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Protein

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

Gene

DHX16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase involved in pre-mRNA splicing.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 429ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: Reactome
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB
  • RNA helicase activity Source: ProtInc

GO - Biological processi

  • mRNA splicing, via spliceosome Source: GO_Central
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137333-MONOMER.
BRENDAi3.6.4.13. 2681.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase #3
DEAH-box protein 16
Gene namesi
Name:DHX16
Synonyms:DBP2, DDX16, KIAA0577
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:2739. DHX16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
  • spliceosomal complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8449.
OpenTargetsiENSG00000204560.
ENSG00000226171.
ENSG00000231377.
ENSG00000233049.
ENSG00000233418.
ENSG00000233561.
PharmGKBiPA27205.

Polymorphism and mutation databases

BioMutaiDHX16.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551511 – 1041Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16Add BLAST1041

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103PhosphoserineCombined sources1
Modified residuei106PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Modified residuei712PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60231.
PaxDbiO60231.
PeptideAtlasiO60231.
PRIDEiO60231.

PTM databases

iPTMnetiO60231.
PhosphoSitePlusiO60231.

Expressioni

Gene expression databases

BgeeiENSG00000204560.
CleanExiHS_DHX16.
ExpressionAtlasiO60231. baseline and differential.
GenevisibleiO60231. HS.

Organism-specific databases

HPAiHPA051455.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DHX38Q926202EBI-311446,EBI-1043041
GPKOWQ929172EBI-311446,EBI-746309
SF3B2Q134352EBI-311446,EBI-749111
XAB2Q9HCS72EBI-311446,EBI-295232

Protein-protein interaction databases

BioGridi114027. 53 interactors.
IntActiO60231. 21 interactors.
MINTiMINT-5003862.
STRINGi9606.ENSP00000365625.

Structurei

3D structure databases

ProteinModelPortaliO60231.
SMRiO60231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini409 – 573Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST165
Domaini598 – 771Helicase C-terminalPROSITE-ProRule annotationAdd BLAST174

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi520 – 523DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO60231.
KOiK12813.
OMAiTVNVRKA.
OrthoDBiEOG091G030Q.
PhylomeDBiO60231.
TreeFamiTF313473.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPAGLERW VQDELHSVLG LSERHVAQFL IGTAQRCTSA EEFVQRLRDT
60 70 80 90 100
DTLDLSGPAR DFALRLWNKV PRKAVVEKPA RAAEREARAL LEKNRSYRLL
110 120 130 140 150
EDSEESSEET VSRAGSSLQK KRKKRKHLRK KREEEEEEEA SEKGKKKTGG
160 170 180 190 200
SKQQTEKPES EDEWERTERE RLQDLEERDA FAERVRQRDK DRTRNVLERS
210 220 230 240 250
DKKAYEEAQK RLKMAEEDRK AMVPELRKKS RREYLAKRER EKLEDLEAEL
260 270 280 290 300
ADEEFLFGDV ELSRHERQEL KYKRRVRDLA REYRAAGEQE KLEATNRYHM
310 320 330 340 350
PKETRGQPAR AVDLVEEESG APGEEQRRWE EARLGAASLK FGARDAASQE
360 370 380 390 400
PKYQLVLEEE ETIEFVRATQ LQGDEEPSAP PTSTQAQQKE SIQAVRRSLP
410 420 430 440 450
VFPFREELLA AIANHQVLII EGETGSGKTT QIPQYLFEEG YTNKGMKIAC
460 470 480 490 500
TQPRRVAAMS VAARVAREMG VKLGNEVGYS IRFEDCTSER TVLRYMTDGM
510 520 530 540 550
LLREFLSEPD LASYSVVMVD EAHERTLHTD ILFGLIKDVA RFRPELKVLV
560 570 580 590 600
ASATMDTARF STFFDDAPVF RIPGRRFPVD IFYTKAPEAD YLEACVVSVL
610 620 630 640 650
QIHVTQPPGD ILVFLTGQEE IEAACEMLQD RCRRLGSKIR ELLVLPIYAN
660 670 680 690 700
LPSDMQARIF QPTPPGARKV VVATNIAETS LTIEGIIYVL DPGFCKQKSY
710 720 730 740 750
NPRTGMESLT VTPCSKASAN QRAGRAGRVA AGKCFRLYTA WAYQHELEET
760 770 780 790 800
TVPEIQRTSL GNVVLLLKSL GIHDLMHFDF LDPPPYETLL LALEQLYALG
810 820 830 840 850
ALNHLGELTT SGRKMAELPV DPMLSKMILA SEKYSCSEEI LTVAAMLSVN
860 870 880 890 900
NSIFYRPKDK VVHADNARVN FFLPGGDHLV LLNVYTQWAE SGYSSQWCYE
910 920 930 940 950
NFVQFRSMRR ARDVREQLEG LLERVEVGLS SCQGDYIRVR KAITAGYFYH
960 970 980 990 1000
TARLTRSGYR TVKQQQTVFI HPNSSLFEQQ PRWLLYHELV LTTKEFMRQV
1010 1020 1030 1040
LEIESSWLLE VAPHYYKAKE LEDPHAKKMP KKIGKTREEL G
Length:1,041
Mass (Da):119,264
Last modified:November 28, 2002 - v2
Checksum:i5CAC4DBEF21A4E6D
GO

