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O60139

- UBP4_SCHPO

UniProt

O60139 - UBP4_SCHPO

Protein

Probable ubiquitin carboxyl-terminal hydrolase 4

Gene

ubp4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (18 Apr 2012)
      Previous versions | rss
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    Functioni

    Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Acts late in the proteolytic pathway in conjunction with the 26S proteasome. Plays a role in avoiding DNA overreplication By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei236 – 2361NucleophilePROSITE-ProRule annotation
    Active sitei530 – 5301Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: PomBase
    2. ubiquitin-specific protease activity Source: PomBase

    GO - Biological processi

    1. protein deubiquitination Source: PomBase
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.A58.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:ubp4
    ORF Names:SPBC18H10.08c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC18H10.08c.

    Subcellular locationi

    GO - Cellular componenti

    1. cell division site Source: PomBase
    2. cytoplasm Source: PomBase
    3. endosome Source: PomBase
    4. transport vesicle Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593Probable ubiquitin carboxyl-terminal hydrolase 4PRO_0000080605Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei338 – 3381Phosphoserine1 Publication
    Modified residuei343 – 3431Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO60139.

    Interactioni

    Subunit structurei

    Interacts with sfp47.1 Publication

    Protein-protein interaction databases

    BioGridi277355. 91 interactions.
    MINTiMINT-4677554.
    STRINGi4896.SPBC18H10.08c-1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini227 – 573347USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    KOiK11839.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60139-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDDYFDRLF ELAFVYINED ETIQSCSFRG QRWLEEAQTL EQKNSLLKAY    50
    YYYLKALKLA YEIPCRFEIS VKSTHYGEFK QFQKLAIQAV SKAFTIKSKL 100
    AVKHYLPVIQ ISDALSLSKK SSLKVLFLNF YSQESSKGYV FSKHTIAIPI 150
    SCLQSMDSSK IYDFLKSAPF HPSMVICYSL ERYFEDVSLA YKLYSMLRSL 200
    KLDPHFMELA NPKKVDSSLS YENYQPIGLT NLGNTCYMNC VLQCLFACKD 250
    LTIPMLQGRG LLQNINTKNP LGTGGKITSA FFSLLQSVLL NHGQRSISPR 300
    NFLEIVQSLN RDFSIDGQCD AQEFLNFFLD KLHEDLNSNA SRSPIAPLTE 350
    DQLSAREELP LSHFSHIEWN LHLRSNKSIV VNNFVGQLCS RTQCMTCGRT 400
    STTFAPFTSL AIPIDDVSHV VSLQECLLKF SAPELLQGHD GWHCPVCKVQ 450
    RSAKKVIMIS KLPEYLIIQI QRFKISVMGR KKIDTPLGLS LQIPSKMLVP 500
    PSFQSGIGYI PSNYNLFAFI CHYGQLENGH YISDVLFNNE WCHIDDSIVR 550
    TVGGITDLRE DFSSSYILFY KRSSLLEEFE DKCPKMTLKR NVK 593
    Length:593
    Mass (Da):67,747
    Last modified:April 18, 2012 - v2
    Checksum:i16801C84BB258B61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18405.2.
    PIRiT39772.
    RefSeqiNP_595732.2. NM_001021630.2.

    Genome annotation databases

    EnsemblFungiiSPBC18H10.08c.1; SPBC18H10.08c.1:pep; SPBC18H10.08c.
    GeneIDi2540837.
    KEGGispo:SPBC18H10.08c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18405.2 .
    PIRi T39772.
    RefSeqi NP_595732.2. NM_001021630.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277355. 91 interactions.
    MINTi MINT-4677554.
    STRINGi 4896.SPBC18H10.08c-1.

    Protein family/group databases

    MEROPSi C19.A58.

    Proteomic databases

    MaxQBi O60139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC18H10.08c.1 ; SPBC18H10.08c.1:pep ; SPBC18H10.08c .
    GeneIDi 2540837.
    KEGGi spo:SPBC18H10.08c.

    Organism-specific databases

    PomBasei SPBC18H10.08c.

    Phylogenomic databases

    eggNOGi COG5533.
    KOi K11839.
    OrthoDBi EOG7R2BSX.

    Miscellaneous databases

    NextBioi 20801954.
    PROi O60139.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND SER-343, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity."
      Kouranti I., McLean J.R., Feoktistova A., Liang P., Johnson A.E., Roberts-Galbraith R.H., Gould K.L.
      PLoS Biol. 8:708-716(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SFP47, SUBCELLULAR LOCATION.
    5. "Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability."
      Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L., Hagan I.M., Miller C.J.
      Genetics 187:1207-1217(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.

    Entry informationi

    Entry nameiUBP4_SCHPO
    AccessioniPrimary (citable) accession number: O60139
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: April 18, 2012
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3