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Protein

Mediator of RNA polymerase II transcription subunit 14

Gene

MED14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.3 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • receptor activity Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • transcription coactivator activity Source: MGI
  • transcription cofactor activity Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  • androgen receptor signaling pathway Source: UniProtKB
  • intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • stem cell population maintenance Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180182-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SignaLinkiO60244.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 14
Alternative name(s):
Activator-recruited cofactor 150 kDa component
Short name:
ARC150
Cofactor required for Sp1 transcriptional activation subunit 2
Short name:
CRSP complex subunit 2
Mediator complex subunit 14
RGR1 homolog
Short name:
hRGR1
Thyroid hormone receptor-associated protein complex 170 kDa component
Short name:
Trap170
Transcriptional coactivator CRSP150
Vitamin D3 receptor-interacting protein complex 150 kDa component
Short name:
DRIP150
Gene namesi
Name:MED14
Synonyms:ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:2370. MED14.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • mediator complex Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9282.
OpenTargetsiENSG00000180182.
PharmGKBiPA26890.

Polymorphism and mutation databases

BioMutaiMED14.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793571 – 1454Mediator of RNA polymerase II transcription subunit 14Add BLAST1454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei617PhosphoserineCombined sources1
Modified residuei986PhosphoserineCombined sources1
Modified residuei1112PhosphoserineCombined sources1
Modified residuei1119PhosphoserineCombined sources1
Modified residuei1128PhosphoserineCombined sources1
Modified residuei1136PhosphoserineCombined sources1
Modified residuei1144PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60244.
MaxQBiO60244.
PaxDbiO60244.
PeptideAtlasiO60244.
PRIDEiO60244.

PTM databases

iPTMnetiO60244.
PhosphoSitePlusiO60244.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000180182.
CleanExiHS_MED14.
ExpressionAtlasiO60244. baseline and differential.
GenevisibleiO60244. HS.

Organism-specific databases

HPAiHPA064182.

Interactioni

Subunit structurei

Interacts with GATA1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR, ESR1, SREBF1 and STAT2.By similarity12 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED15Q96RN52EBI-394489,EBI-394506
MED16Q9Y2X02EBI-394489,EBI-394541
MED23Q9ULK42EBI-394489,EBI-311161

GO - Molecular functioni

  • vitamin D receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114699. 74 interactors.
DIPiDIP-31460N.
IntActiO60244. 36 interactors.
MINTiMINT-2796527.
STRINGi9606.ENSP00000323720.

Structurei

3D structure databases

ProteinModelPortaliO60244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 566Interaction with STAT21 PublicationAdd BLAST379
Regioni500 – 824Interaction with SREBF11 PublicationAdd BLAST325

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 73LXXLL motif 15
Motifi1182 – 1186LXXLL motif 25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1002 – 1165Pro-richAdd BLAST164

Sequence similaritiesi

Belongs to the Mediator complex subunit 14 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1875. Eukaryota.
ENOG410XWK4. LUCA.
GeneTreeiENSGT00390000001021.
HOGENOMiHOG000045361.
HOVERGENiHBG104308.
InParanoidiO60244.
KOiK15156.
OMAiPKRSGEM.
OrthoDBiEOG091G01FK.
PhylomeDBiO60244.
TreeFamiTF314388.

Family and domain databases

InterProiIPR013947. Mediator_Med14.
[Graphical view]
PfamiPF08638. Med14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR
60 70 80 90 100
LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFAS RTRQLFVRLL
110 120 130 140 150
ALVKWANNAG KVEKCAMISS FLDQQAILFV DTADRLASLA RDALVHARLP
160 170 180 190 200
SFAIPYAIDV LTTGSYPRLP TCIRDKIIPP DPITKIEKQA TLHQLNQILR
210 220 230 240 250
HRLVTTDLPP QLANLTVANG RVKFRVEGEF EATLTVMGDD PDVPWRLLKL
260 270 280 290 300
EILVEDKETG DGRALVHSMQ ISFIHQLVQS RLFADEKPLQ DMYNCLHSFC
310 320 330 340 350
LSLQLEVLHS QTLMLIRERW GDLVQVERYH AGKCLSLSVW NQQVLGRKTG
360 370 380 390 400
TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDHLSIEKL
410 420 430 440 450
LIDSVHARAH QKLQELKAIL RGFNANENSS IETALPALVV PILEPCGNSE
460 470 480 490 500
CLHIFVDLHS GMFQLMLYGL DQATLDDMEK SVNDDMKRII PWIQQLKFWL
510 520 530 540 550
GQQRCKQSIK HLPTISSETL QLSNYSTHPI GNLSKNKLFI KLTRLPQYYI
560 570 580 590 600
VVEMLEVPNK PTQLSYKYYF MSVNAADRED SPAMALLLQQ FKENIQDLVF
610 620 630 640 650
RTKTGKQTRT NAKRKLSDDP CPVESKKTKR AGEMCAFNKV LAHFVAMCDT
660 670 680 690 700
NMPFVGLRLE LSNLEIPHQG VQVEGDGFSH AIRLLKIPPC KGITEETQKA
710 720 730 740 750
LDRSLLDCTF RLQGRNNRTW VAELVFANCP LNGTSTREQG PSRHVYLTYE
760 770 780 790 800
NLLSEPVGGR KVVEMFLNDW NSIARLYECV LEFARSLPDI PAHLNIFSEV
810 820 830 840 850
RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT
860 870 880 890 900
ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVPM LGLTQRTNTA
910 920 930 940 950
YQCFSILPQS STHIRLAFRN MYCIDIYCRS RGVVAIRDGA YSLFDNSKLV
960 970 980 990 1000
EGFYPAPGLK TFLNMFVDSN QDARRRSVNE DDNPPSPIGG DMMDSLISQL
1010 1020 1030 1040 1050
QPPPQQQPFP KQPGTSGAYP LTSPPTSYHS TVNQSPSMMH TQSPGNLHAA
1060 1070 1080 1090 1100
SSPSGALRAP SPASFVPTPP PSSHGISIGP GASFASPHGT LDPSSPYTMV
1110 1120 1130 1140 1150
SPSGRAGNWP GSPQVSGPSP AARMPGMSPA NPSLHSPVPD ASHSPRAGTS
1160 1170 1180 1190 1200
SQTMPTNMPP PRKLPQRSWA ASIPTILTHS ALNILLLPSP TPGLVPGLAG
1210 1220 1230 1240 1250
SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR
1260 1270 1280 1290 1300
VALSPKTNQT LQLKVTPENA GQWKPDELQV LEKFFETRVA GPPFKANTLI
1310 1320 1330 1340 1350
AFTKLLGAPT HILRDCVHIM KLELFPDQAT QLKWNVQFCL TIPPSAPPIA
1360 1370 1380 1390 1400
PPGTPAVVLK SKMLFFLQLT QKTSVPPQEP VSIIVPIIYD MASGTTQQAD
1410 1420 1430 1440 1450
IPRQQNSSVA APMMVSNILK RFAEMNPPRQ GECTIFAAVR DLMANLTLPP

