Reviewed, UniProtKB/Swiss-Prot O60087 (AMYG_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable glucoamylase
    EC=3.2.1.3
Alternative name(s):
    Glucan 1,4-alpha-glucosidase
    1,4-alpha-D-glucan glucohydrolase
    Meiotic expression up-regulated protein 17

Gene names

Name:	meu17
ORF Names:	SPBC14C8.05c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Meiosis Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_SPombe cell surface Non-traceable author statement. Source: GeneDB_SPombe endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe prospore membrane Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 28	12	By similarity		PRO_0000001479
	Chain	29 – 450	422	Probable glucoamylase		PRO_0000001480
Sites
	Active site	203	1	Proton acceptor By similarity
	Active site	206	1	Proton donor By similarity
	Binding site	147	1	Substrate By similarity
Amino acid modifications
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O60087-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 31C5BF2A8E4785FE	FASTA	450	51,164
	10 20 30 40 50 60 MRTYWLFLLL GGVVSAESLL SPNKRSKEAS MDEWTDQQKG IAMGHMLNNI GDSGMHAKDI 70 80 90 100 110 120 NPGCIIASPS TDSPDYYYQW VRDSALTIMT ILDRFFEGDK GLEPIIVKYM DEMVRLQKVP 130 140 150 160 170 180 NPSGDFYAGG LGEPKFNVDG TSYDGDWGRP QNDSPALRAI AFIKYMNYLF ENGKEVHEVT 190 200 210 220 230 240 VWIEAVLADL DYTANHWTEA SFDLWEEIKD VHYFTLAVQK RAMQDGTAFA KRIGAPDQAA 250 260 270 280 290 300 LYQRTIEPID LKLGEFWDPG MGVIKGYKGR VDRSGLDCST LLASLYSNEF DMHILPTLLK 310 320 330 340 350 360 LQETMTRDYP VNQGWKQAMG RYPEDVYDGV SKSIGNPWFI CTSSAAEIIY KAIAYYDNKG 370 380 390 400 410 420 LPELTEYNIH FFMKFAEFGD PYNWSVIRKN MHTYADNFLK AVAEFQHPNG SMSEQFSRDD 430 440 450 GHQKGARDLT WSYSSLLNAI YRREAIKGSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe." Watanabe T., Miyashita K., Saito T.T., Yoneki T., Kakihara Y., Nabeshima K., Kishi Y.A., Shimoda C., Nojima H. Nucleic Acids Res. 29:2327-2337(2001) [PubMed: 11376151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 210-450. Strain: CD16-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA18423.1. AB054301 mRNA. Translation: BAB60870.1.
PIR	T39433.
RefSeq	NP_595908.1.
3D structure databases
HSSP	HSSP built from PDB template 1GAI based on UniProtKB P04064.
ModBase	Search...
Protein-protein interaction databases
STRING	O60087.
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Genome annotation databases
GeneID	2539682.
GenomeReviews	Gene locus meu17 in contig CU329671_GR.
KEGG	spo:SPBC14C8.05c.
NMPDR	fig|4896.1.peg.1774.
Organism-specific databases
GeneDB_Spombe	SPBC14C8.05c.
Phylogenomic databases
OMA	PQNDGPA.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003976-MON.
BRENDA	3.2.1.3. 653.
Gene expression databases
ArrayExpress	O60087.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR000165. Glyco_hydro_15. IPR011613. Glyco_hydro_15_rel. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
ProtoNet	Search...

Entry information

Entry name

AMYG_SCHPO

Accession

Primary (citable) accession number: O60087
Secondary accession number(s): Q96WS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)