Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable glucoamylase

Gene

meu17

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147SubstrateBy similarity1
Active sitei203Proton acceptorBy similarity1
Active sitei206Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Meiosis, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glucoamylase (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Meiotic expression up-regulated protein 17
Gene namesi
Name:meu17
ORF Names:SPBC14C8.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.05c.
PomBaseiSPBC14C8.05c. meu17.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: PomBase
  • fungal-type vacuole Source: GO_Central
  • Golgi apparatus Source: PomBase
  • prospore membrane Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000000147917 – 28By similarityAdd BLAST12
ChainiPRO_000000148029 – 450Probable glucoamylaseAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi383N-linked (GlcNAc...)Sequence analysis1
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO60087.

Interactioni

Protein-protein interaction databases

BioGridi276237. 1 interactor.
MINTiMINT-4677080.

Structurei

3D structure databases

ProteinModelPortaliO60087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiO60087.
KOiK01178.
OMAiRIFFLMI.
OrthoDBiEOG092C1YYH.
PhylomeDBiO60087.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O60087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTYWLFLLL GGVVSAESLL SPNKRSKEAS MDEWTDQQKG IAMGHMLNNI
60 70 80 90 100
GDSGMHAKDI NPGCIIASPS TDSPDYYYQW VRDSALTIMT ILDRFFEGDK
110 120 130 140 150
GLEPIIVKYM DEMVRLQKVP NPSGDFYAGG LGEPKFNVDG TSYDGDWGRP
160 170 180 190 200
QNDSPALRAI AFIKYMNYLF ENGKEVHEVT VWIEAVLADL DYTANHWTEA
210 220 230 240 250
SFDLWEEIKD VHYFTLAVQK RAMQDGTAFA KRIGAPDQAA LYQRTIEPID
260 270 280 290 300
LKLGEFWDPG MGVIKGYKGR VDRSGLDCST LLASLYSNEF DMHILPTLLK
310 320 330 340 350
LQETMTRDYP VNQGWKQAMG RYPEDVYDGV SKSIGNPWFI CTSSAAEIIY
360 370 380 390 400
KAIAYYDNKG LPELTEYNIH FFMKFAEFGD PYNWSVIRKN MHTYADNFLK
410 420 430 440 450
AVAEFQHPNG SMSEQFSRDD GHQKGARDLT WSYSSLLNAI YRREAIKGSV
Length:450
Mass (Da):51,164
Last modified:August 1, 1998 - v1
Checksum:i31C5BF2A8E4785FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18423.1.
AB054301 mRNA. Translation: BAB60870.1.
PIRiT39433.
RefSeqiNP_595908.1. NM_001021816.2.

Genome annotation databases

EnsemblFungiiSPBC14C8.05c.1; SPBC14C8.05c.1:pep; SPBC14C8.05c.
GeneIDi2539682.
KEGGispo:SPBC14C8.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18423.1.
AB054301 mRNA. Translation: BAB60870.1.
PIRiT39433.
RefSeqiNP_595908.1. NM_001021816.2.

3D structure databases

ProteinModelPortaliO60087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276237. 1 interactor.
MINTiMINT-4677080.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEiO60087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14C8.05c.1; SPBC14C8.05c.1:pep; SPBC14C8.05c.
GeneIDi2539682.
KEGGispo:SPBC14C8.05c.

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.05c.
PomBaseiSPBC14C8.05c. meu17.

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiO60087.
KOiK01178.
OMAiRIFFLMI.
OrthoDBiEOG092C1YYH.
PhylomeDBiO60087.

Miscellaneous databases

PROiO60087.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYG_SCHPO
AccessioniPrimary (citable) accession number: O60087
Secondary accession number(s): Q96WS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.