Sequence cautioni

The sequence BAA25503 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223V → I in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti631R → P in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti681L → P in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti792A → T in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti899Y → C in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti1032K → KK in AAH08825 (PubMed:15489334).Curated1
Sequence conflicti1032K → KK in AAH09392 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057236352K → E.Corresponds to variant rs17189239dbSNPEnsembl.1
Natural variantiVAR_057237502L → F.Corresponds to variant rs17189232dbSNPEnsembl.1
Natural variantiVAR_057238566D → G.1 PublicationCorresponds to variant rs9262138dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001601 mRNA. Translation: BAA25908.1.
BA000025 Genomic DNA. Translation: BAB63323.1.
AB011149 mRNA. Translation: BAA25503.2. Different initiation.
AL845353 Genomic DNA. Translation: CAI41883.1.
BC008825 mRNA. Translation: AAH08825.1.
BC009392 mRNA. Translation: AAH09392.1.
CCDSiCCDS4685.1.
RefSeqiNP_001157711.1. NM_001164239.1.
NP_003578.2. NM_003587.4.
UniGeneiHs.485060.

Genome annotation databases

EnsembliENST00000376442; ENSP00000365625; ENSG00000204560.
ENST00000383577; ENSP00000373071; ENSG00000206486.
ENST00000417308; ENSP00000390938; ENSG00000233561.
ENST00000421095; ENSP00000396193; ENSG00000226171.
ENST00000424672; ENSP00000389862; ENSG00000233418.
ENST00000451456; ENSP00000408956; ENSG00000233049.
ENST00000458094; ENSP00000393958; ENSG00000231377.
GeneIDi8449.
KEGGihsa:8449.
UCSCiuc011ijv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001601 mRNA. Translation: BAA25908.1.
BA000025 Genomic DNA. Translation: BAB63323.1.
AB011149 mRNA. Translation: BAA25503.2. Different initiation.
AL845353 Genomic DNA. Translation: CAI41883.1.
BC008825 mRNA. Translation: AAH08825.1.
BC009392 mRNA. Translation: AAH09392.1.
CCDSiCCDS4685.1.
RefSeqiNP_001157711.1. NM_001164239.1.
NP_003578.2. NM_003587.4.
UniGeneiHs.485060.

3D structure databases

ProteinModelPortaliO60231.
SMRiO60231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114027. 53 interactors.
IntActiO60231. 21 interactors.
MINTiMINT-5003862.
STRINGi9606.ENSP00000365625.

PTM databases

iPTMnetiO60231.
PhosphoSitePlusiO60231.

Polymorphism and mutation databases

BioMutaiDHX16.

Proteomic databases

EPDiO60231.
PaxDbiO60231.
PeptideAtlasiO60231.
PRIDEiO60231.

Protocols and materials databases

DNASUi8449.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376442; ENSP00000365625; ENSG00000204560.
ENST00000383577; ENSP00000373071; ENSG00000206486.
ENST00000417308; ENSP00000390938; ENSG00000233561.
ENST00000421095; ENSP00000396193; ENSG00000226171.
ENST00000424672; ENSP00000389862; ENSG00000233418.
ENST00000451456; ENSP00000408956; ENSG00000233049.
ENST00000458094; ENSP00000393958; ENSG00000231377.
GeneIDi8449.
KEGGihsa:8449.
UCSCiuc011ijv.3. human.

Organism-specific databases

CTDi8449.
DisGeNETi8449.
GeneCardsiDHX16.
HGNCiHGNC:2739. DHX16.
HPAiHPA051455.
MIMi603405. gene.
neXtProtiNX_O60231.
OpenTargetsiENSG00000204560.
ENSG00000226171.
ENSG00000231377.
ENSG00000233049.
ENSG00000233418.
ENSG00000233561.
PharmGKBiPA27205.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO60231.
KOiK12813.
OMAiTVNVRKA.
OrthoDBiEOG091G030Q.
PhylomeDBiO60231.
TreeFamiTF313473.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137333-MONOMER.
BRENDAi3.6.4.13. 2681.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

GeneWikiiDHX16.
GenomeRNAii8449.
PROiO60231.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204560.
CleanExiHS_DHX16.
ExpressionAtlasiO60231. baseline and differential.
GenevisibleiO60231. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX16_HUMAN
AccessioniPrimary (citable) accession number: O60231
Secondary accession number(s): O60322
, Q5JP45, Q969X7, Q96QC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 28, 2002
Last modified: November 2, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.