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Protein

Probable aspartokinase

Gene

SPBC19F5.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable aspartokinase (SPBC19F5.04)
  2. Probable aspartate-semialdehyde dehydrogenase (SPCC1827.06c)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable aspartokinase (SPBC19F5.04)
  2. Probable aspartate-semialdehyde dehydrogenase (SPCC1827.06c)
  3. Probable homoserine dehydrogenase (SPBC776.03)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable aspartokinase (SPBC19F5.04)
  2. Probable aspartate-semialdehyde dehydrogenase (SPCC1827.06c)
  3. Probable homoserine dehydrogenase (SPBC776.03)
  4. Probable homoserine kinase (SPBC4C3.03)
  5. Threonine synthase (thrc)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aspartokinase (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase
Gene namesi
ORF Names:SPBC19F5.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19F5.04.
PomBaseiSPBC19F5.04.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666891 – 519Probable aspartokinaseAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei326Phosphoserine1 Publication1
Modified residuei328Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60163.
PRIDEiO60163.

PTM databases

iPTMnetiO60163.
SwissPalmiO60163.

Interactioni

Protein-protein interaction databases

BioGridi277128. 1 interactor.
MINTiMINT-4677697.

Structurei

3D structure databases

ProteinModelPortaliO60163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini363 – 435ACT 1PROSITE-ProRule annotationAdd BLAST73
Domaini436 – 510ACT 2PROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the aspartokinase family.Curated
Contains 2 ACT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000293094.
InParanoidiO60163.
KOiK00928.
OMAiKFVITSQ.
OrthoDBiEOG092C1PG8.
PhylomeDBiO60163.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIAKNIEND PSKGWVVQKF GGTSVGKFPI KIAVDVAKEY LSTKRVALVC
60 70 80 90 100
SARSTDTKAE GTTTRLIRAT EAALRPAVGS VHDLVRIIET DHVQAARDFI
110 120 130 140 150
QDVGIQDELI DAFHADCVEL EQYLNAIRVL SEVSPRTRDL VIGMGERLSC
160 170 180 190 200
RFMAAVLKDQ GIDSEFIDMS HIIDEQREWR NLDASFYAYL ASQLASKVTA
210 220 230 240 250
VGNKVPVVTG FFGMVPGGLL SQIGRGYTDF CAALLAVGLN ADELQIWKEV
260 270 280 290 300
DGIFTADPRK VPTARLLPLI TPEEAAELTY YGSEVIHPFT MSQVVHARIP
310 320 330 340 350
IRIKNVGNPR GKGTVIFPDT ISRHGSATPP HPPKIMPDDI SASLANKGAT
360 370 380 390 400
AVTIKDTIMV INIQSNRKIS AHGFLASIFA ILDKYKLAVD LITTSEVHVS
410 420 430 440 450
MALYEESDDG NMHEAFVELR RLGTLDILHG LAILSLVGKH MRNTTGYAGR
460 470 480 490 500
MFCKLAEAQI NIEMISQGAS EINISCVIDE KMAVKALNVI HKELLEPLAL
510
HEVPSQASML VEKPWLYSA
Length:519
Mass (Da):56,813
Last modified:August 1, 1998 - v1
Checksum:i0B392EB4AE8ECFCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18652.1.
PIRiT39822.
RefSeqiNP_596542.1. NM_001022463.2.

Genome annotation databases

EnsemblFungiiSPBC19F5.04.1; SPBC19F5.04.1:pep; SPBC19F5.04.
GeneIDi2540602.
KEGGispo:SPBC19F5.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18652.1.
PIRiT39822.
RefSeqiNP_596542.1. NM_001022463.2.

3D structure databases

ProteinModelPortaliO60163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277128. 1 interactor.
MINTiMINT-4677697.

PTM databases

iPTMnetiO60163.
SwissPalmiO60163.

Proteomic databases

MaxQBiO60163.
PRIDEiO60163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC19F5.04.1; SPBC19F5.04.1:pep; SPBC19F5.04.
GeneIDi2540602.
KEGGispo:SPBC19F5.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC19F5.04.
PomBaseiSPBC19F5.04.

Phylogenomic databases

HOGENOMiHOG000293094.
InParanoidiO60163.
KOiK00928.
OMAiKFVITSQ.
OrthoDBiEOG092C1PG8.
PhylomeDBiO60163.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Miscellaneous databases

PROiO60163.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAK_SCHPO
AccessioniPrimary (citable) accession number: O60163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.