Reviewed, UniProtKB/Swiss-Prot O60132 (TEA4_SCHPO)

Names and origin

Protein names

Recommended name:
    Tip elongation aberrant protein Tea4
Alternative name(s):
    Cell polarity protein tea4
    Win1-interacting SH3 domain protein

Gene names

Name:	tea4
Synonyms:	wsh3
ORF Names:	SPBC1706.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell polarity factor essential for the bipolar localization and function of structures containing the cell-end marker tea1 during the normal cell cycle. Regulates cell polarity in complex with tea1 and together with the stress signaling MAPK cascade, contributes to cell polarity maintenance under stress conditions. Required for the localization of for3 at the cell tip specifically during initiation of bipolar growth. During the new end take off (NETO), formation of a protein complex that includes tea1, tea4 and for3 is necessary and sufficient for the establishment of cell polarity and localized actin assembly at new cell ends. Ref.2 Ref.3
Subunit structure	An essential component of the tea1 cell-end complex. Interacts with win1, tea1 and for3. Interacts with tip1 in the presence of tea1. Ref.2 Ref.3
Subcellular location	Note: Through it's binding with tea1, is transported by the cytoplasmic microtubule system and is localized at cell tips, microtubule plus ends and cytoplasmic dots. Ref.2 Ref.3
Sequence similarities	Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of bipolar cell growth Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe cellular protein localization Ref.2 Ref.3 Inferred from genetic interaction. Source: GeneDB_SPombe establishment or maintenance of actin cytoskeleton polarity Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Ref.2 Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of filamentous growth Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	Tea1 cell-end complex Ref.2 Inferred from direct assay. Source: GeneDB_SPombe cell cortex of cell tip Ref.3 Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding, bridging Ref.3 Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
for3	O94532	2	EBI-1099982,EBI-1102572
tea1	P87061	5	EBI-1099982,EBI-875376
tip1	P79065	1	EBI-1099982,EBI-1102463
win1	O74304	1	EBI-1099982,EBI-1099995

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Tip elongation aberrant protein Tea4		PRO_0000259412
Regions
	Domain	118 – 179	62	SH3
	Region	515 – 809	295	Interaction with tea1 Ref.3
	Region	587 – 809	223	Interaction with win1 Ref.2
	Compositional bias	96 – 99	4	Poly-Glu
	Compositional bias	260 – 692	433	Ser-rich
Amino acid modifications
	Modified residue	23	1	Phosphotyrosine Ref.4
	Modified residue	24	1	Phosphoserine Ref.4
	Modified residue	28	1	Phosphotyrosine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O60132-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2D1386C6BE0A046B	FASTA	809	89,562
	10 20 30 40 50 60 MEIMESHFDP TQQNDSTIIE SRYSPEEYLE QSFEIQRIIS GENSEPQTVA SQEISDSQEE 70 80 90 100 110 120 DTTLTSSQFE DCGTEYNEVV EDDEFRSEDE DDFMDEEEEY ALYEAELSSS PSIHEEVIDC 130 140 150 160 170 180 NFVHAIRGFE ATVEGQVDAT KGDMMILLDD SNSYWWLVKM CKNLAIGYLP AEYIETPSER 190 200 210 220 230 240 LARLNKYKNS ETSNSQQSVT LPPLDIVEKT LEAPSPNFRI KRVTFTCSSN SSDDEMDSEN 250 260 270 280 290 300 DYEAMVNRTV AENGLEIEFS DSSDSSLSAE YRSESEDHVT DSPAYVDLTE LEGGFNQFNS 310 320 330 340 350 360 TSFQSTSPLG LEIVETEING SSTTADSKNS HSPYSKFSSA YPDAENSNIS KINISIAGNK 370 380 390 400 410 420 ELYGNATQSD PSLYSTWIAN KHKTASSATV DSPLRRSLSV DAMQSNASFS SYSSTSNTDK 430 440 450 460 470 480 SLRPSSYSAV SESSNFTHDV SRDNKEISLN APKSIIVSQS DSFDTSNVTQ DAPNDVEKEP 490 500 510 520 530 540 ISGQMPNNLS VQSLKQLEVY PIRHSVSIEM PSEKLLSPRL YSSSTPSSPT KGFQKDDEED 550 560 570 580 590 600 SENRKQADKV ELSPSSLLRQ MSLPVDSSSQ SDAQCTTSSV YITAERKAFS QSSIDLSTLS 610 620 630 640 650 660 NHHVNNEINR RSFAGGFTSL ADELSEMREL LHESPAPLEC NEEMVIPTPE LDASSAIPSS 670 680 690 700 710 720 SISHDEDLLP RKNTEESTSS SSFSSLITSP ASLQYDENPF KQSVVAELNN NSSSVPFVDS 730 740 750 760 770 780 AHASDIHAYD NDHVSTKNKE FNRRLREFIL DPDSLSGLYW SVKSAGVRAS RRVSRNIEGE 790 800 SVSSDLDDIF ANVLKGLSDE MASLLNTNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Wsh3/Tea4 is a novel cell-end factor essential for bipolar distribution of Tea1 and protects cell polarity under environmental stress in S. pombe." Tatebe H., Shimada K., Uzawa S., Morigasaki S., Shiozaki K. Curr. Biol. 15:1006-1015(2005) [PubMed: 15936270] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TEA1 AND WIN1.
[3]	"Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity." Martin S.G., McDonald W.H., Yates J.R. III, Chang F. Dev. Cell 8:479-491(2005) [PubMed: 15809031] [Abstract] Cited for: FUNCTION, INTERACTION WITH FOR3; TEA1 AND TIP1, SUBCELLULAR LOCATION.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-23; SER-24 AND TYR-28, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA19002.1.
PIR	T39626.
RefSeq	NP_595240.1.
3D structure databases
HSSP	HSSP built from PDB template 1N5Z based on UniProtKB P80667.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60132. 4 interactions.
STRING	O60132.
Genome annotation databases
GeneID	2539642.
GenomeReviews	Gene locus tea4 in contig CU329671_GR.
KEGG	spo:SPBC1706.01.
NMPDR	fig|4896.1.peg.1106.
Organism-specific databases
GeneDB_Spombe	SPBC1706.01.
Phylogenomic databases
OrthoDB	EOG93V1C5.
Gene expression databases
ArrayExpress	O60132.
Family and domain databases
InterPro	IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TEA4_SCHPO

Accession

Primary (citable) accession number: O60132

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 1, 1998
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)