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Protein

tRNA wybutosine-synthesizing protein 4

Gene

ppm2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe).
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO2 = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66S-adenosyl-L-methionineBy similarity1
Binding sitei92S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei119S-adenosyl-L-methionineBy similarity1
Binding sitei193S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 4 (EC:2.1.1.290, EC:2.3.1.231)
Short name:
tRNA-yW synthesizing protein 4
Alternative name(s):
Leucine carboxyl methyltransferase 2
tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
Gene namesi
Name:ppm2
Synonyms:tyw4
ORF Names:SPBC19C7.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C7.08c.
PomBaseiSPBC19C7.08c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002261441 – 681tRNA wybutosine-synthesizing protein 4Add BLAST681

Proteomic databases

MaxQBiO60157.
PRIDEiO60157.

Interactioni

Protein-protein interaction databases

BioGridi277225. 8 interactors.
MINTiMINT-4677652.

Structurei

3D structure databases

ProteinModelPortaliO60157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 166S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

InParanoidiO60157.
KOiK15451.
OMAiYPETIEN.
OrthoDBiEOG092C0IJS.
PhylomeDBiO60157.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR011043. Gal_Oxase/kelch_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 1 hit.
SSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

O60157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKNQRKTK SKDREVRKTN DSSILSKASV EKCGYPGFTV GHSYYQPFIQ
60 70 80 90 100
KSPRRSPSVN RGYWTRCMAI RFAVYQFLKN KTGKRKAIVN LGAGYDPLAF
110 120 130 140 150
QLLSSHEYNT DDVVFYDVDY PETIENRVQM IRSDSFLSSI VLEDKEFDLD
160 170 180 190 200
GTEIHTKNYH SFGCNLNLLN QLESCLEKYG IDYCNDAILF ISEVAAVYMP
210 220 230 240 250
RQASEKLIRW MSKFPDAHSC FFEQIAPATF DHPFANVMVK HFKEWGTPLH
260 270 280 290 300
GLYAYPTIES LKSRWVKNGW EYVEILDVCT FWNFLMDSKL KHLCEMVEPF
310 320 330 340 350
DEWEEFYFFL QHYSIQHASS KLVGKYDLVE SPDPCMQYIR YVKSEIIFNN
360 370 380 390 400
SPLTLRNSIY SLKRTDLPAC LKELPSLRLP AVCDLDDSVI VQGGLSTAGR
410 420 430 440 450
SKDAYLISEK DDGSIMKITT DSLTSCMGQS VVSIDKKTCM FIGGRESPKK
460 470 480 490 500
ILSSCIYFAD GNWSDFPSLP YASHRASSVS IKHNGSSYVV LLAGKPFGGC
510 520 530 540 550
LIWSDSKRKW NTLKCKDPLF YSRWGACLHW SSKLKKGILC GGMNELNEPV
560 570 580 590 600
REVLEWEMVL RDDDHFEIVT RVLNFDVQVE ILLSRIGSKV VFFGDDSKPI
610 620 630 640 650
IVGGAAVFRT ILWEEESVCI NMNDYSVTGV CIEETEKRPV FTMFGISGMG
660 670 680
NHLQIFGGGC ICFSFGSCLN ENATFYKLVS A
Length:681
Mass (Da):77,553
Last modified:August 1, 1998 - v1
Checksum:i25070B74822D39F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19576.1.
PIRiT39814.
RefSeqiNP_596164.1. NM_001022084.2.

Genome annotation databases

EnsemblFungiiSPBC19C7.08c.1; SPBC19C7.08c.1:pep; SPBC19C7.08c.
GeneIDi2540701.
KEGGispo:SPBC19C7.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19576.1.
PIRiT39814.
RefSeqiNP_596164.1. NM_001022084.2.

3D structure databases

ProteinModelPortaliO60157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277225. 8 interactors.
MINTiMINT-4677652.

Proteomic databases

MaxQBiO60157.
PRIDEiO60157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC19C7.08c.1; SPBC19C7.08c.1:pep; SPBC19C7.08c.
GeneIDi2540701.
KEGGispo:SPBC19C7.08c.

Organism-specific databases

EuPathDBiFungiDB:SPBC19C7.08c.
PomBaseiSPBC19C7.08c.

Phylogenomic databases

InParanoidiO60157.
KOiK15451.
OMAiYPETIEN.
OrthoDBiEOG092C0IJS.
PhylomeDBiO60157.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

PROiO60157.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR011043. Gal_Oxase/kelch_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 1 hit.
SSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTYW4_SCHPO
AccessioniPrimary (citable) accession number: O60157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.