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O60135

- LCF1_SCHPO

UniProt

O60135 - LCF1_SCHPO

Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

lcf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities By similarity. Appears to play a role in the maintenance of cell viability during stationary phase.By similarity1 Publication

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. myristoyl-CoA ligase activity Source: PomBase
    3. oleoyl-CoA ligase activity Source: PomBase
    4. palmitoyl-CoA ligase activity Source: PomBase

    GO - Biological processi

    1. long-chain fatty acid metabolic process Source: PomBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
    Alternative name(s):
    Fatty acid activator 1
    Long-chain acyl-CoA synthetase 1
    Gene namesi
    Name:lcf1
    ORF Names:SPBC18H10.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC18H10.02.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. endoplasmic reticulum Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 676676Long-chain-fatty-acid--CoA ligase 1PRO_0000193118Add
    BLAST

    Proteomic databases

    MaxQBiO60135.
    PaxDbiO60135.

    Interactioni

    Protein-protein interaction databases

    BioGridi277254. 4 interactions.
    MINTiMINT-4677511.
    STRINGi4896.SPBC18H10.02-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO60135.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    KOiK01897.
    OMAiGISVHNM.
    OrthoDBiEOG7CCC0K.
    PhylomeDBiO60135.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60135-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVQSKAISK PKEHESAIYR NANFPDHLVE TYSDDVHTLF DVFRHSVKQF    50
    GNKKAMGYRN LVKEHVETKM VTKVVDGEKK EVPKSWSYFE LSDYNYLSFN 100
    DIYDKALRYA GALRKLGLNK GDKFELYAPT SAFWLLTAEA CLSQSMTIVT 150
    AYDTLGEEGL LHSLRESGVR GMYTEGHLLK TLVNPLKEIE SLEVIIYRND 200
    AKEEDIKTIQ EIRPNLKLIK FADFEKMSPP VEPDPPSPEE ICCIMYTSGS 250
    TGLPKGVILS HKNMVAIVTA IVKHVPEVTS KDYLLAYLPL AHILEFAFEN 300
    ICLAWGGTIG YANVRTLVDT NCRNCKGDIN TFRPTIMVGV PAVWEMVRKG 350
    IMSKLNAASA VKRSVFWTAY YTKAKLMRHN LPGSCVLDTA VFNKIRSMGT 400
    GGRLRYTLSG GSALSPDTKR FLSIVLCPML IGYGLTEISA AAMVQNPACF 450
    NLDDSAGSLL PCTEMKLVDC EEGNYNSHGH PPRGEIWLRG PSLTRGYLNR 500
    DKENKESFTP DGWFRTGDVG ELTPEGLLRI IDRKKNLVKT QNGEYIALEK 550
    LESRYRTSSL VSNICVYADQ TKVKPLAIIV PNEPVVRKLA TEQAGLSPDA 600
    SWEEVCHNKK VRQLVYDDLI RIGRSHHFAN IELIQNVVLV PIEFTPENGL 650
    VTAAQKLQRR KILDRFKKEI DAAYAE 676
    Length:676
    Mass (Da):75,966
    Last modified:August 1, 1998 - v1
    Checksum:i98EF74D4A1C5AA41
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18399.1.
    PIRiT39766.
    RefSeqiNP_595726.1. NM_001021624.2.

    Genome annotation databases

    EnsemblFungiiSPBC18H10.02.1; SPBC18H10.02.1:pep; SPBC18H10.02.
    GeneIDi2540731.
    KEGGispo:SPBC18H10.02.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18399.1 .
    PIRi T39766.
    RefSeqi NP_595726.1. NM_001021624.2.

    3D structure databases

    ProteinModelPortali O60135.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277254. 4 interactions.
    MINTi MINT-4677511.
    STRINGi 4896.SPBC18H10.02-1.

    Proteomic databases

    MaxQBi O60135.
    PaxDbi O60135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC18H10.02.1 ; SPBC18H10.02.1:pep ; SPBC18H10.02 .
    GeneIDi 2540731.
    KEGGi spo:SPBC18H10.02.

    Organism-specific databases

    PomBasei SPBC18H10.02.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    KOi K01897.
    OMAi GISVHNM.
    OrthoDBi EOG7CCC0K.
    PhylomeDBi O60135.

    Miscellaneous databases

    NextBioi 20801853.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A defect in a fatty acyl-CoA synthetase gene, lcf1+, results in a decrease in viability after entry into the stationary phase in fission yeast."
      Oshiro T., Aiba H., Mizuno T.
      Mol. Genet. Genomics 269:437-442(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiLCF1_SCHPO
    AccessioniPrimary (citable) accession number: O60135
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3