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O60139 (UBP4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable ubiquitin carboxyl-terminal hydrolase 4

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene names
Name:ubp4
ORF Names:SPBC18H10.08c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Acts late in the proteolytic pathway in conjunction with the 26S proteasome. Plays a role in avoiding DNA overreplication By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with sfp47. Ref.4

Subcellular location

Cytoplasm. Endosome Ref.2 Ref.4.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 593593Probable ubiquitin carboxyl-terminal hydrolase 4
PRO_0000080605

Regions

Domain227 – 573347USP

Sites

Active site2361Nucleophile By similarity
Active site5301Proton acceptor By similarity

Amino acid modifications

Modified residue3381Phosphoserine Ref.3
Modified residue3431Phosphoserine Ref.3

Sequences

Sequence LengthMass (Da)Tools
O60139 [UniParc].

Last modified April 18, 2012. Version 2.
Checksum: 16801C84BB258B61

FASTA59367,747
        10         20         30         40         50         60 
MSDDYFDRLF ELAFVYINED ETIQSCSFRG QRWLEEAQTL EQKNSLLKAY YYYLKALKLA 

        70         80         90        100        110        120 
YEIPCRFEIS VKSTHYGEFK QFQKLAIQAV SKAFTIKSKL AVKHYLPVIQ ISDALSLSKK 

       130        140        150        160        170        180 
SSLKVLFLNF YSQESSKGYV FSKHTIAIPI SCLQSMDSSK IYDFLKSAPF HPSMVICYSL 

       190        200        210        220        230        240 
ERYFEDVSLA YKLYSMLRSL KLDPHFMELA NPKKVDSSLS YENYQPIGLT NLGNTCYMNC 

       250        260        270        280        290        300 
VLQCLFACKD LTIPMLQGRG LLQNINTKNP LGTGGKITSA FFSLLQSVLL NHGQRSISPR 

       310        320        330        340        350        360 
NFLEIVQSLN RDFSIDGQCD AQEFLNFFLD KLHEDLNSNA SRSPIAPLTE DQLSAREELP 

       370        380        390        400        410        420 
LSHFSHIEWN LHLRSNKSIV VNNFVGQLCS RTQCMTCGRT STTFAPFTSL AIPIDDVSHV 

       430        440        450        460        470        480 
VSLQECLLKF SAPELLQGHD GWHCPVCKVQ RSAKKVIMIS KLPEYLIIQI QRFKISVMGR 

       490        500        510        520        530        540 
KKIDTPLGLS LQIPSKMLVP PSFQSGIGYI PSNYNLFAFI CHYGQLENGH YISDVLFNNE 

       550        560        570        580        590 
WCHIDDSIVR TVGGITDLRE DFSSSYILFY KRSSLLEEFE DKCPKMTLKR NVK 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND SER-343, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity."
Kouranti I., McLean J.R., Feoktistova A., Liang P., Johnson A.E., Roberts-Galbraith R.H., Gould K.L.
PLoS Biol. 8:708-716(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SFP47, SUBCELLULAR LOCATION.
[5]"Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability."
Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L., Hagan I.M., Miller C.J.
Genetics 187:1207-1217(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA18405.2.
PIRT39772.
RefSeqNP_595732.2. NM_001021630.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277355. 91 interactions.
MINTMINT-4677554.
STRING4896.SPBC18H10.08c-1.

Protein family/group databases

MEROPSC19.A58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC18H10.08c.1; SPBC18H10.08c.1:pep; SPBC18H10.08c.
GeneID2540837.
KEGGspo:SPBC18H10.08c.

Organism-specific databases

PomBaseSPBC18H10.08c.

Phylogenomic databases

eggNOGCOG5533.
KOK11839.
OrthoDBEOG7R2BSX.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801954.
PROO60139.

Entry information

Entry nameUBP4_SCHPO
AccessionPrimary (citable) accession number: O60139
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: April 18, 2012
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Peptidase families

Classification of peptidase families and list of entries