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Protein

Protein phosphatase 1 regulatory subunit 12B

Gene

PPP1R12B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates myosin phosphatase activity. Augments Ca2+ sensitivity of the contractile apparatus.2 Publications

GO - Molecular functioni

  • enzyme activator activity Source: ProtInc
  • phosphatase regulator activity Source: InterPro

GO - Biological processi

  • G2/M transition of mitotic cell cycle Source: Reactome
  • regulation of muscle contraction Source: ProtInc
  • signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BRENDAi3.1.3.53. 2681.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.
SIGNORiO60237.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 12B
Alternative name(s):
Myosin phosphatase-targeting subunit 2
Short name:
Myosin phosphatase target subunit 2
Gene namesi
Name:PPP1R12B
Synonyms:MYPT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:7619. PPP1R12B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4660.
OpenTargetsiENSG00000077157.
PharmGKBiPA33618.

Polymorphism and mutation databases

BioMutaiPPP1R12B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670281 – 982Protein phosphatase 1 regulatory subunit 12BAdd BLAST982

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineBy similarity1
Modified residuei445PhosphothreonineBy similarity1
Modified residuei646PhosphothreonineCombined sources1
Modified residuei808PhosphothreonineBy similarity1
Modified residuei839PhosphoserineBy similarity1
Modified residuei947PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60237.
PaxDbiO60237.
PeptideAtlasiO60237.
PRIDEiO60237.

2D gel databases

UCD-2DPAGEO60237.

PTM databases

iPTMnetiO60237.
PhosphoSitePlusiO60237.

Expressioni

Tissue specificityi

Detected in skeletal muscle, fetal and adult heart, brain, placenta, kidney, spleen, thymus, pancreas and lung. Isoform 3 and isoform 4 are heart specific.2 Publications

Gene expression databases

BgeeiENSG00000077157.
CleanExiHS_PPP1R12B.
ExpressionAtlasiO60237. baseline and differential.
GenevisibleiO60237. HS.

Organism-specific databases

HPAiHPA024171.
HPA024640.

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.

Binary interactionsi

WithEntry#Exp.IntActNotes
PIK3R3Q925692EBI-10700351,EBI-79893

Protein-protein interaction databases

BioGridi110743. 24 interactors.
IntActiO60237. 16 interactors.
STRINGi9606.ENSP00000384496.

Structurei

3D structure databases

ProteinModelPortaliO60237.
SMRiO60237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati90 – 119ANK 2Add BLAST30
Repeati123 – 152ANK 3Add BLAST30
Repeati216 – 245ANK 4Add BLAST30
Repeati249 – 278ANK 5Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi336 – 379Glu-richAdd BLAST44

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiO60237.
KOiK12329.
OMAiSTEKHEP.
OrthoDBiEOG091G0UPK.
PhylomeDBiO60237.
TreeFamiTF105543.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60237-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAELEHLGGK RAESARMRRA EQLRRWRGSL TEQEPAERRG AGRQPLTRRG
60 70 80 90 100
SPRVRFEDGA VFLAACSSGD TDEVRKLLAR GADINTVNVD GLTALHQACI
110 120 130 140 150
DENLDMVKFL VENRANVNQQ DNEGWTPLHA AASCGYLNIA EYFINHGASV
160 170 180 190 200
GIVNSEGEVP SDLAEEPAMK DLLLEQVKKQ GVDLEQSRKE EEQQMLQDAR
210 220 230 240 250
QWLNSGKIED VRQARSGATA LHVAAAKGYS EVLRLLIQAG YELNVQDYDG
260 270 280 290 300
WTPLHAAAHW GVKEACSILA EALCDMDIRN KLGQTPFDVA DEGLVEHLEL
310 320 330 340 350
LQKKQNVLRS EKETRNKLIE SDLNSKIQSG FFKNKEKMLY EEETPKSQEM
360 370 380 390 400
EEENKESSSS SSEEEEGEDE ASESETEKEA DKKPEAFVNH SNSESKSSIT
410 420 430 440 450
EQIPAPAQNT FSASSARRFS SGLFNKPEEP KDESPSSWRL GLRKTGSHNM
460 470 480 490 500
LSEVANSREP IRDRGSSIYR SSSSPRISAL LDNKDKEREN KSYISSLAPR
510 520 530 540 550
KLNSTSDIEE KENRESAVNL VRSGSYTRQL WRDEAKGNEI PQTIAPSTYV
560 570 580 590 600
STYLKRTPHK SQADTTAEKT ADNVSSSTPL CVITNRPLPS TANGVTATPV
610 620 630 640 650
LSITGTDSSV EAREKRRSYL TPVRDEEAES LRKARSRQAR QTRRSTQGVT
660 670 680 690 700
LTDLQEAERT FSRSRAERQA QEQPREKPTD TEGLEGSPEK HEPSAVPATE
710 720 730 740 750
AGEGQQPWGR SLDEEPICHR LRCPAQPDKP TTPASPSTSR PSLYTSSHLL
760 770 780 790 800
WTNRFSVPDS ESSETTTNTT TAKEMDKNEN EEADLDEQSS KRLSIRERRR
810 820 830 840 850
PKERRRGTGI NFWTKDEDET DGSEEVKETW HERLSRLESG GSNPTTSDSY
860 870 880 890 900
GDRASARARR EAREARLATL TSRVEEDSNR DYKKLYESAL TENQKLKTKL
910 920 930 940 950
QEAQLELADI KSKLEKVAQQ KQEKTSDRSS VLEMEKRERR ALERKMSEME
960 970 980
EEMKVLTELK SDNQRLKDEN GALIRVISKL SK
Note: Produced by alternative promoter usage.
Length:982
Mass (Da):110,404
Last modified:November 28, 2006 - v2
Checksum:iA6EBB6AD4C61DD3B
GO
Isoform 2 (identifier: O60237-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-386: DKKPEA → VLFWPF
     387-982: Missing.

