Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O60156

- SEN34_SCHPO

UniProt

O60156 - SEN34_SCHPO

Protein

Probable tRNA-splicing endonuclease subunit sen34

Gene

sen34

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei206 – 2061By similarity
    Active sitei214 – 2141By similarity
    Active sitei245 – 2451By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: PomBase

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. tRNA-type intron splice site recognition and cleavage Source: PomBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA-splicing endonuclease subunit sen34 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease sen34
    Gene namesi
    Name:sen34
    ORF Names:SPBC19C7.07c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC19C7.07c.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial outer membrane Source: PomBase
    2. tRNA-intron endonuclease complex Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Probable tRNA-splicing endonuclease subunit sen34PRO_0000109467Add
    BLAST

    Proteomic databases

    MaxQBiO60156.

    Interactioni

    Subunit structurei

    Heterotetramer composed of sen2, sen15, sen34 and sen54. Interacts directly with sen15 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi4896.SPBC19C7.07c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO60156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000175584.
    KOiK15323.
    OMAiHASHIVI.
    OrthoDBiEOG7N905X.
    PhylomeDBiO60156.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O60156-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDEKFPISY VHGKFLVFDV QAVEVFRKNY HILGTLVGTL PQLPQQNVFL    50
    GLPMELSKEE AFYLIEKGIS YIVDDTKVHK QLLENTTKDD VKQCLKKRQS 100
    LAYDQMIAAK KKENEKKIEI MKKLGRTLPL DPLNYDEHDS FDLSWIPVDT 150
    VTTRIAEKSS MNDDFHKEED VFENLDINRY LMFKSLVDTG FYLNPGLRFG 200
    CQFVAYPGDA LRYHSHYLVN SYKWDQEIPV LFLIGGGRLG TAVKKTWLIG 250
    GSNDRNINMN GEKSKEELLL LPVRHFSIEW AGFG 284
    Length:284
    Mass (Da):32,808
    Last modified:August 1, 1998 - v1
    Checksum:iF40AFEBE0C50F567
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA19575.1.
    PIRiT39813.
    RefSeqiNP_596163.1. NM_001022083.2.

    Genome annotation databases

    EnsemblFungiiSPBC19C7.07c.1; SPBC19C7.07c.1:pep; SPBC19C7.07c.
    GeneIDi2540591.
    KEGGispo:SPBC19C7.07c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA19575.1 .
    PIRi T39813.
    RefSeqi NP_596163.1. NM_001022083.2.

    3D structure databases

    ProteinModelPortali O60156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4896.SPBC19C7.07c-1.

    Proteomic databases

    MaxQBi O60156.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC19C7.07c.1 ; SPBC19C7.07c.1:pep ; SPBC19C7.07c .
    GeneIDi 2540591.
    KEGGi spo:SPBC19C7.07c.

    Organism-specific databases

    PomBasei SPBC19C7.07c.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000175584.
    KOi K15323.
    OMAi HASHIVI.
    OrthoDBi EOG7N905X.
    PhylomeDBi O60156.

    Miscellaneous databases

    NextBioi 20801716.
    PROi O60156.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiSEN34_SCHPO
    AccessioniPrimary (citable) accession number: O60156
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3