O60139 (UBP4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 4
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4

Gene names

Name:	ubp4
ORF Names:	SPBC18H10.08c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Acts late in the proteolytic pathway in conjunction with the 26S proteasome. Plays a role in avoiding DNA overreplication By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with sfp47. Ref.4
Subcellular location	Cytoplasm. Endosome Ref.2 Ref.4.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Endosome
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from direct assay Ref.4. Source: PomBase ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cell division site Inferred from direct assay Ref.4. Source: PomBase endosome Inferred from direct assay Ref.4. Source: PomBase transport vesicle Inferred from direct assay Ref.4. Source: PomBase
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay Ref.4. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Probable ubiquitin carboxyl-terminal hydrolase 4		PRO_0000080605
Sites
	Active site	236	1	Nucleophile By similarity
	Active site	530	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	338	1	Phosphoserine Ref.3
	Modified residue	343	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O60139 [UniParc]. Last modified April 18, 2012. Version 2. Checksum: 16801C84BB258B61	FASTA	593	67,747
	10 20 30 40 50 60 MSDDYFDRLF ELAFVYINED ETIQSCSFRG QRWLEEAQTL EQKNSLLKAY YYYLKALKLA 70 80 90 100 110 120 YEIPCRFEIS VKSTHYGEFK QFQKLAIQAV SKAFTIKSKL AVKHYLPVIQ ISDALSLSKK 130 140 150 160 170 180 SSLKVLFLNF YSQESSKGYV FSKHTIAIPI SCLQSMDSSK IYDFLKSAPF HPSMVICYSL 190 200 210 220 230 240 ERYFEDVSLA YKLYSMLRSL KLDPHFMELA NPKKVDSSLS YENYQPIGLT NLGNTCYMNC 250 260 270 280 290 300 VLQCLFACKD LTIPMLQGRG LLQNINTKNP LGTGGKITSA FFSLLQSVLL NHGQRSISPR 310 320 330 340 350 360 NFLEIVQSLN RDFSIDGQCD AQEFLNFFLD KLHEDLNSNA SRSPIAPLTE DQLSAREELP 370 380 390 400 410 420 LSHFSHIEWN LHLRSNKSIV VNNFVGQLCS RTQCMTCGRT STTFAPFTSL AIPIDDVSHV 430 440 450 460 470 480 VSLQECLLKF SAPELLQGHD GWHCPVCKVQ RSAKKVIMIS KLPEYLIIQI QRFKISVMGR 490 500 510 520 530 540 KKIDTPLGLS LQIPSKMLVP PSFQSGIGYI PSNYNLFAFI CHYGQLENGH YISDVLFNNE 550 560 570 580 590 WCHIDDSIVR TVGGITDLRE DFSSSYILFY KRSSLLEEFE DKCPKMTLKR NVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND SER-343, MASS SPECTROMETRY.
[4]	"A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity." Kouranti I., McLean J.R., Feoktistova A., Liang P., Johnson A.E., Roberts-Galbraith R.H., Gould K.L. PLoS Biol. 8:0-0(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SFP47, SUBCELLULAR LOCATION.
[5]	"Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability." Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L., Hagan I.M., Miller C.J. Genetics 187:1207-1217(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVISION OF GENE MODEL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA18405.2.
PIR	T39772.
RefSeq	NP_595732.2. NM_001021630.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC18H10.08c-1.
Protein family/group databases
MEROPS	C19.A58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC18H10.08c.1; SPBC18H10.08c.1:pep; SPBC18H10.08c.
GeneID	2540837.
KEGG	spo:SPBC18H10.08c.
Organism-specific databases
PomBase	SPBC18H10.08c.
Phylogenomic databases
eggNOG	COG5533.
KO	K11839.
OrthoDB	EOG4HX88F.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801954.

Entry information

Entry name

UBP4_SCHPO

Accession

Primary (citable) accession number: O60139

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	April 18, 2012
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program