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O60087

- AMYG_SCHPO

UniProt

O60087 - AMYG_SCHPO

Protein

Probable glucoamylase

Gene

meu17

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei203 – 2031Proton acceptorBy similarity
    Active sitei206 – 2061Proton donorBy similarity

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: PomBase

    GO - Biological processi

    1. ascospore formation Source: PomBase
    2. glycogen catabolic process Source: PomBase
    3. meiotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Meiosis, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH15. Glycoside Hydrolase Family 15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glucoamylase (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Meiotic expression up-regulated protein 17
    Gene namesi
    Name:meu17
    ORF Names:SPBC14C8.05c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC14C8.05c.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: PomBase
    2. endoplasmic reticulum Source: PomBase
    3. Golgi apparatus Source: PomBase
    4. prospore membrane Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 2812By similarityPRO_0000001479Add
    BLAST
    Chaini29 – 450422Probable glucoamylasePRO_0000001480Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    BioGridi276237. 1 interaction.
    MINTiMINT-4677080.
    STRINGi4896.SPBC14C8.05c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO60087.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3387.
    HOGENOMiHOG000182646.
    KOiK01178.
    OMAiWIRDSAI.
    OrthoDBiEOG7NPG4B.
    PhylomeDBiO60087.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O60087-1 [UniParc]FASTAAdd to Basket

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    MRTYWLFLLL GGVVSAESLL SPNKRSKEAS MDEWTDQQKG IAMGHMLNNI    50
    GDSGMHAKDI NPGCIIASPS TDSPDYYYQW VRDSALTIMT ILDRFFEGDK 100
    GLEPIIVKYM DEMVRLQKVP NPSGDFYAGG LGEPKFNVDG TSYDGDWGRP 150
    QNDSPALRAI AFIKYMNYLF ENGKEVHEVT VWIEAVLADL DYTANHWTEA 200
    SFDLWEEIKD VHYFTLAVQK RAMQDGTAFA KRIGAPDQAA LYQRTIEPID 250
    LKLGEFWDPG MGVIKGYKGR VDRSGLDCST LLASLYSNEF DMHILPTLLK 300
    LQETMTRDYP VNQGWKQAMG RYPEDVYDGV SKSIGNPWFI CTSSAAEIIY 350
    KAIAYYDNKG LPELTEYNIH FFMKFAEFGD PYNWSVIRKN MHTYADNFLK 400
    AVAEFQHPNG SMSEQFSRDD GHQKGARDLT WSYSSLLNAI YRREAIKGSV 450
    Length:450
    Mass (Da):51,164
    Last modified:August 1, 1998 - v1
    Checksum:i31C5BF2A8E4785FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18423.1.
    AB054301 mRNA. Translation: BAB60870.1.
    PIRiT39433.
    RefSeqiNP_595908.1. NM_001021816.2.

    Genome annotation databases

    EnsemblFungiiSPBC14C8.05c.1; SPBC14C8.05c.1:pep; SPBC14C8.05c.
    GeneIDi2539682.
    KEGGispo:SPBC14C8.05c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA18423.1 .
    AB054301 mRNA. Translation: BAB60870.1 .
    PIRi T39433.
    RefSeqi NP_595908.1. NM_001021816.2.

    3D structure databases

    ProteinModelPortali O60087.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276237. 1 interaction.
    MINTi MINT-4677080.
    STRINGi 4896.SPBC14C8.05c-1.

    Protein family/group databases

    CAZyi GH15. Glycoside Hydrolase Family 15.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC14C8.05c.1 ; SPBC14C8.05c.1:pep ; SPBC14C8.05c .
    GeneIDi 2539682.
    KEGGi spo:SPBC14C8.05c.

    Organism-specific databases

    PomBasei SPBC14C8.05c.

    Phylogenomic databases

    eggNOGi COG3387.
    HOGENOMi HOG000182646.
    KOi K01178.
    OMAi WIRDSAI.
    OrthoDBi EOG7NPG4B.
    PhylomeDBi O60087.

    Miscellaneous databases

    NextBioi 20800836.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe."
      Watanabe T., Miyashita K., Saito T.T., Yoneki T., Kakihara Y., Nabeshima K., Kishi Y.A., Shimoda C., Nojima H.
      Nucleic Acids Res. 29:2327-2337(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 210-450.
      Strain: CD16-1.

    Entry informationi

    Entry nameiAMYG_SCHPO
    AccessioniPrimary (citable) accession number: O60087
    Secondary accession number(s): Q96WS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2002
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3