O60243 (H6ST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparan-sulfate 6-O-sulfotransferase 1
Short name=HS6ST-1
EC=2.8.2.-

Gene names

Name:	HS6ST1
Synonyms:	HS6ST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed in fetal brain. Ref.1
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the sulfotransferase 6 family.
Sequence caution	The sequence AAY14736.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA25760.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA25760.1 differs from that shown. Reason: Frameshift at positions 5 and 7. The sequence BAG57153.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: Compara carbohydrate metabolic process Traceable author statement. Source: Reactome glycosaminoglycan biosynthetic process Traceable author statement. Source: Reactome heparan sulfate proteoglycan biosynthetic process, enzymatic modification Traceable author statement Ref.1. Source: ProtInc labyrinthine layer blood vessel development Inferred from electronic annotation. Source: Compara lung alveolus development Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	sulfotransferase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60243-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60243-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-162: Missing.
     163-175: VLDRRDSAALRTP → MFSWCLWPVVGES

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Heparan-sulfate 6-O-sulfotransferase 1		PRO_0000190801
Regions
	Topological domain	11 – 17	7	Cytoplasmic Potential
	Transmembrane	18 – 37	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	38 – 411	374	Lumenal Potential
	Region	96 – 102	7	5'-phosphosulfate-binding Potential
	Region	185 – 193	9	3'-phosphate binding Potential
	Coiled coil	352 – 387	36	Potential
	Compositional bias	2 – 5	4	Poly-Arg
Amino acid modifications
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 162	162	Missing in isoform 2.		VSP_037048
	Alternative sequence	163 – 175	13	VLDRR…ALRTP → MFSWCLWPVVGES in isoform 2.		VSP_037049
Experimental info
	Sequence conflict	55	1	D → Y in BAA25760. Ref.1
	Sequence conflict	191	1	Y → C in BAG57153. Ref.2
	Sequence conflict	255	1	A → T in BAG57153. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 5. Checksum: FCBF7AFAF1F3325D	FASTA	411	48,226
	10 20 30 40 50 60 MRRRRAGGRT MVERASKFVL VVAGSVCFML ILYQYAGPGL SLGAPGGRAP PDDLDLFPTP 70 80 90 100 110 120 DPHYEKKYYF PVRELERSLR FDMKGDDVIV FLHIQKTGGT TFGRHLVQNV RLEVPCDCRP 130 140 150 160 170 180 GQKKCTCYRP NRRETWLFSR FSTGWSCGLH ADWTELTNCV PGVLDRRDSA ALRTPRKFYY 190 200 210 220 230 240 ITLLRDPVSR YLSEWRHVQR GATWKTSLHM CDGRTPTPEE LPPCYEGTDW SGCTLQEFMD 250 260 270 280 290 300 CPYNLANNRQ VRMLADLSLV GCYNLSFIPE GKRAQLLLES AKKNLRGMAF FGLTEFQRKT 310 320 330 340 350 360 QYLFERTFNL KFIRPFMQYN STRAGGVEVD EDTIRRIEEL NDLDMQLYDY AKDLFQQRYQ 370 380 390 400 410 YKRQLERREQ RLRSREERLL HRAKEALPRE DADEPGRVPT EDYMSHIIEK W
	Isoform 2 [UniParc]. Checksum: 8579B4F4EE555A99	FASTA	249	29,870
	10 20 30 40 50 60 MFSWCLWPVV GESRKFYYIT LLRDPVSRYL SEWRHVQRGA TWKTSLHMCD GRTPTPEELP 70 80 90 100 110 120 PCYEGTDWSG CTLQEFMDCP YNLANNRQVR MLADLSLVGC YNLSFIPEGK RAQLLLESAK 130 140 150 160 170 180 KNLRGMAFFG LTEFQRKTQY LFERTFNLKF IRPFMQYNST RAGGVEVDED TIRRIEELND 190 200 210 220 230 240 LDMQLYDYAK DLFQQRYQYK RQLERREQRL RSREERLLHR AKEALPREDA DEPGRVPTED YMSHIIEKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and expression of heparan-sulfate 6-sulfotransferase." Habuchi H., Kobayashi M., Kimata K. J. Biol. Chem. 273:9208-9213(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-411 (ISOFORM 1). Tissue: Cerebellum and Hippocampus.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006179 mRNA. Translation: BAA25760.1. Sequence problems. AK293724 mRNA. Translation: BAG57153.1. Different initiation. AK295898 mRNA. Translation: BAG58691.1. AC017079 Genomic DNA. Translation: AAY14736.1. Different initiation. BC001196 mRNA. Translation: AAH01196.1. BC096239 mRNA. Translation: AAH96239.4. BC096240 mRNA. Translation: AAH96240.4. BC099638 mRNA. Translation: AAH99638.4. BC099639 mRNA. Translation: AAH99639.4.
IPI	IPI00028449. IPI00929423.
RefSeq	NP_004798.3. NM_004807.2.
UniGene	Hs.512841.
3D structure databases
ProteinModelPortal	O60243.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000259241.
PTM databases
PhosphoSite	O60243.
Proteomic databases
PaxDb	O60243.
PRIDE	O60243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259241; ENSP00000259241; ENSG00000136720.
GeneID	9394.
KEGG	hsa:9394.
UCSC	uc002tpt.4. human.
Organism-specific databases
CTD	9394.
GeneCards	GC02M128929.
HGNC	HGNC:5201. HS6ST1.
HPA	HPA044586.
MIM	604846. gene.
neXtProt	NX_O60243.
Orphanet	478. Kallmann syndrome. 432. Normosmic congenital hypogonadotropic hypogonadism.
PharmGKB	PA35102.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG302961.
HOGENOM	HOG000007772.
HOVERGEN	HBG083012.
InParanoid	O60243.
KO	K02514.
OMA	MVERSSK.
OrthoDB	EOG4X6C8G.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000136720-MONOMER.
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	O60243.
Bgee	O60243.
CleanEx	HS_HS6ST1.
Genevestigator	O60243.
GermOnline	ENSG00000136720. Homo sapiens.
Family and domain databases
InterPro	IPR010635. Heparan_SO4-6-sulfoTrfase. IPR005331. Sulfotransferase. [Graphical view]
PANTHER	PTHR12812. PTHR12812. 1 hit.
Pfam	PF03567. Sulfotransfer_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HS6ST1. human.
GenomeRNAi	9394.
NextBio	35195.
SOURCE	Search...

Entry information

Entry name

H6ST1_HUMAN

Accession

Primary (citable) accession number: O60243
Secondary accession number(s): B4DEP2 , B4DJ29, Q53SL2, Q9BVI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 91 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.