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Protein

ERAD-associated E3 ubiquitin-protein ligase doa10

Gene

doa10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated E2 ligases, and transfers it to substrates promoting their degradation. Mediates the degradation of a broad range of substrates, including endoplasmic reticulum membrane proteins (ERQC), soluble nuclear proteins and soluble cytoplasmic proteins (CytoQC). Component of the doa10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. Also recognizes the N-terminally acetylated residue of proteins as degradation signal (degron) (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 61RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
ERAD-associated E3 ubiquitin-protein ligase doa10 (EC:6.3.2.-)
Gene namesi
Name:doa10
Synonyms:ssm4
ORF Names:SPBC14F5.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.07.
PomBaseiSPBC14F5.07. doa10.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 89CytoplasmicBy similarityAdd BLAST89
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 165LumenalBy similarityAdd BLAST55
Transmembranei166 – 186HelicalSequence analysisAdd BLAST21
Topological domaini187 – 453CytoplasmicBy similarityAdd BLAST267
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 495LumenalBy similarityAdd BLAST21
Transmembranei496 – 516HelicalSequence analysisAdd BLAST21
Topological domaini517 – 578CytoplasmicBy similarityAdd BLAST62
Transmembranei579 – 599HelicalSequence analysisAdd BLAST21
Topological domaini600 – 612LumenalBy similarityAdd BLAST13
Transmembranei613 – 633HelicalSequence analysisAdd BLAST21
Topological domaini634 – 663CytoplasmicBy similarityAdd BLAST30
Transmembranei664 – 684HelicalSequence analysisAdd BLAST21
Topological domaini685 – 710LumenalBy similarityAdd BLAST26
Transmembranei711 – 731HelicalSequence analysisAdd BLAST21
Topological domaini732 – 771CytoplasmicBy similarityAdd BLAST40
Transmembranei772 – 792HelicalSequence analysisAdd BLAST21
Topological domaini793 – 806LumenalBy similarityAdd BLAST14
Transmembranei807 – 827HelicalSequence analysisAdd BLAST21
Topological domaini828 – 961CytoplasmicBy similarityAdd BLAST134
Transmembranei962 – 982HelicalSequence analysisAdd BLAST21
Topological domaini983 – 1002LumenalBy similarityAdd BLAST20
Transmembranei1003 – 1023HelicalSequence analysisAdd BLAST21
Topological domaini1024 – 1054CytoplasmicBy similarityAdd BLAST31
Transmembranei1055 – 1075HelicalSequence analysisAdd BLAST21
Topological domaini1076 – 1089LumenalBy similarityAdd BLAST14
Transmembranei1090 – 1110HelicalSequence analysisAdd BLAST21
Topological domaini1111 – 1143CytoplasmicBy similarityAdd BLAST33
Transmembranei1144 – 1164HelicalSequence analysisAdd BLAST21
Topological domaini1165 – 1179LumenalBy similarityAdd BLAST15
Transmembranei1180 – 1200HelicalSequence analysisAdd BLAST21
Topological domaini1201 – 1242CytoplasmicBy similarityAdd BLAST42

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003106921 – 1242ERAD-associated E3 ubiquitin-protein ligase doa10Add BLAST1242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60103.
PRIDEiO60103.

PTM databases

iPTMnetiO60103.

Interactioni

Protein-protein interaction databases

BioGridi276403. 8 interactors.
MINTiMINT-4677203.

Structurei

3D structure databases

ProteinModelPortaliO60103.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the DOA10/MARCH6 family.Curated
Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 61RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

