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O60135

- LCF1_SCHPO

UniProt

O60135 - LCF1_SCHPO

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Protein
Long-chain-fatty-acid--CoA ligase 1
Gene
lcf1, SPBC18H10.02
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities By similarity. Appears to play a role in the maintenance of cell viability during stationary phase.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Magnesium By similarity.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. myristoyl-CoA ligase activity Source: PomBase
  3. oleoyl-CoA ligase activity Source: PomBase
  4. palmitoyl-CoA ligase activity Source: PomBase

GO - Biological processi

  1. long-chain fatty acid metabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 1
Long-chain acyl-CoA synthetase 1
Gene namesi
Name:lcf1
ORF Names:SPBC18H10.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC18H10.02.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. endoplasmic reticulum Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 676676Long-chain-fatty-acid--CoA ligase 1
PRO_0000193118Add
BLAST

Proteomic databases

MaxQBiO60135.
PaxDbiO60135.

Interactioni

Protein-protein interaction databases

BioGridi277254. 4 interactions.
MINTiMINT-4677511.
STRINGi4896.SPBC18H10.02-1.

Structurei

3D structure databases

ProteinModelPortaliO60135.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
KOiK01897.
OMAiGISVHNM.
OrthoDBiEOG7CCC0K.
PhylomeDBiO60135.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60135-1 [UniParc]FASTAAdd to Basket

« Hide

MKVQSKAISK PKEHESAIYR NANFPDHLVE TYSDDVHTLF DVFRHSVKQF    50
GNKKAMGYRN LVKEHVETKM VTKVVDGEKK EVPKSWSYFE LSDYNYLSFN 100
DIYDKALRYA GALRKLGLNK GDKFELYAPT SAFWLLTAEA CLSQSMTIVT 150
AYDTLGEEGL LHSLRESGVR GMYTEGHLLK TLVNPLKEIE SLEVIIYRND 200
AKEEDIKTIQ EIRPNLKLIK FADFEKMSPP VEPDPPSPEE ICCIMYTSGS 250
TGLPKGVILS HKNMVAIVTA IVKHVPEVTS KDYLLAYLPL AHILEFAFEN 300
ICLAWGGTIG YANVRTLVDT NCRNCKGDIN TFRPTIMVGV PAVWEMVRKG 350
IMSKLNAASA VKRSVFWTAY YTKAKLMRHN LPGSCVLDTA VFNKIRSMGT 400
GGRLRYTLSG GSALSPDTKR FLSIVLCPML IGYGLTEISA AAMVQNPACF 450
NLDDSAGSLL PCTEMKLVDC EEGNYNSHGH PPRGEIWLRG PSLTRGYLNR 500
DKENKESFTP DGWFRTGDVG ELTPEGLLRI IDRKKNLVKT QNGEYIALEK 550
LESRYRTSSL VSNICVYADQ TKVKPLAIIV PNEPVVRKLA TEQAGLSPDA 600
SWEEVCHNKK VRQLVYDDLI RIGRSHHFAN IELIQNVVLV PIEFTPENGL 650
VTAAQKLQRR KILDRFKKEI DAAYAE 676
Length:676
Mass (Da):75,966
Last modified:August 1, 1998 - v1
Checksum:i98EF74D4A1C5AA41
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA18399.1.
PIRiT39766.
RefSeqiNP_595726.1. NM_001021624.2.

Genome annotation databases

EnsemblFungiiSPBC18H10.02.1; SPBC18H10.02.1:pep; SPBC18H10.02.
GeneIDi2540731.
KEGGispo:SPBC18H10.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329671 Genomic DNA. Translation: CAA18399.1 .
PIRi T39766.
RefSeqi NP_595726.1. NM_001021624.2.

3D structure databases

ProteinModelPortali O60135.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277254. 4 interactions.
MINTi MINT-4677511.
STRINGi 4896.SPBC18H10.02-1.

Proteomic databases

MaxQBi O60135.
PaxDbi O60135.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC18H10.02.1 ; SPBC18H10.02.1:pep ; SPBC18H10.02 .
GeneIDi 2540731.
KEGGi spo:SPBC18H10.02.

Organism-specific databases

PomBasei SPBC18H10.02.

Phylogenomic databases

eggNOGi COG1022.
HOGENOMi HOG000159459.
KOi K01897.
OMAi GISVHNM.
OrthoDBi EOG7CCC0K.
PhylomeDBi O60135.

Miscellaneous databases

NextBioi 20801853.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A defect in a fatty acyl-CoA synthetase gene, lcf1+, results in a decrease in viability after entry into the stationary phase in fission yeast."
    Oshiro T., Aiba H., Mizuno T.
    Mol. Genet. Genomics 269:437-442(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiLCF1_SCHPO
AccessioniPrimary (citable) accession number: O60135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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