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O60239

- 3BP5_HUMAN

UniProt

O60239 - 3BP5_HUMAN

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Protein

SH3 domain-binding protein 5

Gene

SH3BP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.2 Publications

GO - Molecular functioni

  1. protein kinase inhibitor activity Source: MGI

GO - Biological processi

  1. intracellular signal transduction Source: MGI
  2. signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiO60239.

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 5
Short name:
SH3BP-5
Alternative name(s):
SH3 domain-binding protein that preferentially associates with BTK
Gene namesi
Name:SH3BP5
Synonyms:SAB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:10827. SH3BP5.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi347 – 3471L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. 1 Publication
Mutagenesisi349 – 3491L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. 1 Publication
Mutagenesisi434 – 4341L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. 1 Publication
Mutagenesisi436 – 4361L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. 1 Publication

Organism-specific databases

PharmGKBiPA35735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455SH3 domain-binding protein 5PRO_0000064368Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei351 – 3511Phosphoserine; by MAPK12 and MAPK9By similarity
Modified residuei421 – 4211Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60239.
PaxDbiO60239.
PRIDEiO60239.

PTM databases

PhosphoSiteiO60239.

Expressioni

Tissue specificityi

Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.1 Publication

Gene expression databases

BgeeiO60239.
CleanExiHS_SH3BP5.
ExpressionAtlasiO60239. baseline and differential.
GenevestigatoriO60239.

Organism-specific databases

HPAiHPA036445.

Interactioni

Subunit structurei

Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BTKQ061874EBI-624860,EBI-624835

Protein-protein interaction databases

BioGridi114853. 17 interactions.
IntActiO60239. 6 interactions.
STRINGi9606.ENSP00000373301.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H3BX-ray2.08B/D341-350[»]
ProteinModelPortaliO60239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 6757Glu-richAdd
BLAST
Compositional biasi36 – 405Poly-Glu
Compositional biasi278 – 421144Ser-richAdd
BLAST
Compositional biasi405 – 4106Poly-Ser

Sequence similaritiesi

Belongs to the SH3BP5 family.Curated

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiNOG263345.
GeneTreeiENSGT00390000018500.
HOGENOMiHOG000212681.
HOVERGENiHBG018168.
InParanoidiO60239.
OMAiNNRGLSN.
OrthoDBiEOG75F4FK.
PhylomeDBiO60239.
TreeFamiTF105573.

Family and domain databases

InterProiIPR007940. SH3-bd_5.
[Graphical view]
PANTHERiPTHR19423. PTHR19423. 1 hit.
PfamiPF05276. SH3BP5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O60239-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE
60 70 80 90 100
KLNQSTDDIN RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP
110 120 130 140 150
YWEARRVARQ AQLEAQKATQ DFQRATEVLR AAKETISLAE QRLLEDDKRQ
160 170 180 190 200
FDSAWQEMLN HATQRVMEAE QTKTRSELVH KETAARYNAA MGRMRQLEKK
210 220 230 240 250
LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK GEYKMALKNL
260 270 280 290 300
EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE
310 320 330 340 350
AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP
360 370 380 390 400
SPVSLSEFGM MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN
410 420 430 440 450
NRGLSSSSGS GGSSKSQSST SPEGQALENR MKQLSLQCSK GRDGIIADIK

MVQIG
Length:455
Mass (Da):50,425
Last modified:January 23, 2007 - v2
Checksum:i5C8CA4861D66346B
GO
Isoform 2 (identifier: O60239-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: No experimental confirmation available.

Show »
Length:298
Mass (Da):32,202
Checksum:i4326AF41042E6F58
GO

Sequence cautioni

The sequence BAA25922.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 157157Missing in isoform 2. 1 PublicationVSP_042854Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK090524 mRNA. Translation: BAG52178.1.
AC087590 Genomic DNA. No translation available.
AL117422 mRNA. Translation: CAI46220.1.
CH471055 Genomic DNA. Translation: EAW64225.1.
BC010123 mRNA. Translation: AAH10123.2.
AB005047 mRNA. Translation: BAA25922.1. Different initiation.
CCDSiCCDS2625.2. [O60239-1]
CCDS43055.1. [O60239-2]
PIRiJE0086.
RefSeqiNP_001018009.2. NM_001018009.3. [O60239-2]
NP_004835.2. NM_004844.4. [O60239-1]
XP_006713483.1. XM_006713420.1. [O60239-2]
UniGeneiHs.257761.

