O60239 (3BP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SH3 domain-binding protein 5
Short name=SH3BP-5

Alternative name(s):
SH3 domain-binding protein that preferentially associates with BTK

Gene names

Name:	SH3BP5
Synonyms:	SAB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. Ref.6 Ref.7
Subunit structure	Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12. Ref.6 Ref.7 Ref.8
Subcellular location	Mitochondrion Ref.8.
Tissue specificity	Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate. Ref.6
Sequence similarities	Belongs to the SH3BP5 family.
Sequence caution	The sequence BAA25922.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	SH3-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from direct assay Ref.7. Source: MGI
Cellular_component	cytoplasm Traceable author statement Ref.6. Source: ProtInc mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein kinase inhibitor activity Inferred from direct assay Ref.7. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BTK	Q06187	4	EBI-624860,EBI-624835

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60239-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60239-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	SH3 domain-binding protein 5		PRO_0000064368
Regions
	Compositional bias	11 – 67	57	Glu-rich
	Compositional bias	36 – 40	5	Poly-Glu
	Compositional bias	278 – 421	144	Ser-rich
	Compositional bias	405 – 410	6	Poly-Ser
Amino acid modifications
	Modified residue	351	1	Phosphoserine; by MAPK12 and MAPK9 By similarity
	Modified residue	418	1	Phosphoserine By similarity
	Modified residue	421	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	1 – 157	157	Missing in isoform 2.		VSP_042854
Experimental info
	Mutagenesis	347	1	L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. Ref.8
	Mutagenesis	349	1	L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. Ref.8
	Mutagenesis	434	1	L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. Ref.8
	Mutagenesis	436	1	L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5C8CA4861D66346B	FASTA	455	50,425
	10 20 30 40 50 60 MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE KLNQSTDDIN 70 80 90 100 110 120 RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP YWEARRVARQ AQLEAQKATQ 130 140 150 160 170 180 DFQRATEVLR AAKETISLAE QRLLEDDKRQ FDSAWQEMLN HATQRVMEAE QTKTRSELVH 190 200 210 220 230 240 KETAARYNAA MGRMRQLEKK LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK 250 260 270 280 290 300 GEYKMALKNL EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE 310 320 330 340 350 360 AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP SPVSLSEFGM 370 380 390 400 410 420 MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN NRGLSSSSGS GGSSKSQSST 430 440 450 SPEGQALENR MKQLSLQCSK GRDGIIADIK MVQIG
	Isoform 2 [UniParc]. Checksum: 4326AF41042E6F58	FASTA	298	32,202
	10 20 30 40 50 60 MLNHATQRVM EAEQTKTRSE LVHKETAARY NAAMGRMRQL EKKLKRAINK SKPYFELKAK 70 80 90 100 110 120 YYVQLEQLKK TVDDLQAKLT LAKGEYKMAL KNLEMISDEI HERRRSSAMG PRGCGVGAEG 130 140 150 160 170 180 SSTSVEDLPG SKPEPDAISV ASEAFEDDSC SNFVSEDDSE TQSVSSFSSG PTSPSEMPDQ 190 200 210 220 230 240 FPAVVRPGSL DLPSPVSLSE FGMMFPVLGP RSECSGASSP ECEVERGDRA EGAENKTSDK 250 260 270 280 290 ANNNRGLSSS SGSGGSSKSQ SSTSPEGQAL ENRMKQLSLQ CSKGRDGIIA DIKMVQIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Adrenal gland.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)." Matsushita M., Yamadori T., Kato S., Takemoto Y., Inazawa J., Baba Y., Hashimoto S., Sekine S., Arai S., Kunikata T., Kurimoto M., Kishimoto T., Tsukada S. Biochem. Biophys. Res. Commun. 245:337-343(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-455 (ISOFORM 1), FUNCTION, INTERACTION WITH BTK, TISSUE SPECIFICITY. Tissue: Placenta.
[7]	"Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein." Yamadori T., Baba Y., Mastushita M., Hashimoto S., Kurosaki M., Kurosaki T., Kishimoto T., Tsukada S. Proc. Natl. Acad. Sci. U.S.A. 96:6341-6346(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BTK, FUNCTION.
[8]	"A new c-Jun N-terminal kinase (JNK)-interacting protein, Sab (SH3BP5), associates with mitochondria." Wiltshire C., Matsushita M., Tsukada S., Gillespie D.A., May G.H. Biochem. J. 367:577-585(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAPK8; MAPK9 AND MAPK10, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-347; LEU-349; LEU-434 AND LEU-436.
[9]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK090524 mRNA. Translation: BAG52178.1.
AC087590 Genomic DNA. No translation available.
AL117422 mRNA. Translation: CAI46220.1.
CH471055 Genomic DNA. Translation: EAW64225.1.
BC010123 mRNA. Translation: AAH10123.2.
AB005047 mRNA. Translation: BAA25922.1. Different initiation.

IPI

IPI00744182.
IPI00914942.

PIR

JE0086.

RefSeq

NP_001018009.2. NM_001018009.3.
NP_004835.2. NM_004844.4.

UniGene

Hs.257761.
Hs.726063.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4H3B	X-ray	2.08	B/D	341-350	[»]

ProteinModelPortal

O60239.

ModBase

Search...

Protein-protein interaction databases

IntAct

O60239. 6 interactions.

STRING

9606.ENSP00000373301.

PTM databases

PhosphoSite

O60239.

Proteomic databases

PaxDb

O60239.

PRIDE

O60239.

Protocols and materials databases

DNASU

9467.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000253688; ENSP00000253688; ENSG00000131370.
ENST00000383791; ENSP00000373301; ENSG00000131370.
ENST00000408919; ENSP00000386231; ENSG00000131370.
ENST00000426925; ENSP00000388553; ENSG00000131370.

GeneID

9467.

KEGG

hsa:9467.

UCSC

uc003bzp.1. human.

Organism-specific databases

CTD

9467.

GeneCards

GC03M015296.

H-InvDB

HIX0003099.

HGNC

HGNC:10827. SH3BP5.

HPA

HPA036445.

MIM

605612. gene.

neXtProt

NX_O60239.

PharmGKB

PA35735.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG263345.

HOGENOM

HOG000212681.

HOVERGEN

HBG018168.

InParanoid

O60239.

OMA

NNRGLSN.

OrthoDB

EOG4Q84XV.

Enzyme and pathway databases

Pathway_Interaction_DB

bcr_5pathway. BCR signaling pathway.

Gene expression databases

ArrayExpress

O60239.

Bgee

O60239.

CleanEx

HS_SH3BP5.

Genevestigator

O60239.

GermOnline

ENSG00000131370. Homo sapiens.

Family and domain databases

InterPro

IPR007940. SH3-bd_5.
[Graphical view]

PANTHER

PTHR19423. PTHR19423. 1 hit.

Pfam

PF05276. SH3BP5. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

9467.

NextBio

35476.

SOURCE

Search...

Entry information

Entry name

3BP5_HUMAN

Accession

Primary (citable) accession number: O60239
Secondary accession number(s): B3KQW6, Q5JWV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.