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O60126

- TOP3_SCHPO

UniProt

O60126 - TOP3_SCHPO

Protein

DNA topoisomerase 3

Gene

top3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (01 May 1999)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei330 – 3301O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. protein binding Source: PomBase

    GO - Biological processi

    1. DNA metabolic process Source: PomBase
    2. DNA topological change Source: InterPro
    3. maintenance of rDNA Source: PomBase
    4. mitotic sister chromatid segregation Source: PomBase
    5. nuclear cell cycle DNA replication Source: PomBase
    6. nucleolus organization Source: PomBase
    7. postreplication repair Source: PomBase
    8. resolution of meiotic recombination intermediates Source: PomBase

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III
    Gene namesi
    Name:top3
    ORF Names:SPBC16G5.12c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC16G5.12c.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: PomBase
    2. nucleus Source: PomBase
    3. RecQ helicase-Topo III complex Source: PomBase
    4. site of double-strand break Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622DNA topoisomerase 3PRO_0000145197Add
    BLAST

    Proteomic databases

    MaxQBiO60126.

    Interactioni

    Subunit structurei

    Interacts with hus2.1 Publication

    Protein-protein interaction databases

    BioGridi276427. 12 interactions.
    MINTiMINT-4677412.
    STRINGi4896.SPBC16G5.12c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO60126.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 148147ToprimPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000184377.
    KOiK03165.
    OMAiRTECINE.
    OrthoDBiEOG7Q8CWW.
    PhylomeDBiO60126.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60126-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLCVAEKN SIAKSVASIL GGGHVRRRDT RSKYVKNYDF SFNFGGNVGS    50
    SDVTMTSVSG HLTEASFPSE YSSWSSVPQD VLFDAQIITS VSKNAEVLAD 100
    NIKKEARNAQ YLYIWTDCDR EGEHIGVEIS NVARASNPSI QVIRADFNNL 150
    ERSHIISAAK RPRDVSKNAA DAVDARIELD FRLGAIFTRL QTIQLQKSFD 200
    ILQNKIISYG PCQFPTLGFV VDRWQRVEDF VPETYWHLRF VDKRQGKTIQ 250
    FNWERAKVFD RLTTMIILEN CLECKTAKVV NITQKPKTKY KPLPLSTVEL 300
    TKLGPKHLRI SAKKTLELAE NLYTNGFVSY PRTETDQFDS SMNLHAIIQK 350
    LTGAQEWDSY AEGLLAGDYR PPRKGKHNDR AHPPIHPVQM VHRSALPSQD 400
    HWKVYELITR RFLACCSDNA KGAETLVQVK MEEELFSKKG LLVTEKNYLE 450
    VYPYEKWESS DQLPEYRLHE EFQPHILDMM DSSTSSPSYI TEPELIALMD 500
    ANGIGTDATM AEHIEKVQER EYVIKRKKRG QGVTEFVPSS LGVALAKGYD 550
    EIGLEWSLTK PFLRKEMEVQ LKNIENGQLN RNVLVHMILT QFRDVFHLTK 600
    QRFDCLKNSC RVYLMSHNEP QT 622
    Length:622
    Mass (Da):71,169
    Last modified:May 1, 1999 - v2
    Checksum:iEF291B1717EB58C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126287 mRNA. Translation: AAD22485.2.
    CU329671 Genomic DNA. Translation: CAA19038.1.
    PIRiT39604.
    RefSeqiNP_596761.1. NM_001023781.2.

    Genome annotation databases

    EnsemblFungiiSPBC16G5.12c.1; SPBC16G5.12c.1:pep; SPBC16G5.12c.
    GeneIDi2539881.
    KEGGispo:SPBC16G5.12c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126287 mRNA. Translation: AAD22485.2 .
    CU329671 Genomic DNA. Translation: CAA19038.1 .
    PIRi T39604.
    RefSeqi NP_596761.1. NM_001023781.2.

    3D structure databases

    ProteinModelPortali O60126.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276427. 12 interactions.
    MINTi MINT-4677412.
    STRINGi 4896.SPBC16G5.12c-1.

    Proteomic databases

    MaxQBi O60126.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC16G5.12c.1 ; SPBC16G5.12c.1:pep ; SPBC16G5.12c .
    GeneIDi 2539881.
    KEGGi spo:SPBC16G5.12c.

    Organism-specific databases

    PomBasei SPBC16G5.12c.

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000184377.
    KOi K03165.
    OMAi RTECINE.
    OrthoDBi EOG7Q8CWW.
    PhylomeDBi O60126.

    Miscellaneous databases

    NextBioi 20801026.
    PROi O60126.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The top3+ gene is essential in Schizosaccharomyces pombe and the lethality associated with its loss is caused by Rad12 helicase activity."
      Maftahi M., Han C.S., Langston L.D., Hope J.C., Zigouras N., Freyer G.A.
      Nucleic Acids Res. 27:4715-4724(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Role for the fission yeast RecQ helicase in DNA repair in G2."
      Laursen L.V., Ampatzidou E., Andersen A.H., Murray J.M.
      Mol. Cell. Biol. 23:3692-3705(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUS2.

    Entry informationi

    Entry nameiTOP3_SCHPO
    AccessioniPrimary (citable) accession number: O60126
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3