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Protein

SH3 domain-binding protein 5

Gene

SH3BP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.2 Publications

GO - Molecular functioni

  • protein kinase inhibitor activity Source: MGI
  • SH3 domain binding Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: MGI
  • negative regulation of protein tyrosine kinase activity Source: GO_Central
  • signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131370-MONOMER.
SignaLinkiO60239.
SIGNORiO60239.

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 5
Short name:
SH3BP-5
Alternative name(s):
SH3 domain-binding protein that preferentially associates with BTK
Gene namesi
Name:SH3BP5
Synonyms:SAB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10827. SH3BP5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi347L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. 1 Publication1
Mutagenesisi349L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. 1 Publication1
Mutagenesisi434L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. 1 Publication1
Mutagenesisi436L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. 1 Publication1

Organism-specific databases

DisGeNETi9467.
OpenTargetsiENSG00000131370.
PharmGKBiPA35735.

Polymorphism and mutation databases

BioMutaiSH3BP5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643681 – 455SH3 domain-binding protein 5Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei351Phosphoserine; by MAPK12 and MAPK9By similarity1
Modified residuei375PhosphoserineBy similarity1
Modified residuei376PhosphoserineBy similarity1
Modified residuei418PhosphoserineBy similarity1
Modified residuei421PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60239.
PaxDbiO60239.
PeptideAtlasiO60239.
PRIDEiO60239.
TopDownProteomicsiO60239-1. [O60239-1]

PTM databases

iPTMnetiO60239.
PhosphoSitePlusiO60239.

Expressioni

Tissue specificityi

Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.1 Publication

Gene expression databases

BgeeiENSG00000131370.
CleanExiHS_SH3BP5.
ExpressionAtlasiO60239. baseline and differential.
GenevisibleiO60239. HS.

Organism-specific databases

HPAiHPA036445.

Interactioni

Subunit structurei

Interacts with BTK (PubMed:9571151, PubMed:10339589). Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12 (PubMed:12167088). Interacts with GDP-bound and nucleotide-free forms of RAB11A (PubMed:26506309).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BTKQ061874EBI-624860,EBI-624835

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114853. 21 interactors.
IntActiO60239. 10 interactors.
STRINGi9606.ENSP00000373301.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H3BX-ray2.08B/D341-350[»]
ProteinModelPortaliO60239.
SMRiO60239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 67Glu-richAdd BLAST57
Compositional biasi36 – 40Poly-Glu5
Compositional biasi278 – 421Ser-richAdd BLAST144
Compositional biasi405 – 410Poly-Ser6

Sequence similaritiesi

Belongs to the SH3BP5 family.Curated

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG2008. Eukaryota.
ENOG410ZK48. LUCA.
GeneTreeiENSGT00390000018500.
HOGENOMiHOG000212681.
HOVERGENiHBG018168.
InParanoidiO60239.
OMAiPECEAER.
OrthoDBiEOG091G08YD.
PhylomeDBiO60239.
TreeFamiTF105573.

Family and domain databases

InterProiIPR007940. SH3BP5.
[Graphical view]
PANTHERiPTHR19423. PTHR19423. 1 hit.
PfamiPF05276. SH3BP5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60239-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE
60 70 80 90 100
KLNQSTDDIN RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP
110 120 130 140 150
YWEARRVARQ AQLEAQKATQ DFQRATEVLR AAKETISLAE QRLLEDDKRQ
160 170 180 190 200
FDSAWQEMLN HATQRVMEAE QTKTRSELVH KETAARYNAA MGRMRQLEKK
210 220 230 240 250
LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK GEYKMALKNL
260 270 280 290 300
EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE
310 320 330 340 350
AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP
360 370 380 390 400
SPVSLSEFGM MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN
410 420 430 440 450
NRGLSSSSGS GGSSKSQSST SPEGQALENR MKQLSLQCSK GRDGIIADIK

MVQIG
Length:455
Mass (Da):50,425
Last modified:January 23, 2007 - v2
Checksum:i5C8CA4861D66346B
GO
Isoform 2 (identifier: O60239-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: No experimental confirmation available.
Show »
Length:298
Mass (Da):32,202
Checksum:i4326AF41042E6F58
GO

Sequence cautioni

The sequence BAA25922 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0428541 – 157Missing in isoform 2. 1 PublicationAdd BLAST157

