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Protein

Mediator of RNA polymerase II transcription subunit 14

Gene

MED14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.3 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • signaling receptor activity Source: UniProtKB
  • transcription coactivator activity Source: MGI
  • transcription cofactor activity Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  • androgen receptor signaling pathway Source: UniProtKB
  • intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of transcription by RNA polymerase II Source: ProtInc
  • stem cell population maintenance Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
SignaLinkiO60244

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 14
Alternative name(s):
Activator-recruited cofactor 150 kDa component
Short name:
ARC150
Cofactor required for Sp1 transcriptional activation subunit 2
Short name:
CRSP complex subunit 2
Mediator complex subunit 14
RGR1 homolog
Short name:
hRGR1
Thyroid hormone receptor-associated protein complex 170 kDa component
Short name:
Trap170
Transcriptional coactivator CRSP150
Vitamin D3 receptor-interacting protein complex 150 kDa component
Short name:
DRIP150
Gene namesi
Name:MED14
Synonyms:ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000180182.10
HGNCiHGNC:2370 MED14
MIMi300182 gene
neXtProtiNX_O60244

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9282
OpenTargetsiENSG00000180182
PharmGKBiPA26890

Polymorphism and mutation databases

BioMutaiMED14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793571 – 1454Mediator of RNA polymerase II transcription subunit 14Add BLAST1454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei617PhosphoserineCombined sources1
Modified residuei986PhosphoserineCombined sources1
Modified residuei1112PhosphoserineCombined sources1
Modified residuei1119PhosphoserineCombined sources1
Modified residuei1128PhosphoserineCombined sources1
Modified residuei1136PhosphoserineCombined sources1
Modified residuei1144PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60244
MaxQBiO60244
PaxDbiO60244
PeptideAtlasiO60244
PRIDEiO60244

PTM databases

iPTMnetiO60244
PhosphoSitePlusiO60244

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000180182
CleanExiHS_MED14
ExpressionAtlasiO60244 baseline and differential
GenevisibleiO60244 HS

Organism-specific databases

HPAiHPA064182

Interactioni

Subunit structurei

Interacts with GATA1 (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR, ESR1, SREBF1 and STAT2.By similarity12 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • vitamin D receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114699, 76 interactors
CORUMiO60244
DIPiDIP-31460N
IntActiO60244, 40 interactors
MINTiO60244
STRINGi9606.ENSP00000323720

Structurei

3D structure databases

ProteinModelPortaliO60244
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 566Interaction with STAT21 PublicationAdd BLAST379
Regioni500 – 824Interaction with SREBF11 PublicationAdd BLAST325

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 73LXXLL motif 15
Motifi1182 – 1186LXXLL motif 25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1002 – 1165Pro-richAdd BLAST164

Sequence similaritiesi

Belongs to the Mediator complex subunit 14 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1875 Eukaryota
ENOG410XWK4 LUCA
GeneTreeiENSGT00390000001021
HOGENOMiHOG000045361
HOVERGENiHBG104308
InParanoidiO60244
KOiK15156
OMAiCHNTILH
OrthoDBiEOG091G01FK
PhylomeDBiO60244
TreeFamiTF314388

Family and domain databases

InterProiView protein in InterPro
IPR013947 Mediator_Med14
PfamiView protein in Pfam
PF08638 Med14, 1 hit

Sequencei

Sequence statusi: Complete.