Note: Produced by alternative splicing of isoform 1. No experimental confirmation available.
Show »
Length:386
Mass (Da):43,260
Checksum:iC2552EEF1F03C733
GO
Isoform 3 (identifier: O60237-3) [UniParc]FASTAAdd to basket
Also known as: hHS-M21B, Heart-specific myosin light chain phosphatase small subunit B

The sequence of this isoform differs from the canonical sequence as follows:
     1-774: Missing.
     955-982: VLTELKSDNQRLKDENGALIRVISKLSK → NLHQLKQIQTLKQMNEQLQAENRALTRVVARLSESIESSDTQEL

Note: Produced by alternative splicing of isoform 4.
Show »
Length:224
Mass (Da):26,262
Checksum:i64CC09C60F7366B3
GO
Isoform 4 (identifier: O60237-4) [UniParc]FASTAAdd to basket
Also known as: hHS-M21A, Heart-specific myosin light chain phosphatase small subunit A

The sequence of this isoform differs from the canonical sequence as follows:
     1-774: Missing.

Note: Produced by alternative promoter usage.
Show »
Length:208
Mass (Da):24,338
Checksum:i0878B07EE0661A25
GO
Isoform 5 (identifier: O60237-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     487-515: ERENKSYISSLAPRKLNSTSDIEEKENRE → VQFGRVWGNSKAVFFFHENSILGTNENIF
     516-982: Missing.

Note: No experimental confirmation available.
Show »
Length:515
Mass (Da):57,501
Checksum:i8B672C86E4A1F951
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017480182V → I.2 PublicationsCorresponds to variant rs2843414dbSNPEnsembl.1
Natural variantiVAR_024177836R → K.Corresponds to variant rs3881953dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0092561 – 774Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST774
Alternative sequenceiVSP_009257381 – 386DKKPEA → VLFWPF in isoform 2. 1 Publication6
Alternative sequenceiVSP_009258387 – 982Missing in isoform 2. 1 PublicationAdd BLAST596
Alternative sequenceiVSP_043159487 – 515ERENK…KENRE → VQFGRVWGNSKAVFFFHENS ILGTNENIF in isoform 5. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_043160516 – 982Missing in isoform 5. 1 PublicationAdd BLAST467
Alternative sequenceiVSP_009259955 – 982VLTEL…SKLSK → NLHQLKQIQTLKQMNEQLQA ENRALTRVVARLSESIESSD TQEL in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003062 mRNA. Translation: BAA28376.1.
AF324888 mRNA. Translation: AAK00337.1.
AF324892
, AF324889, AF324890, AF324891 Genomic DNA. Translation: AAK00336.1.
AB050641 mRNA. Translation: BAB17610.1.
AB050642 mRNA. Translation: BAB17611.1.
AL589762, AC099336, AL356953 Genomic DNA. Translation: CAH73375.1.
AL589762, AC099336, AL356953 Genomic DNA. Translation: CAH73374.1.
AL356953, AC099336, AL589762 Genomic DNA. Translation: CAI15934.1.
AL356953, AC099336, AL589762 Genomic DNA. Translation: CAI15935.1.
CH471067 Genomic DNA. Translation: EAW91418.1.
BC034430 mRNA. Translation: AAH34430.1.
BC110907 mRNA. Translation: AAI10908.1.
BC144699 mRNA. Translation: AAI44700.1.
CCDSiCCDS1426.1. [O60237-1]
CCDS44294.1. [O60237-4]
CCDS44295.1. [O60237-3]
CCDS53458.1. [O60237-5]
CCDS53459.1. [O60237-2]
RefSeqiNP_001161329.1. NM_001167857.1. [O60237-5]
NP_001161330.1. NM_001167858.1. [O60237-2]
NP_001184060.1. NM_001197131.1.
NP_002472.2. NM_002481.3. [O60237-1]
NP_115286.1. NM_032103.2. [O60237-4]
NP_115287.1. NM_032104.2. [O60237-3]
XP_016856841.1. XM_017001352.1. [O60237-3]
XP_016856842.1. XM_017001353.1. [O60237-4]
XP_016856843.1. XM_017001354.1. [O60237-3]
UniGeneiHs.444403.
Hs.677346.

