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O60126

- TOP3_SCHPO

UniProt

O60126 - TOP3_SCHPO

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Protein

DNA topoisomerase 3

Gene
top3, SPBC16G5.12c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei330 – 3301O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
  4. protein binding Source: PomBase

GO - Biological processi

  1. DNA metabolic process Source: PomBase
  2. DNA topological change Source: InterPro
  3. maintenance of rDNA Source: PomBase
  4. mitotic sister chromatid segregation Source: PomBase
  5. nuclear cell cycle DNA replication Source: PomBase
  6. nucleolus organization Source: PomBase
  7. postreplication repair Source: PomBase
  8. resolution of meiotic recombination intermediates Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:top3
ORF Names:SPBC16G5.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC16G5.12c.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: PomBase
  2. nucleus Source: PomBase
  3. RecQ helicase-Topo III complex Source: PomBase
  4. site of double-strand break Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622DNA topoisomerase 3PRO_0000145197Add
BLAST

Proteomic databases

MaxQBiO60126.

Interactioni

Subunit structurei

Interacts with hus2.1 Publication

Protein-protein interaction databases

BioGridi276427. 12 interactions.
MINTiMINT-4677412.
STRINGi4896.SPBC16G5.12c-1.

Structurei

3D structure databases

ProteinModelPortaliO60126.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 148147ToprimAdd
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000184377.
KOiK03165.
OMAiRTECINE.
OrthoDBiEOG7Q8CWW.
PhylomeDBiO60126.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60126-1 [UniParc]FASTAAdd to Basket

« Hide

MRVLCVAEKN SIAKSVASIL GGGHVRRRDT RSKYVKNYDF SFNFGGNVGS    50
SDVTMTSVSG HLTEASFPSE YSSWSSVPQD VLFDAQIITS VSKNAEVLAD 100
NIKKEARNAQ YLYIWTDCDR EGEHIGVEIS NVARASNPSI QVIRADFNNL 150
ERSHIISAAK RPRDVSKNAA DAVDARIELD FRLGAIFTRL QTIQLQKSFD 200
ILQNKIISYG PCQFPTLGFV VDRWQRVEDF VPETYWHLRF VDKRQGKTIQ 250
FNWERAKVFD RLTTMIILEN CLECKTAKVV NITQKPKTKY KPLPLSTVEL 300
TKLGPKHLRI SAKKTLELAE NLYTNGFVSY PRTETDQFDS SMNLHAIIQK 350
LTGAQEWDSY AEGLLAGDYR PPRKGKHNDR AHPPIHPVQM VHRSALPSQD 400
HWKVYELITR RFLACCSDNA KGAETLVQVK MEEELFSKKG LLVTEKNYLE 450
VYPYEKWESS DQLPEYRLHE EFQPHILDMM DSSTSSPSYI TEPELIALMD 500
ANGIGTDATM AEHIEKVQER EYVIKRKKRG QGVTEFVPSS LGVALAKGYD 550
EIGLEWSLTK PFLRKEMEVQ LKNIENGQLN RNVLVHMILT QFRDVFHLTK 600
QRFDCLKNSC RVYLMSHNEP QT 622
Length:622
Mass (Da):71,169
Last modified:May 1, 1999 - v2
Checksum:iEF291B1717EB58C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF126287 mRNA. Translation: AAD22485.2.
CU329671 Genomic DNA. Translation: CAA19038.1.
PIRiT39604.
RefSeqiNP_596761.1. NM_001023781.2.

Genome annotation databases

EnsemblFungiiSPBC16G5.12c.1; SPBC16G5.12c.1:pep; SPBC16G5.12c.
GeneIDi2539881.
KEGGispo:SPBC16G5.12c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF126287 mRNA. Translation: AAD22485.2 .
CU329671 Genomic DNA. Translation: CAA19038.1 .
PIRi T39604.
RefSeqi NP_596761.1. NM_001023781.2.

3D structure databases

ProteinModelPortali O60126.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276427. 12 interactions.
MINTi MINT-4677412.
STRINGi 4896.SPBC16G5.12c-1.

Proteomic databases

MaxQBi O60126.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC16G5.12c.1 ; SPBC16G5.12c.1:pep ; SPBC16G5.12c .
GeneIDi 2539881.
KEGGi spo:SPBC16G5.12c.

Organism-specific databases

PomBasei SPBC16G5.12c.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000184377.
KOi K03165.
OMAi RTECINE.
OrthoDBi EOG7Q8CWW.
PhylomeDBi O60126.

Miscellaneous databases

NextBioi 20801026.
PROi O60126.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The top3+ gene is essential in Schizosaccharomyces pombe and the lethality associated with its loss is caused by Rad12 helicase activity."
    Maftahi M., Han C.S., Langston L.D., Hope J.C., Zigouras N., Freyer G.A.
    Nucleic Acids Res. 27:4715-4724(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Role for the fission yeast RecQ helicase in DNA repair in G2."
    Laursen L.V., Ampatzidou E., Andersen A.H., Murray J.M.
    Mol. Cell. Biol. 23:3692-3705(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUS2.

Entry informationi

Entry nameiTOP3_SCHPO
AccessioniPrimary (citable) accession number: O60126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi