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Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

lcf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities (By similarity). Appears to play a role in the maintenance of cell viability during stationary phase.By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • myristoyl-CoA ligase activity Source: PomBase
  • oleoyl-CoA ligase activity Source: PomBase
  • palmitoyl-CoA ligase activity Source: PomBase

GO - Biological processi

  • long-chain fatty-acyl-CoA metabolic process Source: PomBase

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-75876 Synthesis of very long-chain fatty acyl-CoAs

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 1
Long-chain acyl-CoA synthetase 1
Gene namesi
Name:lcf1
ORF Names:SPBC18H10.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC18H10.02
PomBaseiSPBC18H10.02 lcf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931181 – 676Long-chain-fatty-acid--CoA ligase 1Add BLAST676

Proteomic databases

MaxQBiO60135
PaxDbiO60135
PRIDEiO60135

Interactioni

Protein-protein interaction databases

BioGridi277254, 3 interactors
STRINGi4896.SPBC18H10.02.1

Structurei

3D structure databases

ProteinModelPortaliO60135
SMRiO60135
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000159459
InParanoidiO60135
KOiK01897
OMAiSVLPIWH
OrthoDBiEOG092C0RYI
PhylomeDBiO60135

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

O60135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVQSKAISK PKEHESAIYR NANFPDHLVE TYSDDVHTLF DVFRHSVKQF
60 70 80 90 100
GNKKAMGYRN LVKEHVETKM VTKVVDGEKK EVPKSWSYFE LSDYNYLSFN
110 120 130 140 150
DIYDKALRYA GALRKLGLNK GDKFELYAPT SAFWLLTAEA CLSQSMTIVT
160 170 180 190 200
AYDTLGEEGL LHSLRESGVR GMYTEGHLLK TLVNPLKEIE SLEVIIYRND
210 220 230 240 250
AKEEDIKTIQ EIRPNLKLIK FADFEKMSPP VEPDPPSPEE ICCIMYTSGS
260 270 280 290 300
TGLPKGVILS HKNMVAIVTA IVKHVPEVTS KDYLLAYLPL AHILEFAFEN
310 320 330 340 350
ICLAWGGTIG YANVRTLVDT NCRNCKGDIN TFRPTIMVGV PAVWEMVRKG
360 370 380 390 400
IMSKLNAASA VKRSVFWTAY YTKAKLMRHN LPGSCVLDTA VFNKIRSMGT
410 420 430 440 450
GGRLRYTLSG GSALSPDTKR FLSIVLCPML IGYGLTEISA AAMVQNPACF
460 470 480 490 500
NLDDSAGSLL PCTEMKLVDC EEGNYNSHGH PPRGEIWLRG PSLTRGYLNR
510 520 530 540 550
DKENKESFTP DGWFRTGDVG ELTPEGLLRI IDRKKNLVKT QNGEYIALEK
560 570 580 590 600
LESRYRTSSL VSNICVYADQ TKVKPLAIIV PNEPVVRKLA TEQAGLSPDA
610 620 630 640 650
SWEEVCHNKK VRQLVYDDLI RIGRSHHFAN IELIQNVVLV PIEFTPENGL
660 670
VTAAQKLQRR KILDRFKKEI DAAYAE
Length:676
Mass (Da):75,966
Last modified:August 1, 1998 - v1
Checksum:i98EF74D4A1C5AA41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA18399.1
PIRiT39766
RefSeqiNP_595726.1, NM_001021624.2

Genome annotation databases

EnsemblFungiiSPBC18H10.02.1; SPBC18H10.02.1:pep; SPBC18H10.02
GeneIDi2540731
KEGGispo:SPBC18H10.02

Similar proteinsi

Entry informationi

Entry nameiLCF1_SCHPO
AccessioniPrimary (citable) accession number: O60135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health