O60244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR
60 70 80 90 100
LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFAS RTRQLFVRLL
110 120 130 140 150
ALVKWANNAG KVEKCAMISS FLDQQAILFV DTADRLASLA RDALVHARLP
160 170 180 190 200
SFAIPYAIDV LTTGSYPRLP TCIRDKIIPP DPITKIEKQA TLHQLNQILR
210 220 230 240 250
HRLVTTDLPP QLANLTVANG RVKFRVEGEF EATLTVMGDD PDVPWRLLKL
260 270 280 290 300
EILVEDKETG DGRALVHSMQ ISFIHQLVQS RLFADEKPLQ DMYNCLHSFC
310 320 330 340 350
LSLQLEVLHS QTLMLIRERW GDLVQVERYH AGKCLSLSVW NQQVLGRKTG
360 370 380 390 400
TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDHLSIEKL
410 420 430 440 450
LIDSVHARAH QKLQELKAIL RGFNANENSS IETALPALVV PILEPCGNSE
460 470 480 490 500
CLHIFVDLHS GMFQLMLYGL DQATLDDMEK SVNDDMKRII PWIQQLKFWL
510 520 530 540 550
GQQRCKQSIK HLPTISSETL QLSNYSTHPI GNLSKNKLFI KLTRLPQYYI
560 570 580 590 600
VVEMLEVPNK PTQLSYKYYF MSVNAADRED SPAMALLLQQ FKENIQDLVF
610 620 630 640 650
RTKTGKQTRT NAKRKLSDDP CPVESKKTKR AGEMCAFNKV LAHFVAMCDT
660 670 680 690 700
NMPFVGLRLE LSNLEIPHQG VQVEGDGFSH AIRLLKIPPC KGITEETQKA
710 720 730 740 750
LDRSLLDCTF RLQGRNNRTW VAELVFANCP LNGTSTREQG PSRHVYLTYE
760 770 780 790 800
NLLSEPVGGR KVVEMFLNDW NSIARLYECV LEFARSLPDI PAHLNIFSEV
810 820 830 840 850
RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT
860 870 880 890 900
ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVPM LGLTQRTNTA
910 920 930 940 950
YQCFSILPQS STHIRLAFRN MYCIDIYCRS RGVVAIRDGA YSLFDNSKLV
960 970 980 990 1000
EGFYPAPGLK TFLNMFVDSN QDARRRSVNE DDNPPSPIGG DMMDSLISQL
1010 1020 1030 1040 1050
QPPPQQQPFP KQPGTSGAYP LTSPPTSYHS TVNQSPSMMH TQSPGNLHAA
1060 1070 1080 1090 1100
SSPSGALRAP SPASFVPTPP PSSHGISIGP GASFASPHGT LDPSSPYTMV
1110 1120 1130 1140 1150
SPSGRAGNWP GSPQVSGPSP AARMPGMSPA NPSLHSPVPD ASHSPRAGTS
1160 1170 1180 1190 1200
SQTMPTNMPP PRKLPQRSWA ASIPTILTHS ALNILLLPSP TPGLVPGLAG
1210 1220 1230 1240 1250
SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR
1260 1270 1280 1290 1300
VALSPKTNQT LQLKVTPENA GQWKPDELQV LEKFFETRVA GPPFKANTLI
1310 1320 1330 1340 1350
AFTKLLGAPT HILRDCVHIM KLELFPDQAT QLKWNVQFCL TIPPSAPPIA
1360 1370 1380 1390 1400
PPGTPAVVLK SKMLFFLQLT QKTSVPPQEP VSIIVPIIYD MASGTTQQAD
1410 1420 1430 1440 1450
IPRQQNSSVA APMMVSNILK RFAEMNPPRQ GECTIFAAVR DLMANLTLPP

GGRP
Length:1,454
Mass (Da):160,607
Last modified:April 17, 2007 - v2
Checksum:i144308E3F08D3D9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309 – 310HS → LT in AAD24360 (PubMed:10024883).Curated2
Sequence conflicti1265V → L in BAA28626 (PubMed:9598311).Curated1
Sequence conflicti1265V → L in AAD12725 (PubMed:9989412).Curated1
Sequence conflicti1265V → L in AAG22547 (PubMed:10235266).Curated1
Sequence conflicti1451G → V in BAA28626 (PubMed:9598311).Curated1
Sequence conflicti1451G → V in AAD12725 (PubMed:9989412).Curated1
Sequence conflicti1451G → V in AAG22547 (PubMed:10235266).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0366081325F → L in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006651 mRNA Translation: BAA28626.1
AB006652 Genomic DNA Translation: BAA28627.1
AF104256 mRNA Translation: AAD12725.1
AF304448 mRNA Translation: AAG22547.1
AF135802 mRNA Translation: AAD24360.1
BC098377 mRNA Translation: AAH98377.1
BC132672 mRNA Translation: AAI32673.1
BC132674 mRNA Translation: AAI32675.1
CCDSiCCDS14254.1
RefSeqiNP_004220.2, NM_004229.3
UniGeneiHs.407604

Genome annotation databases

EnsembliENST00000324817; ENSP00000323720; ENSG00000180182
GeneIDi9282
KEGGihsa:9282
UCSCiuc004dex.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMED14_HUMAN
AccessioniPrimary (citable) accession number: O60244
Secondary accession number(s): Q4KMR7, Q9UNB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: April 17, 2007
Last modified: May 23, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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