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Protein

Probable tRNA-splicing endonuclease subunit sen34

Gene

sen34

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei206By similarity1
Active sitei214By similarity1
Active sitei245By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit sen34 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen34
Gene namesi
Name:sen34
ORF Names:SPBC19C7.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C7.07c
PomBaseiSPBC19C7.07c sen34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094671 – 284Probable tRNA-splicing endonuclease subunit sen34Add BLAST284

Proteomic databases

MaxQBiO60156
PaxDbiO60156
PRIDEiO60156

Interactioni

Subunit structurei

Heterotetramer composed of sen2, sen15, sen34 and sen54. Interacts directly with sen15 (By similarity).By similarity

Protein-protein interaction databases

BioGridi277117, 1 interactor
STRINGi4896.SPBC19C7.07c.1

Structurei

3D structure databases

ProteinModelPortaliO60156
SMRiO60156
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000175584
InParanoidiO60156
KOiK15323
OMAiRTFSIEW
OrthoDBiEOG092C3X38
PhylomeDBiO60156

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016690 tRNA_splic_SEN34
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF017250 tRNA_splic_SEN34, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

O60156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDEKFPISY VHGKFLVFDV QAVEVFRKNY HILGTLVGTL PQLPQQNVFL
60 70 80 90 100
GLPMELSKEE AFYLIEKGIS YIVDDTKVHK QLLENTTKDD VKQCLKKRQS
110 120 130 140 150
LAYDQMIAAK KKENEKKIEI MKKLGRTLPL DPLNYDEHDS FDLSWIPVDT
160 170 180 190 200
VTTRIAEKSS MNDDFHKEED VFENLDINRY LMFKSLVDTG FYLNPGLRFG
210 220 230 240 250
CQFVAYPGDA LRYHSHYLVN SYKWDQEIPV LFLIGGGRLG TAVKKTWLIG
260 270 280
GSNDRNINMN GEKSKEELLL LPVRHFSIEW AGFG
Length:284
Mass (Da):32,808
Last modified:August 1, 1998 - v1
Checksum:iF40AFEBE0C50F567
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA19575.1
PIRiT39813
RefSeqiNP_596163.1, NM_001022083.2

Genome annotation databases

EnsemblFungiiSPBC19C7.07c.1; SPBC19C7.07c.1:pep; SPBC19C7.07c
GeneIDi2540591
KEGGispo:SPBC19C7.07c

Similar proteinsi

Entry informationi

Entry nameiSEN34_SCHPO
AccessioniPrimary (citable) accession number: O60156
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health