Reviewed, UniProtKB/Swiss-Prot O60078 (TYR1_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable prephenate dehydrogenase [NADP+]
      Short name=PRDH
    EC=1.3.1.13

Gene names

ORF Names:

SPCC1494.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Prephenate + NADP(+) = 4-hydroxyphenylpyruvate + CO(2) + NADPH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; L-tyrosine from prephenate: step 1/2.
Sequence similarities	Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	binding Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Probable prephenate dehydrogenase [NADP+]		PRO_0000119207
Regions
	Domain	5 – 285	281	Prephenate/arogenate dehydrogenase
	Nucleotide binding	5 – 34	30	NADP Potential
Experimental info
	Sequence conflict	429 – 431	3	QKM → KKCDFLIAAFCVYNS in BAA13874. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O60078-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C598458426E620D7	FASTA	431	48,906
	10 20 30 40 50 60 MKETFQVGII GFGDMGRLYA EYISKAGWRV NVCDRPENYE SIQATYGNGG YTVLKDGFQV 70 80 90 100 110 120 SRTSDYILYS VEAEHIDKVV ALYGPATKVG AIVGGQTSCK APEMNAFEKY LPEDVDIISC 130 140 150 160 170 180 HSMHGPKVNP KSQPLVIIRH RASDEHFEIV NEILSCFKSS VVYLSAKEHD RITADTQAVT 190 200 210 220 230 240 HAAFLTMGLA WHANNQYPWE INRWCGGIEN IKMNLSMRIY SSKWHVYAGL AILNPEAQRQ 250 260 270 280 290 300 IQQYASSVTE LFKLAISGKA KEYEDRIRNA GKFVFGENMD RNSSGLLLSD ELLDQYSISN 310 320 330 340 350 360 IPKDESKRNS HLSILAIVDS WSKLGIHPQN HMICSTPLFR LWVGVSEYVF RHPGLLDSCI 370 380 390 400 410 420 YTATKHNDFS PDDLEFVVAV RSWSECVAAK DFTTYKKRFL ETQEYFRPRF EEATRVGNAM 430 ISKLLENLQK M

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-431. Strain: PR745.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA19302.1. D89213 mRNA. Translation: BAA13874.1.
PIR	T41005. T43017.
RefSeq	NP_588529.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2539274.
KEGG	spo:SPCC1494.04c.
NMPDR	fig|4896.1.peg.867.
Organism-specific databases
GeneDB_Spombe	SPCC1494.04c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000428-MON.
Gene expression databases
ArrayExpress	O60078.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd. IPR003099. Prephen_DHase. IPR012385. Prephenate_DHase_fun. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02153. PDH. 1 hit. [Graphical view]
PIRSF	PIRSF036510. PDH_fung. 1 hit.
PROSITE	PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYR1_SCHPO

Accession

Primary (citable) accession number: O60078
Secondary accession number(s): P78863

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)