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O60109

- PPB_SCHPO

UniProt

O60109 - PPB_SCHPO

Protein

Alkaline phosphatase

Gene

SPBC14F5.13c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681MagnesiumBy similarity
    Metal bindingi68 – 681Zinc 2By similarity
    Active sitei115 – 1151Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi166 – 1661MagnesiumBy similarity
    Metal bindingi168 – 1681MagnesiumBy similarity
    Metal bindingi306 – 3061MagnesiumBy similarity
    Metal bindingi311 – 3111Zinc 1By similarity
    Metal bindingi315 – 3151Zinc 1By similarity
    Metal bindingi352 – 3521Zinc 2By similarity
    Metal bindingi353 – 3531Zinc 2By similarity
    Metal bindingi456 – 4561Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: PomBase
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. biological regulation Source: PomBase
    2. protein dephosphorylation Source: PomBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase (EC:3.1.3.1)
    Gene namesi
    ORF Names:SPBC14F5.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC14F5.13c.

    Subcellular locationi

    GO - Cellular componenti

    1. fungal-type vacuole Source: PomBase
    2. fungal-type vacuole membrane Source: PomBase
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Alkaline phosphatasePRO_0000186165Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO60109.

    Interactioni

    Protein-protein interaction databases

    BioGridi276470. 11 interactions.
    MINTiMINT-4677261.
    STRINGi4896.SPBC14F5.13c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO60109.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei27 – 4721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099116.
    KOiK01077.
    OMAiLKSYNGA.
    OrthoDBiEOG71K6C1.
    PhylomeDBiO60109.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 2 hits.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60109-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASERDPLLP VHGEGPESPS RRNWKTWIKH GILLILVLST VIFFYFFSSH    50
    KSKGTNEKPK FVIMMVSDGM GPGSLSMTRS FVETLNDKEG YRLPLDEHLI 100
    GSSRTRSSSS LITDSAAGAT AFSCANKTYN GAVGVLDNEK PCGTILEAAK 150
    EAGYLTGIVV TSRVTDATPA SFSAHAANRF MQDLIAEYQV GMGPLGRSVD 200
    LLFGGGLCSF LPKSTYRSCR SDNLDLLKYA RKKEGFQILL NRTDFDELSN 250
    AQLPLLGLFS DYHLSYDIDY QPEVQPKLSE MVETALDVLL NATNEDTSKG 300
    FFLLIEGSRI DMASHNNDPI AHVYEVMEYN RAFEIASAFV EKNGGSLIST 350
    SDHETGGLTV GRQVSKKYPE YLWKPQVLSL ALHSIEYLAS AIVNHNQNTL 400
    LPYIEQFVLP AIGIPDPNPK QIHDIYVARH NIFNLINVLS DIVSVEAQIG 450
    WTTHGHTAVD VNVYGVGEVT EHLRGNMENI EIGQFMEIYL NVSLSDVTEK 500
    LKDAPIHGAP DRPSLVETSF SDRLVGFGAD LF 532
    Length:532
    Mass (Da):58,666
    Last modified:August 1, 1998 - v1
    Checksum:i57A84A66926D545C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti513 – 5142PS → HC in AAK07179. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF316541 Genomic DNA. Translation: AAK07179.1.
    CU329671 Genomic DNA. Translation: CAA19331.1.
    PIRiT39459.
    RefSeqiNP_596739.1. NM_001022665.2.

    Genome annotation databases

    EnsemblFungiiSPBC14F5.13c.1; SPBC14F5.13c.1:pep; SPBC14F5.13c.
    GeneIDi2539926.
    KEGGispo:SPBC14F5.13c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF316541 Genomic DNA. Translation: AAK07179.1 .
    CU329671 Genomic DNA. Translation: CAA19331.1 .
    PIRi T39459.
    RefSeqi NP_596739.1. NM_001022665.2.

    3D structure databases

    ProteinModelPortali O60109.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276470. 11 interactions.
    MINTi MINT-4677261.
    STRINGi 4896.SPBC14F5.13c-1.

    Proteomic databases

    MaxQBi O60109.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC14F5.13c.1 ; SPBC14F5.13c.1:pep ; SPBC14F5.13c .
    GeneIDi 2539926.
    KEGGi spo:SPBC14F5.13c.

    Organism-specific databases

    PomBasei SPBC14F5.13c.

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099116.
    KOi K01077.
    OMAi LKSYNGA.
    OrthoDBi EOG71K6C1.
    PhylomeDBi O60109.

    Miscellaneous databases

    NextBioi 20801069.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 2 hits.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of alkaline phosphatase gene from Schizosaccharomyces pombe."
      Kang S.-W., Lim C.-J.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiPPB_SCHPO
    AccessioniPrimary (citable) accession number: O60109
    Secondary accession number(s): Q9C427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3