Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O60101 (PGK_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk1
ORF Names:SPBC14F5.04c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from sequence orthology. Source: PomBase

glycolytic process

Inferred from sequence orthology. Source: PomBase

   Cellular_componentcytosol

Inferred from direct assay PubMed 16823372. Source: PomBase

nucleus

Inferred from direct assay PubMed 16823372. Source: PomBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from sequence orthology. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145889

Regions

Nucleotide binding370 – 3734ATP By similarity
Region24 – 263Substrate binding By similarity
Region63 – 664Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1221Substrate By similarity
Binding site1691Substrate By similarity
Binding site2171ATP By similarity
Binding site3101ATP; via carbonyl oxygen By similarity
Binding site3411ATP By similarity

Amino acid modifications

Modified residue751Phosphotyrosine Ref.2
Modified residue761Phosphoserine Ref.2
Modified residue1431Phosphoserine Ref.2
Modified residue1721Phosphoserine Ref.2
Modified residue1731Phosphoserine Ref.2
Modified residue1831Phosphoserine Ref.2
Modified residue2531Phosphoserine Ref.2
Modified residue2601Phosphoserine Ref.2
Modified residue2991Phosphothreonine Ref.2
Modified residue3281Phosphoserine Ref.2
Modified residue3511Phosphoserine Ref.2
Modified residue3731Phosphothreonine Ref.2
Modified residue3871Phosphoserine Ref.2
Modified residue3901Phosphoserine Ref.2
Modified residue4121Phosphoserine Ref.2
Modified residue4131Phosphoserine Ref.2

Sequences

Sequence LengthMass (Da)Tools
O60101 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: F40D706D5487EB4E

FASTA41443,964
        10         20         30         40         50         60 
MSLSTKLAIT DVDLKGKNVL IRVDFNVPLD GDRITNNARI VGALPTIKYA LEQQPKAVIL 

        70         80         90        100        110        120 
MSHLGRPNGA RVAKYSLKPV AAELSKLLGK PVKFLDDCVG PEVEKACKEA KGGEVILLEN 

       130        140        150        160        170        180 
LRFHIEEEGS AKVDGKKVKA DASAVEAFRK SLTSLGDIFV NDAFGTAHRA HSSMVGVDLP 

       190        200        210        220        230        240 
RVSGFLMKKE LDYFSKALEN PARPFLAILG GAKVADKIQL IDNLLDKVNR LIICGGMAFT 

       250        260        270        280        290        300 
FLKVLNGMKI GDSLFDEAGS KNVESMMAKA KKNNVEVFLP VDFVTADKFD KDAKVGSATA 

       310        320        330        340        350        360 
EEGIPDGWMG LDCGPKSSAK FAEVITTSKT IVWNGPAGVF EFDNFAKGTK SMLDACVKTC 

       370        380        390        400        410 
EAGNVVIVGG GDTATVAKKY GKEDALSHVS TGGGASLELL EGKALPGVVA LSSK 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-75; SER-76; SER-143; SER-172; SER-173; SER-183; SER-253; SER-260; THR-299; SER-328; SER-351; THR-373; SER-387; SER-390; SER-412 AND SER-413, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAA19322.1.
PIRT39450.
RefSeqNP_596730.1. NM_001022656.2.

3D structure databases

ProteinModelPortalO60101.
SMRO60101. Positions 2-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276315. 10 interactions.
IntActO60101. 2 interactions.
MINTMINT-4677183.
STRING4896.SPBC14F5.04c-1.

Proteomic databases

MaxQBO60101.
PaxDbO60101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC14F5.04c.1; SPBC14F5.04c.1:pep; SPBC14F5.04c.
GeneID2539764.
KEGGspo:SPBC14F5.04c.

Organism-specific databases

PomBaseSPBC14F5.04c.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
KOK00927.
OMAFNVPLRE.
OrthoDBEOG71K6D7.
PhylomeDBO60101.

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20800914.
PROO60101.

Entry information

Entry namePGK_SCHPO
AccessionPrimary (citable) accession number: O60101
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways