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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

alg5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-480985. Synthesis of dolichyl-phosphate-glucose.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5
Gene namesi
Name:alg5
ORF Names:SPBC56F2.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC56F2.10c.
PomBaseiSPBC56F2.10c. alg5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11CytoplasmicBy similarity
Transmembranei2 – 2221Helical; Signal-anchor for type II membrane proteinAdd
BLAST
Topological domaini23 – 322300LumenalBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Dolichyl-phosphate beta-glucosyltransferasePRO_0000311759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO60061.

Interactioni

Protein-protein interaction databases

BioGridi277568. 52 interactions.
MINTiMINT-4676849.

Structurei

3D structure databases

ProteinModelPortaliO60061.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000283250.
InParanoidiO60061.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG77WWPJ.
PhylomeDBiO60061.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O60061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVYIVLYTC LAGFILLFLV YYYLTHSHCP RKQLEGEETC VFIENGQKKS
60 70 80 90 100
LTLEKWSTSD NIQITVIVPA YNESKRIGNM LQETVDHLEK YYRSSSSAGQ
110 120 130 140 150
RRWEILIVDD ESKDTTVNAV LEFSNKLDLR DHLRVCSLKR NRGKGGAVTW
160 170 180 190 200
GMLYARGQYA IFADADGASQ FSDLELLFKN MPPGPRGGVV VGSRAHMVNT
210 220 230 240 250
AAVVKRSFIR NFLMHCFHKL LQILGIREIG DTQCGFKLFS REAYQSIFPR
260 270 280 290 300
MHVEGWIFDI EVLTLARFFG LPIIEVPITW HEVGGSKMTL LKDSISMAID
310 320
LLVIRLNYTF GIWERPSAKR IT
Length:322
Mass (Da):36,774
Last modified:August 1, 1998 - v1
Checksum:iB97B2EF2C8D872C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18889.1.
PIRiT40534.
RefSeqiNP_596707.1. NM_001022632.2.

Genome annotation databases

EnsemblFungiiSPBC56F2.10c.1; SPBC56F2.10c.1:pep; SPBC56F2.10c.
GeneIDi2541053.
KEGGispo:SPBC56F2.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA18889.1.
PIRiT40534.
RefSeqiNP_596707.1. NM_001022632.2.

3D structure databases

ProteinModelPortaliO60061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277568. 52 interactions.
MINTiMINT-4676849.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

MaxQBiO60061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC56F2.10c.1; SPBC56F2.10c.1:pep; SPBC56F2.10c.
GeneIDi2541053.
KEGGispo:SPBC56F2.10c.

Organism-specific databases

EuPathDBiFungiDB:SPBC56F2.10c.
PomBaseiSPBC56F2.10c. alg5.

Phylogenomic databases

HOGENOMiHOG000283250.
InParanoidiO60061.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG77WWPJ.
PhylomeDBiO60061.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-480985. Synthesis of dolichyl-phosphate-glucose.

Miscellaneous databases

PROiO60061.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALG5_SCHPO
AccessioniPrimary (citable) accession number: O60061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.