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Protein

Aldo-keto reductase family 1 member B10

Gene

AKR1B10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.1 Publication

Enzyme regulationi

Inhibited by tolrestat.1 Publication

Kineticsi

  1. KM=600 µM for D,L-glyceraldehyde1 Publication
  2. KM=0.6 µM for all-trans-retinaldehyde1 Publication
  3. KM=0.7 µM for 9-cis-retinaldehyde1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei44NADP1 Publication1
    Active sitei49Proton donor1 Publication1
    Sitei78Lowers pKa of active site TyrBy similarity1
    Binding sitei111Substrate1 Publication1
    Binding sitei184NADP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi20 – 22NADP1 Publication3
    Nucleotide bindingi160 – 161NADP1 Publication2
    Nucleotide bindingi210 – 217NADP1 Publication8
    Nucleotide bindingi261 – 273NADP1 PublicationAdd BLAST13

    GO - Molecular functioni

    • aldo-keto reductase (NADP) activity Source: UniProtKB
    • geranylgeranyl reductase activity Source: UniProtKB
    • indanol dehydrogenase activity Source: UniProtKB
    • retinal dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    • cellular aldehyde metabolic process Source: ProtInc
    • daunorubicin metabolic process Source: UniProtKB
    • digestion Source: ProtInc
    • doxorubicin metabolic process Source: UniProtKB
    • farnesol catabolic process Source: UniProtKB
    • retinoid metabolic process Source: Reactome
    • steroid metabolic process Source: ProtInc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciZFISH:HS02925-MONOMER.
    BRENDAi1.1.1.21. 2681.
    ReactomeiR-HSA-975634. Retinoid metabolism and transport.
    SABIO-RKO60218.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldo-keto reductase family 1 member B10 (EC:1.1.1.-)
    Alternative name(s):
    ARL-1
    Aldose reductase-like
    Aldose reductase-related protein
    Short name:
    ARP
    Short name:
    hARP
    Small intestine reductase
    Short name:
    SI reductase
    Gene namesi
    Name:AKR1B10
    Synonyms:AKR1B11
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:382. AKR1B10.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: Reactome
    • extracellular exosome Source: UniProtKB
    • lysosome Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi125K → L: Increased affinity and reduced catalytic activity towards all-trans-retinaldehyde. 1 Publication1
    Mutagenesisi301V → L: Reduced catalytic activity towards all-trans-retinaldehyde. 1 Publication1

    Organism-specific databases

    DisGeNETi57016.
    OpenTargetsiENSG00000198074.
    PharmGKBiPA24676.

    Chemistry databases

    ChEMBLiCHEMBL5983.

    Polymorphism and mutation databases

    BioMutaiAKR1B10.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001246321 – 316Aldo-keto reductase family 1 member B10Add BLAST316

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei125N6-acetyllysineCombined sources1
    Modified residuei263N6-acetyllysineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO60218.
    PaxDbiO60218.
    PeptideAtlasiO60218.
    PRIDEiO60218.

    2D gel databases

    DOSAC-COBS-2DPAGEO60218.

    PTM databases

    iPTMnetiO60218.
    PhosphoSitePlusiO60218.

    Expressioni

    Tissue specificityi

    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

    Gene expression databases

    BgeeiENSG00000198074.
    CleanExiHS_AKR1B10.
    GenevisibleiO60218. HS.

    Organism-specific databases

    HPAiCAB070163.
    HPA020280.
    HPA073633.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACACAQ130854EBI-1572139,EBI-717681
    TERF2IPQ9NYB02EBI-1572139,EBI-750109

    Protein-protein interaction databases

    BioGridi121325. 18 interactors.
    IntActiO60218. 6 interactors.
    STRINGi9606.ENSP00000352584.

    Chemistry databases

    BindingDBiO60218.

