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Protein

Alkaline phosphatase

Gene

SPBC14F5.13c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68MagnesiumBy similarity1
Metal bindingi68Zinc 2By similarity1
Active sitei115Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi166MagnesiumBy similarity1
Metal bindingi168MagnesiumBy similarity1
Metal bindingi306MagnesiumBy similarity1
Metal bindingi311Zinc 1By similarity1
Metal bindingi315Zinc 1By similarity1
Metal bindingi352Zinc 2By similarity1
Metal bindingi353Zinc 2By similarity1
Metal bindingi456Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-1483166. Synthesis of PA.
R-SPO-8935690. Miscellaneous digestion events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Gene namesi
ORF Names:SPBC14F5.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.13c.
PomBaseiSPBC14F5.13c.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei27 – 47Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

  • fungal-type vacuole Source: PomBase
  • fungal-type vacuole membrane Source: PomBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861651 – 532Alkaline phosphataseAdd BLAST532

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60109.
PRIDEiO60109.

Interactioni

Protein-protein interaction databases

BioGridi276470. 13 interactors.
MINTiMINT-4677261.

Structurei

3D structure databases

ProteinModelPortaliO60109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000099116.
InParanoidiO60109.
KOiK01077.
OMAiLKSYNGA.
OrthoDBiEOG092C1UXR.
PhylomeDBiO60109.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASERDPLLP VHGEGPESPS RRNWKTWIKH GILLILVLST VIFFYFFSSH
60 70 80 90 100
KSKGTNEKPK FVIMMVSDGM GPGSLSMTRS FVETLNDKEG YRLPLDEHLI
110 120 130 140 150
GSSRTRSSSS LITDSAAGAT AFSCANKTYN GAVGVLDNEK PCGTILEAAK
160 170 180 190 200
EAGYLTGIVV TSRVTDATPA SFSAHAANRF MQDLIAEYQV GMGPLGRSVD
210 220 230 240 250
LLFGGGLCSF LPKSTYRSCR SDNLDLLKYA RKKEGFQILL NRTDFDELSN
260 270 280 290 300
AQLPLLGLFS DYHLSYDIDY QPEVQPKLSE MVETALDVLL NATNEDTSKG
310 320 330 340 350
FFLLIEGSRI DMASHNNDPI AHVYEVMEYN RAFEIASAFV EKNGGSLIST
360 370 380 390 400
SDHETGGLTV GRQVSKKYPE YLWKPQVLSL ALHSIEYLAS AIVNHNQNTL
410 420 430 440 450
LPYIEQFVLP AIGIPDPNPK QIHDIYVARH NIFNLINVLS DIVSVEAQIG
460 470 480 490 500
WTTHGHTAVD VNVYGVGEVT EHLRGNMENI EIGQFMEIYL NVSLSDVTEK
510 520 530
LKDAPIHGAP DRPSLVETSF SDRLVGFGAD LF
Length:532
Mass (Da):58,666
Last modified:August 1, 1998 - v1
Checksum:i57A84A66926D545C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti513 – 514PS → HC in AAK07179 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316541 Genomic DNA. Translation: AAK07179.1.
CU329671 Genomic DNA. Translation: CAA19331.1.
PIRiT39459.
RefSeqiNP_596739.1. NM_001022665.2.

Genome annotation databases

EnsemblFungiiSPBC14F5.13c.1; SPBC14F5.13c.1:pep; SPBC14F5.13c.
GeneIDi2539926.
KEGGispo:SPBC14F5.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316541 Genomic DNA. Translation: AAK07179.1.
CU329671 Genomic DNA. Translation: CAA19331.1.
PIRiT39459.
RefSeqiNP_596739.1. NM_001022665.2.

3D structure databases

ProteinModelPortaliO60109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276470. 13 interactors.
MINTiMINT-4677261.

Proteomic databases

MaxQBiO60109.
PRIDEiO60109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14F5.13c.1; SPBC14F5.13c.1:pep; SPBC14F5.13c.
GeneIDi2539926.
KEGGispo:SPBC14F5.13c.

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.13c.
PomBaseiSPBC14F5.13c.

Phylogenomic databases

HOGENOMiHOG000099116.
InParanoidiO60109.
KOiK01077.
OMAiLKSYNGA.
OrthoDBiEOG092C1UXR.
PhylomeDBiO60109.

Enzyme and pathway databases

ReactomeiR-SPO-1483166. Synthesis of PA.
R-SPO-8935690. Miscellaneous digestion events.

Miscellaneous databases

PROiO60109.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_SCHPO
AccessioniPrimary (citable) accession number: O60109
Secondary accession number(s): Q9C427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.