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090524 mRNA. Translation: BAG52178.1.
AC087590 Genomic DNA. No translation available.
AL117422 mRNA. Translation: CAI46220.1.
CH471055 Genomic DNA. Translation: EAW64225.1.
BC010123 mRNA. Translation: AAH10123.2.
AB005047 mRNA. Translation: BAA25922.1. Different initiation.
CCDSiCCDS2625.2. [O60239-1]
CCDS43055.1. [O60239-2]
PIRiJE0086.
RefSeqiNP_001018009.2. NM_001018009.3. [O60239-2]
NP_004835.2. NM_004844.4. [O60239-1]
XP_016863011.1. XM_017007522.1. [O60239-2]
XP_016863012.1. XM_017007523.1. [O60239-2]
XP_016863013.1. XM_017007524.1. [O60239-2]
XP_016863014.1. XM_017007525.1. [O60239-2]
UniGeneiHs.257761.

Genome annotation databases

EnsembliENST00000383791; ENSP00000373301; ENSG00000131370. [O60239-1]
ENST00000408919; ENSP00000386231; ENSG00000131370. [O60239-2]
ENST00000426925; ENSP00000388553; ENSG00000131370. [O60239-2]
GeneIDi9467.
KEGGihsa:9467.
UCSCiuc003bzp.3. human. [O60239-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090524 mRNA. Translation: BAG52178.1.
AC087590 Genomic DNA. No translation available.
AL117422 mRNA. Translation: CAI46220.1.
CH471055 Genomic DNA. Translation: EAW64225.1.
BC010123 mRNA. Translation: AAH10123.2.
AB005047 mRNA. Translation: BAA25922.1. Different initiation.
CCDSiCCDS2625.2. [O60239-1]
CCDS43055.1. [O60239-2]
PIRiJE0086.
RefSeqiNP_001018009.2. NM_001018009.3. [O60239-2]
NP_004835.2. NM_004844.4. [O60239-1]
XP_016863011.1. XM_017007522.1. [O60239-2]
XP_016863012.1. XM_017007523.1. [O60239-2]
XP_016863013.1. XM_017007524.1. [O60239-2]
XP_016863014.1. XM_017007525.1. [O60239-2]
UniGeneiHs.257761.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H3BX-ray2.08B/D341-350[»]
ProteinModelPortaliO60239.
SMRiO60239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114853. 21 interactors.
IntActiO60239. 10 interactors.
STRINGi9606.ENSP00000373301.

PTM databases

iPTMnetiO60239.
PhosphoSitePlusiO60239.

Polymorphism and mutation databases

BioMutaiSH3BP5.

Proteomic databases

MaxQBiO60239.
PaxDbiO60239.
PeptideAtlasiO60239.
PRIDEiO60239.
TopDownProteomicsiO60239-1. [O60239-1]

Protocols and materials databases

DNASUi9467.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000383791; ENSP00000373301; ENSG00000131370. [O60239-1]
ENST00000408919; ENSP00000386231; ENSG00000131370. [O60239-2]
ENST00000426925; ENSP00000388553; ENSG00000131370. [O60239-2]
GeneIDi9467.
KEGGihsa:9467.
UCSCiuc003bzp.3. human. [O60239-1]

Organism-specific databases

CTDi9467.
DisGeNETi9467.
GeneCardsiSH3BP5.
H-InvDBHIX0003099.
HGNCiHGNC:10827. SH3BP5.
HPAiHPA036445.
MIMi605612. gene.
neXtProtiNX_O60239.
OpenTargetsiENSG00000131370.
PharmGKBiPA35735.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2008. Eukaryota.
ENOG410ZK48. LUCA.
GeneTreeiENSGT00390000018500.
HOGENOMiHOG000212681.
HOVERGENiHBG018168.
InParanoidiO60239.
OMAiPECEAER.
OrthoDBiEOG091G08YD.
PhylomeDBiO60239.
TreeFamiTF105573.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131370-MONOMER.
SignaLinkiO60239.
SIGNORiO60239.

Miscellaneous databases

ChiTaRSiSH3BP5. human.
GeneWikiiSH3BP5.
GenomeRNAii9467.
PROiO60239.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131370.
CleanExiHS_SH3BP5.
ExpressionAtlasiO60239. baseline and differential.
GenevisibleiO60239. HS.

Family and domain databases

InterProiIPR007940. SH3BP5.
[Graphical view]
PANTHERiPTHR19423. PTHR19423. 1 hit.
PfamiPF05276. SH3BP5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3BP5_HUMAN
AccessioniPrimary (citable) accession number: O60239
Secondary accession number(s): B3KQW6, Q5JWV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.