Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O60239

- 3BP5_HUMAN

UniProt

O60239 - 3BP5_HUMAN

Protein

SH3 domain-binding protein 5

Gene

SH3BP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase inhibitor activity Source: MGI

    GO - Biological processi

    1. intracellular signal transduction Source: MGI
    2. signal transduction Source: ProtInc

    Enzyme and pathway databases

    SignaLinkiO60239.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SH3 domain-binding protein 5
    Short name:
    SH3BP-5
    Alternative name(s):
    SH3 domain-binding protein that preferentially associates with BTK
    Gene namesi
    Name:SH3BP5
    Synonyms:SAB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:10827. SH3BP5.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi347 – 3471L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. 1 Publication
    Mutagenesisi349 – 3491L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. 1 Publication
    Mutagenesisi434 – 4341L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. 1 Publication
    Mutagenesisi436 – 4361L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. 1 Publication

    Organism-specific databases

    PharmGKBiPA35735.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455SH3 domain-binding protein 5PRO_0000064368Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei351 – 3511Phosphoserine; by MAPK12 and MAPK9By similarity
    Modified residuei421 – 4211Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO60239.
    PaxDbiO60239.
    PRIDEiO60239.

    PTM databases

    PhosphoSiteiO60239.

    Expressioni

    Tissue specificityi

    Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.1 Publication

    Gene expression databases

    ArrayExpressiO60239.
    BgeeiO60239.
    CleanExiHS_SH3BP5.
    GenevestigatoriO60239.

    Organism-specific databases

    HPAiHPA036445.

    Interactioni

    Subunit structurei

    Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BTKQ061874EBI-624860,EBI-624835

    Protein-protein interaction databases

    BioGridi114853. 16 interactions.
    IntActiO60239. 6 interactions.
    STRINGi9606.ENSP00000373301.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4H3BX-ray2.08B/D341-350[»]
    ProteinModelPortaliO60239.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 6757Glu-richAdd
    BLAST
    Compositional biasi36 – 405Poly-Glu
    Compositional biasi278 – 421144Ser-richAdd
    BLAST
    Compositional biasi405 – 4106Poly-Ser

    Sequence similaritiesi

    Belongs to the SH3BP5 family.Curated

    Keywords - Domaini

    SH3-binding

    Phylogenomic databases

    eggNOGiNOG263345.
    HOGENOMiHOG000212681.
    HOVERGENiHBG018168.
    InParanoidiO60239.
    OMAiNNRGLSN.
    OrthoDBiEOG75F4FK.
    PhylomeDBiO60239.
    TreeFamiTF105573.

    Family and domain databases

    InterProiIPR007940. SH3-bd_5.
    [Graphical view]
    PANTHERiPTHR19423. PTHR19423. 1 hit.
    PfamiPF05276. SH3BP5. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O60239-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE    50
    KLNQSTDDIN RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP 100
    YWEARRVARQ AQLEAQKATQ DFQRATEVLR AAKETISLAE QRLLEDDKRQ 150
    FDSAWQEMLN HATQRVMEAE QTKTRSELVH KETAARYNAA MGRMRQLEKK 200
    LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK GEYKMALKNL 250
    EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE 300
    AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP 350
    SPVSLSEFGM MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN 400
    NRGLSSSSGS GGSSKSQSST SPEGQALENR MKQLSLQCSK GRDGIIADIK 450
    MVQIG 455
    Length:455
    Mass (Da):50,425
    Last modified:January 23, 2007 - v2
    Checksum:i5C8CA4861D66346B
    GO
    Isoform 2 (identifier: O60239-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-157: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:298
    Mass (Da):32,202
    Checksum:i4326AF41042E6F58
    GO

    Sequence cautioni

    The sequence BAA25922.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 157157Missing in isoform 2. 1 PublicationVSP_042854Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK090524 mRNA. Translation: BAG52178.1.
    AC087590 Genomic DNA. No translation available.
    AL117422 mRNA. Translation: CAI46220.1.
    CH471055 Genomic DNA. Translation: EAW64225.1.
    BC010123 mRNA. Translation: AAH10123.2.
    AB005047 mRNA. Translation: BAA25922.1. Different initiation.
    CCDSiCCDS2625.2. [O60239-1]
    CCDS43055.1. [O60239-2]
    PIRiJE0086.
    RefSeqiNP_001018009.2. NM_001018009.3. [O60239-2]
    NP_004835.2. NM_004844.4. [O60239-1]
    XP_006713483.1. XM_006713420.1. [O60239-2]
    UniGeneiHs.257761.