Genome annotation databases

EnsembliENST00000290419; ENSP00000484005; ENSG00000077157. [O60237-3]
ENST00000356764; ENSP00000349206; ENSG00000077157. [O60237-2]
ENST00000480184; ENSP00000417159; ENSG00000077157. [O60237-5]
ENST00000491336; ENSP00000480852; ENSG00000077157. [O60237-4]
ENST00000608999; ENSP00000476755; ENSG00000077157. [O60237-1]
GeneIDi4660.
KEGGihsa:4660.
UCSCiuc001gxy.4. human. [O60237-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003062 mRNA. Translation: BAA28376.1.
AF324888 mRNA. Translation: AAK00337.1.
AF324892
, AF324889, AF324890, AF324891 Genomic DNA. Translation: AAK00336.1.
AB050641 mRNA. Translation: BAB17610.1.
AB050642 mRNA. Translation: BAB17611.1.
AL589762, AC099336, AL356953 Genomic DNA. Translation: CAH73375.1.
AL589762, AC099336, AL356953 Genomic DNA. Translation: CAH73374.1.
AL356953, AC099336, AL589762 Genomic DNA. Translation: CAI15934.1.
AL356953, AC099336, AL589762 Genomic DNA. Translation: CAI15935.1.
CH471067 Genomic DNA. Translation: EAW91418.1.
BC034430 mRNA. Translation: AAH34430.1.
BC110907 mRNA. Translation: AAI10908.1.
BC144699 mRNA. Translation: AAI44700.1.
CCDSiCCDS1426.1. [O60237-1]
CCDS44294.1. [O60237-4]
CCDS44295.1. [O60237-3]
CCDS53458.1. [O60237-5]
CCDS53459.1. [O60237-2]
RefSeqiNP_001161329.1. NM_001167857.1. [O60237-5]
NP_001161330.1. NM_001167858.1. [O60237-2]
NP_001184060.1. NM_001197131.1.
NP_002472.2. NM_002481.3. [O60237-1]
NP_115286.1. NM_032103.2. [O60237-4]
NP_115287.1. NM_032104.2. [O60237-3]
XP_016856841.1. XM_017001352.1. [O60237-3]
XP_016856842.1. XM_017001353.1. [O60237-4]
XP_016856843.1. XM_017001354.1. [O60237-3]
UniGeneiHs.444403.
Hs.677346.

3D structure databases

ProteinModelPortaliO60237.
SMRiO60237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110743. 24 interactors.
IntActiO60237. 16 interactors.
STRINGi9606.ENSP00000384496.

PTM databases

iPTMnetiO60237.
PhosphoSitePlusiO60237.

Polymorphism and mutation databases

BioMutaiPPP1R12B.

2D gel databases

UCD-2DPAGEO60237.

Proteomic databases

MaxQBiO60237.
PaxDbiO60237.
PeptideAtlasiO60237.
PRIDEiO60237.

Protocols and materials databases

DNASUi4660.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290419; ENSP00000484005; ENSG00000077157. [O60237-3]
ENST00000356764; ENSP00000349206; ENSG00000077157. [O60237-2]
ENST00000480184; ENSP00000417159; ENSG00000077157. [O60237-5]
ENST00000491336; ENSP00000480852; ENSG00000077157. [O60237-4]
ENST00000608999; ENSP00000476755; ENSG00000077157. [O60237-1]
GeneIDi4660.
KEGGihsa:4660.
UCSCiuc001gxy.4. human. [O60237-1]

Organism-specific databases

CTDi4660.
DisGeNETi4660.
GeneCardsiPPP1R12B.
HGNCiHGNC:7619. PPP1R12B.
HPAiHPA024171.
HPA024640.
MIMi603768. gene.
neXtProtiNX_O60237.
OpenTargetsiENSG00000077157.
PharmGKBiPA33618.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000290648.
HOVERGENiHBG052561.
InParanoidiO60237.
KOiK12329.
OMAiSTEKHEP.
OrthoDBiEOG091G0UPK.
PhylomeDBiO60237.
TreeFamiTF105543.

Enzyme and pathway databases

BRENDAi3.1.3.53. 2681.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5627117. RHO GTPases Activate ROCKs.
R-HSA-5627123. RHO GTPases activate PAKs.
SIGNORiO60237.

Miscellaneous databases

ChiTaRSiPPP1R12B. human.
GeneWikiiPPP1R12B.
GenomeRNAii4660.
PROiO60237.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000077157.
CleanExiHS_PPP1R12B.
ExpressionAtlasiO60237. baseline and differential.
GenevisibleiO60237. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. MYPT1/MYPT2_chordates.
IPR031775. PRKG1_interact.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF15898. PRKG1_interact. 1 hit.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYPT2_HUMAN
AccessioniPrimary (citable) accession number: O60237
Secondary accession number(s): A8MYF5
, B7ZMN6, Q2TAI8, Q5T506, Q5VUK2, Q8N179, Q9HCB7, Q9HCB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 28, 2006
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.