O60239 (3BP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SH3 domain-binding protein 5
Short name=SH3BP-5

Alternative name(s):
SH3 domain-binding protein that preferentially associates with BTK

Gene names

Name:	SH3BP5
Synonyms:	SAB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. Ref.3 Ref.4
Subunit structure	Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12. Ref.3 Ref.4 Ref.5
Subcellular location	Mitochondrion Ref.5.
Tissue specificity	Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate. Ref.3
Sequence similarities	Belongs to the SH3BP5 family.
Sequence caution	The sequence BAA25922.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	SH3-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from direct assay Ref.4. Source: MGI
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase inhibitor activity Inferred from direct assay Ref.4. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BTK	Q06187	4	EBI-624860,EBI-624835

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	SH3 domain-binding protein 5		PRO_0000064368
Regions
	Compositional bias	11 – 67	57	Glu-rich
	Compositional bias	36 – 40	5	Poly-Glu
	Compositional bias	278 – 421	144	Ser-rich
	Compositional bias	405 – 410	6	Poly-Ser
Amino acid modifications
	Modified residue	351	1	Phosphoserine; by MAPK12 and MAPK9 By similarity
	Modified residue	418	1	Phosphoserine By similarity
	Modified residue	421	1	Phosphoserine Ref.6 Ref.7
Experimental info
	Mutagenesis	347	1	L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. Ref.5
	Mutagenesis	349	1	L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. Ref.5
	Mutagenesis	434	1	L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. Ref.5
	Mutagenesis	436	1	L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O60239 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5C8CA4861D66346B	FASTA	455	50,425
	10 20 30 40 50 60 MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE KLNQSTDDIN 70 80 90 100 110 120 RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP YWEARRVARQ AQLEAQKATQ 130 140 150 160 170 180 DFQRATEVLR AAKETISLAE QRLLEDDKRQ FDSAWQEMLN HATQRVMEAE QTKTRSELVH 190 200 210 220 230 240 KETAARYNAA MGRMRQLEKK LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK 250 260 270 280 290 300 GEYKMALKNL EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE 310 320 330 340 350 360 AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP SPVSLSEFGM 370 380 390 400 410 420 MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN NRGLSSSSGS GGSSKSQSST 430 440 450 SPEGQALENR MKQLSLQCSK GRDGIIADIK MVQIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	"Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)." Matsushita M., Yamadori T., Kato S., Takemoto Y., Inazawa J., Baba Y., Hashimoto S., Sekine S., Arai S., Kunikata T., Kurimoto M., Kishimoto T., Tsukada S. Biochem. Biophys. Res. Commun. 245:337-343(1998) [PubMed: 9571151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-455, FUNCTION, INTERACTION WITH BTK, TISSUE SPECIFICITY. Tissue: Placenta.
[4]	"Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein." Yamadori T., Baba Y., Mastushita M., Hashimoto S., Kurosaki M., Kurosaki T., Kishimoto T., Tsukada S. Proc. Natl. Acad. Sci. U.S.A. 96:6341-6346(1999) [PubMed: 10339589] [Abstract] Cited for: INTERACTION WITH BTK, FUNCTION.
[5]	"A new c-Jun N-terminal kinase (JNK)-interacting protein, Sab (SH3BP5), associates with mitochondria." Wiltshire C., Matsushita M., Tsukada S., Gillespie D.A., May G.H. Biochem. J. 367:577-585(2002) [PubMed: 12167088] [Abstract] Cited for: INTERACTION WITH MAPK8; MAPK9 AND MAPK10, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-347; LEU-349; LEU-434 AND LEU-436.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL117422 mRNA. Translation: CAI46220.1. BC010123 mRNA. Translation: AAH10123.2. AB005047 mRNA. Translation: BAA25922.1. Different initiation.
IPI	IPI00744182.
PIR	JE0086.
RefSeq	NP_004835.2. NM_004844.3.
UniGene	Hs.257761. Hs.726063.
3D structure databases
ProteinModelPortal	O60239.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60239. 6 interactions.
STRING	O60239.
PTM databases
PhosphoSite	O60239.
Proteomic databases
PRIDE	O60239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000383791; ENSP00000373301; ENSG00000131370.
GeneID	9467.
KEGG	hsa:9467.
UCSC	uc003bzp.1. human.
Organism-specific databases
CTD	9467.
GeneCards	GC03M015296.
H-InvDB	HIX0003100.
HGNC	HGNC:10827. SH3BP5.
HPA	HPA036445.
MIM	605612. gene.
neXtProt	NX_O60239.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10117.
HOGENOM	HBG716674.
HOVERGEN	HBG018168.
InParanoid	O60239.
OMA	KYNAAMG.
OrthoDB	EOG4Q84XV.
PhylomeDB	O60239.
Enzyme and pathway databases
Pathway_Interaction_DB	bcr_5pathway. BCR signaling pathway.
Gene expression databases
ArrayExpress	O60239.
Bgee	O60239.
CleanEx	HS_SH3BP5.
Genevestigator	O60239.
GermOnline	ENSG00000131370. Homo sapiens.
Family and domain databases
InterPro	IPR007940. SH3-bd_5. [Graphical view]
PANTHER	PTHR19423. SH3_bd_5. 1 hit.
Pfam	PF05276. SH3BP5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	35476.
SOURCE	Search...

Entry information

Entry name

3BP5_HUMAN

Accession

Primary (citable) accession number: O60239
Secondary accession number(s): Q5JWV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.