O60235 (TM11D_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protease serine 11D
EC=3.4.21.-

Alternative name(s):
Airway trypsin-like protease

Cleaved into the following 2 chains:

1. Transmembrane protease serine 11D non-catalytic chain
2. Transmembrane protease serine 11D catalytic chain

Gene names

Name:	TMPRSS11D
Synonyms:	HAT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions.
Catalytic activity	Preferentially cleaves the C-terminal side of arginine residues at the P1 position of certain peptides, cleaving Boc-Phe-Ser-Arg-4-methylcoumaryl-7-amide most efficiently and having an optimum pH of 8.6 with this substrate.
Enzyme regulation	Strongly inhibited by diisopropyl fluorophosphate, leupeptin, antipain, aprotinin, and soybean trypsin inhibitor, but hardly inhibited by secretory leukocyte protease inhibitor at 10 microM.
Subunit structure	Monomer.
Subcellular location	Cell membrane; Single-pass type II membrane protein. Note: Activated by cleavage and secreted. Transmembrane protease serine 11D catalytic chain: Secreted. Note: Activated by cleavage and secreted.
Tissue specificity	Located in the cells of the submucosal serous glands of the bronchi and trachea.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 1 SEA domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW respiratory gaseous exchange Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular region Traceable author statement Ref.1. Source: ProtInc integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	peptidase activity Traceable author statement Ref.1. Source: ProtInc serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Transmembrane protease serine 11D non-catalytic chain		PRO_0000027885
	Chain	187 – 418	232	Transmembrane protease serine 11D catalytic chain		PRO_0000027886
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	42 – 418	377	Extracellular Potential
	Domain	44 – 164	121	SEA
	Domain	187 – 417	231	Peptidase S1
Sites
	Active site	227	1	Charge relay system By similarity
	Active site	272	1	Charge relay system By similarity
	Active site	368	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Disulfide bond	173 ↔ 292		Interchain (between non-catalytic and catalytic chains) By similarity
	Disulfide bond	212 ↔ 228		By similarity
	Disulfide bond	337 ↔ 353		By similarity
	Disulfide bond	364 ↔ 393		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O60235 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F4BC1DB020CFBBD0	FASTA	418	46,263
	10 20 30 40 50 60 MYRPARVTST SRFLNPYVVC FIVVAGVVIL AVTIALLVYF LAFDQKSYFY RSSFQLLNVE 70 80 90 100 110 120 YNSQLNSPAT QEYRTLSGRI ESLITKTFKE SNLRNQFIRA HVAKLRQDGS GVRADVVMKF 130 140 150 160 170 180 QFTRNNNGAS MKSRIESVLR QMLNNSGNLE INPSTEITSL TDQAAANWLI NECGAGPDLI 190 200 210 220 230 240 TLSEQRILGG TEAEEGSWPW QVSLRLNNAH HCGGSLINNM WILTAAHCFR SNSNPRDWIA 250 260 270 280 290 300 TSGISTTFPK LRMRVRNILI HNNYKSATHE NDIALVRLEN SVTFTKDIHS VCLPAATQNI 310 320 330 340 350 360 PPGSTAYVTG WGAQEYAGHT VPELRQGQVR IISNDVCNAP HSYNGAILSG MLCAGVPQGG 370 380 390 400 410 VDACQGDSGG PLVQEDSRRL WFIVGIVSWG DQCGLPDKPG VYTRVTAYLD WIRQQTGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the cDNA for human airway trypsin-like protease." Yamaoka K., Masuda K., Ogawa H., Takagi K., Umemoto N., Yasuoka S. J. Biol. Chem. 273:11895-11901(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Purification, characterization, and localization of a novel trypsin-like protease found in the human airway." Yasuoka S., Ohnishi T., Kawano S., Tsuchihashi S., Ogawara M., Masuda K., Yamaoka K., Takahashi M., Sano T. Am. J. Respir. Cell Mol. Biol. 16:300-308(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 187-206, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB002134 mRNA. Translation: BAA28691.1. BC125195 mRNA. Translation: AAI25196.1. BC125196 mRNA. Translation: AAI25197.1.
IPI	IPI00003542.
RefSeq	NP_004253.1. NM_004262.2.
UniGene	Hs.132195.
3D structure databases
ProteinModelPortal	O60235.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000283916.
Protein family/group databases
MEROPS	S01.047.
Proteomic databases
PaxDb	O60235.
PRIDE	O60235.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283916; ENSP00000283916; ENSG00000153802.
GeneID	9407.
KEGG	hsa:9407.
UCSC	uc003hdq.3. human.
Organism-specific databases
CTD	9407.
GeneCards	GC04M068686.
HGNC	HGNC:24059. TMPRSS11D.
HPA	HPA052834.
MIM	605369. gene.
neXtProt	NX_O60235.
PharmGKB	PA142670728.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251823.
HOVERGEN	HBG013304.
InParanoid	O60235.
KO	K09641.
OMA	DVVMKFR.
OrthoDB	EOG4BZN2M.
PhylomeDB	O60235.
Gene expression databases
ArrayExpress	O60235.
Bgee	O60235.
CleanEx	HS_TMPRSS11D.
Genevestigator	O60235.
GermOnline	ENSG00000153802. Homo sapiens.
Family and domain databases
InterPro	IPR017329. Pept_S1A_HAT/DESC1. IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR000082. SEA. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF01390. SEA. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PIRSF	PIRSF037941. TMPRSS11ABCDE. 1 hit.
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00200. SEA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50024. SEA. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O60235.
ChEMBL	CHEMBL1795138.
GenomeRNAi	9407.
NextBio	35242.
SOURCE	Search...

Entry information

Entry name

TM11D_HUMAN

Accession

Primary (citable) accession number: O60235
Secondary accession number(s): Q08AF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.