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Protein

Phosphoglycerate kinase

Gene

pgk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd3), Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1)
  2. Phosphoglycerate kinase (pgk1)
  3. Phosphoglycerate mutase (gpm1)
  4. Enolase 1-2 (eno102), Enolase 1-1 (eno101)
  5. Pyruvate kinase (pyk1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Binding sitei217ATPBy similarity1
Binding sitei310ATP; via carbonyl oxygenBy similarity1
Binding sitei341ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi370 – 373ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk1
ORF Names:SPBC14F5.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.04c.
PomBaseiSPBC14F5.04c. pgk1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001458891 – 414Phosphoglycerate kinaseAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei75Phosphotyrosine1 Publication1
Modified residuei76Phosphoserine1 Publication1
Modified residuei143Phosphoserine1 Publication1
Modified residuei172Phosphoserine1 Publication1
Modified residuei173Phosphoserine1 Publication1
Modified residuei183Phosphoserine1 Publication1
Modified residuei253Phosphoserine1 Publication1
Modified residuei260Phosphoserine1 Publication1
Modified residuei299Phosphothreonine1 Publication1
Modified residuei328Phosphoserine1 Publication1
Modified residuei351Phosphoserine1 Publication1
Modified residuei373Phosphothreonine1 Publication1
Modified residuei387Phosphoserine1 Publication1
Modified residuei390Phosphoserine1 Publication1
Modified residuei412Phosphoserine1 Publication1
Modified residuei413Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO60101.
PRIDEiO60101.

PTM databases

iPTMnetiO60101.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi276315. 8 interactors.
IntActiO60101. 2 interactors.
MINTiMINT-4677183.

Structurei

3D structure databases

ProteinModelPortaliO60101.
SMRiO60101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000227107.
InParanoidiO60101.
KOiK00927.
OMAiKKVEMAP.
OrthoDBiEOG092C2GKC.
PhylomeDBiO60101.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSTKLAIT DVDLKGKNVL IRVDFNVPLD GDRITNNARI VGALPTIKYA
60 70 80 90 100
LEQQPKAVIL MSHLGRPNGA RVAKYSLKPV AAELSKLLGK PVKFLDDCVG
110 120 130 140 150
PEVEKACKEA KGGEVILLEN LRFHIEEEGS AKVDGKKVKA DASAVEAFRK
160 170 180 190 200
SLTSLGDIFV NDAFGTAHRA HSSMVGVDLP RVSGFLMKKE LDYFSKALEN
210 220 230 240 250
PARPFLAILG GAKVADKIQL IDNLLDKVNR LIICGGMAFT FLKVLNGMKI
260 270 280 290 300
GDSLFDEAGS KNVESMMAKA KKNNVEVFLP VDFVTADKFD KDAKVGSATA
310 320 330 340 350
EEGIPDGWMG LDCGPKSSAK FAEVITTSKT IVWNGPAGVF EFDNFAKGTK
360 370 380 390 400
SMLDACVKTC EAGNVVIVGG GDTATVAKKY GKEDALSHVS TGGGASLELL
410
EGKALPGVVA LSSK
Length:414
Mass (Da):43,964
Last modified:August 1, 1998 - v1
Checksum:iF40D706D5487EB4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19322.1.
PIRiT39450.
RefSeqiNP_596730.1. NM_001022656.2.

Genome annotation databases

EnsemblFungiiSPBC14F5.04c.1; SPBC14F5.04c.1:pep; SPBC14F5.04c.
GeneIDi2539764.
KEGGispo:SPBC14F5.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19322.1.
PIRiT39450.
RefSeqiNP_596730.1. NM_001022656.2.

3D structure databases

ProteinModelPortaliO60101.
SMRiO60101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276315. 8 interactors.
IntActiO60101. 2 interactors.
MINTiMINT-4677183.

PTM databases

iPTMnetiO60101.

Proteomic databases

MaxQBiO60101.
PRIDEiO60101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14F5.04c.1; SPBC14F5.04c.1:pep; SPBC14F5.04c.
GeneIDi2539764.
KEGGispo:SPBC14F5.04c.

Organism-specific databases

EuPathDBiFungiDB:SPBC14F5.04c.
PomBaseiSPBC14F5.04c. pgk1.

Phylogenomic databases

HOGENOMiHOG000227107.
InParanoidiO60101.
KOiK00927.
OMAiKKVEMAP.
OrthoDBiEOG092C2GKC.
PhylomeDBiO60101.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.

Miscellaneous databases

PROiO60101.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_SCHPO
AccessioniPrimary (citable) accession number: O60101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.