Genome annotation databases

EnsembliENST00000383791; ENSP00000373301; ENSG00000131370. [O60239-1]
ENST00000408919; ENSP00000386231; ENSG00000131370. [O60239-2]
ENST00000426925; ENSP00000388553; ENSG00000131370. [O60239-2]
GeneIDi9467.
KEGGihsa:9467.
UCSCiuc003bzp.2. human. [O60239-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK090524 mRNA. Translation: BAG52178.1 .
AC087590 Genomic DNA. No translation available.
AL117422 mRNA. Translation: CAI46220.1 .
CH471055 Genomic DNA. Translation: EAW64225.1 .
BC010123 mRNA. Translation: AAH10123.2 .
AB005047 mRNA. Translation: BAA25922.1 . Different initiation.
CCDSi CCDS2625.2. [O60239-1 ]
CCDS43055.1. [O60239-2 ]
PIRi JE0086.
RefSeqi NP_001018009.2. NM_001018009.3. [O60239-2 ]
NP_004835.2. NM_004844.4. [O60239-1 ]
XP_006713483.1. XM_006713420.1. [O60239-2 ]
UniGenei Hs.257761.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4H3B X-ray 2.08 B/D 341-350 [» ]
ProteinModelPortali O60239.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114853. 17 interactions.
IntActi O60239. 6 interactions.
STRINGi 9606.ENSP00000373301.

PTM databases

PhosphoSitei O60239.

Proteomic databases

MaxQBi O60239.
PaxDbi O60239.
PRIDEi O60239.

Protocols and materials databases

DNASUi 9467.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000383791 ; ENSP00000373301 ; ENSG00000131370 . [O60239-1 ]
ENST00000408919 ; ENSP00000386231 ; ENSG00000131370 . [O60239-2 ]
ENST00000426925 ; ENSP00000388553 ; ENSG00000131370 . [O60239-2 ]
GeneIDi 9467.
KEGGi hsa:9467.
UCSCi uc003bzp.2. human. [O60239-1 ]

Organism-specific databases

CTDi 9467.
GeneCardsi GC03M015296.
H-InvDB HIX0003099.
HGNCi HGNC:10827. SH3BP5.
HPAi HPA036445.
MIMi 605612. gene.
neXtProti NX_O60239.
PharmGKBi PA35735.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG263345.
GeneTreei ENSGT00390000018500.
HOGENOMi HOG000212681.
HOVERGENi HBG018168.
InParanoidi O60239.
OMAi NNRGLSN.
OrthoDBi EOG75F4FK.
PhylomeDBi O60239.
TreeFami TF105573.

Enzyme and pathway databases

SignaLinki O60239.

Miscellaneous databases

GeneWikii SH3BP5.
GenomeRNAii 9467.
NextBioi 35476.
PROi O60239.
SOURCEi Search...

Gene expression databases

Bgeei O60239.
CleanExi HS_SH3BP5.
ExpressionAtlasi O60239. baseline and differential.
Genevestigatori O60239.

Family and domain databases

InterProi IPR007940. SH3-bd_5.
[Graphical view ]
PANTHERi PTHR19423. PTHR19423. 1 hit.
Pfami PF05276. SH3BP5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Adrenal gland.
  3. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  6. "Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)."
    Matsushita M., Yamadori T., Kato S., Takemoto Y., Inazawa J., Baba Y., Hashimoto S., Sekine S., Arai S., Kunikata T., Kurimoto M., Kishimoto T., Tsukada S.
    Biochem. Biophys. Res. Commun. 245:337-343(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-455 (ISOFORM 1), FUNCTION, INTERACTION WITH BTK, TISSUE SPECIFICITY.
    Tissue: Placenta.
  7. "Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein."
    Yamadori T., Baba Y., Mastushita M., Hashimoto S., Kurosaki M., Kurosaki T., Kishimoto T., Tsukada S.
    Proc. Natl. Acad. Sci. U.S.A. 96:6341-6346(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BTK, FUNCTION.
  8. "A new c-Jun N-terminal kinase (JNK)-interacting protein, Sab (SH3BP5), associates with mitochondria."
    Wiltshire C., Matsushita M., Tsukada S., Gillespie D.A., May G.H.
    Biochem. J. 367:577-585(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAPK8; MAPK9 AND MAPK10, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-347; LEU-349; LEU-434 AND LEU-436.
  9. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry namei3BP5_HUMAN
AccessioniPrimary (citable) accession number: O60239
Secondary accession number(s): B3KQW6, Q5JWV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3