    Genome annotation databases

    EnsembliENST00000383791; ENSP00000373301; ENSG00000131370. [O60239-1]
    ENST00000408919; ENSP00000386231; ENSG00000131370. [O60239-2]
    ENST00000426925; ENSP00000388553; ENSG00000131370. [O60239-2]
    GeneIDi9467.
    KEGGihsa:9467.
    UCSCiuc003bzp.2. human. [O60239-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK090524 mRNA. Translation: BAG52178.1 .
    AC087590 Genomic DNA. No translation available.
    AL117422 mRNA. Translation: CAI46220.1 .
    CH471055 Genomic DNA. Translation: EAW64225.1 .
    BC010123 mRNA. Translation: AAH10123.2 .
    AB005047 mRNA. Translation: BAA25922.1 . Different initiation.
    CCDSi CCDS2625.2. [O60239-1 ]
    CCDS43055.1. [O60239-2 ]
    PIRi JE0086.
    RefSeqi NP_001018009.2. NM_001018009.3. [O60239-2 ]
    NP_004835.2. NM_004844.4. [O60239-1 ]
    XP_006713483.1. XM_006713420.1. [O60239-2 ]
    UniGenei Hs.257761.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4H3B X-ray 2.08 B/D 341-350 [» ]
    ProteinModelPortali O60239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114853. 16 interactions.
    IntActi O60239. 6 interactions.
    STRINGi 9606.ENSP00000373301.

    PTM databases

    PhosphoSitei O60239.

    Proteomic databases

    MaxQBi O60239.
    PaxDbi O60239.
    PRIDEi O60239.

    Protocols and materials databases

    DNASUi 9467.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000383791 ; ENSP00000373301 ; ENSG00000131370 . [O60239-1 ]
    ENST00000408919 ; ENSP00000386231 ; ENSG00000131370 . [O60239-2 ]
    ENST00000426925 ; ENSP00000388553 ; ENSG00000131370 . [O60239-2 ]
    GeneIDi 9467.
    KEGGi hsa:9467.
    UCSCi uc003bzp.2. human. [O60239-1 ]

    Organism-specific databases

    CTDi 9467.
    GeneCardsi GC03M015296.
    H-InvDB HIX0003099.
    HGNCi HGNC:10827. SH3BP5.
    HPAi HPA036445.
    MIMi 605612. gene.
    neXtProti NX_O60239.
    PharmGKBi PA35735.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG263345.
    HOGENOMi HOG000212681.
    HOVERGENi HBG018168.
    InParanoidi O60239.
    OMAi NNRGLSN.
    OrthoDBi EOG75F4FK.
    PhylomeDBi O60239.
    TreeFami TF105573.

    Enzyme and pathway databases

    SignaLinki O60239.

    Miscellaneous databases

    GeneWikii SH3BP5.
    GenomeRNAii 9467.
    NextBioi 35476.
    PROi O60239.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60239.
    Bgeei O60239.
    CleanExi HS_SH3BP5.
    Genevestigatori O60239.

    Family and domain databases

    InterProi IPR007940. SH3-bd_5.
    [Graphical view ]
    PANTHERi PTHR19423. PTHR19423. 1 hit.
    Pfami PF05276. SH3BP5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Uterus.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Adrenal gland.
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    6. "Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)."
      Matsushita M., Yamadori T., Kato S., Takemoto Y., Inazawa J., Baba Y., Hashimoto S., Sekine S., Arai S., Kunikata T., Kurimoto M., Kishimoto T., Tsukada S.
      Biochem. Biophys. Res. Commun. 245:337-343(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-455 (ISOFORM 1), FUNCTION, INTERACTION WITH BTK, TISSUE SPECIFICITY.
      Tissue: Placenta.
    7. "Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein."
      Yamadori T., Baba Y., Mastushita M., Hashimoto S., Kurosaki M., Kurosaki T., Kishimoto T., Tsukada S.
      Proc. Natl. Acad. Sci. U.S.A. 96:6341-6346(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BTK, FUNCTION.
    8. "A new c-Jun N-terminal kinase (JNK)-interacting protein, Sab (SH3BP5), associates with mitochondria."
      Wiltshire C., Matsushita M., Tsukada S., Gillespie D.A., May G.H.
      Biochem. J. 367:577-585(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPK8; MAPK9 AND MAPK10, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-347; LEU-349; LEU-434 AND LEU-436.
    9. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry namei3BP5_HUMAN
    AccessioniPrimary (citable) accession number: O60239
    Secondary accession number(s): B3KQW6, Q5JWV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3