    Structurei

    Secondary structure

    1316
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 6Combined sources3
    Beta strandi12 – 19Combined sources8
    Turni25 – 27Combined sources3
    Helixi28 – 37Combined sources10
    Beta strandi42 – 44Combined sources3
    Helixi47 – 49Combined sources3
    Helixi52 – 64Combined sources13
    Helixi70 – 72Combined sources3
    Beta strandi74 – 79Combined sources6
    Helixi81 – 83Combined sources3
    Helixi86 – 100Combined sources15
    Beta strandi105 – 111Combined sources7
    Beta strandi118 – 120Combined sources3
    Beta strandi127 – 131Combined sources5
    Helixi138 – 150Combined sources13
    Beta strandi153 – 161Combined sources9
    Helixi164 – 171Combined sources8
    Beta strandi181 – 186Combined sources6
    Helixi194 – 202Combined sources9
    Beta strandi206 – 211Combined sources6
    Helixi228 – 230Combined sources3
    Helixi232 – 240Combined sources9
    Helixi245 – 254Combined sources10
    Turni255 – 257Combined sources3
    Helixi267 – 274Combined sources8
    Helixi283 – 290Combined sources8
    Helixi302 – 304Combined sources3
    Beta strandi305 – 307Combined sources3
    Helixi311 – 313Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1ZUAX-ray1.25X1-316[»]
    4GA8X-ray1.94A1-316[»]
    4GABX-ray1.60A1-316[»]
    4GQ0X-ray2.10A1-316[»]
    4GQGX-ray1.92A1-316[»]
    4I5XX-ray2.10A1-316[»]
    4ICCX-ray1.75X1-316[»]
    4JIHX-ray2.30A1-316[»]
    4JIIX-ray2.20X1-316[»]
    4WEVX-ray1.45X1-316[»]
    4XZLX-ray1.70X1-316[»]
    4XZMX-ray1.75X1-316[»]
    4XZNX-ray1.70X1-316[»]
    5LIKX-ray2.05X1-316[»]
    5LIUX-ray1.75X1-316[»]
    5LIWX-ray1.75X1-316[»]
    5LIXX-ray1.95X1-316[»]
    5LIYX-ray2.05X1-316[»]
    ProteinModelPortaliO60218.
    SMRiO60218.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO60218.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiKOG1577. Eukaryota.
    COG0656. LUCA.
    GeneTreeiENSGT00760000119041.
    HOGENOMiHOG000250272.
    HOVERGENiHBG000020.
    InParanoidiO60218.
    KOiK00011.
    OMAiVSNFSHF.
    OrthoDBiEOG091G0D69.
    PhylomeDBiO60218.
    TreeFamiTF106492.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 2 hits.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60218-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ
    60 70 80 90 100
    NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL
    110 120 130 140 150
    KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD
    160 170 180 190 200
    EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC
    210 220 230 240 250
    HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI
    260 270 280 290 300
    RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN
    310
    VLQSSHLEDY PFNAEY
    Length:316
    Mass (Da):36,020
    Last modified:February 8, 2011 - v2
    Checksum:i0C2FC0E798955A33
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti76V → A in AAO13380 (Ref. 3) Curated1
    Sequence conflicti100L → P in AAO13380 (Ref. 3) Curated1
    Sequence conflicti288T → I in AAO13380 (Ref. 3) Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_02007787P → S.Corresponds to variant rs2303312dbSNPEnsembl.1
    Natural variantiVAR_020078286M → T.Corresponds to variant rs3735042dbSNPEnsembl.1
    Natural variantiVAR_013287313N → D.Combined sources6 PublicationsCorresponds to variant rs4728329dbSNPEnsembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U37100 mRNA. Translation: AAC17469.1.
    AF052577 mRNA. Translation: AAC36465.1.
    AF524864 mRNA. Translation: AAO13380.1.
    BT006794 mRNA. Translation: AAP35440.1.
    CR541801 mRNA. Translation: CAG46600.1.
    AC078847 Genomic DNA. No translation available.
    CH236950 Genomic DNA. Translation: EAL24069.1.
    CH471070 Genomic DNA. Translation: EAW83816.1.
    BC008837 mRNA. Translation: AAH08837.1.
    AF044961 mRNA. Translation: AAC15671.1.
    CCDSiCCDS5832.1.
    RefSeqiNP_064695.3. NM_020299.4.
    UniGeneiHs.116724.

