 800
ALRVSEPKAP KAPRPPKQPN VQDFQFFPPR LFELLEKEIL FYRKTIGYKV
810 820 830 840 850
PRNPELPNAA QAQKEEQLKI DEAESLNDEE LEEKEKLLTQ GFTNWNKRDF
860 870 880 890 900
NQFIKANEKW GRDDIENIAR EVEGKTPEEV IEYSAVFWER CNELQDIEKI
910 920 930 940 950
MAQIERGEAR IQRRISIKKA LDTKIGRYKA PFHQLRISYG TNKGKNYTEE
960 970 980 990 1000
EDRFLICMLH KLGFDKENVY DELRQCIRNS PQFRFDWFLK SRTAMELQRR
1010 1020 1030 1040 1050
CNTLITLIER ENMELEEKEK AEKKKRGPKP STQKRKMDGA PDGRGRKKKL
KL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB010882 mRNA. Translation: BAA25173.1. BC023144 mRNA. Translation: AAH23144.1. |
CCDSi | CCDS3761.1. |
RefSeqi | NP_003592.3. NM_003601.3. |
UniGenei | Hs.558422. |
Genome annotation databases
Ensembli | ENST00000283131; ENSP00000283131; ENSG00000153147. |
GeneIDi | 8467. |
KEGGi | hsa:8467. |
UCSCi | uc003ijg.4. human. |
Similar proteinsi
Entry informationi
Entry namei | SMCA5_HUMAN | |
Accessioni | O60264Primary (citable) accession number: O60264 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2004 |
Last sequence update: | August 1, 1998 | |
Last modified: | March 28, 2018 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |