Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine aminopeptidase 2

Gene

fma2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei179SubstrateUniRule annotation1
Metal bindingi199Divalent metal cation 1UniRule annotation1
Metal bindingi210Divalent metal cation 1UniRule annotation1
Metal bindingi210Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi279Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei287SubstrateUniRule annotation1
Metal bindingi312Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi407Divalent metal cation 1UniRule annotation1
Metal bindingi407Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Protein family/group databases

MEROPSiM24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:fma2
Synonyms:map2
ORF Names:SPBC14C8.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.03.
PomBaseiSPBC14C8.03. fma2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003117661 – 426Methionine aminopeptidase 2Add BLAST426

Proteomic databases

MaxQBiO60085.
PRIDEiO60085.

Interactioni

Protein-protein interaction databases

BioGridi276246. 1 interactor.
MINTiMINT-4677062.

Structurei

3D structure databases

ProteinModelPortaliO60085.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi47 – 60Lys-richAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226278.
InParanoidiO60085.
KOiK01265.
OMAiWEHTILL.
OrthoDBiEOG092C3NQP.
PhylomeDBiO60085.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATTTEAT AKDLQEKLSL KENDVVEDDG KVEENDAAEE GASNGEKKKK
60 70 80 90 100
KKKKSSKKKK TPQEQTNPPT VGLSKIFVNK KYPVGEVCDY AEDNLWRTTD
110 120 130 140 150
EEKRALDRQN FDQYNDLRRA AEVHRQARQY AQSVIKPGMS MMDVVNTIEN
160 170 180 190 200
TTRALVEEDG LKSGIGFPTG VSLNHCAAHY TPNAGDTTIL KEKDVMKVDI
210 220 230 240 250
GVHVNGRIVD SAFTMSFDPQ YDNLLAAVKA ATNKGIEEAG IDARLNEIGE
260 270 280 290 300
AIQEVMESYE VEINGKTHQV KSIRNLCGHN LDPYIIHGGK SVPIVKGGEE
310 320 330 340 350
IKMEEGEIFA IETFGSTGRG VVHEDMECSH YAKIPDAGHI PLRLPRAKAL
360 370 380 390 400
LNTITQNFGT LPFCRRYLDR IGESKYLLAL NNLVSAGIVQ DYPPLCDIRG
410 420
SYTAQFEHTI ILHPTQKEVV SRGDDY
Length:426
Mass (Da):47,271
Last modified:August 1, 1998 - v1
Checksum:iE23E56582F7025D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18421.1.
PIRiT39431.
RefSeqiNP_595906.1. NM_001021814.2.

Genome annotation databases

EnsemblFungiiSPBC14C8.03.1; SPBC14C8.03.1:pep; SPBC14C8.03.
GeneIDi2539692.
KEGGispo:SPBC14C8.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18421.1.
PIRiT39431.
RefSeqiNP_595906.1. NM_001021814.2.

3D structure databases

ProteinModelPortaliO60085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276246. 1 interactor.
MINTiMINT-4677062.

Protein family/group databases

MEROPSiM24.002.

Proteomic databases

MaxQBiO60085.
PRIDEiO60085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14C8.03.1; SPBC14C8.03.1:pep; SPBC14C8.03.
GeneIDi2539692.
KEGGispo:SPBC14C8.03.

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.03.
PomBaseiSPBC14C8.03. fma2.

Phylogenomic databases

HOGENOMiHOG000226278.
InParanoidiO60085.
KOiK01265.
OMAiWEHTILL.
OrthoDBiEOG092C3NQP.
PhylomeDBiO60085.

Enzyme and pathway databases

ReactomeiR-SPO-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

PROiO60085.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP2_SCHPO
AccessioniPrimary (citable) accession number: O60085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.