    Genome annotation databases

    EnsembliENST00000359579; ENSP00000352584; ENSG00000198074.
    GeneIDi57016.
    KEGGihsa:57016.
    UCSCiuc003vrr.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U37100 mRNA. Translation: AAC17469.1.
    AF052577 mRNA. Translation: AAC36465.1.
    AF524864 mRNA. Translation: AAO13380.1.
    BT006794 mRNA. Translation: AAP35440.1.
    CR541801 mRNA. Translation: CAG46600.1.
    AC078847 Genomic DNA. No translation available.
    CH236950 Genomic DNA. Translation: EAL24069.1.
    CH471070 Genomic DNA. Translation: EAW83816.1.
    BC008837 mRNA. Translation: AAH08837.1.
    AF044961 mRNA. Translation: AAC15671.1.
    CCDSiCCDS5832.1.
    RefSeqiNP_064695.3. NM_020299.4.
    UniGeneiHs.116724.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1ZUAX-ray1.25X1-316[»]
    4GA8X-ray1.94A1-316[»]
    4GABX-ray1.60A1-316[»]
    4GQ0X-ray2.10A1-316[»]
    4GQGX-ray1.92A1-316[»]
    4I5XX-ray2.10A1-316[»]
    4ICCX-ray1.75X1-316[»]
    4JIHX-ray2.30A1-316[»]
    4JIIX-ray2.20X1-316[»]
    4WEVX-ray1.45X1-316[»]
    4XZLX-ray1.70X1-316[»]
    4XZMX-ray1.75X1-316[»]
    4XZNX-ray1.70X1-316[»]
    5LIKX-ray2.05X1-316[»]
    5LIUX-ray1.75X1-316[»]
    5LIWX-ray1.75X1-316[»]
    5LIXX-ray1.95X1-316[»]
    5LIYX-ray2.05X1-316[»]
    ProteinModelPortaliO60218.
    SMRiO60218.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi121325. 18 interactors.
    IntActiO60218. 6 interactors.
    STRINGi9606.ENSP00000352584.

    Chemistry databases

    BindingDBiO60218.
    ChEMBLiCHEMBL5983.

    PTM databases

    iPTMnetiO60218.
    PhosphoSitePlusiO60218.

    Polymorphism and mutation databases

    BioMutaiAKR1B10.

    2D gel databases

    DOSAC-COBS-2DPAGEO60218.

    Proteomic databases

    MaxQBiO60218.
    PaxDbiO60218.
    PeptideAtlasiO60218.
    PRIDEiO60218.

    Protocols and materials databases

    DNASUi57016.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000359579; ENSP00000352584; ENSG00000198074.
    GeneIDi57016.
    KEGGihsa:57016.
    UCSCiuc003vrr.4. human.

    Organism-specific databases

    CTDi57016.
    DisGeNETi57016.
    GeneCardsiAKR1B10.
    H-InvDBHIX0007102.
    HIX0033661.
    HGNCiHGNC:382. AKR1B10.
    HPAiCAB070163.
    HPA020280.
    HPA073633.
    MIMi604707. gene.
    neXtProtiNX_O60218.
    OpenTargetsiENSG00000198074.
    PharmGKBiPA24676.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1577. Eukaryota.
    COG0656. LUCA.
    GeneTreeiENSGT00760000119041.
    HOGENOMiHOG000250272.
    HOVERGENiHBG000020.
    InParanoidiO60218.
    KOiK00011.
    OMAiVSNFSHF.
    OrthoDBiEOG091G0D69.
    PhylomeDBiO60218.
    TreeFamiTF106492.

    Enzyme and pathway databases

    BioCyciZFISH:HS02925-MONOMER.
    BRENDAi1.1.1.21. 2681.
    ReactomeiR-HSA-975634. Retinoid metabolism and transport.
    SABIO-RKO60218.

    Miscellaneous databases

    EvolutionaryTraceiO60218.
    GeneWikiiAKR1B10.
    GenomeRNAii57016.
    PROiO60218.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000198074.
    CleanExiHS_AKR1B10.
    GenevisibleiO60218. HS.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 2 hits.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAK1BA_HUMAN
    AccessioniPrimary (citable) accession number: O60218
    Secondary accession number(s): A4D1P1
    , O75890, Q6FHF3, Q8IWZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: February 8, 2011
    Last modified: November 30, 2016
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.