InParanoidiO60103.
KOiK10661.
OMAiWIPFTIG.
OrthoDBiEOG092C0DLV.
PhylomeDBiO60103.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNADDEICRV CRCEGAPDSP LFHPCKCTGS IRYVHQECLV EWLGHSKKTH
60 70 80 90 100
CELCKAKFEF TKVYSESMPR TIPFTILCRK LASTLKQRVI FFTRVLLTFF
110 120 130 140 150
CWTVLLPLIF KHVWNLNFKI GDTYTIHARN KTFTAPQKPG YFESISQITS
160 170 180 190 200
SPRLNTLIAN TAEGQVLTFV VTFILITAFL VREWVLQNAV QVADELQGQQ
210 220 230 240 250
FENVNQNNQA QAAAAAAQNL REVREARQRL AMVMEHLRER QEQRNLELQR
260 270 280 290 300
NGSFEEIERA RQRFALLGDN IREPQEEEND VDVDEIFNRQ QLNQPALDLN
310 320 330 340 350
DANSSNSVPV EFNSLHSQNV DYRDEVDSLR PQFNVDEQSS ISHSSNASEN
360 370 380 390 400
IVDGAVTQAN GIESDFTRVD HEPIIVNNDD ENGNNESENE EVIEEDNLNR
410 420 430 440 450
NVIAEAQNQV VADEERNAVA RAAQIAEADD ADDFDGILEF LGLRGPITGF
460 470 480 490 500
LQNCLVIAFV VSVFLTTAVG IPYMSGRLMV EWILFIIHRP TFILRFILSF
510 520 530 540 550
VNILFDWTVG GAFNIVKILT KLPLLSTVFV KLKLQGIFSS SFQQVSNNMY
560 570 580 590 600
SWIYDHVFSS SDHAYESLIY YMKTGHKQVV QSFSIFPVFR VCQMFAVILK
610 620 630 640 650
DFVENYSNRP VDRVFTTLIG YCMFTFLGIS YLNRKQFLFN DPQIRNVELA
660 670 680 690 700
FREVLRQCGS IAKFGIIFSI ELVVFPIFCG ILLSMCLIGT FKKLAAENLL
710 720 730 740 750
NVMTVYPAQS IFLAWFIGIT FMFEFAVFIS MVRKIVRPGV LYFLRDPNDP
760 770 780 790 800
QFHPIREILE KPMLFQLKKI GFSAILYFAF IIGCVGSVIH LLKSTGIIFP
810 820 830 840 850
IEFTTKPAVF EAPIDLLALE ILIFLSIKLF KPLELTRSFW RTLVSTFCRC
860 870 880 890 900
LRLSSYVMGQ RYSDEEGYYP KQYFSFLRRI ISKPSDTENQ DDGDKQKAKK
910 920 930 940 950
DFVQDGFFLW CPSKDVVPVR QGAMLIPVTE NGYEIFGEKK KVEENADYTI
960 970 980 990 1000
TYAPSNFYKR LIALLLFCWI CSTLVTVLLV FVPLSLGRAI YAWCFPNVVK
1010 1020 1030 1040 1050
HDFYAYAIGF YSISFPMYAI HASVKFLKLD YLRSLMNKLN LKIVMRSLVM
1060 1070 1080 1090 1100
ALKYLLLAFL GIFILPLLLG AIWELYVAIP FRTIFNRGTL ALDAFQNWVI
1110 1120 1130 1140 1150
GLFMLRMIYF TVTSNEERFV SRLFQDAFRD RWTNPQILPL LKNVLIPFTS
1160 1170 1180 1190 1200
ALIAAVVLPS VFTYVTYPFL SSIFPSASKT LMYRLMHPIF LALLGLALLG
1210 1220 1230 1240
RRFVETSSKW SQGIRDDLYL VGTRLHNFGE SAPPAISESA EK
Length:1,242
Mass (Da):142,366
Last modified:August 1, 1998 - v1
Checksum:iB9BD14AD2D476008
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19325.1.
PIRiT39453.
RefSeqiNP_596733.1. NM_001022659.2.

Genome annotation databases

EnsemblFungiiSPBC14F5.07.1; SPBC14F5.07.1:pep; SPBC14F5.07.
GeneIDi2539855.
KEGGispo:SPBC14F5.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19325.1.
PIRiT39453.
RefSeqiNP_596733.1. NM_001022659.2.

3D structure databases

ProteinModelPortaliO60103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276403. 8 interactors.
MINTiMINT-4677203.

PTM databases

iPTMnetiO60103.

Proteomic databases

MaxQBiO60103.
PRIDEiO60103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14F5.07.1; SPBC14F5.07.1:pep; SPBC14F5.07.
GeneIDi2539855.
KEGGispo:SPBC14F5.07.

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.07.
PomBaseiSPBC14F5.07. doa10.

Phylogenomic databases

InParanoidiO60103.
KOiK10661.
OMAiWIPFTIG.
OrthoDBiEOG092C0DLV.
PhylomeDBiO60103.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiO60103.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOA10_SCHPO
AccessioniPrimary (citable) accession number: O60103
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.