O60087 (AMYG_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glucoamylase
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Meiotic expression up-regulated protein 17

Gene names

Name:	meu17
ORF Names:	SPBC14C8.05c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Meiosis Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from sequence orthology. Source: PomBase glycogen catabolic process Inferred from sequence orthology. Source: PomBase meiosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 16823372. Source: PomBase cell surface Non-traceable author statement. Source: PomBase endoplasmic reticulum Inferred from direct assay PubMed 16823372. Source: PomBase prospore membrane Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	glucan 1,4-alpha-glucosidase activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 28	12	By similarity		PRO_0000001479
	Chain	29 – 450	422	Probable glucoamylase		PRO_0000001480
Sites
	Active site	203	1	Proton acceptor By similarity
	Active site	206	1	Proton donor By similarity
	Binding site	147	1	Substrate By similarity
Amino acid modifications
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O60087 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 31C5BF2A8E4785FE	FASTA	450	51,164
	10 20 30 40 50 60 MRTYWLFLLL GGVVSAESLL SPNKRSKEAS MDEWTDQQKG IAMGHMLNNI GDSGMHAKDI 70 80 90 100 110 120 NPGCIIASPS TDSPDYYYQW VRDSALTIMT ILDRFFEGDK GLEPIIVKYM DEMVRLQKVP 130 140 150 160 170 180 NPSGDFYAGG LGEPKFNVDG TSYDGDWGRP QNDSPALRAI AFIKYMNYLF ENGKEVHEVT 190 200 210 220 230 240 VWIEAVLADL DYTANHWTEA SFDLWEEIKD VHYFTLAVQK RAMQDGTAFA KRIGAPDQAA 250 260 270 280 290 300 LYQRTIEPID LKLGEFWDPG MGVIKGYKGR VDRSGLDCST LLASLYSNEF DMHILPTLLK 310 320 330 340 350 360 LQETMTRDYP VNQGWKQAMG RYPEDVYDGV SKSIGNPWFI CTSSAAEIIY KAIAYYDNKG 370 380 390 400 410 420 LPELTEYNIH FFMKFAEFGD PYNWSVIRKN MHTYADNFLK AVAEFQHPNG SMSEQFSRDD 430 440 450 GHQKGARDLT WSYSSLLNAI YRREAIKGSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe." Watanabe T., Miyashita K., Saito T.T., Yoneki T., Kakihara Y., Nabeshima K., Kishi Y.A., Shimoda C., Nojima H. Nucleic Acids Res. 29:2327-2337(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 210-450. Strain: CD16-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA18423.1. AB054301 mRNA. Translation: BAB60870.1.
PIR	T39433.
RefSeq	NP_595908.1. NM_001021816.2.
3D structure databases
ProteinModelPortal	O60087.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC14C8.05c-1.
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC14C8.05c.1; SPBC14C8.05c.1:pep; SPBC14C8.05c.
GeneID	2539682.
KEGG	spo:SPBC14C8.05c.
Organism-specific databases
PomBase	SPBC14C8.05c.
Phylogenomic databases
eggNOG	COG3387.
HOGENOM	HOG000182646.
KO	K01178.
OMA	IGNPWFI.
OrthoDB	EOG4FFH90.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR000165. Glucoamylase. IPR011613. Glyco_hydro_15. [Graphical view]
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
NextBio	20800836.

Entry information

Entry name

AMYG_SCHPO

Accession

Primary (citable) accession number: O60087
Secondary accession number(s): Q96WS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program