GGRP
Length:1,454
Mass (Da):160,607
Last modified:April 17, 2007 - v2
Checksum:i144308E3F08D3D9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309 – 310HS → LT in AAD24360 (PubMed:10024883).Curated2
Sequence conflicti1265V → L in BAA28626 (PubMed:9598311).Curated1
Sequence conflicti1265V → L in AAD12725 (PubMed:9989412).Curated1
Sequence conflicti1265V → L in AAG22547 (PubMed:10235266).Curated1
Sequence conflicti1451G → V in BAA28626 (PubMed:9598311).Curated1
Sequence conflicti1451G → V in AAD12725 (PubMed:9989412).Curated1
Sequence conflicti1451G → V in AAG22547 (PubMed:10235266).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0366081325F → L in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006651 mRNA. Translation: BAA28626.1.
AB006652 Genomic DNA. Translation: BAA28627.1.
AF104256 mRNA. Translation: AAD12725.1.
AF304448 mRNA. Translation: AAG22547.1.
AF135802 mRNA. Translation: AAD24360.1.
BC098377 mRNA. Translation: AAH98377.1.
BC132672 mRNA. Translation: AAI32673.1.
BC132674 mRNA. Translation: AAI32675.1.
CCDSiCCDS14254.1.
RefSeqiNP_004220.2. NM_004229.3.
UniGeneiHs.407604.

Genome annotation databases

EnsembliENST00000324817; ENSP00000323720; ENSG00000180182.
GeneIDi9282.
KEGGihsa:9282.
UCSCiuc004dex.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006651 mRNA. Translation: BAA28626.1.
AB006652 Genomic DNA. Translation: BAA28627.1.
AF104256 mRNA. Translation: AAD12725.1.
AF304448 mRNA. Translation: AAG22547.1.
AF135802 mRNA. Translation: AAD24360.1.
BC098377 mRNA. Translation: AAH98377.1.
BC132672 mRNA. Translation: AAI32673.1.
BC132674 mRNA. Translation: AAI32675.1.
CCDSiCCDS14254.1.
RefSeqiNP_004220.2. NM_004229.3.
UniGeneiHs.407604.

3D structure databases

ProteinModelPortaliO60244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114699. 74 interactors.
DIPiDIP-31460N.
IntActiO60244. 36 interactors.
MINTiMINT-2796527.
STRINGi9606.ENSP00000323720.

PTM databases

iPTMnetiO60244.
PhosphoSitePlusiO60244.

Polymorphism and mutation databases

BioMutaiMED14.

Proteomic databases

EPDiO60244.
MaxQBiO60244.
PaxDbiO60244.
PeptideAtlasiO60244.
PRIDEiO60244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324817; ENSP00000323720; ENSG00000180182.
GeneIDi9282.
KEGGihsa:9282.
UCSCiuc004dex.5. human.

Organism-specific databases

CTDi9282.
DisGeNETi9282.
GeneCardsiMED14.
HGNCiHGNC:2370. MED14.
HPAiHPA064182.
MIMi300182. gene.
neXtProtiNX_O60244.
OpenTargetsiENSG00000180182.
PharmGKBiPA26890.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1875. Eukaryota.
ENOG410XWK4. LUCA.
GeneTreeiENSGT00390000001021.
HOGENOMiHOG000045361.
HOVERGENiHBG104308.
InParanoidiO60244.
KOiK15156.
OMAiPKRSGEM.
OrthoDBiEOG091G01FK.
PhylomeDBiO60244.
TreeFamiTF314388.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180182-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SignaLinkiO60244.

Miscellaneous databases

ChiTaRSiMED14. human.
GeneWikiiMED14.
GenomeRNAii9282.
PROiO60244.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180182.
CleanExiHS_MED14.
ExpressionAtlasiO60244. baseline and differential.
GenevisibleiO60244. HS.

Family and domain databases

InterProiIPR013947. Mediator_Med14.
[Graphical view]
PfamiPF08638. Med14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED14_HUMAN
AccessioniPrimary (citable) accession number: O60244
Secondary accession number(s): Q4KMR7, Q9UNB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: April 17, 2007
Last modified: November 2, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.